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The map label for this gene is kinE [H]

Identifier: 197117567

GI number: 197117567

Start: 1372883

End: 1375216

Strand: Direct

Name: kinE [H]

Synonym: Gbem_1179

Alternate gene names: 197117567

Gene position: 1372883-1375216 (Clockwise)

Preceding gene: 197117564

Following gene: 197117568

Centisome position: 29.75

GC content: 65.55

Gene sequence:

>2334_bases
ATGATCCTCCCTCAAAAACTCTCGACCTTCCGCTCCAGCTTTCAGTTCAAGCTTTTGCTCCGCTTCACGCTCTTCGCCGC
GGCCATCACCGTTCTCTGCGTTACCCTCTTCGTCATCTACCAGATCAAAGAAGACAAGCGGCACGTCTCCGAGCAGCTCC
GGCTCGAGGCCCGCACCCTGGCCAGCTCCATCCGCCTTCCCCTGTACGCGGAGAACATGGAACTCCTCGACTGGTACGCG
CGGGAAACGCTGCGGCTCCCCGGGATCCGCTGCGTGGAGATAGTGGGGGGAAACGGCAAGTTGCAGGTCAGGGTTCCGGC
TCTTCCCCCCGCCGGGGAGGAGCTGATCGTAGAGGGGGCGCAGGTGCGCACCCTGCCGCTGCACCTGTCGCAGGACCAGT
CGCTGGAGGCGGCGGCGGGAGACCGCATCGGGGAGGTACGTCTTTATCGCGGCACCGACGACCTCTGGCAGGCGGGACGC
AGGCTCGCCTGGTACTCGTGCCTGCTCGCCTTCGGCTGCTGGCTCTTGGTCTCCTTTTCCTGCTACCTGCTGCTGCGGCG
GGTCACCGCGTCGTTCAACTCGCTGATGCGCGGGCTGAAGAACGTCCACGGCGGCGATTACGTCTCCCGCATCGCGGTCA
CCTCCGACGACGAGCCGGGAAGGGCATCGGCCTCGGTGAACGAGCTGGCCGAATCGCTCAGGCAGCGCGAACAGGAGAAC
CGCCGGCTGCACAAGGAACTTTTGGCTTCGATGGATTTCGAGATTGCCTCGAAGAGGCAGCTGGTATCCATCAACGCGGA
CCTGGAAAAGGAGATCGCGGAACGAAGCCGCGCGCGCCAGGAGCTGAAGAACCTGGTGGAGCAGTTGCCTTTGGGGATCA
TCTGGTCCGACGACGACGGCGCCATTGAGTACCTGAACGCCTTCGTGGCGCAGAGAATCGGCTACCGCGACGCCCGCAAC
GTCGACGAGTGGCTCTCCCGGGTCTGTCCCGATCCCTGCTACCGCGACCGCGTCGCCGCGCAAAGGCGCGCCGCCATCGC
CGGCTGGGAGCGGGATAACCAGGTGACCTTCTACGAGGTGAAGGCGGTATGCAGCGACGGCTCGCAGCGCCAGCTGAACT
GCAGCAACCAGCGCTCCGGCTCGCGCACCGTCGACATCGTCATAGACCTGACCGATCGGGAGCTTTTGCAGCAGCAGATC
GTCAGGAACCAGAAGCTCGAGTCCATCGGGGTGCTGGCGGGGGGGGTCGCACACAACTTCAACAACGCCCTGACCGGGGT
CCTTGGCTACATCTCCTTCGCCAAGAAGTACCTGGACCAGTCCCACGCCGCGCACCCGCTGCTGTTGCACGCGGAGAAGG
CGACCTTGCGCGCGGCCGGTTTCGCGAACCAGCTCCTGAGCTTCGCCAAAGGGGGCGCGCCCCTGAGAAAGGCGGTGCCG
GTCGCCAGGATAGTCGAGGAAAGCGTGCAGCTTGCCACAAGCGGCTCCAACGTAACGGCGGCCGTCGAGCTTCCCGCCGA
CCTCCCTCCGGTGTACGTGGACGACGGGCAGATGCGCCAGGCCTTCAACTGCATCTGCATCAACGCGGTGCAGGCGATGC
CCGACGGGGGGATGCTGACCGTGACCGGCAGGAGCGTCGCTTCCGCCCACGAGGGTCTCCCGGCGCCGCTTTGCGGCAGC
TACGTCGAGTTGAGCTTCCGGGACCAGGGGTGCGGCATCCGTGACGAGGACAAGTCCAACATCTTCACCCCCTACTTCAC
CACCAAGGCCGAGCTTGGGACGGGGCTCGGGCTTGCCACGGTGCACTCCATCATCACCAGGCACGACGGGGCGATCACCT
TCGACACCGCGCTCGGCAGGGGGACCACCTTCACCCTCTACCTCCCCGTATTCAGCACGGAGGCGCAGCCGCAGCCGCAG
CCCGCCAAGCCGCTTACCGCGGCCGCCATCCTGGGTCGCGGTGTCCTGGTCATGGACGACGAGGAGATGATCCGCAACCT
TGCCATCCAGGTCCTCTCCTCCCAGGGATACCGGGTCACCGTCTGCGCCAACGGCGAGGAAGCCGCCGCACTTTACGGCG
AAGCCTACCGCTCCGGGACCCCTTTTCTTGCGGCCATCCTCGACTTGACCGTCGCCAGCGGCAGCGGAGGGAAGGAGGCG
GCCCGCCAGATCCTCGCCATCGACCCGCAGGCCCGGCTCATCGTCTCCAGCGGCTACAGCAACGACATCGTGATGAACCG
CTACCGGGATTACGGCTTCTGCGCCGCCTCCCCCAAGCCCTACGACGCCCATGAACTGTTCCGGCTCCTGAAGGAACTGG
CAGCGCAGGGGTAA

Upstream 100 bases:

>100_bases
CCTTGACTGTTCCGCATTCCGCGGGATAGACCTGGTTCAGTTTTGGAAATGGCGACGCGGCAACCATACCTGCCCATGAG
AGATGCCTACGGAGACCGGC

Downstream 100 bases:

>100_bases
CGCGGGCGGGGGCGTGAGCTACGGCATCGTCAAGCGCCACGGCGGGGAAGGGGACCGAGGTGACCGTCACCGTGCCGCTG
CGACAGCCGAAAGGCGGAAG

Product: HAMP domain-containing sensor histidine kinase response regulator

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 777; Mature: 777

Protein sequence:

>777_residues
MILPQKLSTFRSSFQFKLLLRFTLFAAAITVLCVTLFVIYQIKEDKRHVSEQLRLEARTLASSIRLPLYAENMELLDWYA
RETLRLPGIRCVEIVGGNGKLQVRVPALPPAGEELIVEGAQVRTLPLHLSQDQSLEAAAGDRIGEVRLYRGTDDLWQAGR
RLAWYSCLLAFGCWLLVSFSCYLLLRRVTASFNSLMRGLKNVHGGDYVSRIAVTSDDEPGRASASVNELAESLRQREQEN
RRLHKELLASMDFEIASKRQLVSINADLEKEIAERSRARQELKNLVEQLPLGIIWSDDDGAIEYLNAFVAQRIGYRDARN
VDEWLSRVCPDPCYRDRVAAQRRAAIAGWERDNQVTFYEVKAVCSDGSQRQLNCSNQRSGSRTVDIVIDLTDRELLQQQI
VRNQKLESIGVLAGGVAHNFNNALTGVLGYISFAKKYLDQSHAAHPLLLHAEKATLRAAGFANQLLSFAKGGAPLRKAVP
VARIVEESVQLATSGSNVTAAVELPADLPPVYVDDGQMRQAFNCICINAVQAMPDGGMLTVTGRSVASAHEGLPAPLCGS
YVELSFRDQGCGIRDEDKSNIFTPYFTTKAELGTGLGLATVHSIITRHDGAITFDTALGRGTTFTLYLPVFSTEAQPQPQ
PAKPLTAAAILGRGVLVMDDEEMIRNLAIQVLSSQGYRVTVCANGEEAAALYGEAYRSGTPFLAAILDLTVASGSGGKEA
ARQILAIDPQARLIVSSGYSNDIVMNRYRDYGFCAASPKPYDAHELFRLLKELAAQG

Sequences:

>Translated_777_residues
MILPQKLSTFRSSFQFKLLLRFTLFAAAITVLCVTLFVIYQIKEDKRHVSEQLRLEARTLASSIRLPLYAENMELLDWYA
RETLRLPGIRCVEIVGGNGKLQVRVPALPPAGEELIVEGAQVRTLPLHLSQDQSLEAAAGDRIGEVRLYRGTDDLWQAGR
RLAWYSCLLAFGCWLLVSFSCYLLLRRVTASFNSLMRGLKNVHGGDYVSRIAVTSDDEPGRASASVNELAESLRQREQEN
RRLHKELLASMDFEIASKRQLVSINADLEKEIAERSRARQELKNLVEQLPLGIIWSDDDGAIEYLNAFVAQRIGYRDARN
VDEWLSRVCPDPCYRDRVAAQRRAAIAGWERDNQVTFYEVKAVCSDGSQRQLNCSNQRSGSRTVDIVIDLTDRELLQQQI
VRNQKLESIGVLAGGVAHNFNNALTGVLGYISFAKKYLDQSHAAHPLLLHAEKATLRAAGFANQLLSFAKGGAPLRKAVP
VARIVEESVQLATSGSNVTAAVELPADLPPVYVDDGQMRQAFNCICINAVQAMPDGGMLTVTGRSVASAHEGLPAPLCGS
YVELSFRDQGCGIRDEDKSNIFTPYFTTKAELGTGLGLATVHSIITRHDGAITFDTALGRGTTFTLYLPVFSTEAQPQPQ
PAKPLTAAAILGRGVLVMDDEEMIRNLAIQVLSSQGYRVTVCANGEEAAALYGEAYRSGTPFLAAILDLTVASGSGGKEA
ARQILAIDPQARLIVSSGYSNDIVMNRYRDYGFCAASPKPYDAHELFRLLKELAAQG
>Mature_777_residues
MILPQKLSTFRSSFQFKLLLRFTLFAAAITVLCVTLFVIYQIKEDKRHVSEQLRLEARTLASSIRLPLYAENMELLDWYA
RETLRLPGIRCVEIVGGNGKLQVRVPALPPAGEELIVEGAQVRTLPLHLSQDQSLEAAAGDRIGEVRLYRGTDDLWQAGR
RLAWYSCLLAFGCWLLVSFSCYLLLRRVTASFNSLMRGLKNVHGGDYVSRIAVTSDDEPGRASASVNELAESLRQREQEN
RRLHKELLASMDFEIASKRQLVSINADLEKEIAERSRARQELKNLVEQLPLGIIWSDDDGAIEYLNAFVAQRIGYRDARN
VDEWLSRVCPDPCYRDRVAAQRRAAIAGWERDNQVTFYEVKAVCSDGSQRQLNCSNQRSGSRTVDIVIDLTDRELLQQQI
VRNQKLESIGVLAGGVAHNFNNALTGVLGYISFAKKYLDQSHAAHPLLLHAEKATLRAAGFANQLLSFAKGGAPLRKAVP
VARIVEESVQLATSGSNVTAAVELPADLPPVYVDDGQMRQAFNCICINAVQAMPDGGMLTVTGRSVASAHEGLPAPLCGS
YVELSFRDQGCGIRDEDKSNIFTPYFTTKAELGTGLGLATVHSIITRHDGAITFDTALGRGTTFTLYLPVFSTEAQPQPQ
PAKPLTAAAILGRGVLVMDDEEMIRNLAIQVLSSQGYRVTVCANGEEAAALYGEAYRSGTPFLAAILDLTVASGSGGKEA
ARQILAIDPQARLIVSSGYSNDIVMNRYRDYGFCAASPKPYDAHELFRLLKELAAQG

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=34.5238095238095, Blast_Score=133, Evalue=6e-32,
Organism=Escherichia coli, GI1788549, Length=249, Percent_Identity=32.5301204819277, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=26.2108262108262, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=292, Percent_Identity=23.972602739726, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 85402; Mature: 85402

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50885 HAMP ; PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPQKLSTFRSSFQFKLLLRFTLFAAAITVLCVTLFVIYQIKEDKRHVSEQLRLEARTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASSIRLPLYAENMELLDWYARETLRLPGIRCVEIVGGNGKLQVRVPALPPAGEELIVEGA
HHHCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHEECCC
QVRTLPLHLSQDQSLEAAAGDRIGEVRLYRGTDDLWQAGRRLAWYSCLLAFGCWLLVSFS
EEEEEEEECCCCCCCHHHCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
CYLLLRRVTASFNSLMRGLKNVHGGDYVSRIAVTSDDEPGRASASVNELAESLRQREQEN
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RRLHKELLASMDFEIASKRQLVSINADLEKEIAERSRARQELKNLVEQLPLGIIWSDDDG
HHHHHHHHHHCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
AIEYLNAFVAQRIGYRDARNVDEWLSRVCPDPCYRDRVAAQRRAAIAGWERDNQVTFYEV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
KAVCSDGSQRQLNCSNQRSGSRTVDIVIDLTDRELLQQQIVRNQKLESIGVLAGGVAHNF
HHHHCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHCCHHHHHH
NNALTGVLGYISFAKKYLDQSHAAHPLLLHAEKATLRAAGFANQLLSFAKGGAPLRKAVP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VARIVEESVQLATSGSNVTAAVELPADLPPVYVDDGQMRQAFNCICINAVQAMPDGGMLT
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCHHHHHHHHHEEEHHHHCCCCCEEE
VTGRSVASAHEGLPAPLCGSYVELSFRDQGCGIRDEDKSNIFTPYFTTKAELGTGLGLAT
EECCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEECCEEECHHHHCCCCHHHH
VHSIITRHDGAITFDTALGRGTTFTLYLPVFSTEAQPQPQPAKPLTAAAILGRGVLVMDD
HHHHHHHCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEEECC
EEMIRNLAIQVLSSQGYRVTVCANGEEAAALYGEAYRSGTPFLAAILDLTVASGSGGKEA
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHH
ARQILAIDPQARLIVSSGYSNDIVMNRYRDYGFCAASPKPYDAHELFRLLKELAAQG
HHHHEEECCCCEEEEECCCCCCHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILPQKLSTFRSSFQFKLLLRFTLFAAAITVLCVTLFVIYQIKEDKRHVSEQLRLEARTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASSIRLPLYAENMELLDWYARETLRLPGIRCVEIVGGNGKLQVRVPALPPAGEELIVEGA
HHHCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHEECCC
QVRTLPLHLSQDQSLEAAAGDRIGEVRLYRGTDDLWQAGRRLAWYSCLLAFGCWLLVSFS
EEEEEEEECCCCCCCHHHCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
CYLLLRRVTASFNSLMRGLKNVHGGDYVSRIAVTSDDEPGRASASVNELAESLRQREQEN
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
RRLHKELLASMDFEIASKRQLVSINADLEKEIAERSRARQELKNLVEQLPLGIIWSDDDG
HHHHHHHHHHCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
AIEYLNAFVAQRIGYRDARNVDEWLSRVCPDPCYRDRVAAQRRAAIAGWERDNQVTFYEV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
KAVCSDGSQRQLNCSNQRSGSRTVDIVIDLTDRELLQQQIVRNQKLESIGVLAGGVAHNF
HHHHCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHCCHHHHHH
NNALTGVLGYISFAKKYLDQSHAAHPLLLHAEKATLRAAGFANQLLSFAKGGAPLRKAVP
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VARIVEESVQLATSGSNVTAAVELPADLPPVYVDDGQMRQAFNCICINAVQAMPDGGMLT
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCHHHHHHHHHEEEHHHHCCCCCEEE
VTGRSVASAHEGLPAPLCGSYVELSFRDQGCGIRDEDKSNIFTPYFTTKAELGTGLGLAT
EECCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEECCEEECHHHHCCCCHHHH
VHSIITRHDGAITFDTALGRGTTFTLYLPVFSTEAQPQPQPAKPLTAAAILGRGVLVMDD
HHHHHHHCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEEECC
EEMIRNLAIQVLSSQGYRVTVCANGEEAAALYGEAYRSGTPFLAAILDLTVASGSGGKEA
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHH
ARQILAIDPQARLIVSSGYSNDIVMNRYRDYGFCAASPKPYDAHELFRLLKELAAQG
HHHHEEECCCCEEEEECCCCCCHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]