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The map label for this gene is asnB-3 [H]

Identifier: 197117470

GI number: 197117470

Start: 1256307

End: 1258289

Strand: Direct

Name: asnB-3 [H]

Synonym: Gbem_1081

Alternate gene names: 197117470

Gene position: 1256307-1258289 (Clockwise)

Preceding gene: 197117467

Following gene: 197117471

Centisome position: 27.22

GC content: 61.52

Gene sequence:

>1983_bases
TTGTGCGGTATCGCCGGAATTGTTGCTGGGGCGGGTAAAGCGGCACCGGACCTGGAGCGGATCGTCGCCATGATCTCGCC
GTTGCGTTATCGCGGCCCGGACGAGTCCGGTGTCTATCTGGACCGCCGCGCCGCGCTGGGGCACCTGCGCCTGAGCATTA
TCGGCATAGACGGCGGGATCCAGCCCATCGGCAATGAAACGGGCAAGCTCTGGATCGTCTACAACGGCGAAGCATACAAC
TACCTGGAACTTCGGGAAGAGCTGGAGGCGAAAGGGCATCGCTTCACCACCCGGACCGACACTGAAGTGCTGCTGCACCT
CTACGAAGAGTATGGCGCCGACTTCCTGTCCAGGATCAACGGCCAGTTCGCACTCGCCATATGGGACAGCGCGAAAGAGG
AACTCTTCCTCGCCAGGGACCGGGTCGGCATCAGGCCCCTTTACTACGGTTGGTCTCGCGAAGGGAGCTTCTTGTTCGCC
TCTGAGGTGAAAGCCATTTTGGCAGTAGACGGTAGCCGGGAGTTGGACCTCGAGGCTCTTGGACAGCTCTTCGTCTTCTG
GACCACGCTGCCGGGAAGGACCTTCTTCCGGGGGATTCAGGAAGTTCCCCCGGGGCATTACCTTGTCGTCGGCAAAGGTG
AGTCGGTGCCGCGCCCCTACTGGCGCATTCCGTTCCATGCTCCCGAGGAGTGCTGCGCTTTCGGCCTTGCGGAGACGGCT
GAAGCGCTGCGGGAGCTTCTCAGCGACGCGGTGCGCCTCAGGCTGCGCTCAGACGTCCCGGTGGGGGCGTACCTAAGCGG
CGGGCTCGATTCTTCCGTGATTACAACGCTCATTCAGCGCCATTGCGACAGCCATCTCAAGACCTTCTCGCTTGCCTTCC
AGGACGGCATTTTTGACGAGAGCGCTGCGCAGCGGGAGATGGTGCGTTTCCTGGGGAGCGACAACCGGCAGGTTATGGTG
GAGAACGGCGACATCCGCCGCTTCTTTCCGGAGACGGTATGGCACTGTGAAAAGCCGACGCTGCGAACCTCGCCGGTGCC
TATGTTCCTGCTGTCGAGGCTGGTGCGTGAGGAGGGTTACAAGGTGGTGCTCTCCGGCGAGGGGGCGGACGAGATCCTCG
GGGGGTACAACATCTTCAAGGAAGCGAAAATCCGCCAGTACTGGGCGAAATGCCCGGACTCTTCCCGGCGCCCGCTGCTT
TTGGAGAGGCTCTACCCTTACATCTTCAGGAACCCCGGGCGCGGCCGTAGCTTCCTGCAGGGGTTTTTCGCTGTGTCGCG
GGAGCAGATGCACGACCCGTTTTTTTCCCATGCGGTGCGTTGGCACGCCGGGGAGCGCAACCTCGCGTTCTTGGCGCCGG
ACTGCCTGGCCGCCCTGTCGCAATACCGGCCCATGGAGGAACTGGCCCGGTGGCTCCCGGACAATTTCCTGCACCGGGAC
CTTTTGTCCCGGGCCCAAGTGCTGGAGATGGAGATCTTTCTCTCCAACTTCCTTCTCTCCTCACAAGGAGACCGGGTCGC
CATGGCCCACTCGGTCGAGATGCGTCACCCCTTTCTGGATTACCGGGTGGTGGATTTCGCTTTCCGTCTGCCGGCCAAAT
GGAAGGTGCGGGGCCTGACTGAGAAGTTCCTGCTAAGGCACGCCTTCCGGGGAATGTTGCCAGAAGGAATCGCCAGCAGG
GCGAAGCACCCGTACCGGGCCCCGATCAGCGAGCTCTTCCAAGGCACGGTTCCTCTCGATTACGTGGACGAGTTGCTGTC
GCAGCAGAGCCTGAAGCAAAGCGGTTATTTCAATGCTGAAAAGATCGGCCGCCTCTACTTGAAGGTCAAGGGTGCGCAAC
CCGGCGCCATCGGCGAATTCGAGAACATGGCGCTCATCGGCGCACTTTCAACCGAGATACTGCATCGGCAGTTCATTGCC
GGGTTTCCCTTGCGGCGGAGCTCCTTGCTGCAACCGGACTTGGTACGGTACGGGAGCCTCTGA

Upstream 100 bases:

>100_bases
GTATAGTTTAACGTTCGTTTTTTCGGGAGACGGAAGTTGCTGATTTCCCTGGCAGGATTCGCCTGCTGTTCGCTGCTCCC
GGCAGCTTCGGAGGTCGATT

Downstream 100 bases:

>100_bases
AAAAGGAGTCTGTTATGGAGCCCGCGGTACACAACCTCTTGGAAAACAGCGCAAGAATTCGCCCCGACAAGGTCGCACTG
GTGCAAGGGGACCTGCGCAT

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MCGIAGIVAGAGKAAPDLERIVAMISPLRYRGPDESGVYLDRRAALGHLRLSIIGIDGGIQPIGNETGKLWIVYNGEAYN
YLELREELEAKGHRFTTRTDTEVLLHLYEEYGADFLSRINGQFALAIWDSAKEELFLARDRVGIRPLYYGWSREGSFLFA
SEVKAILAVDGSRELDLEALGQLFVFWTTLPGRTFFRGIQEVPPGHYLVVGKGESVPRPYWRIPFHAPEECCAFGLAETA
EALRELLSDAVRLRLRSDVPVGAYLSGGLDSSVITTLIQRHCDSHLKTFSLAFQDGIFDESAAQREMVRFLGSDNRQVMV
ENGDIRRFFPETVWHCEKPTLRTSPVPMFLLSRLVREEGYKVVLSGEGADEILGGYNIFKEAKIRQYWAKCPDSSRRPLL
LERLYPYIFRNPGRGRSFLQGFFAVSREQMHDPFFSHAVRWHAGERNLAFLAPDCLAALSQYRPMEELARWLPDNFLHRD
LLSRAQVLEMEIFLSNFLLSSQGDRVAMAHSVEMRHPFLDYRVVDFAFRLPAKWKVRGLTEKFLLRHAFRGMLPEGIASR
AKHPYRAPISELFQGTVPLDYVDELLSQQSLKQSGYFNAEKIGRLYLKVKGAQPGAIGEFENMALIGALSTEILHRQFIA
GFPLRRSSLLQPDLVRYGSL

Sequences:

>Translated_660_residues
MCGIAGIVAGAGKAAPDLERIVAMISPLRYRGPDESGVYLDRRAALGHLRLSIIGIDGGIQPIGNETGKLWIVYNGEAYN
YLELREELEAKGHRFTTRTDTEVLLHLYEEYGADFLSRINGQFALAIWDSAKEELFLARDRVGIRPLYYGWSREGSFLFA
SEVKAILAVDGSRELDLEALGQLFVFWTTLPGRTFFRGIQEVPPGHYLVVGKGESVPRPYWRIPFHAPEECCAFGLAETA
EALRELLSDAVRLRLRSDVPVGAYLSGGLDSSVITTLIQRHCDSHLKTFSLAFQDGIFDESAAQREMVRFLGSDNRQVMV
ENGDIRRFFPETVWHCEKPTLRTSPVPMFLLSRLVREEGYKVVLSGEGADEILGGYNIFKEAKIRQYWAKCPDSSRRPLL
LERLYPYIFRNPGRGRSFLQGFFAVSREQMHDPFFSHAVRWHAGERNLAFLAPDCLAALSQYRPMEELARWLPDNFLHRD
LLSRAQVLEMEIFLSNFLLSSQGDRVAMAHSVEMRHPFLDYRVVDFAFRLPAKWKVRGLTEKFLLRHAFRGMLPEGIASR
AKHPYRAPISELFQGTVPLDYVDELLSQQSLKQSGYFNAEKIGRLYLKVKGAQPGAIGEFENMALIGALSTEILHRQFIA
GFPLRRSSLLQPDLVRYGSL
>Mature_660_residues
MCGIAGIVAGAGKAAPDLERIVAMISPLRYRGPDESGVYLDRRAALGHLRLSIIGIDGGIQPIGNETGKLWIVYNGEAYN
YLELREELEAKGHRFTTRTDTEVLLHLYEEYGADFLSRINGQFALAIWDSAKEELFLARDRVGIRPLYYGWSREGSFLFA
SEVKAILAVDGSRELDLEALGQLFVFWTTLPGRTFFRGIQEVPPGHYLVVGKGESVPRPYWRIPFHAPEECCAFGLAETA
EALRELLSDAVRLRLRSDVPVGAYLSGGLDSSVITTLIQRHCDSHLKTFSLAFQDGIFDESAAQREMVRFLGSDNRQVMV
ENGDIRRFFPETVWHCEKPTLRTSPVPMFLLSRLVREEGYKVVLSGEGADEILGGYNIFKEAKIRQYWAKCPDSSRRPLL
LERLYPYIFRNPGRGRSFLQGFFAVSREQMHDPFFSHAVRWHAGERNLAFLAPDCLAALSQYRPMEELARWLPDNFLHRD
LLSRAQVLEMEIFLSNFLLSSQGDRVAMAHSVEMRHPFLDYRVVDFAFRLPAKWKVRGLTEKFLLRHAFRGMLPEGIASR
AKHPYRAPISELFQGTVPLDYVDELLSQQSLKQSGYFNAEKIGRLYLKVKGAQPGAIGEFENMALIGALSTEILHRQFIA
GFPLRRSSLLQPDLVRYGSL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=409, Percent_Identity=28.361858190709, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI168229252, Length=409, Percent_Identity=28.361858190709, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI168229250, Length=409, Percent_Identity=28.361858190709, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI296010848, Length=382, Percent_Identity=28.7958115183246, Blast_Score=144, Evalue=4e-34,
Organism=Homo sapiens, GI296010852, Length=311, Percent_Identity=28.6173633440514, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI296010850, Length=311, Percent_Identity=28.6173633440514, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786889, Length=398, Percent_Identity=33.6683417085427, Blast_Score=195, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25147557, Length=397, Percent_Identity=30.4785894206549, Blast_Score=165, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI71993933, Length=397, Percent_Identity=30.4785894206549, Blast_Score=165, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI25147560, Length=329, Percent_Identity=28.2674772036474, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321563, Length=395, Percent_Identity=31.1392405063291, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325403, Length=393, Percent_Identity=30.2798982188295, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553209, Length=406, Percent_Identity=34.4827586206897, Blast_Score=198, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 74837; Mature: 74837

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGIVAGAGKAAPDLERIVAMISPLRYRGPDESGVYLDRRAALGHLRLSIIGIDGGI
CCCCHHHHCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCEEEHHHHHCEEEEEEEEECCCC
QPIGNETGKLWIVYNGEAYNYLELREELEAKGHRFTTRTDTEVLLHLYEEYGADFLSRIN
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCHHHHHHCC
GQFALAIWDSAKEELFLARDRVGIRPLYYGWSREGSFLFASEVKAILAVDGSRELDLEAL
CCEEEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHCCEEEEECCCCCCCHHHH
GQLFVFWTTLPGRTFFRGIQEVPPGHYLVVGKGESVPRPYWRIPFHAPEECCAFGLAETA
HHHHEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHCHHHHH
EALRELLSDAVRLRLRSDVPVGAYLSGGLDSSVITTLIQRHCDSHLKTFSLAFQDGIFDE
HHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SAAQREMVRFLGSDNRQVMVENGDIRRFFPETVWHCEKPTLRTSPVPMFLLSRLVREEGY
HHHHHHHHHHHCCCCCEEEEECCCCHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
KVVLSGEGADEILGGYNIFKEAKIRQYWAKCPDSSRRPLLLERLYPYIFRNPGRGRSFLQ
EEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
GFFAVSREQMHDPFFSHAVRWHAGERNLAFLAPDCLAALSQYRPMEELARWLPDNFLHRD
HHHHHHHHHHCCHHHHHHHHEECCCCCEEEECHHHHHHHHHCCCHHHHHHHCCHHHHHHH
LLSRAQVLEMEIFLSNFLLSSQGDRVAMAHSVEMRHPFLDYRVVDFAFRLPAKWKVRGLT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCEEECCHH
EKFLLRHAFRGMLPEGIASRAKHPYRAPISELFQGTVPLDYVDELLSQQSLKQSGYFNAE
HHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHH
KIGRLYLKVKGAQPGAIGEFENMALIGALSTEILHRQFIAGFPLRRSSLLQPDLVRYGSL
HHCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MCGIAGIVAGAGKAAPDLERIVAMISPLRYRGPDESGVYLDRRAALGHLRLSIIGIDGGI
CCCCHHHHCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCEEEHHHHHCEEEEEEEEECCCC
QPIGNETGKLWIVYNGEAYNYLELREELEAKGHRFTTRTDTEVLLHLYEEYGADFLSRIN
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCHHHHHHCC
GQFALAIWDSAKEELFLARDRVGIRPLYYGWSREGSFLFASEVKAILAVDGSRELDLEAL
CCEEEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHCCEEEEECCCCCCCHHHH
GQLFVFWTTLPGRTFFRGIQEVPPGHYLVVGKGESVPRPYWRIPFHAPEECCAFGLAETA
HHHHEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHCHHHHH
EALRELLSDAVRLRLRSDVPVGAYLSGGLDSSVITTLIQRHCDSHLKTFSLAFQDGIFDE
HHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SAAQREMVRFLGSDNRQVMVENGDIRRFFPETVWHCEKPTLRTSPVPMFLLSRLVREEGY
HHHHHHHHHHHCCCCCEEEEECCCCHHHCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
KVVLSGEGADEILGGYNIFKEAKIRQYWAKCPDSSRRPLLLERLYPYIFRNPGRGRSFLQ
EEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
GFFAVSREQMHDPFFSHAVRWHAGERNLAFLAPDCLAALSQYRPMEELARWLPDNFLHRD
HHHHHHHHHHCCHHHHHHHHEECCCCCEEEECHHHHHHHHHCCCHHHHHHHCCHHHHHHH
LLSRAQVLEMEIFLSNFLLSSQGDRVAMAHSVEMRHPFLDYRVVDFAFRLPAKWKVRGLT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCEEECCHH
EKFLLRHAFRGMLPEGIASRAKHPYRAPISELFQGTVPLDYVDELLSQQSLKQSGYFNAE
HHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHH
KIGRLYLKVKGAQPGAIGEFENMALIGALSTEILHRQFIAGFPLRRSSLLQPDLVRYGSL
HHCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]