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The map label for this gene is ywbI [H]

Identifier: 197117426

GI number: 197117426

Start: 1207391

End: 1208293

Strand: Direct

Name: ywbI [H]

Synonym: Gbem_1036

Alternate gene names: 197117426

Gene position: 1207391-1208293 (Clockwise)

Preceding gene: 197117422

Following gene: 308535212

Centisome position: 26.16

GC content: 62.02

Gene sequence:

>903_bases
ATGGACGTCAGGCAATTGAGGTATTTCATCGAGGTGGCCAAGGTGAAGAATTTCACCAAAGCCGCGGAGGCGCTGCACAT
AGCGCAGCCGGCGGTAAGCATGGCGGTGAAGAAGCTGGAGCAGGAGCTGGAGCTCTCCCTCTTCAACCGGCAGGACAAGA
AGGTCTCGCTCACCGCGGAGGGGGAGATCTTCCTTCCGCATGCAAAACGCATCCTCGACGATCTGAGGGCGGCCGAACTG
GAGATGGCGGAGCTGAAAGGGCTCAGCAAGGGGGAGGTGAGGCTCGGCATCCCCCCCATGATCAGCGCCTATTTCTTCCC
CGACATCATCCGGGACTTCAAGAAAAGCTACCCGCAGCTGCACCTTTCGGTCTTGGGCGAGGGGGCCTGGCGCATCCAGA
AGATGATCGGTCAAGGGGAGCTGGACATGGGGGTGGTGGCGGGGGGGAACTTCCCTGAGACGCTGGAGGTGCGCCGCTTC
CTGCGGGAGGAGATCGTGATCTGCGTGCCGCCGGATCACCCCTTCGCCCAGCGTAAGTCGGTGAGCCTCGTGGAGTTCAT
CCGGGAGCCGTTGGTCTTCTACAAGGAGGGGTACTACATTCGGGAGTTCTTCCTGGAGGTGCTGAAAGGGGCCGGGAGCG
CGCCCAATATCGTCTTCGAGACCAACCTATTCTCTCTGGTGAAGTCGCTGGTGCGAAACGGCACCGGGATCTCCATCTTC
CTCAGGATGGTGGTGGCGGCCGACACCGACCTGGTCGCGGTCCCCTTCGACCCGCCGCTGCACCTGGACCTCCTGATCGC
CTGGAAGAAGGATACCTATCTCTCCCTTGCCAACCGCGCCTTCGTCGACTTCCTGCTGCAGCATGCGGCGGAGGAGTGGC
GCAGCCAGGCGGAAACCGGCTGA

Upstream 100 bases:

>100_bases
CGGACGATCGCTCTCATTGGAACGACTATATCGGTTGCTTCACAATAAGAAAAATATATAATATTTATCGCTTTGATAGC
TAACGCTTATGGAGGATCGG

Downstream 100 bases:

>100_bases
GCGGCTAAAGCGGGTCCAGGATCTCCTTTAATTTGGCGGCGATCTTTTGCGGGGGGAGCACGAAGTCGACGGCGCCTGTG
TCGGCGGCCGCTTTCGGCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDVRQLRYFIEVAKVKNFTKAAEALHIAQPAVSMAVKKLEQELELSLFNRQDKKVSLTAEGEIFLPHAKRILDDLRAAEL
EMAELKGLSKGEVRLGIPPMISAYFFPDIIRDFKKSYPQLHLSVLGEGAWRIQKMIGQGELDMGVVAGGNFPETLEVRRF
LREEIVICVPPDHPFAQRKSVSLVEFIREPLVFYKEGYYIREFFLEVLKGAGSAPNIVFETNLFSLVKSLVRNGTGISIF
LRMVVAADTDLVAVPFDPPLHLDLLIAWKKDTYLSLANRAFVDFLLQHAAEEWRSQAETG

Sequences:

>Translated_300_residues
MDVRQLRYFIEVAKVKNFTKAAEALHIAQPAVSMAVKKLEQELELSLFNRQDKKVSLTAEGEIFLPHAKRILDDLRAAEL
EMAELKGLSKGEVRLGIPPMISAYFFPDIIRDFKKSYPQLHLSVLGEGAWRIQKMIGQGELDMGVVAGGNFPETLEVRRF
LREEIVICVPPDHPFAQRKSVSLVEFIREPLVFYKEGYYIREFFLEVLKGAGSAPNIVFETNLFSLVKSLVRNGTGISIF
LRMVVAADTDLVAVPFDPPLHLDLLIAWKKDTYLSLANRAFVDFLLQHAAEEWRSQAETG
>Mature_300_residues
MDVRQLRYFIEVAKVKNFTKAAEALHIAQPAVSMAVKKLEQELELSLFNRQDKKVSLTAEGEIFLPHAKRILDDLRAAEL
EMAELKGLSKGEVRLGIPPMISAYFFPDIIRDFKKSYPQLHLSVLGEGAWRIQKMIGQGELDMGVVAGGNFPETLEVRRF
LREEIVICVPPDHPFAQRKSVSLVEFIREPLVFYKEGYYIREFFLEVLKGAGSAPNIVFETNLFSLVKSLVRNGTGISIF
LRMVVAADTDLVAVPFDPPLHLDLLIAWKKDTYLSLANRAFVDFLLQHAAEEWRSQAETG

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=30.8219178082192, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787879, Length=303, Percent_Identity=29.7029702970297, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787530, Length=280, Percent_Identity=28.9285714285714, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=25.2032520325203, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=25.2595155709343, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788887, Length=280, Percent_Identity=28.2142857142857, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787601, Length=232, Percent_Identity=28.448275862069, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=26.9896193771626, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=28.0276816608997, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87081904, Length=232, Percent_Identity=29.3103448275862, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788296, Length=243, Percent_Identity=25.5144032921811, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=23.9669421487603, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788748, Length=285, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788297, Length=176, Percent_Identity=29.5454545454545, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367136, Length=240, Percent_Identity=24.5833333333333, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082132, Length=266, Percent_Identity=24.812030075188, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=24.390243902439, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI48994962, Length=84, Percent_Identity=39.2857142857143, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=23.5059760956175, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=167, Percent_Identity=25.748502994012, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081978, Length=172, Percent_Identity=27.906976744186, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789173, Length=172, Percent_Identity=29.0697674418605, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789506, Length=293, Percent_Identity=21.160409556314, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33965; Mature: 33965

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRQLRYFIEVAKVKNFTKAAEALHIAQPAVSMAVKKLEQELELSLFNRQDKKVSLTAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GEIFLPHAKRILDDLRAAELEMAELKGLSKGEVRLGIPPMISAYFFPDIIRDFKKSYPQL
CCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCHHE
HLSVLGEGAWRIQKMIGQGELDMGVVAGGNFPETLEVRRFLREEIVICVPPDHPFAQRKS
EHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCHHHHCC
VSLVEFIREPLVFYKEGYYIREFFLEVLKGAGSAPNIVFETNLFSLVKSLVRNGTGISIF
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCHHHH
LRMVVAADTDLVAVPFDPPLHLDLLIAWKKDTYLSLANRAFVDFLLQHAAEEWRSQAETG
HHHHHHCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVRQLRYFIEVAKVKNFTKAAEALHIAQPAVSMAVKKLEQELELSLFNRQDKKVSLTAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GEIFLPHAKRILDDLRAAELEMAELKGLSKGEVRLGIPPMISAYFFPDIIRDFKKSYPQL
CCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCHHE
HLSVLGEGAWRIQKMIGQGELDMGVVAGGNFPETLEVRRFLREEIVICVPPDHPFAQRKS
EHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCEEEEECCCCCHHHHCC
VSLVEFIREPLVFYKEGYYIREFFLEVLKGAGSAPNIVFETNLFSLVKSLVRNGTGISIF
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCHHHH
LRMVVAADTDLVAVPFDPPLHLDLLIAWKKDTYLSLANRAFVDFLLQHAAEEWRSQAETG
HHHHHHCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]