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The map label for this gene is mdeA [H]

Identifier: 197117405

GI number: 197117405

Start: 1173527

End: 1174810

Strand: Direct

Name: mdeA [H]

Synonym: Gbem_1015

Alternate gene names: 197117405

Gene position: 1173527-1174810 (Clockwise)

Preceding gene: 308535211

Following gene: 197117406

Centisome position: 25.43

GC content: 56.46

Gene sequence:

>1284_bases
ATGTCAGAGAATTCATTCGGATTCGATACCCTCGCACTTCATGCCGGCCAGACTGTAGATCCTGCTACCCTGTCCCGTGC
GGTTCCCATTTACCAGACATCCTCATATGTCTTCAAGAACTCCGAACACGCCGCCAACCTTTTCGGGCTCAAAGAGTTCG
GCAATATCTACACCCGTCTGATGAACCCCACCACCGACGTCCTTGAGAAGAGGGTGGCGGAATTGGATGGCGGTGTTGCA
GCCCTCGCAGTGGCTTCCGGTCAGGCAGCCACGACCTATGCAGTGTTGAACATCGCCAGCGCCGGGCAGAACATCATATC
CACCAGCTATCTCTATGGCGGTACCTACAACCTGTTCCACTACACTCTGCCGAAACTGGGCATCACCGTGAAGTTCGTCG
ACTCCTCCGACCCGGAGAACATCCGCAAAGCCATCGATGAGAACACTCGTTTGGTGTACAGCGAGGCCATAGGCAACCCC
AAGAATAACGTTGACGACTTCGAGGCCATCGCCAAGGTCGCACACGATGCGGGCATCCCCTATATCGTAGACAACACCGC
GGCTACGCCGTTCGTATTCAAACCTCTCGAGCATGGCGCCGATATCGTCGTTTACTCGCTGACCAAATTCCTGGGAGGCC
ACGGTACCAGCATCGGCGGTTGCGTAGTCGACGGCGGCACCTTCCCGTGGGACAACGGCAAGTTTCCCGAGTTCACCGAG
CCGGATCCCTCCTACCACGGCTTGAAGTTCTGGGACGCGTTAGGCAACATCTCCTACATCATCAAGATGAGGGTAGAACT
TCTGCGCGACATGGGTGCCTGCATTTCACCCTTCAACGCTTTCCAGATCATCCAGGGGATAGAGACCCTGCATGTCAGGA
TGCAGCGTCACGTAGAGAACGCGCAAAAAGTTGCCGAATGGCTGGAGCAGAATCCGCTGGTGAGCTGGGTCAACTATCCT
GGTCTGCCGAGCCACAAAGACCACGCGAACGCCAAGAAGTACTTGAACGGCGCAGGAGCCATCATCGGGTTCGGCATCAA
GGGTGGGCTTGAAGCAGGCATGAAGTTCATCGACAACGTAAAGCTGCTGTCCCACCTGGCCAACATCGGCGATGCCAAGA
GCCTCGTGATCCACCCGGCGTCCACCACTCACCAGCAACTCTCCGCAGAAGAGCAGTTGGCAAGCGGCGTGAGCCCCGAC
TTCATCAGGCTCTCCATAGGCATAGAAGACGTAAAGGACATCATAGCCGACATAGAGCAGGCCCTGAAAGCGGCGCAAGC
CTAG

Upstream 100 bases:

>100_bases
CGGTAGTGAAATTTGTTGACAGCGCGGCTGCAGTTTTTGTATAGAGAATACACCACGTATTCTTTATCAAAGAGCTTCAG
AAAAGGACGGGAGAGAACCT

Downstream 100 bases:

>100_bases
GCGCAAGAGTTAGACCAGTAATAGGTGGGGGGGCGATGCGCAAAGCATCGCCCCTTCTGCGTGTACTGAGCCTGTCCATA
AAGTCCACCCAGTCCACCCC

Product: O-acetyl-L-homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSENSFGFDTLALHAGQTVDPATLSRAVPIYQTSSYVFKNSEHAANLFGLKEFGNIYTRLMNPTTDVLEKRVAELDGGVA
ALAVASGQAATTYAVLNIASAGQNIISTSYLYGGTYNLFHYTLPKLGITVKFVDSSDPENIRKAIDENTRLVYSEAIGNP
KNNVDDFEAIAKVAHDAGIPYIVDNTAATPFVFKPLEHGADIVVYSLTKFLGGHGTSIGGCVVDGGTFPWDNGKFPEFTE
PDPSYHGLKFWDALGNISYIIKMRVELLRDMGACISPFNAFQIIQGIETLHVRMQRHVENAQKVAEWLEQNPLVSWVNYP
GLPSHKDHANAKKYLNGAGAIIGFGIKGGLEAGMKFIDNVKLLSHLANIGDAKSLVIHPASTTHQQLSAEEQLASGVSPD
FIRLSIGIEDVKDIIADIEQALKAAQA

Sequences:

>Translated_427_residues
MSENSFGFDTLALHAGQTVDPATLSRAVPIYQTSSYVFKNSEHAANLFGLKEFGNIYTRLMNPTTDVLEKRVAELDGGVA
ALAVASGQAATTYAVLNIASAGQNIISTSYLYGGTYNLFHYTLPKLGITVKFVDSSDPENIRKAIDENTRLVYSEAIGNP
KNNVDDFEAIAKVAHDAGIPYIVDNTAATPFVFKPLEHGADIVVYSLTKFLGGHGTSIGGCVVDGGTFPWDNGKFPEFTE
PDPSYHGLKFWDALGNISYIIKMRVELLRDMGACISPFNAFQIIQGIETLHVRMQRHVENAQKVAEWLEQNPLVSWVNYP
GLPSHKDHANAKKYLNGAGAIIGFGIKGGLEAGMKFIDNVKLLSHLANIGDAKSLVIHPASTTHQQLSAEEQLASGVSPD
FIRLSIGIEDVKDIIADIEQALKAAQA
>Mature_426_residues
SENSFGFDTLALHAGQTVDPATLSRAVPIYQTSSYVFKNSEHAANLFGLKEFGNIYTRLMNPTTDVLEKRVAELDGGVAA
LAVASGQAATTYAVLNIASAGQNIISTSYLYGGTYNLFHYTLPKLGITVKFVDSSDPENIRKAIDENTRLVYSEAIGNPK
NNVDDFEAIAKVAHDAGIPYIVDNTAATPFVFKPLEHGADIVVYSLTKFLGGHGTSIGGCVVDGGTFPWDNGKFPEFTEP
DPSYHGLKFWDALGNISYIIKMRVELLRDMGACISPFNAFQIIQGIETLHVRMQRHVENAQKVAEWLEQNPLVSWVNYPG
LPSHKDHANAKKYLNGAGAIIGFGIKGGLEAGMKFIDNVKLLSHLANIGDAKSLVIHPASTTHQQLSAEEQLASGVSPDF
IRLSIGIEDVKDIIADIEQALKAAQA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=420, Percent_Identity=35.9523809523809, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=32.541567695962, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI25453487, Length=419, Percent_Identity=32.2195704057279, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1790375, Length=417, Percent_Identity=29.7362110311751, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789383, Length=419, Percent_Identity=28.4009546539379, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71980708, Length=406, Percent_Identity=32.7586206896552, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=422, Percent_Identity=34.1232227488152, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=34.1232227488152, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=53.8990825688073, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=35.1162790697674, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=25.7918552036199, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=366, Percent_Identity=26.5027322404372, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=376, Percent_Identity=25, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=35.377358490566, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46227; Mature: 46096

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSFGFDTLALHAGQTVDPATLSRAVPIYQTSSYVFKNSEHAANLFGLKEFGNIYTRL
CCCCCCCCHHHEECCCCCCCHHHHHCCCCEEECCCCEEECCCCHHHHHHHHHHHHHHHHH
MNPTTDVLEKRVAELDGGVAALAVASGQAATTYAVLNIASAGQNIISTSYLYGGTYNLFH
CCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHEEHHHCCCHHHHHHEEECCCCHHHH
YTLPKLGITVKFVDSSDPENIRKAIDENTRLVYSEAIGNPKNNVDDFEAIAKVAHDAGIP
EECCCCCEEEEEECCCCHHHHHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCC
YIVDNTAATPFVFKPLEHGADIVVYSLTKFLGGHGTSIGGCVVDGGTFPWDNGKFPEFTE
EEECCCCCCCEEECHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCC
PDPSYHGLKFWDALGNISYIIKMRVELLRDMGACISPFNAFQIIQGIETLHVRMQRHVEN
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQKVAEWLEQNPLVSWVNYPGLPSHKDHANAKKYLNGAGAIIGFGIKGGLEAGMKFIDNV
HHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KLLSHLANIGDAKSLVIHPASTTHQQLSAEEQLASGVSPDFIRLSIGIEDVKDIIADIEQ
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHH
ALKAAQA
HHHHHCC
>Mature Secondary Structure 
SENSFGFDTLALHAGQTVDPATLSRAVPIYQTSSYVFKNSEHAANLFGLKEFGNIYTRL
CCCCCCCHHHEECCCCCCCHHHHHCCCCEEECCCCEEECCCCHHHHHHHHHHHHHHHHH
MNPTTDVLEKRVAELDGGVAALAVASGQAATTYAVLNIASAGQNIISTSYLYGGTYNLFH
CCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHEEHHHCCCHHHHHHEEECCCCHHHH
YTLPKLGITVKFVDSSDPENIRKAIDENTRLVYSEAIGNPKNNVDDFEAIAKVAHDAGIP
EECCCCCEEEEEECCCCHHHHHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCC
YIVDNTAATPFVFKPLEHGADIVVYSLTKFLGGHGTSIGGCVVDGGTFPWDNGKFPEFTE
EEECCCCCCCEEECHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCC
PDPSYHGLKFWDALGNISYIIKMRVELLRDMGACISPFNAFQIIQGIETLHVRMQRHVEN
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AQKVAEWLEQNPLVSWVNYPGLPSHKDHANAKKYLNGAGAIIGFGIKGGLEAGMKFIDNV
HHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KLLSHLANIGDAKSLVIHPASTTHQQLSAEEQLASGVSPDFIRLSIGIEDVKDIIADIEQ
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHH
ALKAAQA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]