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The map label for this gene is bioD [C]

Identifier: 197117372

GI number: 197117372

Start: 1139968

End: 1141062

Strand: Reverse

Name: bioD [C]

Synonym: Gbem_0982

Alternate gene names: 197117372

Gene position: 1141062-1139968 (Counterclockwise)

Preceding gene: 197117378

Following gene: 197117367

Centisome position: 24.72

GC content: 59.18

Gene sequence:

>1095_bases
ATGTGCAAGAAGATATTCATAGCGGCGACCGGGCAGCACTGCGGCAAGACCACCATCAGCCTGTCCTTGCTGCACCTGGC
CAAGAAGAAGTACGCCCGCGTCGGATTTATAAAGCCCATAGGGCCCAAGGTTTTCATGCTGGACGGGGTCGAGATGGACA
TGGACGCTGCGCTGATCGCCTCTGTCTACGGGATGGAAGCTGACCGTGCGCTGATGTCCCCCGTGGTGCTCGGCAAGGGA
TACACCAAAAGGTACCTCTCCGGCGAGATCAACCCCGAGGCGCCGCTGGAAAGCATCAAGAAGGCGTGCCGCGAGCTTGA
GGCCAAAAACGATTTCCTGATCATCGAAGGTTCAGGTCACGGCGGGGTCGGCTCCGTGGTCGGCGTCAACAACGCACAGG
TCGCCCGCACCCTGGATGCACCGGTCATCATGGTGGCAAGCGGCGGGATAGGATGCGTCATCGACTCGGTGCAGTTGAAC
CTTTCGCTGTTCCAGCAGCAGGGAGCGGACGTCAAACTGGTCCTGGTGAACAAGCTCCTGCCGAACAAGCGTGAGGAAAC
GCTGGGCTTTCTGCGCAGTTTCTTCGAGAGCCGGCAACAGCAGGTAGCCGGAGCGTTCGACTACTCCCGCACCCTGGCGA
ACCCGACCCTGCTCGACCTTTCCAAGCTGCTGAAGCTGCCCCTTCAAGGGGACCAGCAGGGAAAGAACCGCATCATCCAC
AACATCCAGTTAGGCGCAGCATCCTCCCAGCGTGTCATCGACACCTTGCAGGATTCGACGCTTCTCATCACCACCAGTTC
CCGCGACGAATTGATAGTTACCACATCGGCACTCTACAATATCCCCGCCTACCGGCAGAAGCTGGCCGGCCTGGTGATCC
CCGGCATCGCCCCCATCTCCGCCATCAGCCAAAAGATTCTGGACGACAGCCATATCCCCTACATCAGGACGCTGCAGAAC
ACCGCCGAGGTCTTCATAACCCTCTCCGAGCACGTCTCCAAGATGACCGCTGACGACGATGAGAAGATCAACCTGATCAA
GGCGACCGCCGAAAGCGTCTTCGACTTCGACGCCATCGACGCCATGAGCCGTTAA

Upstream 100 bases:

>100_bases
GCCCGGTACCGTATACCATGTTGCCCCCATTTTGCCAAAACAGTATAATGCCGGTAGTCGAATTGTTATCAGCTGAGAGG
ATGTGACGAGGCTTTAGCAA

Downstream 100 bases:

>100_bases
CAAAAAAAGCCGGCGGAGGGTTCAATCCCCCCGCCGGCTTCCCGCTTCAATCCTGCCGCAAACCTTCTTCGCTACTCCCG
CATGTCAGCTAAAGCGTCAT

Product: BioD/DRTGG domain-containing protein

Products: ADP; Dethiobiotin; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MCKKIFIAATGQHCGKTTISLSLLHLAKKKYARVGFIKPIGPKVFMLDGVEMDMDAALIASVYGMEADRALMSPVVLGKG
YTKRYLSGEINPEAPLESIKKACRELEAKNDFLIIEGSGHGGVGSVVGVNNAQVARTLDAPVIMVASGGIGCVIDSVQLN
LSLFQQQGADVKLVLVNKLLPNKREETLGFLRSFFESRQQQVAGAFDYSRTLANPTLLDLSKLLKLPLQGDQQGKNRIIH
NIQLGAASSQRVIDTLQDSTLLITTSSRDELIVTTSALYNIPAYRQKLAGLVIPGIAPISAISQKILDDSHIPYIRTLQN
TAEVFITLSEHVSKMTADDDEKINLIKATAESVFDFDAIDAMSR

Sequences:

>Translated_364_residues
MCKKIFIAATGQHCGKTTISLSLLHLAKKKYARVGFIKPIGPKVFMLDGVEMDMDAALIASVYGMEADRALMSPVVLGKG
YTKRYLSGEINPEAPLESIKKACRELEAKNDFLIIEGSGHGGVGSVVGVNNAQVARTLDAPVIMVASGGIGCVIDSVQLN
LSLFQQQGADVKLVLVNKLLPNKREETLGFLRSFFESRQQQVAGAFDYSRTLANPTLLDLSKLLKLPLQGDQQGKNRIIH
NIQLGAASSQRVIDTLQDSTLLITTSSRDELIVTTSALYNIPAYRQKLAGLVIPGIAPISAISQKILDDSHIPYIRTLQN
TAEVFITLSEHVSKMTADDDEKINLIKATAESVFDFDAIDAMSR
>Mature_364_residues
MCKKIFIAATGQHCGKTTISLSLLHLAKKKYARVGFIKPIGPKVFMLDGVEMDMDAALIASVYGMEADRALMSPVVLGKG
YTKRYLSGEINPEAPLESIKKACRELEAKNDFLIIEGSGHGGVGSVVGVNNAQVARTLDAPVIMVASGGIGCVIDSVQLN
LSLFQQQGADVKLVLVNKLLPNKREETLGFLRSFFESRQQQVAGAFDYSRTLANPTLLDLSKLLKLPLQGDQQGKNRIIH
NIQLGAASSQRVIDTLQDSTLLITTSSRDELIVTTSALYNIPAYRQKLAGLVIPGIAPISAISQKILDDSHIPYIRTLQN
TAEVFITLSEHVSKMTADDDEKINLIKATAESVFDFDAIDAMSR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0857

COG function: function code R; BioD-like N-terminal domain of phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To Synechocystis PCC 6803 slr0039 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010766 [H]

Pfam domain/function: PF07085 DRTGG [H]

EC number: 6.3.3.3 [C]

Molecular weight: Translated: 39502; Mature: 39502

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKKIFIAATGQHCGKTTISLSLLHLAKKKYARVGFIKPIGPKVFMLDGVEMDMDAALIA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHH
SVYGMEADRALMSPVVLGKGYTKRYLSGEINPEAPLESIKKACRELEAKNDFLIIEGSGH
HHHCCCCCHHHHCHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
GGVGSVVGVNNAQVARTLDAPVIMVASGGIGCVIDSVQLNLSLFQQQGADVKLVLVNKLL
CCCCEEEECCCCHHEEECCCCEEEEECCCCEEEEECHHHHHHHHHHCCCCEEEEEEHHHC
PNKREETLGFLRSFFESRQQQVAGAFDYSRTLANPTLLDLSKLLKLPLQGDQQGKNRIIH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEE
NIQLGAASSQRVIDTLQDSTLLITTSSRDELIVTTSALYNIPAYRQKLAGLVIPGIAPIS
EEEECCCCCHHHHHHHCCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHCCEECCCCCHHH
AISQKILDDSHIPYIRTLQNTAEVFITLSEHVSKMTADDDEKINLIKATAESVFDFDAID
HHHHHHHCCCCCCHHHHHHCCHHEEEEHHHHHHHHCCCCCCCEEEEHHHHHHHHCHHHHH
AMSR
HCCC
>Mature Secondary Structure
MCKKIFIAATGQHCGKTTISLSLLHLAKKKYARVGFIKPIGPKVFMLDGVEMDMDAALIA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHH
SVYGMEADRALMSPVVLGKGYTKRYLSGEINPEAPLESIKKACRELEAKNDFLIIEGSGH
HHHCCCCCHHHHCHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
GGVGSVVGVNNAQVARTLDAPVIMVASGGIGCVIDSVQLNLSLFQQQGADVKLVLVNKLL
CCCCEEEECCCCHHEEECCCCEEEEECCCCEEEEECHHHHHHHHHHCCCCEEEEEEHHHC
PNKREETLGFLRSFFESRQQQVAGAFDYSRTLANPTLLDLSKLLKLPLQGDQQGKNRIIH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEE
NIQLGAASSQRVIDTLQDSTLLITTSSRDELIVTTSALYNIPAYRQKLAGLVIPGIAPIS
EEEECCCCCHHHHHHHCCCEEEEEECCCCCEEEEEHHHHCCHHHHHHHCCEECCCCCHHH
AISQKILDDSHIPYIRTLQNTAEVFITLSEHVSKMTADDDEKINLIKATAESVFDFDAID
HHHHHHHCCCCCCHHHHHHCCHHEEEEHHHHHHHHCCCCCCCEEEEHHHHHHHHCHHHHH
AMSR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {MgCl2}} 0.42 {ATP}} 10 {HCO3-}} 0.045 {7,8-diaminononanoate}} [C]

Substrates: ATP; CO2; 7,8-Diaminononanoate [C]

Specific reaction: ATP + CO2 + 7,8-Diaminononanoate <==> ADP + Dethiobiotin + (3) H+ + Phosphate [C]

General reaction: Carboxylation; Heteroatomic ring closure [C]

Inhibitor: Ag+; Cd2+; Co2+; Cu2+; Hg2+; Ni2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]