Click here to switch to the map view.

The map label for this gene is miaA-1

Identifier: 197117369

GI number: 197117369

Start: 1137171

End: 1138091

Strand: Direct

Name: miaA-1

Synonym: Gbem_0979

Alternate gene names: 197117369

Gene position: 1137171-1138091 (Clockwise)

Preceding gene: 197117368

Following gene: 197117370

Centisome position: 24.64

GC content: 57.76

Gene sequence:

>921_bases
TTGCAGGATAAGGAAAAAAAGATCAAGGTCATCGTCCTTGGAGGCCCCACCGGTTCCGGCAAGAGCGACCTCGCGGTGAA
GCTGGCGGAAGAGATCGGCGGAGAAATCGTCAATGCGGACTCGATGCAGGTCTATCGCAGGCTCGATATCGGGACGGCGA
AGCCCTCCGCTGCAGACCTGGCCCGCGTCCCCCACCACCTGATCGATATTCTCGATCCGAATGAGGATTTCACCGCCTCG
GATTTCAGGCGCGAGGCCACCGCAGCCATCGCCGACATCGAGCGCAGAGGGAAAAGGGCCATTGTCGTCGGCGGGACCGG
GCTGTACATTCGCGCGCTGCTATACGGCTTGGTGGACTCTCCCACAGGTGACCCGGAATTGCGCCGGCAGTTCGATGACG
TGCCGGGCGAGGAACTGCTGAGGCGTCTGTCGCTGGTCGACCCGGAGACTGCGGCGCGCCTGCACCCAAACGACAGGGTT
CGCCTGATAAGGGCGCTGGAGGTGTACACGCAGACCGGGCGCCCGGTTTCGGCTTTCCGCTCGGAACATGCCTTCTCCGA
TGTTCACTACCAAGTGCTCAAAATGGCGATCCGGGTGGAGCGGCAGGAACTGTACCGCCGCATAGACCTCAGGGTTGAGA
AAATGCTCGAGGATGGGCTGGTCGAAGAGGTTCGCTTGCTGCTGGCTGCGGGGTACGGGCATGAGTTGAAAGCTTTGCGC
TCAATCGGCTATAAGGAAATAACCGCTTATCTGGCAGGGGAAATGACGCTGGATGAAGCAGTTACCCTCATAAAAAGAGA
CACGAGGCGCTACGCCAAACGGCAGATGACATGGTTCGGCAAGGAAAATGATATTTATTGGCTTGAATATCCCGGAAGTT
TTGCTACTATCCTTGGACATGTGATTGAATTTCTTGCGTAA

Upstream 100 bases:

>100_bases
CACGGCAGGCCGGTGATGCAGACCCTCACGCTCGCCGAAGTAGAGAAGATGTTCAAGAGAATCTAGCGGTGTCGACCGTT
ACGCAGAAGTGAGGCACCTG

Downstream 100 bases:

>100_bases
GGGAGCGGAATCATGCCGAAAACGCCTTTTAACATCCAGGACCAATATCTGAATCAGTCTCGTAAGGAACGCGTAAAGGT
TTTGGTGCAATTAATGTCCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 1; DMAPP:tRNA dimethylallyltransferase 1; DMATase 1; Isopentenyl-diphosphate:tRNA isopentenyltransferase 1; IPP transferase 1; IPPT 1; IPTase 1

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQDKEKKIKVIVLGGPTGSGKSDLAVKLAEEIGGEIVNADSMQVYRRLDIGTAKPSAADLARVPHHLIDILDPNEDFTAS
DFRREATAAIADIERRGKRAIVVGGTGLYIRALLYGLVDSPTGDPELRRQFDDVPGEELLRRLSLVDPETAARLHPNDRV
RLIRALEVYTQTGRPVSAFRSEHAFSDVHYQVLKMAIRVERQELYRRIDLRVEKMLEDGLVEEVRLLLAAGYGHELKALR
SIGYKEITAYLAGEMTLDEAVTLIKRDTRRYAKRQMTWFGKENDIYWLEYPGSFATILGHVIEFLA

Sequences:

>Translated_306_residues
MQDKEKKIKVIVLGGPTGSGKSDLAVKLAEEIGGEIVNADSMQVYRRLDIGTAKPSAADLARVPHHLIDILDPNEDFTAS
DFRREATAAIADIERRGKRAIVVGGTGLYIRALLYGLVDSPTGDPELRRQFDDVPGEELLRRLSLVDPETAARLHPNDRV
RLIRALEVYTQTGRPVSAFRSEHAFSDVHYQVLKMAIRVERQELYRRIDLRVEKMLEDGLVEEVRLLLAAGYGHELKALR
SIGYKEITAYLAGEMTLDEAVTLIKRDTRRYAKRQMTWFGKENDIYWLEYPGSFATILGHVIEFLA
>Mature_306_residues
MQDKEKKIKVIVLGGPTGSGKSDLAVKLAEEIGGEIVNADSMQVYRRLDIGTAKPSAADLARVPHHLIDILDPNEDFTAS
DFRREATAAIADIERRGKRAIVVGGTGLYIRALLYGLVDSPTGDPELRRQFDDVPGEELLRRLSLVDPETAARLHPNDRV
RLIRALEVYTQTGRPVSAFRSEHAFSDVHYQVLKMAIRVERQELYRRIDLRVEKMLEDGLVEEVRLLLAAGYGHELKALR
SIGYKEITAYLAGEMTLDEAVTLIKRDTRRYAKRQMTWFGKENDIYWLEYPGSFATILGHVIEFLA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=316, Percent_Identity=36.3924050632911, Blast_Score=160, Evalue=1e-39,
Organism=Escherichia coli, GI1790613, Length=287, Percent_Identity=42.5087108013937, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=28.6184210526316, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324848, Length=303, Percent_Identity=34.3234323432343, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24649830, Length=333, Percent_Identity=32.7327327327327, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA1_GEOBB (B5EGD5)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137796.1
- ProteinModelPortal:   B5EGD5
- GeneID:   6780952
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0979
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- InterPro:   IPR010488
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT; PF06414 Zeta_toxin

EC number: =2.5.1.75

Molecular weight: Translated: 34532; Mature: 34532

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKEKKIKVIVLGGPTGSGKSDLAVKLAEEIGGEIVNADSMQVYRRLDIGTAKPSAADL
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHCCCCCCCCHHHH
ARVPHHLIDILDPNEDFTASDFRREATAAIADIERRGKRAIVVGGTGLYIRALLYGLVDS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCC
PTGDPELRRQFDDVPGEELLRRLSLVDPETAARLHPNDRVRLIRALEVYTQTGRPVSAFR
CCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
SEHAFSDVHYQVLKMAIRVERQELYRRIDLRVEKMLEDGLVEEVRLLLAAGYGHELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
SIGYKEITAYLAGEMTLDEAVTLIKRDTRRYAKRQMTWFGKENDIYWLEYPGSFATILGH
HCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHH
VIEFLA
HHHHHC
>Mature Secondary Structure
MQDKEKKIKVIVLGGPTGSGKSDLAVKLAEEIGGEIVNADSMQVYRRLDIGTAKPSAADL
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHCCCCCCCCHHHH
ARVPHHLIDILDPNEDFTASDFRREATAAIADIERRGKRAIVVGGTGLYIRALLYGLVDS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCC
PTGDPELRRQFDDVPGEELLRRLSLVDPETAARLHPNDRVRLIRALEVYTQTGRPVSAFR
CCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
SEHAFSDVHYQVLKMAIRVERQELYRRIDLRVEKMLEDGLVEEVRLLLAAGYGHELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
SIGYKEITAYLAGEMTLDEAVTLIKRDTRRYAKRQMTWFGKENDIYWLEYPGSFATILGH
HCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHH
VIEFLA
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA