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The map label for this gene is dacF [H]

Identifier: 197117367

GI number: 197117367

Start: 1134189

End: 1135052

Strand: Reverse

Name: dacF [H]

Synonym: Gbem_0977

Alternate gene names: 197117367

Gene position: 1135052-1134189 (Counterclockwise)

Preceding gene: 197117372

Following gene: 197117366

Centisome position: 24.59

GC content: 55.79

Gene sequence:

>864_bases
ATGAAAAAATTTTCATCTTTAAGGGTGCTGACATTGACTGCGGGGATACTATTTGCTGCCGTCTGTTACGCAGACGCAGC
CTCCTCACCGTTCGTGCATCCCCCCTCTGCCTACCTTATCCAAGTCAACGGACACACCTACAAGGAAAGAAACGCACACA
TTCGCCGCCCTCCTGCAAGTCTCACCAAAATCATGACAGCACTGATCGTGATGGAGAGGTGCAAACTTGATGAAGTGGTA
GTTGTGAGTCGGGGCGCCGCCAGGGAAACAGGGAGCAGAATTGGCCTTAGGCGAGGCGAAAGGCTGACTGTGCGGGATTT
GCTTGCTGCCACGCTCATGGCCTCAGCCAACGACGCCTGCCGCGCACTTGCCGATCATGCCTGTGGCAATCAAAGGGATT
TCGTGGTACAGATGAACGCCCGGGCCCGGGCCCTGCAATTGCAAAACACGCGCTTCACCAACGCCTGCGGCCACGACAAC
GCCGGACTCTATACCACCGCCCACGATCTGGCACTCCTGACCGAGAAGGCGATGCAAAAGCCATACTTTGCAGAACTAGT
CGCCAGGAAAGAGATGCGCATCCGCACACTGAACAGCAAGCGCTCCTACTACCTCAGGAACAAGAACCGCCTGATAGGGC
GCTATCCAGGTGCAATTGGCGTAAAGACAGGCACGACTCCCAACGCCGGGCAGTGTCTGGTTGCAATAGCCGAGCGTGAA
GAGCGCAAAGTGCTGCTGGTTATCATGCACGCCAGAAATCGGTGGCGGGTAGCACCAGCTATGCTCGACGCGGCATTTGC
TGCTGATTCCGCAGATATCGAGGAAAATCCAGGCAACACCATGAAGGCAACGGTAGGAGATTAG

Upstream 100 bases:

>100_bases
CTTAACCGGCGCCTGTTTGTAAATCCAAGATTGGGCTTTCTCTCACACGTCACGCTCATGGGGCCATTGCAGCCATAACA
AAGAATGCTATAACGAAAAG

Downstream 100 bases:

>100_bases
ATGAAGCATTACCGAAAGGGACTCTGGTTCCAGATAAAGTCCAGGCGCGGCTTCGTAAACATCACCGGTGACATTCAGGA
AGCACTGCGGGAAAGCGGGA

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKKFSSLRVLTLTAGILFAAVCYADAASSPFVHPPSAYLIQVNGHTYKERNAHIRRPPASLTKIMTALIVMERCKLDEVV
VVSRGAARETGSRIGLRRGERLTVRDLLAATLMASANDACRALADHACGNQRDFVVQMNARARALQLQNTRFTNACGHDN
AGLYTTAHDLALLTEKAMQKPYFAELVARKEMRIRTLNSKRSYYLRNKNRLIGRYPGAIGVKTGTTPNAGQCLVAIAERE
ERKVLLVIMHARNRWRVAPAMLDAAFAADSADIEENPGNTMKATVGD

Sequences:

>Translated_287_residues
MKKFSSLRVLTLTAGILFAAVCYADAASSPFVHPPSAYLIQVNGHTYKERNAHIRRPPASLTKIMTALIVMERCKLDEVV
VVSRGAARETGSRIGLRRGERLTVRDLLAATLMASANDACRALADHACGNQRDFVVQMNARARALQLQNTRFTNACGHDN
AGLYTTAHDLALLTEKAMQKPYFAELVARKEMRIRTLNSKRSYYLRNKNRLIGRYPGAIGVKTGTTPNAGQCLVAIAERE
ERKVLLVIMHARNRWRVAPAMLDAAFAADSADIEENPGNTMKATVGD
>Mature_287_residues
MKKFSSLRVLTLTAGILFAAVCYADAASSPFVHPPSAYLIQVNGHTYKERNAHIRRPPASLTKIMTALIVMERCKLDEVV
VVSRGAARETGSRIGLRRGERLTVRDLLAATLMASANDACRALADHACGNQRDFVVQMNARARALQLQNTRFTNACGHDN
AGLYTTAHDLALLTEKAMQKPYFAELVARKEMRIRTLNSKRSYYLRNKNRLIGRYPGAIGVKTGTTPNAGQCLVAIAERE
ERKVLLVIMHARNRWRVAPAMLDAAFAADSADIEENPGNTMKATVGD

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=261, Percent_Identity=34.0996168582375, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787062, Length=271, Percent_Identity=35.4243542435424, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786851, Length=237, Percent_Identity=35.0210970464135, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082054, Length=265, Percent_Identity=32.4528301886792, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 31611; Mature: 31611

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS00024 HEMOPEXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFSSLRVLTLTAGILFAAVCYADAASSPFVHPPSAYLIQVNGHTYKERNAHIRRPPAS
CCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEECCCCCCCCCCCHH
LTKIMTALIVMERCKLDEVVVVSRGAARETGSRIGLRRGERLTVRDLLAATLMASANDAC
HHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHH
RALADHACGNQRDFVVQMNARARALQLQNTRFTNACGHDNAGLYTTAHDLALLTEKAMQK
HHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCC
PYFAELVARKEMRIRTLNSKRSYYLRNKNRLIGRYPGAIGVKTGTTPNAGQCLVAIAERE
CHHHHHHHHHHHHEEEECCCCCEEEECCCCEEEECCCEEEECCCCCCCCCCEEEEEECCC
ERKVLLVIMHARNRWRVAPAMLDAAFAADSADIEENPGNTMKATVGD
CCEEEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MKKFSSLRVLTLTAGILFAAVCYADAASSPFVHPPSAYLIQVNGHTYKERNAHIRRPPAS
CCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEECCCCCCCCCCCHH
LTKIMTALIVMERCKLDEVVVVSRGAARETGSRIGLRRGERLTVRDLLAATLMASANDAC
HHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHH
RALADHACGNQRDFVVQMNARARALQLQNTRFTNACGHDNAGLYTTAHDLALLTEKAMQK
HHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCC
PYFAELVARKEMRIRTLNSKRSYYLRNKNRLIGRYPGAIGVKTGTTPNAGQCLVAIAERE
CHHHHHHHHHHHHEEEECCCCCEEEECCCCEEEECCCEEEECCCCCCCCCCEEEEEECCC
ERKVLLVIMHARNRWRVAPAMLDAAFAADSADIEENPGNTMKATVGD
CCEEEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]