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The map label for this gene is tuf-2 [H]

Identifier: 197117321

GI number: 197117321

Start: 1096444

End: 1097634

Strand: Direct

Name: tuf-2 [H]

Synonym: Gbem_0931

Alternate gene names: 197117321

Gene position: 1096444-1097634 (Clockwise)

Preceding gene: 197117320

Following gene: 197117322

Centisome position: 23.76

GC content: 62.05

Gene sequence:

>1191_bases
ATGGCAAAAGCTAAATTTGAAAGAACCAAGCCGCACGTTAACATCGGCACCATCGGCCACGTCGACCACGGCAAGACTAC
CCTGACCGCGGCAATCACCAAGGTACTCGCAGGCAAAGGCCAGGCCGAGTTCAAGGCGTTCGACCAGATCGACAACGCAC
CGGAAGAGCGCGAGCGTGGTATCACCATCGCTACCGCCCACGTCGAGTACGAGACCGACAAGCGTCACTACGCTCACGTC
GACTGCCCGGGCCACGCCGACTACGTAAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCCATCCTGGTTGTTTC
CGCAGCAGACGGCCCGATGCCGCAGACCCGTGAGCACATCCTGCTCGCGCGTCAGGTCGGCGTCCCCTACATCGTCGTGT
TCCTGAACAAGGCCGACATGGTGGACGACGAGGAGCTCCTCGAGCTGGTCGAGCTGGAAGTTCGCGAACTCCTCTCCTCC
TATGACTTCCCGGGCGACGATATCCCCATCATCAAGGGTTCCGCTCTCAAAGGGCTCGAAGGCGACACCGGCGAGCTGGG
CGAGCAGGCCATCATGAAGCTGATGGAAGCTGTCGACACCTACATCCCGGAGCCGGTTCGCGCCATCGACAAGCCGTTCC
TGATGCCGGTCGAGGACGTGTTCTCCATCTCCGGTCGCGGTACCGTCGCAACCGGCCGTGTCGAGCGCGGCATCGTGAAA
GTCGGCGAGGAAGTCGAGGTTGTCGGCATCAAGACCACCACCAAAACCACCGTCACCGGCGTCGAGATGTTCCGTAAGCT
CCTCGACGAAGGTCGCGCAGGCGACAACATCGGCGCGCTGCTGCGTGGCGTGAAGCGTGAGGACATCGAGCGCGGCCAGG
TTCTGGCCAAGCCGGGCTCCATCACCCCGCACACCAAGTTCAAGGCAGAAGCCTACATCCTCTCCAAAGAGGAAGGTGGC
CGTCACACCCCGTTCTTCAACGGCTACCGTCCGCAGTTCTACTTCAGGACCACCGACGTGACCGGCGTGGTCGACCTCGA
GGCAGGCGTCGAGATGGTTATGCCGGGCGACAACGTCTCGGTGACCGTCAACCTGATCACCCCGATCGCCATGGACGAAG
GTCTGCGCTTCGCAATCCGCGAAGGCGGCCGTACCGTCGGCGCCGGTGTCGTAGCCTCCATCATCGAATAA

Upstream 100 bases:

>100_bases
CTTCGATCATTACGAGCCGGTGCCGAAGTCGGTCGCCGAGGAAATAGTAGCGAAGGTCAAAGGCTAATTATTCAATTTCC
CTATCGAGGAGGATTCCGAA

Downstream 100 bases:

>100_bases
GAATAGAAAAAACATACGGTTTTCATCCGGTTTCCGGCGGGCCGCTCCTGCGTCCCGCCGGAAACTTCGGCTGTAAACTG
AAAACTTGTTTCATCACGAG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSS
YDFPGDDIPIIKGSALKGLEGDTGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEVVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSITPHTKFKAEAYILSKEEGG
RHTPFFNGYRPQFYFRTTDVTGVVDLEAGVEMVMPGDNVSVTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSS
YDFPGDDIPIIKGSALKGLEGDTGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
VGEEVEVVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSITPHTKFKAEAYILSKEEGG
RHTPFFNGYRPQFYFRTTDVTGVVDLEAGVEMVMPGDNVSVTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERGITIATAHVEYETDKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSSY
DFPGDDIPIIKGSALKGLEGDTGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVKV
GEEVEVVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGSITPHTKFKAEAYILSKEEGGR
HTPFFNGYRPQFYFRTTDVTGVVDLEAGVEMVMPGDNVSVTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.8832487309645, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI4503475, Length=454, Percent_Identity=30.8370044052863, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=30.3571428571429, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.7865168539326, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=30.7865168539326, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI194097354, Length=438, Percent_Identity=28.0821917808219, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI194018522, Length=438, Percent_Identity=28.0821917808219, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI194018520, Length=438, Percent_Identity=28.0821917808219, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=28.0182232346241, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=28.695652173913, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4503507, Length=274, Percent_Identity=30.2919708029197, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157426893, Length=370, Percent_Identity=24.8648648648649, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=79.4936708860759, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=79.4936708860759, Blast_Score=619, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=30.4232804232804, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=33.7301587301587, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789108, Length=266, Percent_Identity=32.7067669172932, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=34.4827586206897, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.8796992481203, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.7364341085271, Blast_Score=349, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=31.6964285714286, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=31.6964285714286, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=30.2325581395349, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32566629, Length=451, Percent_Identity=27.0509977827051, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=27.4599542334096, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=445, Percent_Identity=27.4157303370787, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=28.7625418060201, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=40.6896551724138, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=291, Percent_Identity=27.4914089347079, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=235, Percent_Identity=29.3617021276596, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=68.0904522613065, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=30.4545454545455, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=30.4545454545455, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=453, Percent_Identity=25.3863134657837, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=337, Percent_Identity=31.7507418397626, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=27.9569892473118, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=30.8641975308642, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=200, Percent_Identity=32, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.7964376590331, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.7964376590331, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=50.5208333333333, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=29.6213808463252, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=29.6213808463252, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=29.6213808463252, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=29.6213808463252, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.6875, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=32.1311475409836, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI45550900, Length=442, Percent_Identity=29.185520361991, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=29.673590504451, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=28.8256227758007, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=28.8256227758007, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28572034, Length=232, Percent_Identity=32.3275862068966, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43027; Mature: 42896

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCC
ITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSSYDFPGDDIPIIKGSALKGLE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GDTGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEVEVVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCCEEECCCC
ITPHTKFKAEAYILSKEEGGRHTPFFNGYRPQFYFRTTDVTGVVDLEAGVEMVMPGDNVS
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEECCCCCE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAGKGQAEFKAFDQIDNAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCC
ITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEVRELLSSYDFPGDDIPIIKGSALKGLE
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
GDTGELGEQAIMKLMEAVDTYIPEPVRAIDKPFLMPVEDVFSISGRGTVATGRVERGIVK
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEVEVVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLAKPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCCEEECCCC
ITPHTKFKAEAYILSKEEGGRHTPFFNGYRPQFYFRTTDVTGVVDLEAGVEMVMPGDNVS
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEECCCCCE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA