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The map label for this gene is murI [H]

Identifier: 197116675

GI number: 197116675

Start: 332212

End: 333048

Strand: Direct

Name: murI [H]

Synonym: Gbem_0275

Alternate gene names: 197116675

Gene position: 332212-333048 (Clockwise)

Preceding gene: 197116674

Following gene: 197116676

Centisome position: 7.2

GC content: 63.92

Gene sequence:

>837_bases
TTGGCTTGGAAAGCAATCGGCATTTTTGATTCCGGCGTCGGCGGACTCACCGTCCTTAAGGAGGTGGTGCGCGCGCTCCC
CCAGGAGGACACCATCTACCTGGGCGACACGGCACGGGTCCCCTATGGGACCAAGTCCCCGGAGACGGTGGTGCGCTACT
CGCGCCAGATCACCCGTTACCTGTTGAGCCGCGACATTAAAGTCCTGGTGGTCGCCTGCAATACCGCATCGGCCGTGGCG
CTCTCGGCTCTGCAGCAGGAGTTCTCCATCCCCATCGTTGGGGTGATCGAGCCCGGGGCGCGCGCCGCAGCCGCCGTCAC
CAAGAGCGGCAAGGTCGGCGTCATCGGCACCACGGCGACGGTCGCCTCCTCAGCCTATACCAAGGCGATCAAGCGGATCA
ATCCGGAGATCGAGGTGGTGAGCCGCGCCTGCCCCCTGTTCGTGCCGCTGGCCGAGGAGGGGTGGGTGGATAACGAGGTG
GCCCGGCTGACCGCCGGCATCTACCTGGAGGACCTCAAGAAGCACGGCGTCGACACGCTGGTGCTTGGGTGCACCCACTA
CCCGATCCTCAGGAAGGTGATCGCCGAGGTGATGGGGCCGGAAGTGACCCTGGTCGACGCTGCGGAGCAGACGGCGCTCA
CTGTGGCCGGGATTCTGGCCGAGCAGGGGCTTTTGCGCCCCAAGGGGGAGCGGGGAAACCACCATTATTACGTGACCGAC
ATCCCTGCAGGTTTCATCAGGATCGGCAACCGCTTCCTGGGTGGGGATTTCGGAGACGTCTACCAGGTGAACCTGGAGCA
GGAACAGCAGGAGGAAGAAGTTGAAGAAACGGACTAA

Upstream 100 bases:

>100_bases
TGAGGCTCCGCAGAAGTCCCCTTTTCAGGTGGGTAGCGTGCAACACCCATCGGTAGCGACAGGCTGTCGCTTAGATTAAG
TAAATGCAGGGAGAATAACT

Downstream 100 bases:

>100_bases
CAGAGCCAAAGGGACCCTGCTCATAGCCTTCCTGGTCTTCGCGGTCGTGGTCGGCTTGCTGGTGTTCAGGAAATACGAGA
CGGCCACGCGGGTGAAGCCG

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MAWKAIGIFDSGVGGLTVLKEVVRALPQEDTIYLGDTARVPYGTKSPETVVRYSRQITRYLLSRDIKVLVVACNTASAVA
LSALQQEFSIPIVGVIEPGARAAAAVTKSGKVGVIGTTATVASSAYTKAIKRINPEIEVVSRACPLFVPLAEEGWVDNEV
ARLTAGIYLEDLKKHGVDTLVLGCTHYPILRKVIAEVMGPEVTLVDAAEQTALTVAGILAEQGLLRPKGERGNHHYYVTD
IPAGFIRIGNRFLGGDFGDVYQVNLEQEQQEEEVEETD

Sequences:

>Translated_278_residues
MAWKAIGIFDSGVGGLTVLKEVVRALPQEDTIYLGDTARVPYGTKSPETVVRYSRQITRYLLSRDIKVLVVACNTASAVA
LSALQQEFSIPIVGVIEPGARAAAAVTKSGKVGVIGTTATVASSAYTKAIKRINPEIEVVSRACPLFVPLAEEGWVDNEV
ARLTAGIYLEDLKKHGVDTLVLGCTHYPILRKVIAEVMGPEVTLVDAAEQTALTVAGILAEQGLLRPKGERGNHHYYVTD
IPAGFIRIGNRFLGGDFGDVYQVNLEQEQQEEEVEETD
>Mature_277_residues
AWKAIGIFDSGVGGLTVLKEVVRALPQEDTIYLGDTARVPYGTKSPETVVRYSRQITRYLLSRDIKVLVVACNTASAVAL
SALQQEFSIPIVGVIEPGARAAAAVTKSGKVGVIGTTATVASSAYTKAIKRINPEIEVVSRACPLFVPLAEEGWVDNEVA
RLTAGIYLEDLKKHGVDTLVLGCTHYPILRKVIAEVMGPEVTLVDAAEQTALTVAGILAEQGLLRPKGERGNHHYYVTDI
PAGFIRIGNRFLGGDFGDVYQVNLEQEQQEEEVEETD

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=226, Percent_Identity=35.3982300884956, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 29994; Mature: 29862

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWKAIGIFDSGVGGLTVLKEVVRALPQEDTIYLGDTARVPYGTKSPETVVRYSRQITRY
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LLSRDIKVLVVACNTASAVALSALQQEFSIPIVGVIEPGARAAAAVTKSGKVGVIGTTAT
HHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCEEEEECHHH
VASSAYTKAIKRINPEIEVVSRACPLFVPLAEEGWVDNEVARLTAGIYLEDLKKHGVDTL
HHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
VLGCTHYPILRKVIAEVMGPEVTLVDAAEQTALTVAGILAEQGLLRPKGERGNHHYYVTD
EECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
IPAGFIRIGNRFLGGDFGDVYQVNLEQEQQEEEVEETD
CCHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
AWKAIGIFDSGVGGLTVLKEVVRALPQEDTIYLGDTARVPYGTKSPETVVRYSRQITRY
CCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LLSRDIKVLVVACNTASAVALSALQQEFSIPIVGVIEPGARAAAAVTKSGKVGVIGTTAT
HHHCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCCEEEEECHHH
VASSAYTKAIKRINPEIEVVSRACPLFVPLAEEGWVDNEVARLTAGIYLEDLKKHGVDTL
HHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
VLGCTHYPILRKVIAEVMGPEVTLVDAAEQTALTVAGILAEQGLLRPKGERGNHHYYVTD
EECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
IPAGFIRIGNRFLGGDFGDVYQVNLEQEQQEEEVEETD
CCHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA