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The map label for this gene is priA [H]

Identifier: 197116585

GI number: 197116585

Start: 215097

End: 217355

Strand: Reverse

Name: priA [H]

Synonym: Gbem_0185

Alternate gene names: 197116585

Gene position: 217355-215097 (Counterclockwise)

Preceding gene: 197116586

Following gene: 197116583

Centisome position: 4.71

GC content: 66.76

Gene sequence:

>2259_bases
ATGCAGACACCGGTTCCGCACATAGTCGAGGTAGCCATCCCGCTCCCCCTGGAGGGAAGCTTCCACTACCTCGTGCCCGC
GCGCCTGTCGCCGCAAGTAGCCTCCGGAAAACGGGTGCTGGTTCCCTTCGGCAGGCGCAAGGTGACCGGGTATCTCCTGG
GAGACACCGACGCCCCCGGCGCGGGAGAGCTGAAGGAGGTCCTCGACATCCTCGACGAGGAGCCGCTTTTCACCGCGTCC
GAGCTCGACTTCTACCGCTGGATCGCCGGGTACTACCTGCACCCTCTGGGGGAGGTGATCAAGATGGCCCTTCCCGCCGG
GATCAACCTGGTGAGCCGCTACCGCTGCGAGCAGGGTGAGGACGGGACCCCGGTGCTGAAGGAGTTTCTTGCCGGCGGCA
GGAACGTGAGGACCGAGCGCTACTTCCTTCCGGTCGCCGACGAGGAGAGGCGCCCGAGAGGAAAGGGGCTGGAGATCCTT
GAATACCTGCTGGAGATAGGTGAATGCCCGGCGGCCGATCTCAGGAAGCGGTTCGGCAACTGCACCCCGCAATTGAACCG
GCTGGTGCAACTGGGCTGCGCCCGTGTCGAGGCGCGCGAGGTCTACCGCGATCCCTTCCGCTCGCAGGAGTACCAGCACG
ACGGCCCGCTGCCGCTGAACCTTGCGCAGTCCGAGTCGCTCACGCGGCTCACGGCCGCGATCGAGGCCAGCGCCTTCTCC
CCGTTCTTGCTGCACGGGGTGACCGGTTCGGGGAAGACCGAGGTGTACCTGCAGAGCATTGCGGTAGCCCTTGCCGCCGG
GCGGAGCGCTCTGGTGATGGTCCCGGAGATAGCGCTCACCCCGCAGCTCGTCGGGCGCTTCAAGAGGCGCTTCGACTGCG
GCATCGCCGTCCTGCACTCCGGGCTTTCCGACGGCGAGCGCTACGACGAGTGGCGCCGCATCCGCCGCGGCGAGGCCTCT
ATCGTCATCGGCGCCCGCTCCGCCCTCTTCGCGCCGCTGACAGAGGTCGGCGTAATCGTGGTGGACGAGGAGCACGAGGG
ATCCTACAAGCAGTCGGAGGGGGTGCGCTACAACGCGCGGGACCTGGCTCTGGTGCGGGGCAAACTGGCTGGGGCCGTGG
TGATCCTCGGCTCGGCGACCCCGCTGGTCACCAGCTACCATGCCGCGATGAACGGAAAGCTCGGCTACCTCCAGCTTCCG
GACCGGGTGCGGGAACTCCCCATGCCGCAGACGGAGATGCTGGACGCCCGCAGGCAGAAGGGAGAAGTGTTCCTGCCGCC
TCTCATCGAGGCCATGGGAGAGAACCTGGACAACGGGGGGCAGACCCTCCTCTTTTTGAACCGCCGCGGCTTTGCCACCT
ACCTCGTCTGCGAGAGCTGCGGCCACGTGCTCCGCTGCCCCAACTGCGAGGTCACCCTCACCTTCCATCGCCTGAAAGGA
AAGCACGTCTGCCATTACTGCGACTTCTCCATGCTCCCCCCGAAGAACTGCCCCCACTGCGGCGACGGCGAGATAACTCT
TCTGGGGCGGGGGACCGAGCGGGTCGAGGAGCAGGTGCAGCAGCTCTTCCCGCACGCCCGTGTCTCCCGGATGGACCGCG
ACACCACCAGGGGAAAAGGGGGGCACGCCCGCGTTTTGAAGGAGCTCGAGGAGGGAGAGGTCGACATCCTGATCGGCACC
CAGATGATCGCCAAGGGGCACGATTTCCACGGCGTCACCCTGGTCGGGGTGCTCTCGGCCGACGCCTCTTTGAACCTGCC
GGATTTCAGGAGCGCCGAGCGCACCTTTCAGCTGGTTACCCAGGTGATGGGGAGGGCGGGGAGGGGAGATAAACCGGGGC
GGGTGCTGGTGCAGACACTCGCGCCCGAGCACTACGCACTCACCCGCGCGGTGAGCCACGACTTCCAGGGGTTCTACCAG
GAGGAGATCGCCTTTCGGGAGGAGGTAGGATATCCCCCCTTCGCCCATCTCGCCTCGCTTACCCTCTCGGCGGTCGCGGC
CTCGCAGGGGGAGCACTCCGCCGACGAGGCCGCTGCGCTGCTGCAGAAGATCAAGCGGGAGTGCCGGTTGCGGGTGGAGG
TGCTCGGGCCGGTGACGGCGCCGCTTGGGAAGGTGCGGGGACGCTTCAGGTGGCAGATCCTGTTGAAGGGGAGGGAGCGG
GCCGATCTGCACCGGCTGCTGTTCCACTTCAGGGGGGGCTTCAGCCACCCCTCTACGGTCAGGCTCACCATCGACGTGGA
CCCGGTGGACATGCTGTAA

Upstream 100 bases:

>100_bases
AAGCCGCCGGCACCAAGCCGCTTCTGCCACGTTTCACCACGGCCGCTCCCCTTCCTCTTCGTCTCTTCAACCCTTATAAC
CAAAGCCCCAACGACACCGC

Downstream 100 bases:

>100_bases
CATGGGTGATGCGTCCGCTACTCTCCCCCTCCCCCGCAGGCGGGGAGGGGGGTTATACCGCCAAAATGAGCAAGCAAAAA
AAAGGGCCTCGCATAACGGA

Product: primosomal protein N', ATP-dependent helicase

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 752; Mature: 752

Protein sequence:

>752_residues
MQTPVPHIVEVAIPLPLEGSFHYLVPARLSPQVASGKRVLVPFGRRKVTGYLLGDTDAPGAGELKEVLDILDEEPLFTAS
ELDFYRWIAGYYLHPLGEVIKMALPAGINLVSRYRCEQGEDGTPVLKEFLAGGRNVRTERYFLPVADEERRPRGKGLEIL
EYLLEIGECPAADLRKRFGNCTPQLNRLVQLGCARVEAREVYRDPFRSQEYQHDGPLPLNLAQSESLTRLTAAIEASAFS
PFLLHGVTGSGKTEVYLQSIAVALAAGRSALVMVPEIALTPQLVGRFKRRFDCGIAVLHSGLSDGERYDEWRRIRRGEAS
IVIGARSALFAPLTEVGVIVVDEEHEGSYKQSEGVRYNARDLALVRGKLAGAVVILGSATPLVTSYHAAMNGKLGYLQLP
DRVRELPMPQTEMLDARRQKGEVFLPPLIEAMGENLDNGGQTLLFLNRRGFATYLVCESCGHVLRCPNCEVTLTFHRLKG
KHVCHYCDFSMLPPKNCPHCGDGEITLLGRGTERVEEQVQQLFPHARVSRMDRDTTRGKGGHARVLKELEEGEVDILIGT
QMIAKGHDFHGVTLVGVLSADASLNLPDFRSAERTFQLVTQVMGRAGRGDKPGRVLVQTLAPEHYALTRAVSHDFQGFYQ
EEIAFREEVGYPPFAHLASLTLSAVAASQGEHSADEAAALLQKIKRECRLRVEVLGPVTAPLGKVRGRFRWQILLKGRER
ADLHRLLFHFRGGFSHPSTVRLTIDVDPVDML

Sequences:

>Translated_752_residues
MQTPVPHIVEVAIPLPLEGSFHYLVPARLSPQVASGKRVLVPFGRRKVTGYLLGDTDAPGAGELKEVLDILDEEPLFTAS
ELDFYRWIAGYYLHPLGEVIKMALPAGINLVSRYRCEQGEDGTPVLKEFLAGGRNVRTERYFLPVADEERRPRGKGLEIL
EYLLEIGECPAADLRKRFGNCTPQLNRLVQLGCARVEAREVYRDPFRSQEYQHDGPLPLNLAQSESLTRLTAAIEASAFS
PFLLHGVTGSGKTEVYLQSIAVALAAGRSALVMVPEIALTPQLVGRFKRRFDCGIAVLHSGLSDGERYDEWRRIRRGEAS
IVIGARSALFAPLTEVGVIVVDEEHEGSYKQSEGVRYNARDLALVRGKLAGAVVILGSATPLVTSYHAAMNGKLGYLQLP
DRVRELPMPQTEMLDARRQKGEVFLPPLIEAMGENLDNGGQTLLFLNRRGFATYLVCESCGHVLRCPNCEVTLTFHRLKG
KHVCHYCDFSMLPPKNCPHCGDGEITLLGRGTERVEEQVQQLFPHARVSRMDRDTTRGKGGHARVLKELEEGEVDILIGT
QMIAKGHDFHGVTLVGVLSADASLNLPDFRSAERTFQLVTQVMGRAGRGDKPGRVLVQTLAPEHYALTRAVSHDFQGFYQ
EEIAFREEVGYPPFAHLASLTLSAVAASQGEHSADEAAALLQKIKRECRLRVEVLGPVTAPLGKVRGRFRWQILLKGRER
ADLHRLLFHFRGGFSHPSTVRLTIDVDPVDML
>Mature_752_residues
MQTPVPHIVEVAIPLPLEGSFHYLVPARLSPQVASGKRVLVPFGRRKVTGYLLGDTDAPGAGELKEVLDILDEEPLFTAS
ELDFYRWIAGYYLHPLGEVIKMALPAGINLVSRYRCEQGEDGTPVLKEFLAGGRNVRTERYFLPVADEERRPRGKGLEIL
EYLLEIGECPAADLRKRFGNCTPQLNRLVQLGCARVEAREVYRDPFRSQEYQHDGPLPLNLAQSESLTRLTAAIEASAFS
PFLLHGVTGSGKTEVYLQSIAVALAAGRSALVMVPEIALTPQLVGRFKRRFDCGIAVLHSGLSDGERYDEWRRIRRGEAS
IVIGARSALFAPLTEVGVIVVDEEHEGSYKQSEGVRYNARDLALVRGKLAGAVVILGSATPLVTSYHAAMNGKLGYLQLP
DRVRELPMPQTEMLDARRQKGEVFLPPLIEAMGENLDNGGQTLLFLNRRGFATYLVCESCGHVLRCPNCEVTLTFHRLKG
KHVCHYCDFSMLPPKNCPHCGDGEITLLGRGTERVEEQVQQLFPHARVSRMDRDTTRGKGGHARVLKELEEGEVDILIGT
QMIAKGHDFHGVTLVGVLSADASLNLPDFRSAERTFQLVTQVMGRAGRGDKPGRVLVQTLAPEHYALTRAVSHDFQGFYQ
EEIAFREEVGYPPFAHLASLTLSAVAASQGEHSADEAAALLQKIKRECRLRVEVLGPVTAPLGKVRGRFRWQILLKGRER
ADLHRLLFHFRGGFSHPSTVRLTIDVDPVDML

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=781, Percent_Identity=38.1562099871959, Blast_Score=457, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 83287; Mature: 83287

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPVPHIVEVAIPLPLEGSFHYLVPARLSPQVASGKRVLVPFGRRKVTGYLLGDTDAPG
CCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCC
AGELKEVLDILDEEPLFTASELDFYRWIAGYYLHPLGEVIKMALPAGINLVSRYRCEQGE
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
DGTPVLKEFLAGGRNVRTERYFLPVADEERRPRGKGLEILEYLLEIGECPAADLRKRFGN
CCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
CTPQLNRLVQLGCARVEAREVYRDPFRSQEYQHDGPLPLNLAQSESLTRLTAAIEASAFS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCC
PFLLHGVTGSGKTEVYLQSIAVALAAGRSALVMVPEIALTPQLVGRFKRRFDCGIAVLHS
CEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHHHHHHHHHHHHC
GLSDGERYDEWRRIRRGEASIVIGARSALFAPLTEVGVIVVDEEHEGSYKQSEGVRYNAR
CCCCCHHHHHHHHHHCCCCEEEEECHHHHHCCHHHCCEEEEECCCCCCCCCCCCCEECCH
DLALVRGKLAGAVVILGSATPLVTSYHAAMNGKLGYLQLPDRVRELPMPQTEMLDARRQK
HHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCEEEECCHHHHHCCCCHHHHHHHHHHC
GEVFLPPLIEAMGENLDNGGQTLLFLNRRGFATYLVCESCGHVLRCPNCEVTLTFHRLKG
CCEECCHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHCCCEEECCCCCEEEEEEECCC
KHVCHYCDFSMLPPKNCPHCGDGEITLLGRGTERVEEQVQQLFPHARVSRMDRDTTRGKG
CEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
GHARVLKELEEGEVDILIGTQMIAKGHDFHGVTLVGVLSADASLNLPDFRSAERTFQLVT
CHHHHHHHHHCCCEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHH
QVMGRAGRGDKPGRVLVQTLAPEHYALTRAVSHDFQGFYQEEIAFREEVGYPPFAHLASL
HHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TLSAVAASQGEHSADEAAALLQKIKRECRLRVEVLGPVTAPLGKVRGRFRWQILLKGRER
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHCHHHHCCCEEEEEEECCCCH
ADLHRLLFHFRGGFSHPSTVRLTIDVDPVDML
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MQTPVPHIVEVAIPLPLEGSFHYLVPARLSPQVASGKRVLVPFGRRKVTGYLLGDTDAPG
CCCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCC
AGELKEVLDILDEEPLFTASELDFYRWIAGYYLHPLGEVIKMALPAGINLVSRYRCEQGE
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
DGTPVLKEFLAGGRNVRTERYFLPVADEERRPRGKGLEILEYLLEIGECPAADLRKRFGN
CCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
CTPQLNRLVQLGCARVEAREVYRDPFRSQEYQHDGPLPLNLAQSESLTRLTAAIEASAFS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCC
PFLLHGVTGSGKTEVYLQSIAVALAAGRSALVMVPEIALTPQLVGRFKRRFDCGIAVLHS
CEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHHHHHHHHHHHHC
GLSDGERYDEWRRIRRGEASIVIGARSALFAPLTEVGVIVVDEEHEGSYKQSEGVRYNAR
CCCCCHHHHHHHHHHCCCCEEEEECHHHHHCCHHHCCEEEEECCCCCCCCCCCCCEECCH
DLALVRGKLAGAVVILGSATPLVTSYHAAMNGKLGYLQLPDRVRELPMPQTEMLDARRQK
HHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCEEEECCHHHHHCCCCHHHHHHHHHHC
GEVFLPPLIEAMGENLDNGGQTLLFLNRRGFATYLVCESCGHVLRCPNCEVTLTFHRLKG
CCEECCHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHCCCEEECCCCCEEEEEEECCC
KHVCHYCDFSMLPPKNCPHCGDGEITLLGRGTERVEEQVQQLFPHARVSRMDRDTTRGKG
CEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCCCCCCCC
GHARVLKELEEGEVDILIGTQMIAKGHDFHGVTLVGVLSADASLNLPDFRSAERTFQLVT
CHHHHHHHHHCCCEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHH
QVMGRAGRGDKPGRVLVQTLAPEHYALTRAVSHDFQGFYQEEIAFREEVGYPPFAHLASL
HHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
TLSAVAASQGEHSADEAAALLQKIKRECRLRVEVLGPVTAPLGKVRGRFRWQILLKGRER
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHCHHHHCCCEEEEEEECCCCH
ADLHRLLFHFRGGFSHPSTVRLTIDVDPVDML
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]