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The map label for this gene is pcnB [H]

Identifier: 197116551

GI number: 197116551

Start: 182199

End: 183644

Strand: Reverse

Name: pcnB [H]

Synonym: Gbem_0151

Alternate gene names: 197116551

Gene position: 183644-182199 (Counterclockwise)

Preceding gene: 308535150

Following gene: 197116548

Centisome position: 3.98

GC content: 62.79

Gene sequence:

>1446_bases
ATGAATACTAATATGGAAAAAGTCATCATCCCTCGCGCCGAGCACAGCGTTTCCCGCTCAGAATTGAGCCCAAATGCGGT
AAAAACGCTTTACAAGCTCAAAGAACAAGGCTTCACCGCCTACCTGGTGGGAGGCGGCGTACGCGACCTGCTGCTGGGGC
GCGAACCCAAAGATTTTGACGTGGTGACCGACGCCACCCCGGCCCAGCTCAAGAAGATGTTCCGCAACTGCCGCCTGATC
GGCCGCCGTTTCCGGCTGGCGCACCTTCATTTTCACGATGAGATCATAGAGGTAGCCACCTTCCGCTCCACCGTCGATCC
TTCCGCCGAAGCCCCTGCCGAGGAAACTGCTGAAGCCCCCTCCGAAGCGGCTCAGGAACTCCCCCAGGAAACCGTCGTTG
AAGCCAAACTCCTGGAGGAAGAGCGCGAGGGGCGTCGCCGTCGCGGCCATCACGGTCCAAGCATGCTGAAGAGCGAAGAC
GGCATGGTGCTCAGGGACAACGTCTTCGGCACGCCGGAGGAGGATGCAGTTCGCCGCGACTTCACCGTCAACGCCCTTTT
CTACAACATCGCCGACTTCTCCATCATCGACCATGTGGGCGGGATGGAAGACCTGAAGCAGGGACTGATTCGGACCATCG
GCGATCCGTTGGTCCGTTTCACCGAGGACCCAGTCCGCATGATCCGGGCCATCCGTTTCGCCTCCATGCTGGGCTTCAAT
ATCGAGGAACGGACCGAAGCCGCCATCGAGGAGCTCTGCGGCACCATCAACAAGGCGACCCCGCCGCGGCTCTACGAAGA
AGTGCTGAAACTCCTCCTGCTGGGAGCCGGCGAGCGGACCTACCAGATGATGCGCCACAGCGGGCTTTTCGCGCCGCTCT
TCCCCCACTTCGACGCCTGGCTGGAGCGGGAATCCGAAGGGTATCCCCATGCCCGGGTGAGCAAGGGGCTGGAATGGGTG
GATGAGTTGATCGGGCAGGACGAGCCGGTTTCCCCTCCACTGCTCATCGCGCTGGTGTTCGGCGAGTACCTCGAGGAGAA
GATTGCCGAGTTCAGGGATGGCGGACTGCCGCCGCAGCAGGCGGCCGATGCCGCCGTGGCCGCTTTTGCCGCCGAACTGA
CCCCGACTGTAGCCGTCCCCAACCGGGTGCTGGTTGCCGTGAGGGACATACTTTCTCTGCAGCTGCGCTTCCAGAAGACT
CCGGGAAGAAACGGGCGCGGCGTGATCGGCAGGCCGTCCTTTTTGGACGCGCTGCAGTACCTGCGCTTCATGGGAAAGCT
CACCCCGCCGAAGAAATCGCTTGCCGACTGGTGGGAGCGCTACGCGGTCCAGCAAGCTGCGGGGCAGCCGACTGCAGCCG
CCGAGGTTCCAGGCGAGGCGGAGAAAGCCCCCGCGAAGAAGCGCAGGAGGCGGAGGCGGAGAAAGCCCGCCCCCATGCCG
AGCTAG

Upstream 100 bases:

>100_bases
TCACCATAGCAAAGGAAGGTGGATTTCCTCATGACCAAAGTCAAGAGAATAAACATTGCACCCTTTGCCTTTGCCGTTTC
TTTATGCTATTTTCCATCGA

Downstream 100 bases:

>100_bases
TGGATCCAAACAAGAACCCCCTCGCGCACCAAGCCCGAGGGGGTTTCGTTGTCAATCGTCAATCGTCAATTGTCAATTGC
TTCTCAGCTTCCCCTGGTGA

Product: nucleic acid-independent polyadenylating polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNTNMEKVIIPRAEHSVSRSELSPNAVKTLYKLKEQGFTAYLVGGGVRDLLLGREPKDFDVVTDATPAQLKKMFRNCRLI
GRRFRLAHLHFHDEIIEVATFRSTVDPSAEAPAEETAEAPSEAAQELPQETVVEAKLLEEEREGRRRRGHHGPSMLKSED
GMVLRDNVFGTPEEDAVRRDFTVNALFYNIADFSIIDHVGGMEDLKQGLIRTIGDPLVRFTEDPVRMIRAIRFASMLGFN
IEERTEAAIEELCGTINKATPPRLYEEVLKLLLLGAGERTYQMMRHSGLFAPLFPHFDAWLERESEGYPHARVSKGLEWV
DELIGQDEPVSPPLLIALVFGEYLEEKIAEFRDGGLPPQQAADAAVAAFAAELTPTVAVPNRVLVAVRDILSLQLRFQKT
PGRNGRGVIGRPSFLDALQYLRFMGKLTPPKKSLADWWERYAVQQAAGQPTAAAEVPGEAEKAPAKKRRRRRRRKPAPMP
S

Sequences:

>Translated_481_residues
MNTNMEKVIIPRAEHSVSRSELSPNAVKTLYKLKEQGFTAYLVGGGVRDLLLGREPKDFDVVTDATPAQLKKMFRNCRLI
GRRFRLAHLHFHDEIIEVATFRSTVDPSAEAPAEETAEAPSEAAQELPQETVVEAKLLEEEREGRRRRGHHGPSMLKSED
GMVLRDNVFGTPEEDAVRRDFTVNALFYNIADFSIIDHVGGMEDLKQGLIRTIGDPLVRFTEDPVRMIRAIRFASMLGFN
IEERTEAAIEELCGTINKATPPRLYEEVLKLLLLGAGERTYQMMRHSGLFAPLFPHFDAWLERESEGYPHARVSKGLEWV
DELIGQDEPVSPPLLIALVFGEYLEEKIAEFRDGGLPPQQAADAAVAAFAAELTPTVAVPNRVLVAVRDILSLQLRFQKT
PGRNGRGVIGRPSFLDALQYLRFMGKLTPPKKSLADWWERYAVQQAAGQPTAAAEVPGEAEKAPAKKRRRRRRRKPAPMP
S
>Mature_481_residues
MNTNMEKVIIPRAEHSVSRSELSPNAVKTLYKLKEQGFTAYLVGGGVRDLLLGREPKDFDVVTDATPAQLKKMFRNCRLI
GRRFRLAHLHFHDEIIEVATFRSTVDPSAEAPAEETAEAPSEAAQELPQETVVEAKLLEEEREGRRRRGHHGPSMLKSED
GMVLRDNVFGTPEEDAVRRDFTVNALFYNIADFSIIDHVGGMEDLKQGLIRTIGDPLVRFTEDPVRMIRAIRFASMLGFN
IEERTEAAIEELCGTINKATPPRLYEEVLKLLLLGAGERTYQMMRHSGLFAPLFPHFDAWLERESEGYPHARVSKGLEWV
DELIGQDEPVSPPLLIALVFGEYLEEKIAEFRDGGLPPQQAADAAVAAFAAELTPTVAVPNRVLVAVRDILSLQLRFQKT
PGRNGRGVIGRPSFLDALQYLRFMGKLTPPKKSLADWWERYAVQQAAGQPTAAAEVPGEAEKAPAKKRRRRRRRKPAPMP
S

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=463, Percent_Identity=39.0928725701944, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1789436, Length=284, Percent_Identity=29.2253521126761, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=76, Percent_Identity=51.3157894736842, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 53918; Mature: 53918

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNMEKVIIPRAEHSVSRSELSPNAVKTLYKLKEQGFTAYLVGGGVRDLLLGREPKDFD
CCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCC
VVTDATPAQLKKMFRNCRLIGRRFRLAHLHFHDEIIEVATFRSTVDPSAEAPAEETAEAP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCH
SEAAQELPQETVVEAKLLEEEREGRRRRGHHGPSMLKSEDGMVLRDNVFGTPEEDAVRRD
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCCCCCHHHHHHC
FTVNALFYNIADFSIIDHVGGMEDLKQGLIRTIGDPLVRFTEDPVRMIRAIRFASMLGFN
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCC
IEERTEAAIEELCGTINKATPPRLYEEVLKLLLLGAGERTYQMMRHSGLFAPLFPHFDAW
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHH
LERESEGYPHARVSKGLEWVDELIGQDEPVSPPLLIALVFGEYLEEKIAEFRDGGLPPQQ
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AADAAVAAFAAELTPTVAVPNRVLVAVRDILSLQLRFQKTPGRNGRGVIGRPSFLDALQY
HHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LRFMGKLTPPKKSLADWWERYAVQQAAGQPTAAAEVPGEAEKAPAKKRRRRRRRKPAPMP
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHCCHHHHHHHHHHCCCCCCC
S
C
>Mature Secondary Structure
MNTNMEKVIIPRAEHSVSRSELSPNAVKTLYKLKEQGFTAYLVGGGVRDLLLGREPKDFD
CCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCC
VVTDATPAQLKKMFRNCRLIGRRFRLAHLHFHDEIIEVATFRSTVDPSAEAPAEETAEAP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCH
SEAAQELPQETVVEAKLLEEEREGRRRRGHHGPSMLKSEDGMVLRDNVFGTPEEDAVRRD
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCCCCCHHHHHHC
FTVNALFYNIADFSIIDHVGGMEDLKQGLIRTIGDPLVRFTEDPVRMIRAIRFASMLGFN
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHCCC
IEERTEAAIEELCGTINKATPPRLYEEVLKLLLLGAGERTYQMMRHSGLFAPLFPHFDAW
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHH
LERESEGYPHARVSKGLEWVDELIGQDEPVSPPLLIALVFGEYLEEKIAEFRDGGLPPQQ
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AADAAVAAFAAELTPTVAVPNRVLVAVRDILSLQLRFQKTPGRNGRGVIGRPSFLDALQY
HHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LRFMGKLTPPKKSLADWWERYAVQQAAGQPTAAAEVPGEAEKAPAKKRRRRRRRKPAPMP
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHCCHHHHHHHHHHCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]