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The map label for this gene is pcrA [H]

Identifier: 197116476

GI number: 197116476

Start: 90435

End: 92633

Strand: Direct

Name: pcrA [H]

Synonym: Gbem_0076

Alternate gene names: 197116476

Gene position: 90435-92633 (Clockwise)

Preceding gene: 197116475

Following gene: 197116477

Centisome position: 1.96

GC content: 64.07

Gene sequence:

>2199_bases
ATGAAACTGCTCCACAATCTGAACCCTCCCCAAAAAGAGGCCGTGCTCCACGGGGAAGGACCGCTTTTGGTCCTCGCCGG
GGCCGGCTCCGGCAAGACCCGCGTCATCGTGCACCGCATCGCCTACCTGATCCACGAACGCGGGGTCCCCGCCTGGCAGA
TCCTGGGCGTGACCTTTACCAACAAGGCAGCGGGCGAGATGCGTGAGCGCCTGGCGCACATGCTGGGTGACGGGGAACTT
CCGCTTATCTCCACCTTCCACTCCACCTGCGCCAGGATCCTCAGAAGCGAGATCCGCCACCTGGGATACGACAGCAACTT
CGCCATCTATGACGACAGGGACTGCGAAAAGCTCCTGAAGGATTGCGCCGCCGAGCTGAACCTGGACGAGAAGAGATACC
CGGCCAAGGCGCTCGCCGGTGCTCTGGACGAGTTCAAGAACCAGGGGCTCGCGCCGGACGACGTCCCCACGGACTCCCCC
TACCAGGCCACGCTGGCCCGGGTCTACCGCTGCTACCAGGAACGCCTCAAGCGCTGCAACGCGGTCGATTTCGGCGACCT
GCTGCTCCTTGCCGTGCAGCTGCTCGAACAGGTCAAAGAGGTGCGGGACAAATACCTGCAGCGCTACCGCTGGATCATGG
TGGACGAATATCAGGACACGAACCCGGTGCAGTACCGCCTGGTGCAGCTTCTGGCCGGGGAGCGGAAGAACCTCTGCGTG
GTCGGGGACGACGACCAGTCCATCTACGGCTGGCGCGGCGCGGATATTCGGAACATCCTGGAATTCGAGAAGGATTTCCC
GGGGGTGAAGGTGGTGAAGCTGGAGCAGAACTACCGCTCCACCAAGACCATCCTGGACGGCGCCTGGAACGTGGTGCAGA
AAAACCGCGGCCGCAAGCCCAAGCGCCTTTGGACCGACAACCCCGACGGCGAGCGCATCGTCTACCGCACCCTCCCCAAC
GAATGGGAGGAGGCCAGGACCGTCTGCCGCGAGACGGAGCGCTTCCTCGACGAGGGGGGGGACCTCTCGGAGGTGGCCGT
CTTCTACCGCACCAACGCGCAGTCCCGCGCCGTCGAGGACGCCATGGTGTCGGCAGGTGTCGCCTACCATATGGTGGGCG
GGGTCCGCTTCTACGCGAGGCTTGAGGTGAAGGACATCCTCGCCTACCTGAAGGTGCTGGACAACCCCTCCGACGACGTG
GCGCTGAAGCGGATCATCAACACGCCGCCGCGCGGCATCGGCAACACGACGGTGCAGCGTCTCTCCGAGTTCGCCGCGGA
AAAGGGGATACCGCTTTACGACGCCATGCTTGAGGGGGCCTTCGGGCCGCTCCTCTCCGTGGCTGCCAAGGGAAAGGTCG
CCGCTTTCGCGCAGGAGATCGACGGCTACAAGGAGAAGGCGGAGCAGATGCCGCTTTCCGAACTCACCTCCACCATCATC
TACGAGTCCGGGTATTTCGCCCGGCTTAAGGCGCTGGGAAGCGACGAGGCCCAGGAGCGGATCGAGAACCTCCAGGAGCT
GGTGACGGCCATGCAGGCCTACGAGAGCGGCCCCGGCGAGAAGGGATTGGCAGACTTCCTGGAGCAGGTGGCGCTGGTAA
GCGACCTGGAGCAGGAGGGTGACGGGAAGAAATCTTCGGCGACGCTGATGACGCTGCACTCCGCGAAGGGGCTGGAGTTC
CCGCTGGTGTTCATCATCGGCATGGAGGAGCGGCTTTTCCCCCACGTGAGGGCCCTGGAAGATCCGGCCCAGATGGAGGA
GGAGCGCAGGCTCTGCTACGTGGGGATGACCCGCGCCAAGAAGCGCCTTTTCCTGCTGAACGTGCGCCGGCGCCACATCT
TCGGCCAGGAGCAGATGAACCCGCCGGCAAGGTTCATAGCCGACATACCGCGAGAGCTCCTCGACAGCGGGGAAAACTGG
CAGGAGCCTGCGCGACACCGCGAGTTCCCCTCCCACAACTTAGCCTCGCTCTTCGAGGAGGAGATGGAGCCCGAAGAGGA
TGACAACGAGGTGCGCATGGTGCCGGAGCACGACGAGGGTATCTACCTCGGCATGAAGGTGCGCCACGCCCAGTTCGGCC
CCGGAACCATCCGCAAAATCGAAGGGGAGGGTGACAACCAGAAGGTGATCGTCTGGTTCAACTCGCTGGGAGGGCCAAAG
AAGCTCCTGGTCCGCTTCGCGGGGCTTGAGCGGGCGTGA

Upstream 100 bases:

>100_bases
TGTTCCGCCTTTAAGGTTTACCTCCCCCTACCGAAGTGCTATAAAAGATTCCGCTTTCGCACCGGCGGCCCCTCCAGCGC
CACCGGTTTCAAGGAACGAC

Downstream 100 bases:

>100_bases
TAACGGTAGTATAGTAGACGCATCATTATAATGAGACTATCCAAGGAGGCCCACCCATGAAAAAACTTTGCGTCCTAATG
ATCTCCATGCTGCTCTTCTC

Product: ATP-dependent DNA helicase UvrD/REP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MKLLHNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAYLIHERGVPAWQILGVTFTNKAAGEMRERLAHMLGDGEL
PLISTFHSTCARILRSEIRHLGYDSNFAIYDDRDCEKLLKDCAAELNLDEKRYPAKALAGALDEFKNQGLAPDDVPTDSP
YQATLARVYRCYQERLKRCNAVDFGDLLLLAVQLLEQVKEVRDKYLQRYRWIMVDEYQDTNPVQYRLVQLLAGERKNLCV
VGDDDQSIYGWRGADIRNILEFEKDFPGVKVVKLEQNYRSTKTILDGAWNVVQKNRGRKPKRLWTDNPDGERIVYRTLPN
EWEEARTVCRETERFLDEGGDLSEVAVFYRTNAQSRAVEDAMVSAGVAYHMVGGVRFYARLEVKDILAYLKVLDNPSDDV
ALKRIINTPPRGIGNTTVQRLSEFAAEKGIPLYDAMLEGAFGPLLSVAAKGKVAAFAQEIDGYKEKAEQMPLSELTSTII
YESGYFARLKALGSDEAQERIENLQELVTAMQAYESGPGEKGLADFLEQVALVSDLEQEGDGKKSSATLMTLHSAKGLEF
PLVFIIGMEERLFPHVRALEDPAQMEEERRLCYVGMTRAKKRLFLLNVRRRHIFGQEQMNPPARFIADIPRELLDSGENW
QEPARHREFPSHNLASLFEEEMEPEEDDNEVRMVPEHDEGIYLGMKVRHAQFGPGTIRKIEGEGDNQKVIVWFNSLGGPK
KLLVRFAGLERA

Sequences:

>Translated_732_residues
MKLLHNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAYLIHERGVPAWQILGVTFTNKAAGEMRERLAHMLGDGEL
PLISTFHSTCARILRSEIRHLGYDSNFAIYDDRDCEKLLKDCAAELNLDEKRYPAKALAGALDEFKNQGLAPDDVPTDSP
YQATLARVYRCYQERLKRCNAVDFGDLLLLAVQLLEQVKEVRDKYLQRYRWIMVDEYQDTNPVQYRLVQLLAGERKNLCV
VGDDDQSIYGWRGADIRNILEFEKDFPGVKVVKLEQNYRSTKTILDGAWNVVQKNRGRKPKRLWTDNPDGERIVYRTLPN
EWEEARTVCRETERFLDEGGDLSEVAVFYRTNAQSRAVEDAMVSAGVAYHMVGGVRFYARLEVKDILAYLKVLDNPSDDV
ALKRIINTPPRGIGNTTVQRLSEFAAEKGIPLYDAMLEGAFGPLLSVAAKGKVAAFAQEIDGYKEKAEQMPLSELTSTII
YESGYFARLKALGSDEAQERIENLQELVTAMQAYESGPGEKGLADFLEQVALVSDLEQEGDGKKSSATLMTLHSAKGLEF
PLVFIIGMEERLFPHVRALEDPAQMEEERRLCYVGMTRAKKRLFLLNVRRRHIFGQEQMNPPARFIADIPRELLDSGENW
QEPARHREFPSHNLASLFEEEMEPEEDDNEVRMVPEHDEGIYLGMKVRHAQFGPGTIRKIEGEGDNQKVIVWFNSLGGPK
KLLVRFAGLERA
>Mature_732_residues
MKLLHNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAYLIHERGVPAWQILGVTFTNKAAGEMRERLAHMLGDGEL
PLISTFHSTCARILRSEIRHLGYDSNFAIYDDRDCEKLLKDCAAELNLDEKRYPAKALAGALDEFKNQGLAPDDVPTDSP
YQATLARVYRCYQERLKRCNAVDFGDLLLLAVQLLEQVKEVRDKYLQRYRWIMVDEYQDTNPVQYRLVQLLAGERKNLCV
VGDDDQSIYGWRGADIRNILEFEKDFPGVKVVKLEQNYRSTKTILDGAWNVVQKNRGRKPKRLWTDNPDGERIVYRTLPN
EWEEARTVCRETERFLDEGGDLSEVAVFYRTNAQSRAVEDAMVSAGVAYHMVGGVRFYARLEVKDILAYLKVLDNPSDDV
ALKRIINTPPRGIGNTTVQRLSEFAAEKGIPLYDAMLEGAFGPLLSVAAKGKVAAFAQEIDGYKEKAEQMPLSELTSTII
YESGYFARLKALGSDEAQERIENLQELVTAMQAYESGPGEKGLADFLEQVALVSDLEQEGDGKKSSATLMTLHSAKGLEF
PLVFIIGMEERLFPHVRALEDPAQMEEERRLCYVGMTRAKKRLFLLNVRRRHIFGQEQMNPPARFIADIPRELLDSGENW
QEPARHREFPSHNLASLFEEEMEPEEDDNEVRMVPEHDEGIYLGMKVRHAQFGPGTIRKIEGEGDNQKVIVWFNSLGGPK
KLLVRFAGLERA

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=733, Percent_Identity=40.5184174624829, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI48994965, Length=640, Percent_Identity=38.4375, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=337, Percent_Identity=28.1899109792285, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=741, Percent_Identity=28.3400809716599, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324477, Length=703, Percent_Identity=23.3285917496444, Blast_Score=121, Evalue=4e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82899; Mature: 82899

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLHNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAYLIHERGVPAWQILGVTFT
CCCCCCCCCCHHHCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEC
NKAAGEMRERLAHMLGDGELPLISTFHSTCARILRSEIRHLGYDSNFAIYDDRDCEKLLK
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
DCAAELNLDEKRYPAKALAGALDEFKNQGLAPDDVPTDSPYQATLARVYRCYQERLKRCN
HHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
AVDFGDLLLLAVQLLEQVKEVRDKYLQRYRWIMVDEYQDTNPVQYRLVQLLAGERKNLCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCEEE
VGDDDQSIYGWRGADIRNILEFEKDFPGVKVVKLEQNYRSTKTILDGAWNVVQKNRGRKP
ECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
KRLWTDNPDGERIVYRTLPNEWEEARTVCRETERFLDEGGDLSEVAVFYRTNAQSRAVED
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHH
AMVSAGVAYHMVGGVRFYARLEVKDILAYLKVLDNPSDDVALKRIINTPPRGIGNTTVQR
HHHHCCHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHH
LSEFAAEKGIPLYDAMLEGAFGPLLSVAAKGKVAAFAQEIDGYKEKAEQMPLSELTSTII
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YESGYFARLKALGSDEAQERIENLQELVTAMQAYESGPGEKGLADFLEQVALVSDLEQEG
HCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
DGKKSSATLMTLHSAKGLEFPLVFIIGMEERLFPHVRALEDPAQMEEERRLCYVGMTRAK
CCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHCCEEEEEHHHHH
KRLFLLNVRRRHIFGQEQMNPPARFIADIPRELLDSGENWQEPARHREFPSHNLASLFEE
HHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHH
EMEPEEDDNEVRMVPEHDEGIYLGMKVRHAQFGPGTIRKIEGEGDNQKVIVWFNSLGGPK
HCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCEEEEEECCCCCHH
KLLVRFAGLERA
HHHHHHHCCCCC
>Mature Secondary Structure
MKLLHNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAYLIHERGVPAWQILGVTFT
CCCCCCCCCCHHHCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEC
NKAAGEMRERLAHMLGDGELPLISTFHSTCARILRSEIRHLGYDSNFAIYDDRDCEKLLK
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
DCAAELNLDEKRYPAKALAGALDEFKNQGLAPDDVPTDSPYQATLARVYRCYQERLKRCN
HHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
AVDFGDLLLLAVQLLEQVKEVRDKYLQRYRWIMVDEYQDTNPVQYRLVQLLAGERKNLCV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCEEE
VGDDDQSIYGWRGADIRNILEFEKDFPGVKVVKLEQNYRSTKTILDGAWNVVQKNRGRKP
ECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
KRLWTDNPDGERIVYRTLPNEWEEARTVCRETERFLDEGGDLSEVAVFYRTNAQSRAVED
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEEECCCHHHHHHH
AMVSAGVAYHMVGGVRFYARLEVKDILAYLKVLDNPSDDVALKRIINTPPRGIGNTTVQR
HHHHCCHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHH
LSEFAAEKGIPLYDAMLEGAFGPLLSVAAKGKVAAFAQEIDGYKEKAEQMPLSELTSTII
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YESGYFARLKALGSDEAQERIENLQELVTAMQAYESGPGEKGLADFLEQVALVSDLEQEG
HCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
DGKKSSATLMTLHSAKGLEFPLVFIIGMEERLFPHVRALEDPAQMEEERRLCYVGMTRAK
CCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHCCHHHHHHHCCEEEEEHHHHH
KRLFLLNVRRRHIFGQEQMNPPARFIADIPRELLDSGENWQEPARHREFPSHNLASLFEE
HHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHH
EMEPEEDDNEVRMVPEHDEGIYLGMKVRHAQFGPGTIRKIEGEGDNQKVIVWFNSLGGPK
HCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCEEEEEECCCCCHH
KLLVRFAGLERA
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA