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The map label for this gene is cooF [H]

Identifier: 197116471

GI number: 197116471

Start: 85657

End: 86226

Strand: Direct

Name: cooF [H]

Synonym: Gbem_0071

Alternate gene names: 197116471

Gene position: 85657-86226 (Clockwise)

Preceding gene: 197116470

Following gene: 197116472

Centisome position: 1.86

GC content: 65.79

Gene sequence:

>570_bases
ATGAAGCAACAGCAGCGTGAGAATGCCATAGTCTTTGCCGATCCCGAGCGCTGTCTCGGCTGCCACGGCTGCGAGCTGGC
CTGCGCCCTGGCCCATTCCGGAACCGGGCTGCACCAGGCGGCGCTTCAGGGGCTGACGCTTCGGCCCCGCAATCGCGTCG
TGGCGGCGGGAGACGTGATGATGCCGGTGCAGTGCCGACAGTGCGAGGATGCCCCCTGCGCCTGCGCCTGCCCCACCGGA
GCCATGCTGCAGGAAAAAGGCGCGGTCGGGGTCCGCGAGAAGAACTGCGTCGGCTGCAAAGTCTGCGTCATGGTCTGCCC
CTTCGGCGCCATCAGCGTGAAACACGAGGGGAGCGAAGAGGCAACCGGACGCACCAACGGCGGCGTGGCCAGAAAGTGCG
ATCTCTGCGCCGGGACGGGGCGGGAGATTCCCGCCTGCGTCGAGGGGTGCCCCACCAAGGCGCTCACCCTGGTCGACCTG
GAAGAGTACCGACAGAGCCTCATCGAAGCCAGGGCCGCCGAACTCGCCCAATCCCTACAATTTCTCAAGAAAAGGAAGGC
CAAACTATGA

Upstream 100 bases:

>100_bases
CGGTTCCAGTGTCCCGACTGCGGAGGAGGGGACGTCGAGCTGTCGGGTGGGAGGGAACTTTATTTGGAGAGCTTGGAGGC
CATATGCAAAGGAGACCACA

Downstream 100 bases:

>100_bases
CCCTCAATCTCACCACCGATCCGTCCGCGGCAACGCTGTTGCCGATCGCCAAGAAAGAAGGGATCTCCACGGTATGGGAC
CGCCACCGCGCCATGCAGCC

Product: carbon monoxide dehydrogenase-associated iron-sulfur cluster-binding oxidoreductase CooF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MKQQQRENAIVFADPERCLGCHGCELACALAHSGTGLHQAALQGLTLRPRNRVVAAGDVMMPVQCRQCEDAPCACACPTG
AMLQEKGAVGVREKNCVGCKVCVMVCPFGAISVKHEGSEEATGRTNGGVARKCDLCAGTGREIPACVEGCPTKALTLVDL
EEYRQSLIEARAAELAQSLQFLKKRKAKL

Sequences:

>Translated_189_residues
MKQQQRENAIVFADPERCLGCHGCELACALAHSGTGLHQAALQGLTLRPRNRVVAAGDVMMPVQCRQCEDAPCACACPTG
AMLQEKGAVGVREKNCVGCKVCVMVCPFGAISVKHEGSEEATGRTNGGVARKCDLCAGTGREIPACVEGCPTKALTLVDL
EEYRQSLIEARAAELAQSLQFLKKRKAKL
>Mature_189_residues
MKQQQRENAIVFADPERCLGCHGCELACALAHSGTGLHQAALQGLTLRPRNRVVAAGDVMMPVQCRQCEDAPCACACPTG
AMLQEKGAVGVREKNCVGCKVCVMVCPFGAISVKHEGSEEATGRTNGGVARKCDLCAGTGREIPACVEGCPTKALTLVDL
EEYRQSLIEARAAELAQSLQFLKKRKAKL

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH). CooF is required in stoichiometric amounts in vitro for anchoring CODH to the membrane as well as

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=169, Percent_Identity=39.6449704142012, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1788811, Length=152, Percent_Identity=42.7631578947368, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87082180, Length=158, Percent_Identity=37.3417721518987, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=41.5094339622642, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI87082179, Length=157, Percent_Identity=38.2165605095541, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789079, Length=198, Percent_Identity=35.3535353535354, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI87082114, Length=189, Percent_Identity=35.4497354497354, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1787872, Length=164, Percent_Identity=34.7560975609756, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787122, Length=190, Percent_Identity=31.0526315789474, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI2367345, Length=155, Percent_Identity=31.6129032258064, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI226510944, Length=165, Percent_Identity=30.3030303030303, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1787749, Length=188, Percent_Identity=29.2553191489362, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1787963, Length=156, Percent_Identity=26.9230769230769, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20088; Mature: 20088

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
11.6 %Cys+Met (Translated Protein)
9.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
11.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQQRENAIVFADPERCLGCHGCELACALAHSGTGLHQAALQGLTLRPRNRVVAAGDVM
CCCCCCCCEEEEECHHHHCCCCCCHHEEEHHCCCCCHHHHHHCCCEECCCCCEEEECCEE
MPVQCRQCEDAPCACACPTGAMLQEKGAVGVREKNCVGCKVCVMVCPFGAISVKHEGSEE
CCCHHCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCCHHHHEEECCCCCCEEEECCCCCC
ATGRTNGGVARKCDLCAGTGREIPACVEGCPTKALTLVDLEEYRQSLIEARAAELAQSLQ
CCCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
FLKKRKAKL
HHHHHHCCC
>Mature Secondary Structure
MKQQQRENAIVFADPERCLGCHGCELACALAHSGTGLHQAALQGLTLRPRNRVVAAGDVM
CCCCCCCCEEEEECHHHHCCCCCCHHEEEHHCCCCCHHHHHHCCCEECCCCCEEEECCEE
MPVQCRQCEDAPCACACPTGAMLQEKGAVGVREKNCVGCKVCVMVCPFGAISVKHEGSEE
CCCHHCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCCHHHHEEECCCCCCEEEECCCCCC
ATGRTNGGVARKCDLCAGTGREIPACVEGCPTKALTLVDLEEYRQSLIEARAAELAQSLQ
CCCCCCCCCCEECCCCCCCCCCCCHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
FLKKRKAKL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1644755 [H]