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The map label for this gene is patB [H]

Identifier: 197116456

GI number: 197116456

Start: 68429

End: 69586

Strand: Direct

Name: patB [H]

Synonym: Gbem_0056

Alternate gene names: 197116456

Gene position: 68429-69586 (Clockwise)

Preceding gene: 197116455

Following gene: 197116457

Centisome position: 1.48

GC content: 65.46

Gene sequence:

>1158_bases
ATGACCAAAAAGTTCGACTTCGACGAGATCATCGACCGGCGCGGCACCTCCAGCGAAAAGTGGGACAAGTACCGCGACCG
CGACATCATTCCCCTCTGGGTGGCGGACATGGATTTCCGCTCCCCCCCCGCCGTCATCGATGCGCTACACGAGCGGGTGA
GCCACGGCGTCTTCGGCTACACCGCCCCTTCCGAGAGCCTCGTCGAGGCGGTGCTCCAGTCGCTTAGGGACGAGTTCGGC
TGGCAGGTGGCGAAGGAGTGGATCGTCTGGCTTCCCGGTCTGGTGACCGGGCTCAATGTCTCCTGCCGGGCGGTAGGTGC
GGCGGGTGACGACGTCATCACCCTCACCCCGGTCTATCCCCCCTTCATGTCCGCGCCCCCTTTGTCCGGGCGAAATGCCA
TAAAGGTGCCGCTTCGATGCGAAGAGGGGCGCTGGGGTTTCAGCCTTGAGGACCTGGAGCGCGCGGTAACCCCCGCCACG
AAGCTACTTTTGCTTTGCAGCCCGCACAACCCGGTCGGGCGCGTCTGGAGCCGCGAGGAGCTGACGCAACTGGCGCAGTT
CGCGCAAAGGCACGACCTGGTGATCTGCAGCGACGAGATCCATGCCGGCCTGGTGCTGGAAGAAGGGGTGCGGCATATCC
CTCTTGCCACCCTCTCCCCCGAGATAAGCGCGCGCACCATCACGCTCCTTGCACCCAGCAAGACTTTCAACGTTCCGGGG
CTCGGCTGTTCGTTCGCCGTCATCTCCGACGACACGCTGCGCCGCGCCTTCAAGAAAGCGATGGGAAGGATCGTCCCCCA
CGTGAACCTCCTGGGATACACGGCTGCCGAGGCCGCTTATCGGCACGGGGAAGAGTGGCGGCAGGAACTGATCGCCTACC
TGCGCGGCAACCGCGACTTGGTCGCGCGGGAAGTGGCGCGGATGCCGGGGCTGACCGTGGCGCCGGTGGAGGCGACCTAT
CTTTCCTGGATCGACCTGCGCCAGACCGGGATCGAGGACCCGGTAACTTTCTTCGAGGAGGCGGGGGTGGGCCTTTCCGG
TGGGGCAGACTTCGGGCTCCCTTGCTACGTGAGGCTCAATTTCGGCTGTCGCCGCGACCTCCTGGTCGAGGCGCTGCGGC
GCATGCGGGTGGCGCTGGAGGCGCGTACGGGAAACTGA

Upstream 100 bases:

>100_bases
TCACGCCGCTCGGTGCTCCGCGCGAGATCCCGTTCGTGATCAGGGAGACGCGCCGCAAGTTCCAGTACAGCATCGCGCAA
CTGACGGTATGGGAGCTTAA

Downstream 100 bases:

>100_bases
CACGACCACACAGGAGATATCGTATGAAAAGGACAGATATATTTAAGATCACACTGGCAGGGATCGCCGCAATCGTGGCG
GTTACCTTTGGAGCGCCACG

Product: pyridoxal phosphate-dependent enzyme

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTKKFDFDEIIDRRGTSSEKWDKYRDRDIIPLWVADMDFRSPPAVIDALHERVSHGVFGYTAPSESLVEAVLQSLRDEFG
WQVAKEWIVWLPGLVTGLNVSCRAVGAAGDDVITLTPVYPPFMSAPPLSGRNAIKVPLRCEEGRWGFSLEDLERAVTPAT
KLLLLCSPHNPVGRVWSREELTQLAQFAQRHDLVICSDEIHAGLVLEEGVRHIPLATLSPEISARTITLLAPSKTFNVPG
LGCSFAVISDDTLRRAFKKAMGRIVPHVNLLGYTAAEAAYRHGEEWRQELIAYLRGNRDLVAREVARMPGLTVAPVEATY
LSWIDLRQTGIEDPVTFFEEAGVGLSGGADFGLPCYVRLNFGCRRDLLVEALRRMRVALEARTGN

Sequences:

>Translated_385_residues
MTKKFDFDEIIDRRGTSSEKWDKYRDRDIIPLWVADMDFRSPPAVIDALHERVSHGVFGYTAPSESLVEAVLQSLRDEFG
WQVAKEWIVWLPGLVTGLNVSCRAVGAAGDDVITLTPVYPPFMSAPPLSGRNAIKVPLRCEEGRWGFSLEDLERAVTPAT
KLLLLCSPHNPVGRVWSREELTQLAQFAQRHDLVICSDEIHAGLVLEEGVRHIPLATLSPEISARTITLLAPSKTFNVPG
LGCSFAVISDDTLRRAFKKAMGRIVPHVNLLGYTAAEAAYRHGEEWRQELIAYLRGNRDLVAREVARMPGLTVAPVEATY
LSWIDLRQTGIEDPVTFFEEAGVGLSGGADFGLPCYVRLNFGCRRDLLVEALRRMRVALEARTGN
>Mature_384_residues
TKKFDFDEIIDRRGTSSEKWDKYRDRDIIPLWVADMDFRSPPAVIDALHERVSHGVFGYTAPSESLVEAVLQSLRDEFGW
QVAKEWIVWLPGLVTGLNVSCRAVGAAGDDVITLTPVYPPFMSAPPLSGRNAIKVPLRCEEGRWGFSLEDLERAVTPATK
LLLLCSPHNPVGRVWSREELTQLAQFAQRHDLVICSDEIHAGLVLEEGVRHIPLATLSPEISARTITLLAPSKTFNVPGL
GCSFAVISDDTLRRAFKKAMGRIVPHVNLLGYTAAEAAYRHGEEWRQELIAYLRGNRDLVAREVARMPGLTVAPVEATYL
SWIDLRQTGIEDPVTFFEEAGVGLSGGADFGLPCYVRLNFGCRRDLLVEALRRMRVALEARTGN

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56713254, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI56713256, Length=213, Percent_Identity=29.1079812206573, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI95147551, Length=245, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI169881279, Length=245, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4507369, Length=378, Percent_Identity=23.5449735449735, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI169881281, Length=153, Percent_Identity=32.0261437908497, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787909, Length=391, Percent_Identity=31.7135549872123, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788627, Length=214, Percent_Identity=28.9719626168224, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786816, Length=350, Percent_Identity=23.7142857142857, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567663, Length=373, Percent_Identity=24.1286863270777, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17567369, Length=146, Percent_Identity=29.4520547945205, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994476, Length=144, Percent_Identity=29.8611111111111, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994472, Length=144, Percent_Identity=29.8611111111111, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322401, Length=347, Percent_Identity=25.6484149855908, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573069, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646114, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573067, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573065, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 42835; Mature: 42703

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKFDFDEIIDRRGTSSEKWDKYRDRDIIPLWVADMDFRSPPAVIDALHERVSHGVFGY
CCCCCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
TAPSESLVEAVLQSLRDEFGWQVAKEWIVWLPGLVTGLNVSCRAVGAAGDDVITLTPVYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCC
PFMSAPPLSGRNAIKVPLRCEEGRWGFSLEDLERAVTPATKLLLLCSPHNPVGRVWSREE
CCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCHHEEEEEECCCCCHHHCCCHHH
LTQLAQFAQRHDLVICSDEIHAGLVLEEGVRHIPLATLSPEISARTITLLAPSKTFNVPG
HHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCCCEECCCCCCCEEEEEEECCCCCCCCC
LGCSFAVISDDTLRRAFKKAMGRIVPHVNLLGYTAAEAAYRHGEEWRQELIAYLRGNRDL
CCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHH
VAREVARMPGLTVAPVEATYLSWIDLRQTGIEDPVTFFEEAGVGLSGGADFGLPCYVRLN
HHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEC
FGCRRDLLVEALRRMRVALEARTGN
CCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKKFDFDEIIDRRGTSSEKWDKYRDRDIIPLWVADMDFRSPPAVIDALHERVSHGVFGY
CCCCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
TAPSESLVEAVLQSLRDEFGWQVAKEWIVWLPGLVTGLNVSCRAVGAAGDDVITLTPVYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCC
PFMSAPPLSGRNAIKVPLRCEEGRWGFSLEDLERAVTPATKLLLLCSPHNPVGRVWSREE
CCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCHHEEEEEECCCCCHHHCCCHHH
LTQLAQFAQRHDLVICSDEIHAGLVLEEGVRHIPLATLSPEISARTITLLAPSKTFNVPG
HHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCCCEECCCCCCCEEEEEEECCCCCCCCC
LGCSFAVISDDTLRRAFKKAMGRIVPHVNLLGYTAAEAAYRHGEEWRQELIAYLRGNRDL
CCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHH
VAREVARMPGLTVAPVEATYLSWIDLRQTGIEDPVTFFEEAGVGLSGGADFGLPCYVRLN
HHHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEC
FGCRRDLLVEALRRMRVALEARTGN
CCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]