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The map label for this gene is oprF [H]

Identifier: 197116453

GI number: 197116453

Start: 63912

End: 64913

Strand: Direct

Name: oprF [H]

Synonym: Gbem_0053

Alternate gene names: 197116453

Gene position: 63912-64913 (Clockwise)

Preceding gene: 197116452

Following gene: 197116455

Centisome position: 1.38

GC content: 64.67

Gene sequence:

>1002_bases
ATGAAACGGGAGAAGCTAGTTGGGAAAGCTTTACTGCTGTTAGCGCTGGGCTCGGTGGGGACCTTGTCCGGATGTGCACA
CTACGAAGTAAACAACGGCCGCGGAAACATCCCGGGCTACTGGATCCGCTACGAGATGCAGGAGGCCGACCGCGCCATCG
AGGCTGCGCGCGCCGACGGGAAAGCCGCCCTCTGTCCTGATCAATTCAGGGAGGCCGAGGCGGCGAAGAACAACGCCTAC
GACGTCTTCCGGGCCTGCCACACCGAGGAGGGGGCCGCCCTGGCGAAAGAGGCGACGGCGAAGGCCAAAGCGCTCTGCCC
GCCGAGACCGGCCGAGCCCGCTCCCAAGCCGGTGGTCGCTCCGCCGCCACCGCCACCGCCTGCGCCCAAGGCTCCTACCG
ACACGCTGCTGGTCGAACCGGCTTCCGTGACCAAGGGTGAGACCGCCACGCTCAGCTGGACTTCCGCCAACGCCAACACC
TGCGCCATCGAGCCGGGTATCGGGCCGGTCCAGACCGCCGGTACTCTCACCATCACGCCGAAGGACGATATCCTCTACAC
CCTCGTCTGCACAGGCCCCGGCGGCGCGGCAAGAAGCGATGCCAAGGTGGCGGTGACAGCTCCCCCGGTCCCTGTCCCGG
TGCCGCCGGTCAAACTGTGCAAGCCGGCGGTGCTCAACGTCCATTTCGACACCAACAAGTCCGACATCAAGCCTCAGTAC
CATGATGAGCTTAAGGCCCTGGCGGACTTCCTGAAGGAGTTCCCGAACGCCACCGGGACCATCGAGGGTCACACCGACAG
CGTCGGCGACAAGGCCGCCAACATGAAGCTTTCTCAGCGTCGTGCCGACAGTGTCAGAAAATACCTGGTCGACAAGTTCG
GCATCGCACCCGAGCGCGTGAAGGCGGTGGGCTACGGCCCGACCAAGCCGGCGGCCGACAACAAGACCGCTGCAGGAAAG
CAGAAGAACCGCCGCATCGAGTCGAACTTCCATTGCGACTAA

Upstream 100 bases:

>100_bases
TCTGAACTTCGCAAGGTTACGCGGGACAAGGCGATTATCGTCCAGGCTCTGTCGGCTCAATGCAATGCCGGCACTGAATT
TAATGCACAAGGAGGCGAAG

Downstream 100 bases:

>100_bases
ATAGTCGGATTCTAGTCTGGTACCAGCATCTTCCAGCTCTCCCCCTCCCTTGACGGGCCTGCGCGCCGTAGCGCTTCGGT
CCGCGAAGGCGGGAGGGGGG

Product: OmpA family outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKREKLVGKALLLLALGSVGTLSGCAHYEVNNGRGNIPGYWIRYEMQEADRAIEAARADGKAALCPDQFREAEAAKNNAY
DVFRACHTEEGAALAKEATAKAKALCPPRPAEPAPKPVVAPPPPPPPAPKAPTDTLLVEPASVTKGETATLSWTSANANT
CAIEPGIGPVQTAGTLTITPKDDILYTLVCTGPGGAARSDAKVAVTAPPVPVPVPPVKLCKPAVLNVHFDTNKSDIKPQY
HDELKALADFLKEFPNATGTIEGHTDSVGDKAANMKLSQRRADSVRKYLVDKFGIAPERVKAVGYGPTKPAADNKTAAGK
QKNRRIESNFHCD

Sequences:

>Translated_333_residues
MKREKLVGKALLLLALGSVGTLSGCAHYEVNNGRGNIPGYWIRYEMQEADRAIEAARADGKAALCPDQFREAEAAKNNAY
DVFRACHTEEGAALAKEATAKAKALCPPRPAEPAPKPVVAPPPPPPPAPKAPTDTLLVEPASVTKGETATLSWTSANANT
CAIEPGIGPVQTAGTLTITPKDDILYTLVCTGPGGAARSDAKVAVTAPPVPVPVPPVKLCKPAVLNVHFDTNKSDIKPQY
HDELKALADFLKEFPNATGTIEGHTDSVGDKAANMKLSQRRADSVRKYLVDKFGIAPERVKAVGYGPTKPAADNKTAAGK
QKNRRIESNFHCD
>Mature_333_residues
MKREKLVGKALLLLALGSVGTLSGCAHYEVNNGRGNIPGYWIRYEMQEADRAIEAARADGKAALCPDQFREAEAAKNNAY
DVFRACHTEEGAALAKEATAKAKALCPPRPAEPAPKPVVAPPPPPPPAPKAPTDTLLVEPASVTKGETATLSWTSANANT
CAIEPGIGPVQTAGTLTITPKDDILYTLVCTGPGGAARSDAKVAVTAPPVPVPVPPVKLCKPAVLNVHFDTNKSDIKPQY
HDELKALADFLKEFPNATGTIEGHTDSVGDKAANMKLSQRRADSVRKYLVDKFGIAPERVKAVGYGPTKPAADNKTAAGK
QKNRRIESNFHCD

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=102, Percent_Identity=43.1372549019608, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=39.3939393939394, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 35240; Mature: 35240

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREKLVGKALLLLALGSVGTLSGCAHYEVNNGRGNIPGYWIRYEMQEADRAIEAARADG
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCC
KAALCPDQFREAEAAKNNAYDVFRACHTEEGAALAKEATAKAKALCPPRPAEPAPKPVVA
CCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PPPPPPPAPKAPTDTLLVEPASVTKGETATLSWTSANANTCAIEPGIGPVQTAGTLTITP
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCEEEECC
KDDILYTLVCTGPGGAARSDAKVAVTAPPVPVPVPPVKLCKPAVLNVHFDTNKSDIKPQY
CCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCHH
HDELKALADFLKEFPNATGTIEGHTDSVGDKAANMKLSQRRADSVRKYLVDKFGIAPERV
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHH
KAVGYGPTKPAADNKTAAGKQKNRRIESNFHCD
EEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
>Mature Secondary Structure
MKREKLVGKALLLLALGSVGTLSGCAHYEVNNGRGNIPGYWIRYEMQEADRAIEAARADG
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCC
KAALCPDQFREAEAAKNNAYDVFRACHTEEGAALAKEATAKAKALCPPRPAEPAPKPVVA
CCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PPPPPPPAPKAPTDTLLVEPASVTKGETATLSWTSANANTCAIEPGIGPVQTAGTLTITP
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCEEEECC
KDDILYTLVCTGPGGAARSDAKVAVTAPPVPVPVPPVKLCKPAVLNVHFDTNKSDIKPQY
CCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCHH
HDELKALADFLKEFPNATGTIEGHTDSVGDKAANMKLSQRRADSVRKYLVDKFGIAPERV
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHH
KAVGYGPTKPAADNKTAAGKQKNRRIESNFHCD
EEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]