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The map label for this gene is glu [H]

Identifier: 197106301

GI number: 197106301

Start: 3254991

End: 3255857

Strand: Direct

Name: glu [H]

Synonym: PHZ_c2840

Alternate gene names: 197106301

Gene position: 3254991-3255857 (Clockwise)

Preceding gene: 197106300

Following gene: 197106305

Centisome position: 81.45

GC content: 75.55

Gene sequence:

>867_bases
GTGTTCGTCACCCGCTTCGCCCCCTCGCCGACCGGCCTGCTGCACCGCGGCCACGCGTTCTCGGCGCTGACCGCCCACGA
GGCCGCGCGCGTGGTGGACGGCCGGTTCCTGCTGCGCATCGAGGACATCGACGCCACCCGCTGCCGGCCCGAGTACGAGG
CCGCCATCTACGAGGACCTGGCCTGGCTGGGGCTCGCGTGGGAGACGCCGGTGCGCCGCCAGTCCGAGCACCTGGACGAC
TACCAGGGCGCCCTGGAAAGGCTTGAGGCCGCCGGCCTGCTCTACCGCTGCTTCCGCACCCGCAAGGAGGTGGCGCAGGA
GATCGCCCGCGCTCCGCACGGGGCGATGGAGGTGTTCCGCGGCGCGCCCCTGCCGCGGGGCGAGGAGGCCCGGCGCCTGG
CGGCCGGCGAGCCCTTCGCCTGGCGCCTGTCGCTGGACGCCGCGGCCCGCGCGCTGGGCGGTTTCGGCGCCCTGACCTTC
GTCGAGGAGGGCTCGGGCGAGCCGCGCGTGGTGCAGGCCCGGCCCGAGCTGGGCGGCGACGTGGTGCTGGCCCGCAAGGA
CGTGGGCGTGGCCTACCACCTGGCCGTGGTGTTCGACGATGCGCTGCAGGGGATCACCCACGTGGTCCGCGGCGAGGACC
TGTTCGAGGCCACCCACGTCCAGCGCCTGCTGCAGGCCCTGCTCGGCCTGCCGACGCCGGTCTACCGGCACCACCGGCTG
CTGACCGGCCCGGACGGGCGCCGCTACGCCAAGCGGGACCGGTCCGAGACGCTGCGCGAACTCCGCGCCCGCGGTGTCAC
GCCGGAGGACCTGCGGCGGGAGATGGGCTTCGACGACCGACCGAAGCCCGCGAGCCGGCCTAGCTGA

Upstream 100 bases:

>100_bases
TTCAAGGCCCAGCTCGGCGGCGAGCGGGCGTTCTTCGAGGCGGTGCTGAACCACATGGGCCTGTTCCCCTCGGCGCACCG
CGACTGCTAGACGCCGGGCG

Downstream 100 bases:

>100_bases
GCCCCCACGCCTCGCGCGCCGTCTCGATCGCCGCCAGCTTGGCCTGGAACGGGGCCCAGTCGTCGGCTCCGGCGATGGGC
GCCCAGATCGCCTCGATCTC

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MFVTRFAPSPTGLLHRGHAFSALTAHEAARVVDGRFLLRIEDIDATRCRPEYEAAIYEDLAWLGLAWETPVRRQSEHLDD
YQGALERLEAAGLLYRCFRTRKEVAQEIARAPHGAMEVFRGAPLPRGEEARRLAAGEPFAWRLSLDAAARALGGFGALTF
VEEGSGEPRVVQARPELGGDVVLARKDVGVAYHLAVVFDDALQGITHVVRGEDLFEATHVQRLLQALLGLPTPVYRHHRL
LTGPDGRRYAKRDRSETLRELRARGVTPEDLRREMGFDDRPKPASRPS

Sequences:

>Translated_288_residues
MFVTRFAPSPTGLLHRGHAFSALTAHEAARVVDGRFLLRIEDIDATRCRPEYEAAIYEDLAWLGLAWETPVRRQSEHLDD
YQGALERLEAAGLLYRCFRTRKEVAQEIARAPHGAMEVFRGAPLPRGEEARRLAAGEPFAWRLSLDAAARALGGFGALTF
VEEGSGEPRVVQARPELGGDVVLARKDVGVAYHLAVVFDDALQGITHVVRGEDLFEATHVQRLLQALLGLPTPVYRHHRL
LTGPDGRRYAKRDRSETLRELRARGVTPEDLRREMGFDDRPKPASRPS
>Mature_288_residues
MFVTRFAPSPTGLLHRGHAFSALTAHEAARVVDGRFLLRIEDIDATRCRPEYEAAIYEDLAWLGLAWETPVRRQSEHLDD
YQGALERLEAAGLLYRCFRTRKEVAQEIARAPHGAMEVFRGAPLPRGEEARRLAAGEPFAWRLSLDAAARALGGFGALTF
VEEGSGEPRVVQARPELGGDVVLARKDVGVAYHLAVVFDDALQGITHVVRGEDLFEATHVQRLLQALLGLPTPVYRHHRL
LTGPDGRRYAKRDRSETLRELRARGVTPEDLRREMGFDDRPKPASRPS

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=283, Percent_Identity=32.5088339222615, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788743, Length=278, Percent_Identity=31.294964028777, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI226510925, Length=258, Percent_Identity=34.4961240310078, Blast_Score=118, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71993394, Length=266, Percent_Identity=29.6992481203008, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324540, Length=284, Percent_Identity=34.1549295774648, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24665321, Length=288, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 32171; Mature: 32171

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVTRFAPSPTGLLHRGHAFSALTAHEAARVVDGRFLLRIEDIDATRCRPEYEAAIYEDL
CEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCHHCCCHHHHHHHHHH
AWLGLAWETPVRRQSEHLDDYQGALERLEAAGLLYRCFRTRKEVAQEIARAPHGAMEVFR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GAPLPRGEEARRLAAGEPFAWRLSLDAAARALGGFGALTFVEEGSGEPRVVQARPELGGD
CCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHCCCHHEEEEECCCCCCEEEEECCCCCCC
VVLARKDVGVAYHLAVVFDDALQGITHVVRGEDLFEATHVQRLLQALLGLPTPVYRHHRL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCEE
LTGPDGRRYAKRDRSETLRELRARGVTPEDLRREMGFDDRPKPASRPS
EECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFVTRFAPSPTGLLHRGHAFSALTAHEAARVVDGRFLLRIEDIDATRCRPEYEAAIYEDL
CEEECCCCCCCCHHCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCHHCCCHHHHHHHHHH
AWLGLAWETPVRRQSEHLDDYQGALERLEAAGLLYRCFRTRKEVAQEIARAPHGAMEVFR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GAPLPRGEEARRLAAGEPFAWRLSLDAAARALGGFGALTFVEEGSGEPRVVQARPELGGD
CCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHCCCHHEEEEECCCCCCEEEEECCCCCCC
VVLARKDVGVAYHLAVVFDDALQGITHVVRGEDLFEATHVQRLLQALLGLPTPVYRHHRL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCEE
LTGPDGRRYAKRDRSETLRELRARGVTPEDLRREMGFDDRPKPASRPS
EECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA