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The map label for this gene is proP [H]

Identifier: 197106281

GI number: 197106281

Start: 3231994

End: 3233667

Strand: Direct

Name: proP [H]

Synonym: PHZ_c2820

Alternate gene names: 197106281

Gene position: 3231994-3233667 (Clockwise)

Preceding gene: 197106280

Following gene: 197106282

Centisome position: 80.88

GC content: 67.62

Gene sequence:

>1674_bases
ATGTCCAACGCGACCTATCCCGCGTCGGCGGCCGGCGACGAACACACGGTCGATCGGGACGATCGGCTGGTGATCGGCGC
CTCGTCCGTCGGCACGGTCTTCGAGTGGTACGACTTCTATCTGTACGGATCGCTCGCCAGCTACATCACCACCCACTTCT
TCTCCGGCGTGAACGAGACCACGGGCTACATCTTCGCCCTGATGGCCTTCGCCGCCGGCTTCGCCGTCCGGCCCTTCGGG
GCGCTGGTGTTCGGCCGTCTGGGCGACCTGTGGGGCCGCAAGAACACCTTCCTCGTGACCATGCTGCTGATGGGCCTGTC
GACCTTCGCGGTGGGCCTCTTGCCCTCGTACGCCCAGATCGGCGTCGCAGCGCCCGCCATCCTGATCGGCCTGCGGCTGC
TGCAGGGCCTGGCGCTCGGCGGCGAGTACGGCGGGGCCGCGACCTACGTCGCCGAGCACGCGCCGCACGGCAAGCGCGGC
CTCTACACCAGCTGGATCCAGACGACCGCCACCATGGGCCTGTTCATGAGCCTGGCGATCATCCTGGCCACGCGCACCGG
CATGGGCGAGGAGGCGTTCGCCGACTGGGGCTGGCGCGTGCCGTTCCTGGTCTCGATCTTCCTGCTGGCCGTCTCGATGT
GGATCCGGCTGAAGCTGCAGGAGAGCCCCGCGTTCCAGCGCATGAAGGCCGAGGGCAAGGGCACCACCCAGCCCCTCAAG
GAGAGCTTCGCCCGCTGGGGGAACCTGAAGATCGTCGTCCTGGCGCTGGTGGGCCTGACCGCCGGCCAGGCCGTGGTCTG
GTACACCGGCCAGTTCTACGCCCTGTTCTTCCTCGAGAAGATGCTGAAGGTCGACGGGGCGCTGACCAACACCCTAGTGG
CCCTGGCGCTGCTGATCGGCACGCCGTTCTTCATCTTCTTCGGCTGGCTGTCCGACAAGATCGGGCGGAAGAAGATCATC
ATGGCCGGCTGCCTGATCGCGGCCCTGACCTACTTCCCGCTGTTCAAGGCGCTGACCTGGGCCGCGAACCCGGACCTCGC
CCGCGCCGCGGCCACCAGCCCGGTGACGGTCGTCGCCGACCCGGCCGACTGCTCGGTCCAGTTCGACCCCGTCGGCAAGA
CGGTGTTCAACAAGAGCTGCGACATCGCCAAGTCGTACTTGGCCAAGGCCGGCGTCACCTACCAGAACGAGGCGGCCCCG
GCCGGCTCGATCGCCCAGGTGCGGGTCGGCGACGAAGTCATCGCCTCGTTCCGGGGCGAGGCCCTGCCCAAGGCCGATTT
CGCGGCCCAGAAAGGCGCCTGGGAAAAGAACATGGGCGCGGTGCTGAGCGCCGCGGGCTACCCGGCCAAGGCGGACTCCG
CCAAGGTCAACAAGGTGGCCGTGGTCGGCATCCTGACCCTGCTCGTGATCTACGTGACCATGGTCTACGGCCCGATCGCC
GCCATGCTGGTGGAGCTGTTCCCCACCCGGATCCGCTACACCTCGATGAGCCTGCCCTACCACATCGGCAACGGCTGGTT
CGGCGGCTTCCTGCCCACCACGGCGTTCGCCATGGTCGCGGCCACGGGCAACATCTACTACGGCCTCTGGTACCCGGTGG
TGGTGGCCGCGGTCACCTTCGTGATCGGCGTGCTGTTCATCCGGGAGACCAAAGACGTCGACATCCACGCCTGA

Upstream 100 bases:

>100_bases
CGGGCAAGGCCAAGCGTGCAGGGTTCGGCTTTGAGGGCGGAGGGGGGACCTCCGTCGGCATGGCGGCCGTCCGCAAGCGA
CGGCGCCCTGGGAGGAGCCT

Downstream 100 bases:

>100_bases
GTAAGCCCGTCTTACCTCCCCTGAGGCGTGCGCGGGCGGCCGAAAGGCCGCCCGCAAGCTTTTCTGCGACCTTCGTCGGC
TTGCGGAACGGCCGCGGCGG

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSNATYPASAAGDEHTVDRDDRLVIGASSVGTVFEWYDFYLYGSLASYITTHFFSGVNETTGYIFALMAFAAGFAVRPFG
ALVFGRLGDLWGRKNTFLVTMLLMGLSTFAVGLLPSYAQIGVAAPAILIGLRLLQGLALGGEYGGAATYVAEHAPHGKRG
LYTSWIQTTATMGLFMSLAIILATRTGMGEEAFADWGWRVPFLVSIFLLAVSMWIRLKLQESPAFQRMKAEGKGTTQPLK
ESFARWGNLKIVVLALVGLTAGQAVVWYTGQFYALFFLEKMLKVDGALTNTLVALALLIGTPFFIFFGWLSDKIGRKKII
MAGCLIAALTYFPLFKALTWAANPDLARAAATSPVTVVADPADCSVQFDPVGKTVFNKSCDIAKSYLAKAGVTYQNEAAP
AGSIAQVRVGDEVIASFRGEALPKADFAAQKGAWEKNMGAVLSAAGYPAKADSAKVNKVAVVGILTLLVIYVTMVYGPIA
AMLVELFPTRIRYTSMSLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVVVAAVTFVIGVLFIRETKDVDIHA

Sequences:

>Translated_557_residues
MSNATYPASAAGDEHTVDRDDRLVIGASSVGTVFEWYDFYLYGSLASYITTHFFSGVNETTGYIFALMAFAAGFAVRPFG
ALVFGRLGDLWGRKNTFLVTMLLMGLSTFAVGLLPSYAQIGVAAPAILIGLRLLQGLALGGEYGGAATYVAEHAPHGKRG
LYTSWIQTTATMGLFMSLAIILATRTGMGEEAFADWGWRVPFLVSIFLLAVSMWIRLKLQESPAFQRMKAEGKGTTQPLK
ESFARWGNLKIVVLALVGLTAGQAVVWYTGQFYALFFLEKMLKVDGALTNTLVALALLIGTPFFIFFGWLSDKIGRKKII
MAGCLIAALTYFPLFKALTWAANPDLARAAATSPVTVVADPADCSVQFDPVGKTVFNKSCDIAKSYLAKAGVTYQNEAAP
AGSIAQVRVGDEVIASFRGEALPKADFAAQKGAWEKNMGAVLSAAGYPAKADSAKVNKVAVVGILTLLVIYVTMVYGPIA
AMLVELFPTRIRYTSMSLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVVVAAVTFVIGVLFIRETKDVDIHA
>Mature_556_residues
SNATYPASAAGDEHTVDRDDRLVIGASSVGTVFEWYDFYLYGSLASYITTHFFSGVNETTGYIFALMAFAAGFAVRPFGA
LVFGRLGDLWGRKNTFLVTMLLMGLSTFAVGLLPSYAQIGVAAPAILIGLRLLQGLALGGEYGGAATYVAEHAPHGKRGL
YTSWIQTTATMGLFMSLAIILATRTGMGEEAFADWGWRVPFLVSIFLLAVSMWIRLKLQESPAFQRMKAEGKGTTQPLKE
SFARWGNLKIVVLALVGLTAGQAVVWYTGQFYALFFLEKMLKVDGALTNTLVALALLIGTPFFIFFGWLSDKIGRKKIIM
AGCLIAALTYFPLFKALTWAANPDLARAAATSPVTVVADPADCSVQFDPVGKTVFNKSCDIAKSYLAKAGVTYQNEAAPA
GSIAQVRVGDEVIASFRGEALPKADFAAQKGAWEKNMGAVLSAAGYPAKADSAKVNKVAVVGILTLLVIYVTMVYGPIAA
MLVELFPTRIRYTSMSLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVVVAAVTFVIGVLFIRETKDVDIHA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=329, Percent_Identity=33.7386018237082, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788292, Length=336, Percent_Identity=35.1190476190476, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=344, Percent_Identity=32.2674418604651, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789941, Length=343, Percent_Identity=34.9854227405248, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI87082231, Length=247, Percent_Identity=25.1012145748988, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=24, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59924; Mature: 59792

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNATYPASAAGDEHTVDRDDRLVIGASSVGTVFEWYDFYLYGSLASYITTHFFSGVNET
CCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TGYIFALMAFAAGFAVRPFGALVFGRLGDLWGRKNTFLVTMLLMGLSTFAVGLLPSYAQI
HHHHHHHHHHHCCCHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
GVAAPAILIGLRLLQGLALGGEYGGAATYVAEHAPHGKRGLYTSWIQTTATMGLFMSLAI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILATRTGMGEEAFADWGWRVPFLVSIFLLAVSMWIRLKLQESPAFQRMKAEGKGTTQPLK
HHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCHHHH
ESFARWGNLKIVVLALVGLTAGQAVVWYTGQFYALFFLEKMLKVDGALTNTLVALALLIG
HHHHHHCCCCEEEHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TPFFIFFGWLSDKIGRKKIIMAGCLIAALTYFPLFKALTWAANPDLARAAATSPVTVVAD
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEC
PADCSVQFDPVGKTVFNKSCDIAKSYLAKAGVTYQNEAAPAGSIAQVRVGDEVIASFRGE
CCCCEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHCCC
ALPKADFAAQKGAWEKNMGAVLSAAGYPAKADSAKVNKVAVVGILTLLVIYVTMVYGPIA
CCCCHHHHHHCCCCHHCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AMLVELFPTRIRYTSMSLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVVVAAVTF
HHHHHHHHHHHEEEEECCCEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
VIGVLFIRETKDVDIHA
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNATYPASAAGDEHTVDRDDRLVIGASSVGTVFEWYDFYLYGSLASYITTHFFSGVNET
CCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
TGYIFALMAFAAGFAVRPFGALVFGRLGDLWGRKNTFLVTMLLMGLSTFAVGLLPSYAQI
HHHHHHHHHHHCCCHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
GVAAPAILIGLRLLQGLALGGEYGGAATYVAEHAPHGKRGLYTSWIQTTATMGLFMSLAI
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILATRTGMGEEAFADWGWRVPFLVSIFLLAVSMWIRLKLQESPAFQRMKAEGKGTTQPLK
HHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCHHHH
ESFARWGNLKIVVLALVGLTAGQAVVWYTGQFYALFFLEKMLKVDGALTNTLVALALLIG
HHHHHHCCCCEEEHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TPFFIFFGWLSDKIGRKKIIMAGCLIAALTYFPLFKALTWAANPDLARAAATSPVTVVAD
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEC
PADCSVQFDPVGKTVFNKSCDIAKSYLAKAGVTYQNEAAPAGSIAQVRVGDEVIASFRGE
CCCCEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHCCC
ALPKADFAAQKGAWEKNMGAVLSAAGYPAKADSAKVNKVAVVGILTLLVIYVTMVYGPIA
CCCCHHHHHHCCCCHHCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AMLVELFPTRIRYTSMSLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVVVAAVTF
HHHHHHHHHHHEEEEECCCEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
VIGVLFIRETKDVDIHA
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]