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The map label for this gene is greA

Identifier: 197106054

GI number: 197106054

Start: 2981040

End: 2981513

Strand: Direct

Name: greA

Synonym: PHZ_c2593

Alternate gene names: 197106054

Gene position: 2981040-2981513 (Clockwise)

Preceding gene: 197106052

Following gene: 197106055

Centisome position: 74.6

GC content: 66.03

Gene sequence:

>474_bases
ATGGAAAAAGTCCCGATGACCGCCGAGGGCTACAAGGCCCTCGACGAAGAGCTGAAGCGTTTGAAGACGGTGGAGCGGCC
GGCTGTGATCGCCGCTATCGCCGAGGCGCGCTCGCACGGCGATCTCTCGGAGAACGCGGAGTATCACGCCGCCAAGGAGC
GCCAGGGCTGGATCGAAGGCCAGATCGCCGAGATCGAGGACAAGATGGCCCGCGCCCAGGTGATCGACGTGTCCAAGCTG
TCGGGCGAACAGGTCAAGTTCGGCGCCACGGTCTCGCTCATCGACGAGGACACCGAGGAAGAGGCCCGCTACCAGATCGT
CGGCGAGCACGAGGCCGACGTGAAGGCCGGCCGCGTCTCGGTCACCTCGCCCATCGCCCGCGCCATCATCGGCAAGGAGA
CCGGCGACGTCGTGGAGGTGAACACCCCCGGCGGCGTGAAGGCCTACGAGATCACCAAGGTGGAGTGGGTCTGA

Upstream 100 bases:

>100_bases
ATTTCGCAGCGCACAATCCTAACCTTTCCGCCCCCGGACGCCTGCGCCCGCGGCCGGCCTACCGACAACTCACCTAGGGC
GAACTCGAGTCATCACCTCT

Downstream 100 bases:

>100_bases
CCCACGCCTGAATGGCGACGGCGAGCCCGCCTCCCCTGACCATCTGGGCCGTCTCGGACGGCCGGGCGGGCATCGAGGCC
CAGGCCCTGGGCCTCGCCGA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MEKVPMTAEGYKALDEELKRLKTVERPAVIAAIAEARSHGDLSENAEYHAAKERQGWIEGQIAEIEDKMARAQVIDVSKL
SGEQVKFGATVSLIDEDTEEEARYQIVGEHEADVKAGRVSVTSPIARAIIGKETGDVVEVNTPGGVKAYEITKVEWV

Sequences:

>Translated_157_residues
MEKVPMTAEGYKALDEELKRLKTVERPAVIAAIAEARSHGDLSENAEYHAAKERQGWIEGQIAEIEDKMARAQVIDVSKL
SGEQVKFGATVSLIDEDTEEEARYQIVGEHEADVKAGRVSVTSPIARAIIGKETGDVVEVNTPGGVKAYEITKVEWV
>Mature_157_residues
MEKVPMTAEGYKALDEELKRLKTVERPAVIAAIAEARSHGDLSENAEYHAAKERQGWIEGQIAEIEDKMARAQVIDVSKL
SGEQVKFGATVSLIDEDTEEEARYQIVGEHEADVKAGRVSVTSPIARAIIGKETGDVVEVNTPGGVKAYEITKVEWV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=56.3291139240506, Blast_Score=180, Evalue=5e-47,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=36.5384615384615, Blast_Score=93, Evalue=9e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_PHEZH (B4RH54)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002131431.1
- GeneID:   6792115
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c2593
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17177; Mature: 17177

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVPMTAEGYKALDEELKRLKTVERPAVIAAIAEARSHGDLSENAEYHAAKERQGWIEG
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
QIAEIEDKMARAQVIDVSKLSGEQVKFGATVSLIDEDTEEEARYQIVGEHEADVKAGRVS
HHHHHHHHHHHHHHEEHHHCCCCEEEECCEEEEECCCCCCCCCEEEECCCCCCCCCCEEE
VTSPIARAIIGKETGDVVEVNTPGGVKAYEITKVEWV
EHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEC
>Mature Secondary Structure
MEKVPMTAEGYKALDEELKRLKTVERPAVIAAIAEARSHGDLSENAEYHAAKERQGWIEG
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
QIAEIEDKMARAQVIDVSKLSGEQVKFGATVSLIDEDTEEEARYQIVGEHEADVKAGRVS
HHHHHHHHHHHHHHEEHHHCCCCEEEECCEEEEECCCCCCCCCEEEECCCCCCCCCCEEE
VTSPIARAIIGKETGDVVEVNTPGGVKAYEITKVEWV
EHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA