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The map label for this gene is emrB [H]

Identifier: 197106026

GI number: 197106026

Start: 2946458

End: 2948077

Strand: Direct

Name: emrB [H]

Synonym: PHZ_c2565

Alternate gene names: 197106026

Gene position: 2946458-2948077 (Clockwise)

Preceding gene: 197106023

Following gene: 197106029

Centisome position: 73.73

GC content: 67.78

Gene sequence:

>1620_bases
GTGAACCGCATGAACGCCACCACCCCGACCCCGCCCGACGTTCCGCATGGGGACGTGACGGTCGCCCGGCCGCCGCGAAA
GCCAGACGGCGCGGTCGACTACCTTCGGCTCTACATCGGCTTCGGGGCCATGGTTCTTGGCCAGTTCATGGCGCTGCTGG
ACGTGCAGATCGTCGCCGCGTCGCTGCCCCACATCCAGACTGGCATTGACGCCTCCGCCGACCAGATCAGCTGGATTCAG
ACGACCTACCTCGTGGCCGAGGTGATCGGCATTCCGCTGTCGGGTTACCTTTCCCGACTGTACGGCACCCAGAAGCTGTA
TCTTCTCGCCTGCGCCGCCTTCATCGCCATGAGCGTCCTCGCCGGCTTGTCCACCAGCCTCGAGGTGATGCTCTTCGCAC
GCGCGCTTCAGGGTTTCGCTGGCGGCCTCCTGATCCCGACCGTCTATGCCATCGCCTTCACGGCGTTCCCGGAGAAGGAT
CGAGGAACGGCCAACATCCTGATGGGCATGATGCTCAGCCTGTCGCCGACGCTGGGTCCGACGCTGGGCGGCTACGTGAC
CGAGTGGCTGTCCTGGCGGTGGGTCTTCCTGATCAACGTCTTCCCCGGGGCGCTCGTGCTGGTCTGCGTGGCCCGATACG
GTTCGTTCGACAGCGGCGACAGCCGCCTCGCGAAGGGATTCGACTGGTCCGGCCTCGTGCTGATGGCCGCCTTCCTCGTG
AGCATCCAGTACGTGGTGGAGGAAGGCCCCAAGGACGGCTGGTTCGAGGACGACCTGATCCTGTGGCTCACGGTCCTCGC
CGGCCTCACGGGGGCGGCCTTCCTCTGGCGGCAGCTCAGTTGCGTCCAGCCGATCCTGGAACTGAGGGCCTTCCGCGATC
GCAACTTCGCCATCGGCGTCGTCCTCACGGCCGTCTCGGGCGCGGGATTGTTCGGCTCGACGTTCCTGCTGCCGCTCTTC
CTGGCGCGCGTTCAGGACTTCTCCCCCGTCCAGATCGGGCTGACGATGGTCGTGTCGGGCGCCACCATGTTCCTGGCGGG
TCCCTTGCTGCGCGCGTTCGTCCTCACGCGAGTGGATCCCCGTTTCGCGATCGTCGCCGGCTTCGGCCTGTCCGCCATGG
GCCTCTGGCTCGGACGGAATGTGACGGAGGATTGGGGATTCAACGAATTCGCGCTGCTGCAGTTCTGGCGCAGCCTGGGC
GTGATCATGGCCATCGTGGGCGTCCAGGGCGTGACGCTCGCCACCCTTTCCCCACGGCTCGTGAAGAGCGGGTCCAGCCT
GGTCAACCTCTCCCGGATGATCGGCGGCTCCATCGGCCTGGCGATGCTTTCCTCGACCCTGGCGTACCAGACGGCGCTGC
ACGCGAGCGACCTCGCGGCGGCCATTCCGGTAGGGTCCGCTCGCGCGGAAACGGCCCTCGCCGTCCTGACCGGGCGCCTT
GAAGCGGCCGGCAGCCACGCGCCGGCCGAGGGAGCGCTTGCGCTCCTCGGCGCCGCGCTCGGCCGCGAAGCGACGATCCT
GGCCTTCGGTGACGCCTTCGCTCTGCTCGCGCTGGTGACCGCCGCGGCGGCCTGCCTGGGCCTGCTGGTCACCGCCAAGT
CGAGGAACACCGGCCTGTAG

Upstream 100 bases:

>100_bases
ACTCCGGCATGGGTCGCTCCTCCAGTCGACGGCCAATGGCGCCGTCACCAAGAAATAGTTCGATCAGTTAGATAATGGAA
GGCATGATCGCAGCCTGACT

Downstream 100 bases:

>100_bases
CCGACGGTGGGGAGCCCATCCCACCCTTCATTCAGGGCCGTCTCAAAGCACTGTGTCGGCCGCGGCGCACATCACCGCGG
AGCCGTCGGCGGCGCAGGCC

Product: drug resistance transporter, EmrB/QacA subfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MNRMNATTPTPPDVPHGDVTVARPPRKPDGAVDYLRLYIGFGAMVLGQFMALLDVQIVAASLPHIQTGIDASADQISWIQ
TTYLVAEVIGIPLSGYLSRLYGTQKLYLLACAAFIAMSVLAGLSTSLEVMLFARALQGFAGGLLIPTVYAIAFTAFPEKD
RGTANILMGMMLSLSPTLGPTLGGYVTEWLSWRWVFLINVFPGALVLVCVARYGSFDSGDSRLAKGFDWSGLVLMAAFLV
SIQYVVEEGPKDGWFEDDLILWLTVLAGLTGAAFLWRQLSCVQPILELRAFRDRNFAIGVVLTAVSGAGLFGSTFLLPLF
LARVQDFSPVQIGLTMVVSGATMFLAGPLLRAFVLTRVDPRFAIVAGFGLSAMGLWLGRNVTEDWGFNEFALLQFWRSLG
VIMAIVGVQGVTLATLSPRLVKSGSSLVNLSRMIGGSIGLAMLSSTLAYQTALHASDLAAAIPVGSARAETALAVLTGRL
EAAGSHAPAEGALALLGAALGREATILAFGDAFALLALVTAAAACLGLLVTAKSRNTGL

Sequences:

>Translated_539_residues
MNRMNATTPTPPDVPHGDVTVARPPRKPDGAVDYLRLYIGFGAMVLGQFMALLDVQIVAASLPHIQTGIDASADQISWIQ
TTYLVAEVIGIPLSGYLSRLYGTQKLYLLACAAFIAMSVLAGLSTSLEVMLFARALQGFAGGLLIPTVYAIAFTAFPEKD
RGTANILMGMMLSLSPTLGPTLGGYVTEWLSWRWVFLINVFPGALVLVCVARYGSFDSGDSRLAKGFDWSGLVLMAAFLV
SIQYVVEEGPKDGWFEDDLILWLTVLAGLTGAAFLWRQLSCVQPILELRAFRDRNFAIGVVLTAVSGAGLFGSTFLLPLF
LARVQDFSPVQIGLTMVVSGATMFLAGPLLRAFVLTRVDPRFAIVAGFGLSAMGLWLGRNVTEDWGFNEFALLQFWRSLG
VIMAIVGVQGVTLATLSPRLVKSGSSLVNLSRMIGGSIGLAMLSSTLAYQTALHASDLAAAIPVGSARAETALAVLTGRL
EAAGSHAPAEGALALLGAALGREATILAFGDAFALLALVTAAAACLGLLVTAKSRNTGL
>Mature_539_residues
MNRMNATTPTPPDVPHGDVTVARPPRKPDGAVDYLRLYIGFGAMVLGQFMALLDVQIVAASLPHIQTGIDASADQISWIQ
TTYLVAEVIGIPLSGYLSRLYGTQKLYLLACAAFIAMSVLAGLSTSLEVMLFARALQGFAGGLLIPTVYAIAFTAFPEKD
RGTANILMGMMLSLSPTLGPTLGGYVTEWLSWRWVFLINVFPGALVLVCVARYGSFDSGDSRLAKGFDWSGLVLMAAFLV
SIQYVVEEGPKDGWFEDDLILWLTVLAGLTGAAFLWRQLSCVQPILELRAFRDRNFAIGVVLTAVSGAGLFGSTFLLPLF
LARVQDFSPVQIGLTMVVSGATMFLAGPLLRAFVLTRVDPRFAIVAGFGLSAMGLWLGRNVTEDWGFNEFALLQFWRSLG
VIMAIVGVQGVTLATLSPRLVKSGSSLVNLSRMIGGSIGLAMLSSTLAYQTALHASDLAAAIPVGSARAETALAVLTGRL
EAAGSHAPAEGALALLGAALGREATILAFGDAFALLALVTAAAACLGLLVTAKSRNTGL

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=455, Percent_Identity=28.5714285714286, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788710, Length=424, Percent_Identity=29.2452830188679, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081983, Length=314, Percent_Identity=27.7070063694268, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790195, Length=408, Percent_Identity=25.9803921568627, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1788392, Length=407, Percent_Identity=26.044226044226, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=180, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=27.007299270073, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56947; Mature: 56947

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRMNATTPTPPDVPHGDVTVARPPRKPDGAVDYLRLYIGFGAMVLGQFMALLDVQIVAA
CCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLPHIQTGIDASADQISWIQTTYLVAEVIGIPLSGYLSRLYGTQKLYLLACAAFIAMSVL
HCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AGLSTSLEVMLFARALQGFAGGLLIPTVYAIAFTAFPEKDRGTANILMGMMLSLSPTLGP
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
TLGGYVTEWLSWRWVFLINVFPGALVLVCVARYGSFDSGDSRLAKGFDWSGLVLMAAFLV
HHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHH
SIQYVVEEGPKDGWFEDDLILWLTVLAGLTGAAFLWRQLSCVQPILELRAFRDRNFAIGV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
VLTAVSGAGLFGSTFLLPLFLARVQDFSPVQIGLTMVVSGATMFLAGPLLRAFVLTRVDP
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
RFAIVAGFGLSAMGLWLGRNVTEDWGFNEFALLQFWRSLGVIMAIVGVQGVTLATLSPRL
CEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
VKSGSSLVNLSRMIGGSIGLAMLSSTLAYQTALHASDLAAAIPVGSARAETALAVLTGRL
HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EAAGSHAPAEGALALLGAALGREATILAFGDAFALLALVTAAAACLGLLVTAKSRNTGL
HHCCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MNRMNATTPTPPDVPHGDVTVARPPRKPDGAVDYLRLYIGFGAMVLGQFMALLDVQIVAA
CCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLPHIQTGIDASADQISWIQTTYLVAEVIGIPLSGYLSRLYGTQKLYLLACAAFIAMSVL
HCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AGLSTSLEVMLFARALQGFAGGLLIPTVYAIAFTAFPEKDRGTANILMGMMLSLSPTLGP
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
TLGGYVTEWLSWRWVFLINVFPGALVLVCVARYGSFDSGDSRLAKGFDWSGLVLMAAFLV
HHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHH
SIQYVVEEGPKDGWFEDDLILWLTVLAGLTGAAFLWRQLSCVQPILELRAFRDRNFAIGV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
VLTAVSGAGLFGSTFLLPLFLARVQDFSPVQIGLTMVVSGATMFLAGPLLRAFVLTRVDP
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
RFAIVAGFGLSAMGLWLGRNVTEDWGFNEFALLQFWRSLGVIMAIVGVQGVTLATLSPRL
CEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
VKSGSSLVNLSRMIGGSIGLAMLSSTLAYQTALHASDLAAAIPVGSARAETALAVLTGRL
HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EAAGSHAPAEGALALLGAALGREATILAFGDAFALLALVTAAAACLGLLVTAKSRNTGL
HHCCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]