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The map label for this gene is yngF [H]

Identifier: 197106015

GI number: 197106015

Start: 2933985

End: 2934818

Strand: Reverse

Name: yngF [H]

Synonym: PHZ_c2554

Alternate gene names: 197106015

Gene position: 2934818-2933985 (Counterclockwise)

Preceding gene: 197106016

Following gene: 197106014

Centisome position: 73.44

GC content: 70.62

Gene sequence:

>834_bases
GTGAACGACTTCGTTCAGTGCGAGATCTCGGAAGGCGTGGCCGTCGTCACGCTCAACCGGCCCGAGCGCCTCAATGCGAT
CGGCCCGGCCATGGGCGACCTGTTCGATCGGACCATGGTGCAGGTCGGGCTGGACGACGCCGTCAAGGTCGTCGTGCTCA
CCGGCAGCGGCCGGGGCTTCTGCGCCGGGGCGGACATGGAGCGCCTGGATGACCTCGCCGCCACGGCGGGCGGCTCGTTC
GAGCGGCTGCCCCCCGGATCGCCCCATCCCGTCTTCGACGCCCTCGCCGATTCGCCGCCGGAGCACCGCTCGCGGTACAT
CATTCCGAGCGCCTTGCCGAAGCCTGTGATCGCAGCGGTGAACGGCGCCTGCGCCGGCGTCGGCCTTTCGCTGGCGGCGT
CCTGCGACGTCAGGTTCGCGAGCACGGACGCGCTGTTCACTGCCGGCTTCCCGCGGCGAGGCCTGACGGCGGAAGCCGGG
CTCGCCTTCACGCTGCCGGCCATCGTCGGACAGGGGGCGGCGGCTGACATCCTGCTGTCCGGCCGACGCCTTTCCGCGCA
CGAGGCGCTGCGTATCGGACTGGTGAGCGTGGTCCTCGAGCCTGCGGACCTGCTGGCCCATGCGGTCGACTATGCGCGCG
ACATCGCCGAGAACGTCTCGCCGCGGTCCACGCGGGTCATCAAGCGCCAGCTGTGGAAGGCGCGCAGCGAGACGTTCGCC
GAGGCCCTGACGACGGCTTACGAGGAAGTCGTGGCGAGCCTCGAGTCCGAGGACTTCAAGGAGGGCGTCGCCCACTTCCG
CGAACGGCGCAAGCCGGTGTTCGTGGGGCGCTAG

Upstream 100 bases:

>100_bases
CTGTTCCTGGTCAGCGCAGCCGGACGGCATGTCACCGGCCAGACCCTGGTGGTCGACGGCGGCGCTACGCTGACCGCCGC
CAACTGAGAAGGGAGCCCCC

Downstream 100 bases:

>100_bases
GCATGACCGCCGACCCCCGCCGCGACCCGGTGGCCTATCCGATCGTCGGGGAGGTCCGCCCGCGCTACCGCGACGTCGAT
CCCCTCCGTCACCTGAACAA

Product: enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNDFVQCEISEGVAVVTLNRPERLNAIGPAMGDLFDRTMVQVGLDDAVKVVVLTGSGRGFCAGADMERLDDLAATAGGSF
ERLPPGSPHPVFDALADSPPEHRSRYIIPSALPKPVIAAVNGACAGVGLSLAASCDVRFASTDALFTAGFPRRGLTAEAG
LAFTLPAIVGQGAAADILLSGRRLSAHEALRIGLVSVVLEPADLLAHAVDYARDIAENVSPRSTRVIKRQLWKARSETFA
EALTTAYEEVVASLESEDFKEGVAHFRERRKPVFVGR

Sequences:

>Translated_277_residues
MNDFVQCEISEGVAVVTLNRPERLNAIGPAMGDLFDRTMVQVGLDDAVKVVVLTGSGRGFCAGADMERLDDLAATAGGSF
ERLPPGSPHPVFDALADSPPEHRSRYIIPSALPKPVIAAVNGACAGVGLSLAASCDVRFASTDALFTAGFPRRGLTAEAG
LAFTLPAIVGQGAAADILLSGRRLSAHEALRIGLVSVVLEPADLLAHAVDYARDIAENVSPRSTRVIKRQLWKARSETFA
EALTTAYEEVVASLESEDFKEGVAHFRERRKPVFVGR
>Mature_277_residues
MNDFVQCEISEGVAVVTLNRPERLNAIGPAMGDLFDRTMVQVGLDDAVKVVVLTGSGRGFCAGADMERLDDLAATAGGSF
ERLPPGSPHPVFDALADSPPEHRSRYIIPSALPKPVIAAVNGACAGVGLSLAASCDVRFASTDALFTAGFPRRGLTAEAG
LAFTLPAIVGQGAAADILLSGRRLSAHEALRIGLVSVVLEPADLLAHAVDYARDIAENVSPRSTRVIKRQLWKARSETFA
EALTTAYEEVVASLESEDFKEGVAHFRERRKPVFVGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=262, Percent_Identity=29.3893129770992, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI194097323, Length=262, Percent_Identity=30.9160305343511, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI45643119, Length=260, Percent_Identity=25.7692307692308, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI260275230, Length=260, Percent_Identity=25.7692307692308, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI260274832, Length=260, Percent_Identity=25.7692307692308, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI31542718, Length=267, Percent_Identity=27.7153558052434, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI20127408, Length=282, Percent_Identity=27.6595744680851, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221307494, Length=196, Percent_Identity=28.0612244897959, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI221136756, Length=196, Percent_Identity=28.0612244897959, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136753, Length=196, Percent_Identity=28.0612244897959, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4757968, Length=154, Percent_Identity=27.9220779220779, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI49355787, Length=154, Percent_Identity=27.9220779220779, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25453481, Length=154, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538573, Length=154, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757966, Length=154, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51538581, Length=154, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4502327, Length=273, Percent_Identity=25.2747252747253, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787660, Length=284, Percent_Identity=33.0985915492958, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787659, Length=270, Percent_Identity=32.962962962963, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=29.6992481203008, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI221142681, Length=270, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=28.5024154589372, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082183, Length=283, Percent_Identity=24.7349823321555, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=268, Percent_Identity=33.2089552238806, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=268, Percent_Identity=31.7164179104478, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=274, Percent_Identity=27.7372262773723, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=282, Percent_Identity=30.1418439716312, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=24.21875, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=27.8538812785388, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=219, Percent_Identity=28.7671232876712, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=230, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=240, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=267, Percent_Identity=27.3408239700375, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=290, Percent_Identity=30, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=290, Percent_Identity=30, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=29.7794117647059, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19920382, Length=271, Percent_Identity=30.9963099630996, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24654903, Length=263, Percent_Identity=27.3764258555133, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24650670, Length=267, Percent_Identity=29.9625468164794, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21357171, Length=274, Percent_Identity=25.9124087591241, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922422, Length=279, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921000, Length=204, Percent_Identity=28.921568627451, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24583077, Length=204, Percent_Identity=28.921568627451, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583079, Length=204, Percent_Identity=28.921568627451, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 29380; Mature: 29380

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFVQCEISEGVAVVTLNRPERLNAIGPAMGDLFDRTMVQVGLDDAVKVVVLTGSGRGF
CCCCEEEEECCCEEEEEECCCHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
CAGADMERLDDLAATAGGSFERLPPGSPHPVFDALADSPPEHRSRYIIPSALPKPVIAAV
ECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHCCEEECCCCCCCHHHHHH
NGACAGVGLSLAASCDVRFASTDALFTAGFPRRGLTAEAGLAFTLPAIVGQGAAADILLS
CCHHHHCCHHEEECCCEEEECCCCEEECCCCCCCCCCCCCHHEEHHHHHCCCCHHHHEEC
GRRLSAHEALRIGLVSVVLEPADLLAHAVDYARDIAENVSPRSTRVIKRQLWKARSETFA
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EALTTAYEEVVASLESEDFKEGVAHFRERRKPVFVGR
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNDFVQCEISEGVAVVTLNRPERLNAIGPAMGDLFDRTMVQVGLDDAVKVVVLTGSGRGF
CCCCEEEEECCCEEEEEECCCHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCE
CAGADMERLDDLAATAGGSFERLPPGSPHPVFDALADSPPEHRSRYIIPSALPKPVIAAV
ECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCHHHCCEEECCCCCCCHHHHHH
NGACAGVGLSLAASCDVRFASTDALFTAGFPRRGLTAEAGLAFTLPAIVGQGAAADILLS
CCHHHHCCHHEEECCCEEEECCCCEEECCCCCCCCCCCCCHHEEHHHHHCCCCHHHHEEC
GRRLSAHEALRIGLVSVVLEPADLLAHAVDYARDIAENVSPRSTRVIKRQLWKARSETFA
CCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
EALTTAYEEVVASLESEDFKEGVAHFRERRKPVFVGR
HHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]