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The map label for this gene is paaF [C]

Identifier: 197106010

GI number: 197106010

Start: 2926229

End: 2926999

Strand: Reverse

Name: paaF [C]

Synonym: PHZ_c2549

Alternate gene names: 197106010

Gene position: 2926999-2926229 (Counterclockwise)

Preceding gene: 197106011

Following gene: 197106009

Centisome position: 73.24

GC content: 71.85

Gene sequence:

>771_bases
ATGAGCGCGGACGTCCTCCTCGACCGCCCGGCGCCGCGCGTGGCGCGGATCACGCTCAACACGCCGGAAACCCGCAACGC
GGCCAGTCCAGGTCGCACCAAGCTCCTGCGGCAGCTCTTCGACCAGGCGCTGGACGACGAGACCGTCGGCGCCGTTGTGT
TCACCGGAGCCGGCGGCAACTTCGCCTCTGGCGGTAACGTGAAGGGCTTCGGGACGCGCGAGCCTATCGAGTTCCTCGGC
AGCGTGCGCCAGGCGCAGGCGCTGCTGCGGCGCGTGCTCCAGGGCGACAAGCCGGTGGTCGCGGCGGTCGAAGGTTTCGC
GGCGGGGGCCGGCGTCGGCATGGCGTGCATGTGCGACGTGATCGTCGCGGACGCGAATACGCGGTTCGTCTTCCCCTTCA
CCCGCATCGGCATGGTGCCGGACTTGGGGCTGCACTACACGCTCGGCCAGAGGATCGGTCCGGCGCGCGCGCGCCGTATC
ATGCTGCTGGCGCAGACGCTGGATGGACCCGATGCCGAGCGCCTGGGCCTCGTCGACGAACTCGCGACCGCGGGGCAGGT
CCAGGAACGGGCGCTGGAACTGGCGCAGCAGCTGGCGGCTGGGCCGCCCGCGGCCATGGCCAGCCTGCGGCGGGGCTTCT
ATGTCGCTGCGGCGGCGCTCGAGAGCGTGCTCGAGTACGAGGCCGCCGCCATGGCGATCGCCGTGACCGGCCACGAGTCC
CGCGAAGGCGTCGCGGCCTTCCTGGAGAAGCGCCCGCCGAGGTTCAGCTAG

Upstream 100 bases:

>100_bases
GCCTGGACCGGGTGCTGCTGTCCGAGGCGATCACCATGGGAATCTCGGCCCGGACCACCCGCGAGATGGGCTTCCTCCCC
AGCACCGTCCTGGCGAAGCG

Downstream 100 bases:

>100_bases
GGCCATGCGGTACGCGCTTCTCGGAGGGAGCGGGCTTGTCGTGTCTCGCCTGGCGTTCGGGGCGATGACCTTCGGCGGCC
CCTCACGCTACGGCCTCGGC

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSADVLLDRPAPRVARITLNTPETRNAASPGRTKLLRQLFDQALDDETVGAVVFTGAGGNFASGGNVKGFGTREPIEFLG
SVRQAQALLRRVLQGDKPVVAAVEGFAAGAGVGMACMCDVIVADANTRFVFPFTRIGMVPDLGLHYTLGQRIGPARARRI
MLLAQTLDGPDAERLGLVDELATAGQVQERALELAQQLAAGPPAAMASLRRGFYVAAAALESVLEYEAAAMAIAVTGHES
REGVAAFLEKRPPRFS

Sequences:

>Translated_256_residues
MSADVLLDRPAPRVARITLNTPETRNAASPGRTKLLRQLFDQALDDETVGAVVFTGAGGNFASGGNVKGFGTREPIEFLG
SVRQAQALLRRVLQGDKPVVAAVEGFAAGAGVGMACMCDVIVADANTRFVFPFTRIGMVPDLGLHYTLGQRIGPARARRI
MLLAQTLDGPDAERLGLVDELATAGQVQERALELAQQLAAGPPAAMASLRRGFYVAAAALESVLEYEAAAMAIAVTGHES
REGVAAFLEKRPPRFS
>Mature_255_residues
SADVLLDRPAPRVARITLNTPETRNAASPGRTKLLRQLFDQALDDETVGAVVFTGAGGNFASGGNVKGFGTREPIEFLGS
VRQAQALLRRVLQGDKPVVAAVEGFAAGAGVGMACMCDVIVADANTRFVFPFTRIGMVPDLGLHYTLGQRIGPARARRIM
LLAQTLDGPDAERLGLVDELATAGQVQERALELAQQLAAGPPAAMASLRRGFYVAAAALESVLEYEAAAMAIAVTGHESR
EGVAAFLEKRPPRFS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=22.1774193548387, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502327, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260274832, Length=198, Percent_Identity=25.7575757575758, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI45643119, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI157694516, Length=249, Percent_Identity=26.5060240963855, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI213417737, Length=214, Percent_Identity=27.5700934579439, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=31.1740890688259, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.4046692607004, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=32.258064516129, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=28.6245353159851, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=25.1968503937008, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=27.2277227722772, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=31.4516129032258, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=30.6772908366534, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=194, Percent_Identity=27.8350515463918, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=267, Percent_Identity=27.3408239700375, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=23.3870967741935, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=27.0531400966184, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=29.0836653386454, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=29.0836653386454, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=28.6274509803922, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24654903, Length=195, Percent_Identity=27.6923076923077, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=23.6434108527132, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27014; Mature: 26883

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADVLLDRPAPRVARITLNTPETRNAASPGRTKLLRQLFDQALDDETVGAVVFTGAGGN
CCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCC
FASGGNVKGFGTREPIEFLGSVRQAQALLRRVLQGDKPVVAAVEGFAAGAGVGMACMCDV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHH
IVADANTRFVFPFTRIGMVPDLGLHYTLGQRIGPARARRIMLLAQTLDGPDAERLGLVDE
HEECCCCEEEECHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
LATAGQVQERALELAQQLAAGPPAAMASLRRGFYVAAAALESVLEYEAAAMAIAVTGHES
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
REGVAAFLEKRPPRFS
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SADVLLDRPAPRVARITLNTPETRNAASPGRTKLLRQLFDQALDDETVGAVVFTGAGGN
CCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCC
FASGGNVKGFGTREPIEFLGSVRQAQALLRRVLQGDKPVVAAVEGFAAGAGVGMACMCDV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCHHHHHHH
IVADANTRFVFPFTRIGMVPDLGLHYTLGQRIGPARARRIMLLAQTLDGPDAERLGLVDE
HEECCCCEEEECHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
LATAGQVQERALELAQQLAAGPPAAMASLRRGFYVAAAALESVLEYEAAAMAIAVTGHES
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
REGVAAFLEKRPPRFS
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA