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The map label for this gene is pgl [H]

Identifier: 197105922

GI number: 197105922

Start: 2815388

End: 2816800

Strand: Direct

Name: pgl [H]

Synonym: PHZ_c2461

Alternate gene names: 197105922

Gene position: 2815388-2816800 (Clockwise)

Preceding gene: 197105921

Following gene: 197105923

Centisome position: 70.45

GC content: 70.13

Gene sequence:

>1413_bases
ATGATCAGCCGCCGTCAGATGCTCATCTCCGGCGCGGCGGCAGCCTGCGCCCCCGCCGCCGCCGCGGCCGCGGACGATCC
CTGGGCCGAGGCCGAGGCCATCGTCGCCCGGATCAGGCCGCCGATCTTCCCCGACCGGGATTTCCCCATCACCGACTTCG
GCGCCGACGGCGAGGCCGTGGCCGATTCCAGCGAGGCCTTCGCCAGGGCCATCGCCGCCTGCCACGCGGCCGGCGGCGGC
CGCGTGGTCGTGCCGCCGGGCTGGTGGCTGACCGGGCCGATCCACCTGAAGAGCAACGTCAACCTGCACGTCCAGAAGGG
TGCGACGATCCTCTTCAAGACCGATCCCAAAGCCTACCTGCCGCTGGTCTTCACCCGCTGGGAGGGCGTCGAGCTGATGA
ACTACTCGCCGTTCGTCTACGCCTTCGAGCAGGAGAACATCGCCGTCACCGGCGAGGGCGTGCTCGACGGCCAGTGCTCG
CGCGAGCACTGGTGGACCTGGAAGGGGCCCTGGAAGCAGAACCAGCACGGCTGGGCCGAGGGCATGCCCAACCAGCGGCC
CGCCCGCGCGCGGCTGTTCCAGATGGCCGAAGACCGCACGCCGGTCGAGCAGCGCGTCTTCGGCGAGGGAAGCTACCTGC
GCCCGCCGTTCATCCAGCCCTACCGCTGCACGAACGTGCTGATCGAGGGCGTGTCGCTGCGCCGTTCGCCGTTCTGGCAG
GTGCATCCGGTGCTGTGCCGAAACGTCACGATCCGCAGGCTGGACATCAACAGCCACGGCCCGAACAATGACGGCATCGA
CCCCGAGAGCTGCGACCACGTCCTCATCGAGGACTGCTTCTTCTCGACCGGCGACGACTGCATCGCCCTCAATTCGGGCC
GCAACGAGGACGGCCGACGGGTCGGGGTCCCCTGCCAGAACGTGGTGATCCGCGGCTGCCGCATGGCCGACGGCCACGGA
GGGCTGACCATCGGCAGCCAGATCTCGGGCCACGTCCGCAACGTCTTCGCCGAGAACTGCCGGCTGGACAGCCCCGACCT
CGACCACGCCATCCGCTTCAAGAACAACGCCCTGCGCGGCGGGATCGTGGAGCGGGTCCGCTACCGCAACCTCGAGGTCG
GCCAGGTGCGCCGCGCGGTGGTCACGGTGGACTTCAACTACGAGGAGGGGGCGAACGGCCGCTTCAAGCCCGTGCTGCGC
GACGTGCTGATCGAGAACGTCCGCAGCGGCCGAAGCCGCCGGGCCGTCGACCTGCAGGGCCTGCCCGGCGCGCCGGCCCG
CGACATCCGCATCGTGGACTGCGACTTCCGGGGCGTCGCCGAGCCTTCCATCGTCCGCCACGTCGAGGGGCTGAAGCTCA
CGAACGTGCGGGTGAACGGCAAGGTCGTCCGCGACCTGACGGCGCCGGTCTGA

Upstream 100 bases:

>100_bases
CGTCAGTTCTACTTCGGCGCCCGCTACAGCTTCTAAGCGGGCCCGGACGGCCGGGCGGTCCTCCCCCCGCCCGGCCTCTC
CCTTTTCCAGGAGGTCCGGC

Downstream 100 bases:

>100_bases
TGGGGGGGTGTCCGATGCTGCGCCGGACGCTGCTGGCGGGCCTCGCGGCGCTTTCGCTCGCCCTCCCGGCCCAGGCCCTC
CCAGCCCAGGCCCTCCCAGG

Product: exo-poly-alpha-D-galacturonosidase

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MISRRQMLISGAAAACAPAAAAAADDPWAEAEAIVARIRPPIFPDRDFPITDFGADGEAVADSSEAFARAIAACHAAGGG
RVVVPPGWWLTGPIHLKSNVNLHVQKGATILFKTDPKAYLPLVFTRWEGVELMNYSPFVYAFEQENIAVTGEGVLDGQCS
REHWWTWKGPWKQNQHGWAEGMPNQRPARARLFQMAEDRTPVEQRVFGEGSYLRPPFIQPYRCTNVLIEGVSLRRSPFWQ
VHPVLCRNVTIRRLDINSHGPNNDGIDPESCDHVLIEDCFFSTGDDCIALNSGRNEDGRRVGVPCQNVVIRGCRMADGHG
GLTIGSQISGHVRNVFAENCRLDSPDLDHAIRFKNNALRGGIVERVRYRNLEVGQVRRAVVTVDFNYEEGANGRFKPVLR
DVLIENVRSGRSRRAVDLQGLPGAPARDIRIVDCDFRGVAEPSIVRHVEGLKLTNVRVNGKVVRDLTAPV

Sequences:

>Translated_470_residues
MISRRQMLISGAAAACAPAAAAAADDPWAEAEAIVARIRPPIFPDRDFPITDFGADGEAVADSSEAFARAIAACHAAGGG
RVVVPPGWWLTGPIHLKSNVNLHVQKGATILFKTDPKAYLPLVFTRWEGVELMNYSPFVYAFEQENIAVTGEGVLDGQCS
REHWWTWKGPWKQNQHGWAEGMPNQRPARARLFQMAEDRTPVEQRVFGEGSYLRPPFIQPYRCTNVLIEGVSLRRSPFWQ
VHPVLCRNVTIRRLDINSHGPNNDGIDPESCDHVLIEDCFFSTGDDCIALNSGRNEDGRRVGVPCQNVVIRGCRMADGHG
GLTIGSQISGHVRNVFAENCRLDSPDLDHAIRFKNNALRGGIVERVRYRNLEVGQVRRAVVTVDFNYEEGANGRFKPVLR
DVLIENVRSGRSRRAVDLQGLPGAPARDIRIVDCDFRGVAEPSIVRHVEGLKLTNVRVNGKVVRDLTAPV
>Mature_470_residues
MISRRQMLISGAAAACAPAAAAAADDPWAEAEAIVARIRPPIFPDRDFPITDFGADGEAVADSSEAFARAIAACHAAGGG
RVVVPPGWWLTGPIHLKSNVNLHVQKGATILFKTDPKAYLPLVFTRWEGVELMNYSPFVYAFEQENIAVTGEGVLDGQCS
REHWWTWKGPWKQNQHGWAEGMPNQRPARARLFQMAEDRTPVEQRVFGEGSYLRPPFIQPYRCTNVLIEGVSLRRSPFWQ
VHPVLCRNVTIRRLDINSHGPNNDGIDPESCDHVLIEDCFFSTGDDCIALNSGRNEDGRRVGVPCQNVVIRGCRMADGHG
GLTIGSQISGHVRNVFAENCRLDSPDLDHAIRFKNNALRGGIVERVRYRNLEVGQVRRAVVTVDFNYEEGANGRFKPVLR
DVLIENVRSGRSRRAVDLQGLPGAPARDIRIVDCDFRGVAEPSIVRHVEGLKLTNVRVNGKVVRDLTAPV

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 52018; Mature: 52018

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRRQMLISGAAAACAPAAAAAADDPWAEAEAIVARIRPPIFPDRDFPITDFGADGEAV
CCCCHHHHHHCCHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
ADSSEAFARAIAACHAAGGGRVVVPPGWWLTGPIHLKSNVNLHVQKGATILFKTDPKAYL
CCCHHHHHHHHHHHHCCCCCEEEECCCCEEECEEEEECCCEEEEECCCEEEEECCCCCEE
PLVFTRWEGVELMNYSPFVYAFEQENIAVTGEGVLDGQCSREHWWTWKGPWKQNQHGWAE
CEEEEECCCEEEECCCCEEEEEECCCEEEECCCEECCCCCCCCCEEECCCCCCCCCCHHC
GMPNQRPARARLFQMAEDRTPVEQRVFGEGSYLRPPFIQPYRCTNVLIEGVSLRRSPFWQ
CCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCE
VHPVLCRNVTIRRLDINSHGPNNDGIDPESCDHVLIEDCFFSTGDDCIALNSGRNEDGRR
ECHHHHCCEEEEEEECCCCCCCCCCCCHHHCCCCEEHHHHCCCCCCEEEECCCCCCCCCE
VGVPCQNVVIRGCRMADGHGGLTIGSQISGHVRNVFAENCRLDSPDLDHAIRFKNNALRG
ECCCHHHHHEECCEEECCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
GIVERVRYRNLEVGQVRRAVVTVDFNYEEGANGRFKPVLRDVLIENVRSGRSRRAVDLQG
HHHHHHHHCCCCHHCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
LPGAPARDIRIVDCDFRGVAEPSIVRHVEGLKLTNVRVNGKVVRDLTAPV
CCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEEEEECCEEEECCCCCC
>Mature Secondary Structure
MISRRQMLISGAAAACAPAAAAAADDPWAEAEAIVARIRPPIFPDRDFPITDFGADGEAV
CCCCHHHHHHCCHHHHCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
ADSSEAFARAIAACHAAGGGRVVVPPGWWLTGPIHLKSNVNLHVQKGATILFKTDPKAYL
CCCHHHHHHHHHHHHCCCCCEEEECCCCEEECEEEEECCCEEEEECCCEEEEECCCCCEE
PLVFTRWEGVELMNYSPFVYAFEQENIAVTGEGVLDGQCSREHWWTWKGPWKQNQHGWAE
CEEEEECCCEEEECCCCEEEEEECCCEEEECCCEECCCCCCCCCEEECCCCCCCCCCHHC
GMPNQRPARARLFQMAEDRTPVEQRVFGEGSYLRPPFIQPYRCTNVLIEGVSLRRSPFWQ
CCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCE
VHPVLCRNVTIRRLDINSHGPNNDGIDPESCDHVLIEDCFFSTGDDCIALNSGRNEDGRR
ECHHHHCCEEEEEEECCCCCCCCCCCCHHHCCCCEEHHHHCCCCCCEEEECCCCCCCCCE
VGVPCQNVVIRGCRMADGHGGLTIGSQISGHVRNVFAENCRLDSPDLDHAIRFKNNALRG
ECCCHHHHHEECCEEECCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
GIVERVRYRNLEVGQVRRAVVTVDFNYEEGANGRFKPVLRDVLIENVRSGRSRRAVDLQG
HHHHHHHHCCCCHHCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
LPGAPARDIRIVDCDFRGVAEPSIVRHVEGLKLTNVRVNGKVVRDLTAPV
CCCCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEEEEECCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]