Click here to switch to the map view.

The map label for this gene is mtlK [H]

Identifier: 197105919

GI number: 197105919

Start: 2810431

End: 2811867

Strand: Direct

Name: mtlK [H]

Synonym: PHZ_c2458

Alternate gene names: 197105919

Gene position: 2810431-2811867 (Clockwise)

Preceding gene: 197105918

Following gene: 197105921

Centisome position: 70.33

GC content: 74.39

Gene sequence:

>1437_bases
GTGAAGCGCCTCTCCGACGCGACGCTGGCCCAGGCCGGCGCGCCCGTGCTCACGCCGGCCTACGACCGAACGACCCTGCG
CACCGGCGTGGTCCACTTCGGCCCCGGCGCCTTCCACCGCGCGCACCAGGCCTTCTACTTCGACCGGATGCTGGCGGGCC
GGCCCGACTTCGGCGTCTGCGCCGTATCGCTGCGCACGCCCGATGTGCGCGACGCCCTGGCGCCGCAGGACGGGCTCTAC
ACCCTTGCCGAGAAGGAGGCCGATCCGACCTATCGGGTGATCGGATCGATCCGCGAGGTGCTCGTCGCCCCGGAGGATCC
CGAGGCCGTGTTCGCGCGGCTGGCCTCGCCTGACGTGCGGTTCGTCACCTCCACCGTGACCGAGAAGGGCTATTGCCTGA
CGACCGCCGGCGACCTCGACCTGGACCACCCCGACGTCCGCCGCGACCTCCAGAGGCCGCAGGCGCCCGCCAGCCTGCCC
GGCTGGATCGTCGAGGCGCTGCGCCGGCGCCGGGCGGCGGGCCTTGCGCCCTTCGCGGCCATCAGCTGCGACAACCTCTC
GGACAACGGGGTGAAGCTGCGGCGGGCCGTGCTGCAGCTGGCCGAGGCGCGCGGCGAGGCCGACCTCGCCCGCTGGATCG
AGGCCGAGGCGGCCTTCCCCTGCAGCATGGTGGATTCCATCACTCCGGCCACGGACGAGGCCCTGCGCGCGCAGGTGGAC
GCCGCCCTCGGCCTGCACGACGCCTGGCCCGTGCAGCGCGAGCGCTTCACCCAGTGGGTCCTCGAGGACCGGCTCGGCGC
CGACGCCCAGGCCTTCGCCGACGCCGGCGTGACGGTGGCCAGGGCGGTGGCCCCGTTCGAACGGGCGAAGCTTCGGCTGC
TCAACGGCGCCCACTCCACCTTGGCCTACGTGGGCCTGGGGCTCGGCTACGAGACGGTCTTCGAGGCGATGGGCGATCCG
CGCCTGGCCCGCTTCGTCGAGCGGATGATGCGCGAGGACGTGGCCCCGACCCTGGACGCCCCCGAACTGGACGTGCCCGC
CTACATCGGCCAGGTGCTCGCCCGCTTCCGCAACCCGGCCATCGTCCACCGGCTGTCGCAGATCGCCTGGGACGGCTCGC
AGAAGCTGCGCTTCCGGCTGCTGGAGACCGCGTCCGAGGCGCTGGCCGCGGGCCGTCCGGCCGACCGGCTGGCGGTGGGC
GTGGCCGCGTGGATCCGGTTCGTAATGCGCCAGTCGGCGGCGGGCGTCCCGATCGTCGATCCGCTGGCCCCCCTGCTGGT
GGAGACCGCGCAAGCCGCCGGCCCGGCCGTCGATCGCTTCCTGGCTCTGGACGGCGTGTTTCCGCCGGCGCTGGCCGCCG
ACCCCCGATTCCGCGCGGCGGTGGCGGGAGCCTACGAGGCCCTGGCGAACGACCCGGCGTCGGCGCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCTGATGGACCTGGTGCTGGCCACCGCGAGCGGCCGGCCGACCCAGGCCGAGGAGAACGGCGAACGCGAGATCGCCCTC
TGGAAACGCGGGGTCACCCT

Downstream 100 bases:

>100_bases
GCCTGGCGGCGCAGCCGCTCCTCGATCCGCCGCACCAGCACCTCGGCCGGCAGGCCCATGCAGTCGGCCATGCGGCAGAA
CTCGAGCAGGTCCACGCGCC

Product: mannitol 2-dehydrogenase

Products: NA

Alternate protein names: Fructuronate reductase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKRLSDATLAQAGAPVLTPAYDRTTLRTGVVHFGPGAFHRAHQAFYFDRMLAGRPDFGVCAVSLRTPDVRDALAPQDGLY
TLAEKEADPTYRVIGSIREVLVAPEDPEAVFARLASPDVRFVTSTVTEKGYCLTTAGDLDLDHPDVRRDLQRPQAPASLP
GWIVEALRRRRAAGLAPFAAISCDNLSDNGVKLRRAVLQLAEARGEADLARWIEAEAAFPCSMVDSITPATDEALRAQVD
AALGLHDAWPVQRERFTQWVLEDRLGADAQAFADAGVTVARAVAPFERAKLRLLNGAHSTLAYVGLGLGYETVFEAMGDP
RLARFVERMMREDVAPTLDAPELDVPAYIGQVLARFRNPAIVHRLSQIAWDGSQKLRFRLLETASEALAAGRPADRLAVG
VAAWIRFVMRQSAAGVPIVDPLAPLLVETAQAAGPAVDRFLALDGVFPPALAADPRFRAAVAGAYEALANDPASALAA

Sequences:

>Translated_478_residues
MKRLSDATLAQAGAPVLTPAYDRTTLRTGVVHFGPGAFHRAHQAFYFDRMLAGRPDFGVCAVSLRTPDVRDALAPQDGLY
TLAEKEADPTYRVIGSIREVLVAPEDPEAVFARLASPDVRFVTSTVTEKGYCLTTAGDLDLDHPDVRRDLQRPQAPASLP
GWIVEALRRRRAAGLAPFAAISCDNLSDNGVKLRRAVLQLAEARGEADLARWIEAEAAFPCSMVDSITPATDEALRAQVD
AALGLHDAWPVQRERFTQWVLEDRLGADAQAFADAGVTVARAVAPFERAKLRLLNGAHSTLAYVGLGLGYETVFEAMGDP
RLARFVERMMREDVAPTLDAPELDVPAYIGQVLARFRNPAIVHRLSQIAWDGSQKLRFRLLETASEALAAGRPADRLAVG
VAAWIRFVMRQSAAGVPIVDPLAPLLVETAQAAGPAVDRFLALDGVFPPALAADPRFRAAVAGAYEALANDPASALAA
>Mature_478_residues
MKRLSDATLAQAGAPVLTPAYDRTTLRTGVVHFGPGAFHRAHQAFYFDRMLAGRPDFGVCAVSLRTPDVRDALAPQDGLY
TLAEKEADPTYRVIGSIREVLVAPEDPEAVFARLASPDVRFVTSTVTEKGYCLTTAGDLDLDHPDVRRDLQRPQAPASLP
GWIVEALRRRRAAGLAPFAAISCDNLSDNGVKLRRAVLQLAEARGEADLARWIEAEAAFPCSMVDSITPATDEALRAQVD
AALGLHDAWPVQRERFTQWVLEDRLGADAQAFADAGVTVARAVAPFERAKLRLLNGAHSTLAYVGLGLGYETVFEAMGDP
RLARFVERMMREDVAPTLDAPELDVPAYIGQVLARFRNPAIVHRLSQIAWDGSQKLRFRLLETASEALAAGRPADRLAVG
VAAWIRFVMRQSAAGVPIVDPLAPLLVETAQAAGPAVDRFLALDGVFPPALAADPRFRAAVAGAYEALANDPASALAA

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=474, Percent_Identity=40.9282700421941, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1788497, Length=475, Percent_Identity=41.2631578947368, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1787823, Length=470, Percent_Identity=40, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI48994885, Length=465, Percent_Identity=30.9677419354839, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790028, Length=267, Percent_Identity=26.9662921348315, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324401, Length=479, Percent_Identity=36.1169102296451, Blast_Score=299, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6320765, Length=479, Percent_Identity=36.1169102296451, Blast_Score=299, Evalue=6e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.57 [H]

Molecular weight: Translated: 51481; Mature: 51481

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSDATLAQAGAPVLTPAYDRTTLRTGVVHFGPGAFHRAHQAFYFDRMLAGRPDFGVC
CCCCCCHHHHHCCCCCCCCCCCCHHHHHCCEEECCHHHHHHHHHHHHHHHHCCCCCCCEE
AVSLRTPDVRDALAPQDGLYTLAEKEADPTYRVIGSIREVLVAPEDPEAVFARLASPDVR
EEEECCCCHHHHCCCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHH
FVTSTVTEKGYCLTTAGDLDLDHPDVRRDLQRPQAPASLPGWIVEALRRRRAAGLAPFAA
HHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHE
ISCDNLSDNGVKLRRAVLQLAEARGEADLARWIEAEAAFPCSMVDSITPATDEALRAQVD
EECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHH
AALGLHDAWPVQRERFTQWVLEDRLGADAQAFADAGVTVARAVAPFERAKLRLLNGAHST
HHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHH
LAYVGLGLGYETVFEAMGDPRLARFVERMMREDVAPTLDAPELDVPAYIGQVLARFRNPA
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHH
IVHRLSQIAWDGSQKLRFRLLETASEALAAGRPADRLAVGVAAWIRFVMRQSAAGVPIVD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
PLAPLLVETAQAAGPAVDRFLALDGVFPPALAADPRFRAAVAGAYEALANDPASALAA
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MKRLSDATLAQAGAPVLTPAYDRTTLRTGVVHFGPGAFHRAHQAFYFDRMLAGRPDFGVC
CCCCCCHHHHHCCCCCCCCCCCCHHHHHCCEEECCHHHHHHHHHHHHHHHHCCCCCCCEE
AVSLRTPDVRDALAPQDGLYTLAEKEADPTYRVIGSIREVLVAPEDPEAVFARLASPDVR
EEEECCCCHHHHCCCCCCCEEHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHH
FVTSTVTEKGYCLTTAGDLDLDHPDVRRDLQRPQAPASLPGWIVEALRRRRAAGLAPFAA
HHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHE
ISCDNLSDNGVKLRRAVLQLAEARGEADLARWIEAEAAFPCSMVDSITPATDEALRAQVD
EECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHH
AALGLHDAWPVQRERFTQWVLEDRLGADAQAFADAGVTVARAVAPFERAKLRLLNGAHST
HHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHH
LAYVGLGLGYETVFEAMGDPRLARFVERMMREDVAPTLDAPELDVPAYIGQVLARFRNPA
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHH
IVHRLSQIAWDGSQKLRFRLLETASEALAAGRPADRLAVGVAAWIRFVMRQSAAGVPIVD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
PLAPLLVETAQAAGPAVDRFLALDGVFPPALAADPRFRAAVAGAYEALANDPASALAA
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]