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The map label for this gene is vsr [H]

Identifier: 197105893

GI number: 197105893

Start: 2777114

End: 2777608

Strand: Direct

Name: vsr [H]

Synonym: PHZ_c2432

Alternate gene names: 197105893

Gene position: 2777114-2777608 (Clockwise)

Preceding gene: 197105892

Following gene: 197105901

Centisome position: 69.49

GC content: 67.68

Gene sequence:

>495_bases
TTGTCGCCTCGGCAGGCCAGTCGCGCAGATCCGCTGACGCCCCAGCAGCGACGGCGGAATATGGCGGCCATCCGCGCGGC
GAACACGAAGCCAGAGATGCTCGTCAGGCGCGGCCTCCACGCCCATGGCCTTCGCTACCGCCTGCACGATCGCGGACTGC
CCGGGACGCCGGACCTCGTCTTCCGGAGCGCCCGCGTCGCCGTCTTCGTGCACGGCTGCTTCTGGCATGGTCACGACTGT
TCACTCGGCGTCAGACCCCGTACGAACGCCGACTTCTGGACCGCCAAGATCAACCGCAATCGCGAAAGAGACGAAGCCGC
TGAGGCGGCACTTCTGATGTTGGGCTGGCGGGTCGCCACTGTCTGGGAATGTGCGCTTCGCGGCCGTGAGCGGCCCCCGC
TGCCTGAGACGCTCGGCAGGCTTGCAGCCTTCATCCGGAGCGGGTCCCCATCAGCGCTCGTCCTTCGAAGCCCAGATCGC
GACCGTAACGCGTGA

Upstream 100 bases:

>100_bases
CCTCCCCTTCTGGCCCGGGCGGTCGCCGGCGAGATTGCAGCGGCGTTCCAGCGGGCGATGGCGCCTGATCGTCCGCGTAC
GGGCCGGCTGGCCGCCTGAG

Downstream 100 bases:

>100_bases
TTGGCGGCGCGGAGCGACGACCGCAGAGGCGGCCGGGCCAACAGGCGAAGTCACGCTGCCCGGAGGCCCGCCGTCCGGCC
AGACATTCAGATATCGGCGT

Product: patch repair protein

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MSPRQASRADPLTPQQRRRNMAAIRAANTKPEMLVRRGLHAHGLRYRLHDRGLPGTPDLVFRSARVAVFVHGCFWHGHDC
SLGVRPRTNADFWTAKINRNRERDEAAEAALLMLGWRVATVWECALRGRERPPLPETLGRLAAFIRSGSPSALVLRSPDR
DRNA

Sequences:

>Translated_164_residues
MSPRQASRADPLTPQQRRRNMAAIRAANTKPEMLVRRGLHAHGLRYRLHDRGLPGTPDLVFRSARVAVFVHGCFWHGHDC
SLGVRPRTNADFWTAKINRNRERDEAAEAALLMLGWRVATVWECALRGRERPPLPETLGRLAAFIRSGSPSALVLRSPDR
DRNA
>Mature_163_residues
SPRQASRADPLTPQQRRRNMAAIRAANTKPEMLVRRGLHAHGLRYRLHDRGLPGTPDLVFRSARVAVFVHGCFWHGHDCS
LGVRPRTNADFWTAKINRNRERDEAAEAALLMLGWRVATVWECALRGRERPPLPETLGRLAAFIRSGSPSALVLRSPDRD
RNA

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=146, Percent_Identity=42.4657534246575, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 18532; Mature: 18400

Theoretical pI: Translated: 12.05; Mature: 12.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRQASRADPLTPQQRRRNMAAIRAANTKPEMLVRRGLHAHGLRYRLHDRGLPGTPDLV
CCCCCCCCCCCCCHHHHHCCHHHEEECCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHHH
FRSARVAVFVHGCFWHGHDCSLGVRPRTNADFWTAKINRNRERDEAAEAALLMLGWRVAT
HHCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VWECALRGRERPPLPETLGRLAAFIRSGSPSALVLRSPDRDRNA
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SPRQASRADPLTPQQRRRNMAAIRAANTKPEMLVRRGLHAHGLRYRLHDRGLPGTPDLV
CCCCCCCCCCCCHHHHHCCHHHEEECCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHHH
FRSARVAVFVHGCFWHGHDCSLGVRPRTNADFWTAKINRNRERDEAAEAALLMLGWRVAT
HHCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
VWECALRGRERPPLPETLGRLAAFIRSGSPSALVLRSPDRDRNA
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]