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The map label for this gene is citE [H]

Identifier: 197105865

GI number: 197105865

Start: 2737496

End: 2738098

Strand: Reverse

Name: citE [H]

Synonym: PHZ_c2404

Alternate gene names: 197105865

Gene position: 2738098-2737496 (Counterclockwise)

Preceding gene: 197105866

Following gene: 197105864

Centisome position: 68.52

GC content: 70.98

Gene sequence:

>603_bases
GTGGTCCTGCCGAAGGCCAGCCCACAGGCGGTCCGGGACCTCGAGGTGGCGCTCACGCAGCAGGAGCGAACCTGGCGGCG
TCAGGAAGAGCCGGTGCGGGTGTTGCCGATCGCCACGGAGACGGCTGCGGCCGTCTTCACGCTGGGGGCCTACTCCACCT
GCCGCGGGCGGCTCGACGGGCTGACCTGGGGCGCCGAGGACCTGTCCGCCGCCATCGGGGCGGAGACCCCGCGCGACAGC
GAGAAGCAGCTTACGCCCGTCTATGAGGTGGTGCGGACGATGGCCCTGCTGGGCGCGGCCGCGGCGGACATCCCGGCGAT
CGAGACGGTGTACCCTGACTTCTCGGACCTGGCGGGACTGGCCGCCTACGCTGAACGGGGCCGCCGCGATGGGTTCGTCG
GGATGATGGCGATCCATCCAGCTCAGGTCCCGGTCATCAACTCCGCCTTTGCGCCGTCGCAGGACGAGATCGCCGGCGCC
CAGAGGATCATCGAGGCCTTCGCGAAGGCTGAGGCCGGGGTGGTGGCGCTCGATGGCCGCATGCTGGACGCGCCGCATCT
CGTGCAGGCCCGTCGAATCCTCAGTCGGGCCGGCAGAGCATGA

Upstream 100 bases:

>100_bases
CAGCCCGGATCGCGCGCGGCTCTGGGTTCGCATCAATCCGCTGTCGACGGCTGAGGGGGGAGAGGACCTCGCCGCCGCGA
CCGCAGCCGGCGCCGTCGGC

Downstream 100 bases:

>100_bases
TCTGGCGCCACAGCGCCAGCGCAGCCGTTGCGCTGCGCGGCCGCATTCGACGCTGGCGGCACAGAGCTGAAGCGGCTCGC
CGCCACCCTGGATGGCTGCC

Product: citrate lyase, beta subunit protein

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MVLPKASPQAVRDLEVALTQQERTWRRQEEPVRVLPIATETAAAVFTLGAYSTCRGRLDGLTWGAEDLSAAIGAETPRDS
EKQLTPVYEVVRTMALLGAAAADIPAIETVYPDFSDLAGLAAYAERGRRDGFVGMMAIHPAQVPVINSAFAPSQDEIAGA
QRIIEAFAKAEAGVVALDGRMLDAPHLVQARRILSRAGRA

Sequences:

>Translated_200_residues
MVLPKASPQAVRDLEVALTQQERTWRRQEEPVRVLPIATETAAAVFTLGAYSTCRGRLDGLTWGAEDLSAAIGAETPRDS
EKQLTPVYEVVRTMALLGAAAADIPAIETVYPDFSDLAGLAAYAERGRRDGFVGMMAIHPAQVPVINSAFAPSQDEIAGA
QRIIEAFAKAEAGVVALDGRMLDAPHLVQARRILSRAGRA
>Mature_200_residues
MVLPKASPQAVRDLEVALTQQERTWRRQEEPVRVLPIATETAAAVFTLGAYSTCRGRLDGLTWGAEDLSAAIGAETPRDS
EKQLTPVYEVVRTMALLGAAAADIPAIETVYPDFSDLAGLAAYAERGRRDGFVGMMAIHPAQVPVINSAFAPSQDEIAGA
QRIIEAFAKAEAGVVALDGRMLDAPHLVQARRILSRAGRA

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=185, Percent_Identity=31.8918918918919, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI87081764, Length=200, Percent_Identity=32, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17557458, Length=146, Percent_Identity=33.5616438356164, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 21394; Mature: 21394

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPKASPQAVRDLEVALTQQERTWRRQEEPVRVLPIATETAAAVFTLGAYSTCRGRLDG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
LTWGAEDLSAAIGAETPRDSEKQLTPVYEVVRTMALLGAAAADIPAIETVYPDFSDLAGL
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHH
AAYAERGRRDGFVGMMAIHPAQVPVINSAFAPSQDEIAGAQRIIEAFAKAEAGVVALDGR
HHHHHCCCCCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCE
MLDAPHLVQARRILSRAGRA
ECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVLPKASPQAVRDLEVALTQQERTWRRQEEPVRVLPIATETAAAVFTLGAYSTCRGRLDG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
LTWGAEDLSAAIGAETPRDSEKQLTPVYEVVRTMALLGAAAADIPAIETVYPDFSDLAGL
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHH
AAYAERGRRDGFVGMMAIHPAQVPVINSAFAPSQDEIAGAQRIIEAFAKAEAGVVALDGR
HHHHHCCCCCCCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCE
MLDAPHLVQARRILSRAGRA
ECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]