Click here to switch to the map view.

The map label for this gene is ooxB [H]

Identifier: 197105835

GI number: 197105835

Start: 2691607

End: 2692788

Strand: Reverse

Name: ooxB [H]

Synonym: PHZ_c2374

Alternate gene names: 197105835

Gene position: 2692788-2691607 (Counterclockwise)

Preceding gene: 197105836

Following gene: 197105834

Centisome position: 67.38

GC content: 69.54

Gene sequence:

>1182_bases
ATGAGCGCCAAGGCCGACGTGCTGGTCATCGGCGGCGGCCTTCATGGCCTGTCGGCCGCCCTGCAGATCGCCCGCCGCGG
GGCCCGGGTGACCCTGCTGGAACGCCGCCGCGTCGGCCGGCATTCATCCGGCGCCTCGGCCGCCGGCGTTCGCACGCTTG
GCCGGGCGCGCGCCGAACTGCCGCTTTCGCTGGCCGCGATGACCATGTGGCACGGAATCGCCGAGATCGTCGGGGATGAC
TGCGGCTTCCATGCGCACGGCCAGCTTCAGGTAGCTGAGACGGAGGCTGAGCTCGAGCGGATCAGGGCCCGTGTGGACCA
CCTCCGCAGAGACGGCTTTTACAACGAGGAGCTGGTGGACGCCGCGCAGCTCCGCGAACTCGTGCCTGGCCTGGCCCGCC
ACTGCATCGGTGGCGCCTTCGCCAAGGACGATGGCGCGGCCGATCCTCACCGGACGCTGGCCGCCTATCTTCGTGCAGCA
ACCGAGGCGGGCGTCGTCGTCTGCGAGGGAACGGGCGTCACACAGCTGGAGCGACGCGATGGCGTCTGGCGCATTGGCGC
CGGGCCGGCGTGGTTTGAAGCGCCGCTGGTCGTAAACGCAGCCGGGGCCTGGGCGGGAGAAATCGCCGCGATGGCCGGTG
ACCACATCCCGCTCGACACCAAGGCCTCGATGATGATCGTCACCGAGCGCATCGCACCTTTTGTCCGCCCGACTGTGAGC
ACCGCGGGCCGCAAGCTCTCCTTCAAGCAGACCGACCAGGGCACGCTTCTGATCGGCGGCGGCCAGCAGGGGCGGGCCGA
TCTGGCGAACGAGACAGCCGAGGTCGACATCCGCACCCTGGCCAGATCCGCCGCGGCGGCGGTCGCTCTCTTCCCGAGCG
TCGCCGGTCTGAGGATCGTCCGGAGCTGGGCGGGGCTCGAAGCCAGGACCAGCGATGATATTGCGATCGCCGGATTGTCG
CCGACGGCGCCGGGTCTGCTGCACCTGTTCGGGTTTTGTGGGCACGGCTTTCAGCTGGTGCCGGCTCTGGGCGTGGCTGC
GGCCGATCTGATGCTGACGGGACAGACGGCGTTCGACCTGAACGGACTCCAACCCGGTCGGCTGATGACAGCGGGAAAAC
TTAACAGGCGCTCGACGCGGGACGGCGAAGCGCGCGCGCCGCTCGCCGCCTCCGACGATTAG

Upstream 100 bases:

>100_bases
AGACCCATCAGCTGCCATTTGGGGCCGTCGGGTTCCTGAAGATCCGCCCGCCATTGAAGCCGCTGACCCTGAAGGAGCTC
GCCTCCCTGGAGACTGACGC

Downstream 100 bases:

>100_bases
CCATTCCGCCAGAGAGCGCAGGACCGGAGACGTACATGAGCGACATCGAACGGATCGCGACGAATCCTCGGCGGGGCCGC
GTCGTGATTCACAATGGGCT

Product: FAD dependent oxidoreductase

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSAKADVLVIGGGLHGLSAALQIARRGARVTLLERRRVGRHSSGASAAGVRTLGRARAELPLSLAAMTMWHGIAEIVGDD
CGFHAHGQLQVAETEAELERIRARVDHLRRDGFYNEELVDAAQLRELVPGLARHCIGGAFAKDDGAADPHRTLAAYLRAA
TEAGVVVCEGTGVTQLERRDGVWRIGAGPAWFEAPLVVNAAGAWAGEIAAMAGDHIPLDTKASMMIVTERIAPFVRPTVS
TAGRKLSFKQTDQGTLLIGGGQQGRADLANETAEVDIRTLARSAAAAVALFPSVAGLRIVRSWAGLEARTSDDIAIAGLS
PTAPGLLHLFGFCGHGFQLVPALGVAAADLMLTGQTAFDLNGLQPGRLMTAGKLNRRSTRDGEARAPLAASDD

Sequences:

>Translated_393_residues
MSAKADVLVIGGGLHGLSAALQIARRGARVTLLERRRVGRHSSGASAAGVRTLGRARAELPLSLAAMTMWHGIAEIVGDD
CGFHAHGQLQVAETEAELERIRARVDHLRRDGFYNEELVDAAQLRELVPGLARHCIGGAFAKDDGAADPHRTLAAYLRAA
TEAGVVVCEGTGVTQLERRDGVWRIGAGPAWFEAPLVVNAAGAWAGEIAAMAGDHIPLDTKASMMIVTERIAPFVRPTVS
TAGRKLSFKQTDQGTLLIGGGQQGRADLANETAEVDIRTLARSAAAAVALFPSVAGLRIVRSWAGLEARTSDDIAIAGLS
PTAPGLLHLFGFCGHGFQLVPALGVAAADLMLTGQTAFDLNGLQPGRLMTAGKLNRRSTRDGEARAPLAASDD
>Mature_392_residues
SAKADVLVIGGGLHGLSAALQIARRGARVTLLERRRVGRHSSGASAAGVRTLGRARAELPLSLAAMTMWHGIAEIVGDDC
GFHAHGQLQVAETEAELERIRARVDHLRRDGFYNEELVDAAQLRELVPGLARHCIGGAFAKDDGAADPHRTLAAYLRAAT
EAGVVVCEGTGVTQLERRDGVWRIGAGPAWFEAPLVVNAAGAWAGEIAAMAGDHIPLDTKASMMIVTERIAPFVRPTVST
AGRKLSFKQTDQGTLLIGGGQQGRADLANETAEVDIRTLARSAAAAVALFPSVAGLRIVRSWAGLEARTSDDIAIAGLSP
TAPGLLHLFGFCGHGFQLVPALGVAAADLMLTGQTAFDLNGLQPGRLMTAGKLNRRSTRDGEARAPLAASDD

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=408, Percent_Identity=24.5098039215686, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI197927446, Length=390, Percent_Identity=24.8717948717949, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI21361378, Length=390, Percent_Identity=24.8717948717949, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32563613, Length=384, Percent_Identity=24.4791666666667, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130091, Length=375, Percent_Identity=27.4666666666667, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 41168; Mature: 41037

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKADVLVIGGGLHGLSAALQIARRGARVTLLERRRVGRHSSGASAAGVRTLGRARAEL
CCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHCC
PLSLAAMTMWHGIAEIVGDDCGFHAHGQLQVAETEAELERIRARVDHLRRDGFYNEELVD
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAQLRELVPGLARHCIGGAFAKDDGAADPHRTLAAYLRAATEAGVVVCEGTGVTQLERRD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEHHCC
GVWRIGAGPAWFEAPLVVNAAGAWAGEIAAMAGDHIPLDTKASMMIVTERIAPFVRPTVS
CEEEECCCCCCEECCEEEEECCCCHHHHHHHCCCCCCCCCCCHHEEEHHHHHHHHHHHHH
TAGRKLSFKQTDQGTLLIGGGQQGRADLANETAEVDIRTLARSAAAAVALFPSVAGLRIV
HCCCEEEEEECCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWAGLEARTSDDIAIAGLSPTAPGLLHLFGFCGHGFQLVPALGVAAADLMLTGQTAFDL
HHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCEECC
NGLQPGRLMTAGKLNRRSTRDGEARAPLAASDD
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAKADVLVIGGGLHGLSAALQIARRGARVTLLERRRVGRHSSGASAAGVRTLGRARAEL
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHCC
PLSLAAMTMWHGIAEIVGDDCGFHAHGQLQVAETEAELERIRARVDHLRRDGFYNEELVD
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAQLRELVPGLARHCIGGAFAKDDGAADPHRTLAAYLRAATEAGVVVCEGTGVTQLERRD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEHHCC
GVWRIGAGPAWFEAPLVVNAAGAWAGEIAAMAGDHIPLDTKASMMIVTERIAPFVRPTVS
CEEEECCCCCCEECCEEEEECCCCHHHHHHHCCCCCCCCCCCHHEEEHHHHHHHHHHHHH
TAGRKLSFKQTDQGTLLIGGGQQGRADLANETAEVDIRTLARSAAAAVALFPSVAGLRIV
HCCCEEEEEECCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSWAGLEARTSDDIAIAGLSPTAPGLLHLFGFCGHGFQLVPALGVAAADLMLTGQTAFDL
HHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEEECCCEECC
NGLQPGRLMTAGKLNRRSTRDGEARAPLAASDD
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]