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The map label for this gene is virB4 [H]

Identifier: 197105812

GI number: 197105812

Start: 2661119

End: 2663500

Strand: Direct

Name: virB4 [H]

Synonym: PHZ_c2350

Alternate gene names: 197105812

Gene position: 2661119-2663500 (Clockwise)

Preceding gene: 197105811

Following gene: 197105813

Centisome position: 66.59

GC content: 68.05

Gene sequence:

>2382_bases
TTGCGACCGCGGAGGTTACCCGTGGCTAGCCTCTCCGCCCGCCGGCGCGAACCAGAACCGGCTCGCTTCCTGCCCTATGC
CCTCCATGTCTCCGACAGCGTGCTGGCGCTGGATAGCGGGGCCTTGATGACCTGCTTCCGCCTCACTGGGGCGAGTTTCG
AAACGGCCGACATTCGGGACCTCAACGACCGACACGTCAGGCTCAACGCCAGCTGGCGAAATCTCGCCAGCGACCGGCTC
GCCGTCTGGCATCATCTCGTGCGGCGTCAGCAGACCGCGTGGCCGGACGGCGATTTCCAGTCGGCCTTCGCGCAAGAACT
GGACGCGGCTTATCGCGGCCGCATGGCCGCAGGGCGGCTCTACGCGAATGACCTCTTCGTCACCCTCGTTCTGCACCCCG
GTGTTGACCCTGCGGAGCGCACCCAGGCGTGGGTGCAGCGTCTGATCGGCCGCGGCGTCCCCGCCGTCGATGACGCCGAT
GTCTCGCGGCTCGAGGAGATCGCCGAGGACCTGGCGCAACACCTCAGGCGCTATGAACCGACCCGGCTTGGTCTGCGTCA
CCTCGACGGTCTCGTCTTTTCCGAGCCGCTCGAGATGCTGCGCCTGATCCTCACCGGTCGTCCTGAGGCCGTCCCCCTCA
CGCGAGGTCACCTGGGAGCTGCGCTCTACGCCTCACGACTGATCTTTGGCCGTGAGGCGATCGAGATCCGCGACGCCGCC
GAGACGCGCTATGCCGGCATGCTGGGGTTGAAGGAGTATCCGGCCGCAACGCGGCCCGGGCACTGGGACACCCTGCTCTC
CGCCCCCTTTCCCTTTGTGCTCTCCCAGTCTTTCGCCTTCCTCTCGAAGCCGGCCGCCCAGGCGGTGATGAGCCGCAAGC
AGAACCAGATGGTCTCGACGCGGGACCGGGCGGCCTCTCAGATCGCGGGACTCGACGAGGCCCTGGACGACCTGACCTCG
AACCGCTTCGCGCTTGGAGAACACCAGGCCTCACTTCTGGTCCTGGGCGACACCGCTCGCGCTTTGGCTGACCACATGTC
GCGCGCCAGCGCGCTCCTGGCCGAAAGCGGCCTTGTCGTCGTGCGCGAGGATCTCGGGCTGGAGGCCGGCTTCTGGGCGC
AGTTCCCGGGCAACTTCCGCTATCGGCTGCGGCCCGCGGCGATCACCAGCCGCAATTTCGCAGGCCTGGCGCCCTTCCAC
ACCCATCCCGCGGGACGCGGTCATGGCAATCACTGGGGCGAGGCCGTCGCCCTGCTGCGCACCACGGCTGGGTCGCCCTA
CCACTTCAATTTCCACGCCGGCGACCTCGGCCACACCTTCATCTGCGGCCCCTCAGGCTCGGGGAAGACGGTCGTCCAGA
ACTTCATGCTCGGCCAGCTGGAGAAGCTCGGCTGCCAGCAGGTGTTCATCGACAAGGATCGGGGGGCGGAAATCTTCATC
CGCGCCTGCGGTGGGACGTACCTCACACTGCGCCCCGGCGAGCCCACCGGCTTTGCGCCGCTGAAGGCGGCCGCGGATAC
GCCGCCAGACCGGGCGTTTCTCCGCGCATTGATCCGCAGCCTCGTCGCGGGCGCGGATGGCGCATTGGGCGTCGAGGACG
AGAACGCCATCGAGCGGGCGGTCGCCGCCGTCCTGAAGCTGCCCGCCGACGAGCGCTCGTTCGCGGCGCTCCGCAGTCTC
CTCGGTCAGCGGGAAGCCGGCGGGATTGGCGCGCGCCTGGATCGGTGGCGGCAGGGACGGGCGCTGGGTTGGGCGCTCGA
CGGCGAAACCGATGCGCTGGATCTGTCTCCACGTCTCGCCGGCTTCGACATGACCCATCTCCTCGACTCCGCGGAGGTGC
GAACTCCGTTGATGCTCTACCTCTTCCACCGGCTGCAGAAGCTGGTCGACGGCCGACGCCTCGTCATCGACATCGACGAG
TTCTGGAAGGCGCTCGGCGACGAGGCCTTCCGGGGGCTGGCTGAGGACGGCCTGAAGACCTTCCGCAAGCAGAACGCCTT
CATGGTGTTCGGCACCCAGTCCCCCGCTGACGTCCTGCGCTCGCCGATCGCCCACACGATCGTTGAGCAGTGCGCCACCA
AAATCTTCCTGCCCAATCCCCACGCCAGCGAGCGGGATTACGTCGACGGCTTCGGTCTCACATGCCGCGAGTTCCAGCTC
ATCCGCGACGAGCTCAGCGCCGAACAGCGCCGCTTCCTGGTGAAGCAGGGGCTCGACTCCGTCGTCGTCGAGCTCGACCT
GGGCGGCCTCGAGGACGCCCTGGCGGTGCTGTCAGGCCGCGCAGAGACGGTCGACCTGCTCGATCGCATTCGCGGGATCC
ACGGCGACGATCCCACTGCCTGGCTCGCCCCCTTCCAGCAAGCCCGACGCGAGGCGCGATGA

Upstream 100 bases:

>100_bases
TTCCGGGTCCTCTACCTGTTCATTGAAACCAAGGGCCGGGCGCGCAATGGCCGGCTCTGGGGCGGCAGCTCGCCGACTCC
TCTGCCGCTGCGCCGCCGCT

Downstream 100 bases:

>100_bases
AACCTCTTTGCCTTCCCCTGATGCTGGGGGCGATTTCAGCCGCCACGCCCGCCGCCGCCCAGCTCGTGGTGCACGATCCC
ACGAACTACGGGAGCCTGCT

Product: type IV secretory pathway, VirB4 component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MRPRRLPVASLSARRREPEPARFLPYALHVSDSVLALDSGALMTCFRLTGASFETADIRDLNDRHVRLNASWRNLASDRL
AVWHHLVRRQQTAWPDGDFQSAFAQELDAAYRGRMAAGRLYANDLFVTLVLHPGVDPAERTQAWVQRLIGRGVPAVDDAD
VSRLEEIAEDLAQHLRRYEPTRLGLRHLDGLVFSEPLEMLRLILTGRPEAVPLTRGHLGAALYASRLIFGREAIEIRDAA
ETRYAGMLGLKEYPAATRPGHWDTLLSAPFPFVLSQSFAFLSKPAAQAVMSRKQNQMVSTRDRAASQIAGLDEALDDLTS
NRFALGEHQASLLVLGDTARALADHMSRASALLAESGLVVVREDLGLEAGFWAQFPGNFRYRLRPAAITSRNFAGLAPFH
THPAGRGHGNHWGEAVALLRTTAGSPYHFNFHAGDLGHTFICGPSGSGKTVVQNFMLGQLEKLGCQQVFIDKDRGAEIFI
RACGGTYLTLRPGEPTGFAPLKAAADTPPDRAFLRALIRSLVAGADGALGVEDENAIERAVAAVLKLPADERSFAALRSL
LGQREAGGIGARLDRWRQGRALGWALDGETDALDLSPRLAGFDMTHLLDSAEVRTPLMLYLFHRLQKLVDGRRLVIDIDE
FWKALGDEAFRGLAEDGLKTFRKQNAFMVFGTQSPADVLRSPIAHTIVEQCATKIFLPNPHASERDYVDGFGLTCREFQL
IRDELSAEQRRFLVKQGLDSVVVELDLGGLEDALAVLSGRAETVDLLDRIRGIHGDDPTAWLAPFQQARREAR

Sequences:

>Translated_793_residues
MRPRRLPVASLSARRREPEPARFLPYALHVSDSVLALDSGALMTCFRLTGASFETADIRDLNDRHVRLNASWRNLASDRL
AVWHHLVRRQQTAWPDGDFQSAFAQELDAAYRGRMAAGRLYANDLFVTLVLHPGVDPAERTQAWVQRLIGRGVPAVDDAD
VSRLEEIAEDLAQHLRRYEPTRLGLRHLDGLVFSEPLEMLRLILTGRPEAVPLTRGHLGAALYASRLIFGREAIEIRDAA
ETRYAGMLGLKEYPAATRPGHWDTLLSAPFPFVLSQSFAFLSKPAAQAVMSRKQNQMVSTRDRAASQIAGLDEALDDLTS
NRFALGEHQASLLVLGDTARALADHMSRASALLAESGLVVVREDLGLEAGFWAQFPGNFRYRLRPAAITSRNFAGLAPFH
THPAGRGHGNHWGEAVALLRTTAGSPYHFNFHAGDLGHTFICGPSGSGKTVVQNFMLGQLEKLGCQQVFIDKDRGAEIFI
RACGGTYLTLRPGEPTGFAPLKAAADTPPDRAFLRALIRSLVAGADGALGVEDENAIERAVAAVLKLPADERSFAALRSL
LGQREAGGIGARLDRWRQGRALGWALDGETDALDLSPRLAGFDMTHLLDSAEVRTPLMLYLFHRLQKLVDGRRLVIDIDE
FWKALGDEAFRGLAEDGLKTFRKQNAFMVFGTQSPADVLRSPIAHTIVEQCATKIFLPNPHASERDYVDGFGLTCREFQL
IRDELSAEQRRFLVKQGLDSVVVELDLGGLEDALAVLSGRAETVDLLDRIRGIHGDDPTAWLAPFQQARREAR
>Mature_793_residues
MRPRRLPVASLSARRREPEPARFLPYALHVSDSVLALDSGALMTCFRLTGASFETADIRDLNDRHVRLNASWRNLASDRL
AVWHHLVRRQQTAWPDGDFQSAFAQELDAAYRGRMAAGRLYANDLFVTLVLHPGVDPAERTQAWVQRLIGRGVPAVDDAD
VSRLEEIAEDLAQHLRRYEPTRLGLRHLDGLVFSEPLEMLRLILTGRPEAVPLTRGHLGAALYASRLIFGREAIEIRDAA
ETRYAGMLGLKEYPAATRPGHWDTLLSAPFPFVLSQSFAFLSKPAAQAVMSRKQNQMVSTRDRAASQIAGLDEALDDLTS
NRFALGEHQASLLVLGDTARALADHMSRASALLAESGLVVVREDLGLEAGFWAQFPGNFRYRLRPAAITSRNFAGLAPFH
THPAGRGHGNHWGEAVALLRTTAGSPYHFNFHAGDLGHTFICGPSGSGKTVVQNFMLGQLEKLGCQQVFIDKDRGAEIFI
RACGGTYLTLRPGEPTGFAPLKAAADTPPDRAFLRALIRSLVAGADGALGVEDENAIERAVAAVLKLPADERSFAALRSL
LGQREAGGIGARLDRWRQGRALGWALDGETDALDLSPRLAGFDMTHLLDSAEVRTPLMLYLFHRLQKLVDGRRLVIDIDE
FWKALGDEAFRGLAEDGLKTFRKQNAFMVFGTQSPADVLRSPIAHTIVEQCATKIFLPNPHASERDYVDGFGLTCREFQL
IRDELSAEQRRFLVKQGLDSVVVELDLGGLEDALAVLSGRAETVDLLDRIRGIHGDDPTAWLAPFQQARREAR

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 87369; Mature: 87369

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRRLPVASLSARRREPEPARFLPYALHVSDSVLALDSGALMTCFRLTGASFETADIRD
CCCCCCCHHHHHHHCCCCCCHHHCCEEEECCCCEEEECCCHHHHHHHHCCCCCCCCCCCC
LNDRHVRLNASWRNLASDRLAVWHHLVRRQQTAWPDGDFQSAFAQELDAAYRGRMAAGRL
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHCEE
YANDLFVTLVLHPGVDPAERTQAWVQRLIGRGVPAVDDADVSRLEEIAEDLAQHLRRYEP
ECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TRLGLRHLDGLVFSEPLEMLRLILTGRPEAVPLTRGHLGAALYASRLIFGREAIEIRDAA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
ETRYAGMLGLKEYPAATRPGHWDTLLSAPFPFVLSQSFAFLSKPAAQAVMSRKQNQMVST
HHHHHHHCCHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
RDRAASQIAGLDEALDDLTSNRFALGEHQASLLVLGDTARALADHMSRASALLAESGLVV
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEE
VREDLGLEAGFWAQFPGNFRYRLRPAAITSRNFAGLAPFHTHPAGRGHGNHWGEAVALLR
EECCCCCCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TTAGSPYHFNFHAGDLGHTFICGPSGSGKTVVQNFMLGQLEKLGCQQVFIDKDRGAEIFI
HCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEE
RACGGTYLTLRPGEPTGFAPLKAAADTPPDRAFLRALIRSLVAGADGALGVEDENAIERA
EECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VAAVLKLPADERSFAALRSLLGQREAGGIGARLDRWRQGRALGWALDGETDALDLSPRLA
HHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCEECCCCCC
GFDMTHLLDSAEVRTPLMLYLFHRLQKLVDGRRLVIDIDEFWKALGDEAFRGLAEDGLKT
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
FRKQNAFMVFGTQSPADVLRSPIAHTIVEQCATKIFLPNPHASERDYVDGFGLTCREFQL
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHH
IRDELSAEQRRFLVKQGLDSVVVELDLGGLEDALAVLSGRAETVDLLDRIRGIHGDDPTA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
WLAPFQQARREAR
HHHHHHHHHHHCC
>Mature Secondary Structure
MRPRRLPVASLSARRREPEPARFLPYALHVSDSVLALDSGALMTCFRLTGASFETADIRD
CCCCCCCHHHHHHHCCCCCCHHHCCEEEECCCCEEEECCCHHHHHHHHCCCCCCCCCCCC
LNDRHVRLNASWRNLASDRLAVWHHLVRRQQTAWPDGDFQSAFAQELDAAYRGRMAAGRL
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHCEE
YANDLFVTLVLHPGVDPAERTQAWVQRLIGRGVPAVDDADVSRLEEIAEDLAQHLRRYEP
ECCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
TRLGLRHLDGLVFSEPLEMLRLILTGRPEAVPLTRGHLGAALYASRLIFGREAIEIRDAA
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
ETRYAGMLGLKEYPAATRPGHWDTLLSAPFPFVLSQSFAFLSKPAAQAVMSRKQNQMVST
HHHHHHHCCHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
RDRAASQIAGLDEALDDLTSNRFALGEHQASLLVLGDTARALADHMSRASALLAESGLVV
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEE
VREDLGLEAGFWAQFPGNFRYRLRPAAITSRNFAGLAPFHTHPAGRGHGNHWGEAVALLR
EECCCCCCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TTAGSPYHFNFHAGDLGHTFICGPSGSGKTVVQNFMLGQLEKLGCQQVFIDKDRGAEIFI
HCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEE
RACGGTYLTLRPGEPTGFAPLKAAADTPPDRAFLRALIRSLVAGADGALGVEDENAIERA
EECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
VAAVLKLPADERSFAALRSLLGQREAGGIGARLDRWRQGRALGWALDGETDALDLSPRLA
HHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCEECCCCCC
GFDMTHLLDSAEVRTPLMLYLFHRLQKLVDGRRLVIDIDEFWKALGDEAFRGLAEDGLKT
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
FRKQNAFMVFGTQSPADVLRSPIAHTIVEQCATKIFLPNPHASERDYVDGFGLTCREFQL
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHH
IRDELSAEQRRFLVKQGLDSVVVELDLGGLEDALAVLSGRAETVDLLDRIRGIHGDDPTA
HHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
WLAPFQQARREAR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]