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The map label for this gene is ddl [H]

Identifier: 197105779

GI number: 197105779

Start: 2628053

End: 2629030

Strand: Reverse

Name: ddl [H]

Synonym: PHZ_c2317

Alternate gene names: 197105779

Gene position: 2629030-2628053 (Counterclockwise)

Preceding gene: 197105780

Following gene: 197105778

Centisome position: 65.79

GC content: 69.33

Gene sequence:

>978_bases
ATGGCAGCGCCGCTCTCCGGTCACCACGTCGCCGTCCTGATGGGCGGCCTGTCCTCCGAGCGGGAGGTGAGCCTCGTCTC
CGGCCGTGAGTGCGCCGACGCGCTCGAGCGGCTGGGCGCGAAGGTCAGCCGCATCGACGCCGGCCGCGACCTGGCCCAGG
TGCTCTCGGCCCTGAAGCCCGACGTCTGCTTCAACGCCCTGCACGGGGCCTGGGGCGAGGACGGCTGCGTCCAGGGCGTG
CTCGAGACGCTGGCCATCCCCTACACCCACTCGGGCGTGCTGGCCTCGGCCCTGGCCATGGACAAGGCCAAGGCCAAGGC
CGTGCTGGCCGCCGCGGGCGTGCCGGTGCCCGGCGGCGGGCTCTACAACCGCTACGACGTCGCCCGCGAGCACGTCATGG
CGCCGCCCTATGTGGTCAAGGCCAACGCCGAGGGCTCGTCCGTCGGCGTGTTCATCGTCCGCGACCGGGCGAACCGGCCG
CCGCAGGAGATCCTGGAACCCTCCTGGACCTACGGGGAGCAGGTGATGGTCGAGCCCTTCATCCGCGGCATGGAGCTGGC
CGTCGGCGTGATGGACGGCAAGGCCATGGAGGTCACCGAGATCCTGACCGAGCGTGAGTTCTACGACTACGAGGCCAAGT
ACGCAGAGGGCGGCTCGAAGCACATCGTCCCGGCCAGGATGCCGGCGCACGCGCTTGAAAAGGCGAAGGAATACTCCGAG
CGGGCGCACGCCGCGCTTGGTTGTCGCGGAGTTACCCGGTCGGACCTGCGTTATGACGACATTAACGACATTCTGGTCCT
CTTGGAGGTCAATACCCAGCCCGGCATGACGCCCACCTCTCTCGTTCCCGAGCAGGCGGCGGCTCAGGGCGTGGATTTCG
ACCGGCTCGTGTTGTGGATCACGGAGGACGCCTATGCCCGCGGCGGAGCGGGGAGGATCGCGAGTTCAGGCGAAGCCTCG
GGCCAAAGCGCCCCCTAG

Upstream 100 bases:

>100_bases
GGCTGCTGGGCCGGCGCGAGCGCGGCGGCGAGGCCCCTTGACCGCAGGGCCCCTGTAGGCGACGGCGGACGCAACCCCTT
CAGCTCTCGCGAGTCGCCCC

Downstream 100 bases:

>100_bases
TCCGGCCAGGCGCCCCGCGAAGGGCGGGGCCGGTTCGCCCGCGCGCGCGGGTCCGTCTCCCAAGCTGATCCTGCTGGCCG
CGGCCGGGGTGCTCGTCGCC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAAPLSGHHVAVLMGGLSSEREVSLVSGRECADALERLGAKVSRIDAGRDLAQVLSALKPDVCFNALHGAWGEDGCVQGV
LETLAIPYTHSGVLASALAMDKAKAKAVLAAAGVPVPGGGLYNRYDVAREHVMAPPYVVKANAEGSSVGVFIVRDRANRP
PQEILEPSWTYGEQVMVEPFIRGMELAVGVMDGKAMEVTEILTEREFYDYEAKYAEGGSKHIVPARMPAHALEKAKEYSE
RAHAALGCRGVTRSDLRYDDINDILVLLEVNTQPGMTPTSLVPEQAAAQGVDFDRLVLWITEDAYARGGAGRIASSGEAS
GQSAP

Sequences:

>Translated_325_residues
MAAPLSGHHVAVLMGGLSSEREVSLVSGRECADALERLGAKVSRIDAGRDLAQVLSALKPDVCFNALHGAWGEDGCVQGV
LETLAIPYTHSGVLASALAMDKAKAKAVLAAAGVPVPGGGLYNRYDVAREHVMAPPYVVKANAEGSSVGVFIVRDRANRP
PQEILEPSWTYGEQVMVEPFIRGMELAVGVMDGKAMEVTEILTEREFYDYEAKYAEGGSKHIVPARMPAHALEKAKEYSE
RAHAALGCRGVTRSDLRYDDINDILVLLEVNTQPGMTPTSLVPEQAAAQGVDFDRLVLWITEDAYARGGAGRIASSGEAS
GQSAP
>Mature_324_residues
AAPLSGHHVAVLMGGLSSEREVSLVSGRECADALERLGAKVSRIDAGRDLAQVLSALKPDVCFNALHGAWGEDGCVQGVL
ETLAIPYTHSGVLASALAMDKAKAKAVLAAAGVPVPGGGLYNRYDVAREHVMAPPYVVKANAEGSSVGVFIVRDRANRPP
QEILEPSWTYGEQVMVEPFIRGMELAVGVMDGKAMEVTEILTEREFYDYEAKYAEGGSKHIVPARMPAHALEKAKEYSER
AHAALGCRGVTRSDLRYDDINDILVLLEVNTQPGMTPTSLVPEQAAAQGVDFDRLVLWITEDAYARGGAGRIASSGEASG
QSAP

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=36.6013071895425, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786579, Length=356, Percent_Identity=30.0561797752809, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34571; Mature: 34439

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPLSGHHVAVLMGGLSSEREVSLVSGRECADALERLGAKVSRIDAGRDLAQVLSALKP
CCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCH
DVCFNALHGAWGEDGCVQGVLETLAIPYTHSGVLASALAMDKAKAKAVLAAAGVPVPGGG
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LYNRYDVAREHVMAPPYVVKANAEGSSVGVFIVRDRANRPPQEILEPSWTYGEQVMVEPF
CCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHH
IRGMELAVGVMDGKAMEVTEILTEREFYDYEAKYAEGGSKHIVPARMPAHALEKAKEYSE
HCCHHHEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RAHAALGCRGVTRSDLRYDDINDILVLLEVNTQPGMTPTSLVPEQAAAQGVDFDRLVLWI
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCCHHHHHCCCCCCCEEEEE
TEDAYARGGAGRIASSGEASGQSAP
ECHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAPLSGHHVAVLMGGLSSEREVSLVSGRECADALERLGAKVSRIDAGRDLAQVLSALKP
CCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCH
DVCFNALHGAWGEDGCVQGVLETLAIPYTHSGVLASALAMDKAKAKAVLAAAGVPVPGGG
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LYNRYDVAREHVMAPPYVVKANAEGSSVGVFIVRDRANRPPQEILEPSWTYGEQVMVEPF
CCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCHHHHHHH
IRGMELAVGVMDGKAMEVTEILTEREFYDYEAKYAEGGSKHIVPARMPAHALEKAKEYSE
HCCHHHEEEECCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RAHAALGCRGVTRSDLRYDDINDILVLLEVNTQPGMTPTSLVPEQAAAQGVDFDRLVLWI
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCCHHHHHCCCCCCCEEEEE
TEDAYARGGAGRIASSGEASGQSAP
ECHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]