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The map label for this gene is ligA

Identifier: 197105771

GI number: 197105771

Start: 2616252

End: 2618330

Strand: Reverse

Name: ligA

Synonym: PHZ_c2309

Alternate gene names: 197105771

Gene position: 2618330-2616252 (Counterclockwise)

Preceding gene: 197105772

Following gene: 197105770

Centisome position: 65.52

GC content: 71.04

Gene sequence:

>2079_bases
ATGAAGTCTGTCGAATCCCTCACCGAGGCCGAGGCCGCCGAAGAGCTGACCCGGCTCGCCGACGAGATCGCCGCGCACGA
CATCCGCTATTACCGCGAGGATGCGCCGACGATCTCGGACGCGGCGTACGACGCCCTGCGACGGCGGAACGACGAACTCG
AGGCGGCCTTCCCGCACCTGATCCGCGAGAACTCGCCGTCGCTGCGCATCGGCGCGGCCAGGGCCGAGCAGTTCGCGCCC
GTCGAGCATGGCGTGCCGATGCTCTCGCTGGACAACGCGTTCTCGGACGCGGACGCGCTGGAGTTCGACGCGCGGGTGAA
GCGCTTCCTGCGGCTGGACGAGGAGGTCGCCTACACGGCCGAGCCGAAGATCGACGGCCTGTCCTGCTCGATCCGCTACG
AGAACGGCCGCCTGGTGCGCGCCGCGACGCGAGGCGACGGCCGGGTGGGCGAGGACGTCACCGCCAACGTCCGCACCATC
GGGGAGATCCCGCAGAAGCTGCACGGCCAGGCCTGGCCCGAGGTCATCGAGATCCGCGGCGAAGTCTACCTCGGCCACGC
CGAGTTCGCCGCCCTGAACGAGGCCGCCGCCTCGGCCGGCCAGAAGACCTACGCCAACCCGCGCAACGCGGCGGCGGGCT
CGCTGCGCCAGATCGACCCGAAGATCACCGCCCAGCGCCCCCTGCGCTTCTTCGCCTACGCCTGGGGCTTCGTCAGCGCC
CCGTTCGCCCAGACCCAGTGGGAGGCGCTGCAGGCGCTGAAGGCCTGGGGCTTCCGCACCACGCCGCAGTCCCGCTGCGT
GAAAGGCGCCGAGGGCCTCATGGAGGCCTACCGCGAGATGGAGGCCGAGCGGCCGCACCTGGGCTTCGACATCGACGGGG
TGGTCTACAAGGTGGACCGCCTCGAGTGGCAGCAGCGCCTGGGCTTCGTCTCGCGCGCGCCGCGCTGGGGGATCGCCCGC
AAGTTCCCGGCCGAACAGGCGCGCACCGTGCTGGAGGCCATCGACATCCAGGTGGGCCGCACCGGCGCCCTGACTCCCGT
CGCGCGCCTGACGCCGGTGACCGTCGGCGGGGTGGTGGTCACCAACGCCACCTTGCACAACGCCGACGAGATCGCGCGCC
TCGACGTGCGCGTCGGCGACACGGTCGTGCTGCAGCGGGCCGGCGACGTCATCCCGCAGATCCTGCGCGTGATCCCCGAG
GAGCGGCCGAAGGACTCCGTCCCCTACGACTTCCCGCACGTCTGCCCGTGCCCGCTCCGGACCGAGGTCGTCCGCGAGAC
CACCGCCTCGGGCGCCGAGACCGTGGTGCGCCGCTGCTCGGGCGAGTTCGCCTGCCCGTTCCAGCGCATCAATCACCTGC
TGCACTTCGTTTCCCGCCGGGCGTTCGACATTGAGGGCCTGGGCGAGAAGCAGCTCACCGCCTTCTTCGAGCGCGGCTGG
ATCAAGGCGCCGGCCGACATCTTCCGCCTGCACGAGAAGCGCGACGAGCTGCTGGCCATGGAGCGGATGGGCGAGACCAG
CGTGGGCAACCTGCTGGCGAACATCGAGGCCCGCCGCACCATCGGCCTGGACCGCTTCATCTACGGCCTGGGGATCCGCC
ACATCGGGGAGACCACCGCGACGGTGATGGCGCGCGGCTACGGCACGGCCGCCCACTTCCTGGAAGCGATGGACAAGGTG
GCGGCCCGCGATCCCGAGGCGGTCGAGGAGCTGGACGCCCTCGACCAGATCGGCGGGGCGGTGATCGAGGCCGCCGCGGC
CTTCTTCGGCGAGGACCACAACCGGGCCATGGTCGAGGACCTGGCGGGCCAGCTCACCATCCTCGACGCCGAGAAGCCCA
AGACCGACACGGCGGTGGCCGGCAAGACCGTGGTCTTCACCGGCGCGCTGGAGCGGATGACCCGCGACGAGGCCAAGGCC
CAGGCCGAGCGGCTGGGGGCCAAGGTGTCGGGCTCGGTGTCGAAGAAGACCGACATCGTGGTGGCCGGTCCCGGCGCAGG
CTCGAAGCTGAAGACGGCCGCCGAGCTCGGCATCCAGGTGCTCACCGAAGACGAATGGTTGGAGATGGTCGGCAGCTAG

Upstream 100 bases:

>100_bases
ACCGACGCCGCCCGCGCGGCCGCCCGCGCGCTTATCGGCGCCTGAATCCGCGCGCGCTCCCGCTTCGCGGCGGAGGGAGG
CCGCGCTAGACCTTCGCCCG

Downstream 100 bases:

>100_bases
GAGCCCGAACTCTCCCGTGATCCCCTCGTCCCGCTTGAAGGTCCTCGTGGACGCGCCGCCGCGTCCCGGGGCCCGCTACG
TGCTCTACTGGATGCAGCAG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MKSVESLTEAEAAEELTRLADEIAAHDIRYYREDAPTISDAAYDALRRRNDELEAAFPHLIRENSPSLRIGAARAEQFAP
VEHGVPMLSLDNAFSDADALEFDARVKRFLRLDEEVAYTAEPKIDGLSCSIRYENGRLVRAATRGDGRVGEDVTANVRTI
GEIPQKLHGQAWPEVIEIRGEVYLGHAEFAALNEAAASAGQKTYANPRNAAAGSLRQIDPKITAQRPLRFFAYAWGFVSA
PFAQTQWEALQALKAWGFRTTPQSRCVKGAEGLMEAYREMEAERPHLGFDIDGVVYKVDRLEWQQRLGFVSRAPRWGIAR
KFPAEQARTVLEAIDIQVGRTGALTPVARLTPVTVGGVVVTNATLHNADEIARLDVRVGDTVVLQRAGDVIPQILRVIPE
ERPKDSVPYDFPHVCPCPLRTEVVRETTASGAETVVRRCSGEFACPFQRINHLLHFVSRRAFDIEGLGEKQLTAFFERGW
IKAPADIFRLHEKRDELLAMERMGETSVGNLLANIEARRTIGLDRFIYGLGIRHIGETTATVMARGYGTAAHFLEAMDKV
AARDPEAVEELDALDQIGGAVIEAAAAFFGEDHNRAMVEDLAGQLTILDAEKPKTDTAVAGKTVVFTGALERMTRDEAKA
QAERLGAKVSGSVSKKTDIVVAGPGAGSKLKTAAELGIQVLTEDEWLEMVGS

Sequences:

>Translated_692_residues
MKSVESLTEAEAAEELTRLADEIAAHDIRYYREDAPTISDAAYDALRRRNDELEAAFPHLIRENSPSLRIGAARAEQFAP
VEHGVPMLSLDNAFSDADALEFDARVKRFLRLDEEVAYTAEPKIDGLSCSIRYENGRLVRAATRGDGRVGEDVTANVRTI
GEIPQKLHGQAWPEVIEIRGEVYLGHAEFAALNEAAASAGQKTYANPRNAAAGSLRQIDPKITAQRPLRFFAYAWGFVSA
PFAQTQWEALQALKAWGFRTTPQSRCVKGAEGLMEAYREMEAERPHLGFDIDGVVYKVDRLEWQQRLGFVSRAPRWGIAR
KFPAEQARTVLEAIDIQVGRTGALTPVARLTPVTVGGVVVTNATLHNADEIARLDVRVGDTVVLQRAGDVIPQILRVIPE
ERPKDSVPYDFPHVCPCPLRTEVVRETTASGAETVVRRCSGEFACPFQRINHLLHFVSRRAFDIEGLGEKQLTAFFERGW
IKAPADIFRLHEKRDELLAMERMGETSVGNLLANIEARRTIGLDRFIYGLGIRHIGETTATVMARGYGTAAHFLEAMDKV
AARDPEAVEELDALDQIGGAVIEAAAAFFGEDHNRAMVEDLAGQLTILDAEKPKTDTAVAGKTVVFTGALERMTRDEAKA
QAERLGAKVSGSVSKKTDIVVAGPGAGSKLKTAAELGIQVLTEDEWLEMVGS
>Mature_692_residues
MKSVESLTEAEAAEELTRLADEIAAHDIRYYREDAPTISDAAYDALRRRNDELEAAFPHLIRENSPSLRIGAARAEQFAP
VEHGVPMLSLDNAFSDADALEFDARVKRFLRLDEEVAYTAEPKIDGLSCSIRYENGRLVRAATRGDGRVGEDVTANVRTI
GEIPQKLHGQAWPEVIEIRGEVYLGHAEFAALNEAAASAGQKTYANPRNAAAGSLRQIDPKITAQRPLRFFAYAWGFVSA
PFAQTQWEALQALKAWGFRTTPQSRCVKGAEGLMEAYREMEAERPHLGFDIDGVVYKVDRLEWQQRLGFVSRAPRWGIAR
KFPAEQARTVLEAIDIQVGRTGALTPVARLTPVTVGGVVVTNATLHNADEIARLDVRVGDTVVLQRAGDVIPQILRVIPE
ERPKDSVPYDFPHVCPCPLRTEVVRETTASGAETVVRRCSGEFACPFQRINHLLHFVSRRAFDIEGLGEKQLTAFFERGW
IKAPADIFRLHEKRDELLAMERMGETSVGNLLANIEARRTIGLDRFIYGLGIRHIGETTATVMARGYGTAAHFLEAMDKV
AARDPEAVEELDALDQIGGAVIEAAAAFFGEDHNRAMVEDLAGQLTILDAEKPKTDTAVAGKTVVFTGALERMTRDEAKA
QAERLGAKVSGSVSKKTDIVVAGPGAGSKLKTAAELGIQVLTEDEWLEMVGS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=687, Percent_Identity=47.3071324599709, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI87082305, Length=473, Percent_Identity=26.6384778012685, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PHEZH (B4RFE9)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002131148.1
- ProteinModelPortal:   B4RFE9
- GeneID:   6791830
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c2309
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75990; Mature: 75990

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 123-123 BINDING 121-121 BINDING 144-144 BINDING 181-181 BINDING 297-297 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVESLTEAEAAEELTRLADEIAAHDIRYYREDAPTISDAAYDALRRRNDELEAAFPHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHH
IRENSPSLRIGAARAEQFAPVEHGVPMLSLDNAFSDADALEFDARVKRFLRLDEEVAYTA
HHCCCCCEEEEHHHHHHCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHEEEC
EPKIDGLSCSIRYENGRLVRAATRGDGRVGEDVTANVRTIGEIPQKLHGQAWPEVIEIRG
CCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC
EVYLGHAEFAALNEAAASAGQKTYANPRNAAAGSLRQIDPKITAQRPLRFFAYAWGFVSA
CEEECCHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHCCCCCCHHCHHHHHHHHHHHHCC
PFAQTQWEALQALKAWGFRTTPQSRCVKGAEGLMEAYREMEAERPHLGFDIDGVVYKVDR
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHH
LEWQQRLGFVSRAPRWGIARKFPAEQARTVLEAIDIQVGRTGALTPVARLTPVTVGGVVV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEECCEEE
TNATLHNADEIARLDVRVGDTVVLQRAGDVIPQILRVIPEERPKDSVPYDFPHVCPCPLR
ECCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TEVVRETTASGAETVVRRCSGEFACPFQRINHLLHFVSRRAFDIEGLGEKQLTAFFERGW
HHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
IKAPADIFRLHEKRDELLAMERMGETSVGNLLANIEARRTIGLDRFIYGLGIRHIGETTA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TVMARGYGTAAHFLEAMDKVAARDPEAVEELDALDQIGGAVIEAAAAFFGEDHNRAMVED
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LAGQLTILDAEKPKTDTAVAGKTVVFTGALERMTRDEAKAQAERLGAKVSGSVSKKTDIV
HCCCEEEEECCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEE
VAGPGAGSKLKTAAELGIQVLTEDEWLEMVGS
EECCCCCCHHHHHHHCCEEEECCHHHHHHHCC
>Mature Secondary Structure
MKSVESLTEAEAAEELTRLADEIAAHDIRYYREDAPTISDAAYDALRRRNDELEAAFPHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHH
IRENSPSLRIGAARAEQFAPVEHGVPMLSLDNAFSDADALEFDARVKRFLRLDEEVAYTA
HHCCCCCEEEEHHHHHHCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHEEEC
EPKIDGLSCSIRYENGRLVRAATRGDGRVGEDVTANVRTIGEIPQKLHGQAWPEVIEIRG
CCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCC
EVYLGHAEFAALNEAAASAGQKTYANPRNAAAGSLRQIDPKITAQRPLRFFAYAWGFVSA
CEEECCHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHCCCCCCHHCHHHHHHHHHHHHCC
PFAQTQWEALQALKAWGFRTTPQSRCVKGAEGLMEAYREMEAERPHLGFDIDGVVYKVDR
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHH
LEWQQRLGFVSRAPRWGIARKFPAEQARTVLEAIDIQVGRTGALTPVARLTPVTVGGVVV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEECCEEE
TNATLHNADEIARLDVRVGDTVVLQRAGDVIPQILRVIPEERPKDSVPYDFPHVCPCPLR
ECCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
TEVVRETTASGAETVVRRCSGEFACPFQRINHLLHFVSRRAFDIEGLGEKQLTAFFERGW
HHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
IKAPADIFRLHEKRDELLAMERMGETSVGNLLANIEARRTIGLDRFIYGLGIRHIGETTA
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
TVMARGYGTAAHFLEAMDKVAARDPEAVEELDALDQIGGAVIEAAAAFFGEDHNRAMVED
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LAGQLTILDAEKPKTDTAVAGKTVVFTGALERMTRDEAKAQAERLGAKVSGSVSKKTDIV
HCCCEEEEECCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEE
VAGPGAGSKLKTAAELGIQVLTEDEWLEMVGS
EECCCCCCHHHHHHHCCEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA