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The map label for this gene is leuA [H]

Identifier: 197105751

GI number: 197105751

Start: 2593115

End: 2594698

Strand: Reverse

Name: leuA [H]

Synonym: PHZ_c2289

Alternate gene names: 197105751

Gene position: 2594698-2593115 (Counterclockwise)

Preceding gene: 197105752

Following gene: 197105750

Centisome position: 64.93

GC content: 67.99

Gene sequence:

>1584_bases
ATGACCGCCGACGTATCCAAGACCGCCGCTCAGGCCGAGCGCGACCGCGTCATCATCTTCGACACCACCATGCGCGACGG
CGAGCAGTCGCCCGGAGCCTCCATGTCGCTGGAGGAGAAGCTGGAGCTCGCCAAGATCCTGGAGGATATGCGCGTCGACG
TGATCGAGGCCGGCTTCCCGATCGCCTCGAACGGCGACTTCGAGGCAGTGCGCCAGATCTCCGAGGTCGTCACCGAGAGC
GTCGTCTGCGGCCTGGCCCGCGCCGGCGCCCCCGACATCGATCGCTGCGCCGAGGCGATCAAGCGCGCCAAGCGCGGCCG
CATCCACACGTTCATCTCCACCAGCCCGGTCCACATGAAGTGGAAGCTGCAGATGGAGCCCGAGCAGGTGCTGGAGATGG
TGACCCGCTCGGTCGGCCACGCCCGCAACCTGTGCGGCGACGTGGAATGGTCGGCCGAGGACGCCACCCGTACCGAGCGC
GACTTCCTGCTGCGCTGCGTCGAGGCGGCGATCAAGGCGGGCGCCACGACGATCAACCTGCCCGACACGGTGGGCTACTC
GTACCCCAGCGAGTACGCCGAGATGTTCCGCGACGTGATCGAGAACGTGCCCGGCGCGGACAAGGTCGTCTTCTCCACCC
ACTGCCACAACGACCTGGGGCTGGCGGTCGCCAACTCCATCGCCGGCGTCCAGGGCGGCGCACGCCAGATCGAGTGCGCC
GTCAACGGCATCGGCGAGCGGGCCGGCAACGCGGCGCTGGAAGAGATCGTGATGGCGCTGAAGGTGCGGGGCGACAAGCT
GCCGTTCCACACCGCCGTCGAGACCCAGCACATCACCCGCGCCAGCCGCTACGTCTCGGCCATCACCGGCTTCCCCGTGC
AGTTCAACAAGGCGATCGTCGGCAAGAACGCCTTCGCCCACGAGAGCGGCATCCACCAGGACGGGATGCTGAAGAACGCC
GAGACCTACGAGATCATGAAGCCCGAGGACGTGGGCCAGGTGGAGTCCAACCTGGTCATGGGCAAGCACTCGGGCCGGCA
CGCCTTCCGCGAGAAGCTGAAGGACCTGGGTTACGAGCTGGGCCAGAACGCCCTGAACGAGGCGTTCCAGCGCTTCAAGG
ACCTGGCCGACAAGAAGAAGCACGTCTTCGACGACGACATCATCGCCCTGGTGGACGACGCCCTGGCGAGCTCGGCCGAC
AAGGTCCAGGTCAAGCACCTGCGGGTGATCGCCGGCACCGAAGGGCCGCAGGAAGCCTTCCTGACGGTCACCATCGACGG
CGAGGAGAAGAGCGCCAACGCCACCGGCGACGGCCCGGTCGACGCGGTGTTCAACGCCATCCATGCGGTGGTGCCGCACG
ACGCCATCCTGCGCCTGTTCCAGGTGCACGCCGTGACGGAAGGGACCGACGCCCAGGCGCAGGTCTCGGTGCGGCTCGAG
GACGACGGACGGATCGCCACCGGCCAGGCGGCCGACACCGACACCCTGACCGCCTCGGCCAAGGCCTACGTCAACGCGCT
CAACAACCTCATCGCGCGCAAGGAGAAGAGCGCGCCCGAGGCGCGGGTCGCCAGCGGGTTCTAG

Upstream 100 bases:

>100_bases
CCTGGGCGCCTCTCTAGGCACGCGCATCTTCGCGGCCGGGCGCTCAGGGGCTTTCCGATCCCCCGCCCGAAACCGAAATC
CTCGCGAAGAGCCTCCCGAC

Downstream 100 bases:

>100_bases
GCGGCTTCCTCTCCCCCGCGGGGAGAGGCGCTCCGATGCTCTGGATCATCCTCACCGTGGCGGCCGCGCCGCTGCAGGTG
GCGCGCAACGCGTTCCAGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTADVSKTAAQAERDRVIIFDTTMRDGEQSPGASMSLEEKLELAKILEDMRVDVIEAGFPIASNGDFEAVRQISEVVTES
VVCGLARAGAPDIDRCAEAIKRAKRGRIHTFISTSPVHMKWKLQMEPEQVLEMVTRSVGHARNLCGDVEWSAEDATRTER
DFLLRCVEAAIKAGATTINLPDTVGYSYPSEYAEMFRDVIENVPGADKVVFSTHCHNDLGLAVANSIAGVQGGARQIECA
VNGIGERAGNAALEEIVMALKVRGDKLPFHTAVETQHITRASRYVSAITGFPVQFNKAIVGKNAFAHESGIHQDGMLKNA
ETYEIMKPEDVGQVESNLVMGKHSGRHAFREKLKDLGYELGQNALNEAFQRFKDLADKKKHVFDDDIIALVDDALASSAD
KVQVKHLRVIAGTEGPQEAFLTVTIDGEEKSANATGDGPVDAVFNAIHAVVPHDAILRLFQVHAVTEGTDAQAQVSVRLE
DDGRIATGQAADTDTLTASAKAYVNALNNLIARKEKSAPEARVASGF

Sequences:

>Translated_527_residues
MTADVSKTAAQAERDRVIIFDTTMRDGEQSPGASMSLEEKLELAKILEDMRVDVIEAGFPIASNGDFEAVRQISEVVTES
VVCGLARAGAPDIDRCAEAIKRAKRGRIHTFISTSPVHMKWKLQMEPEQVLEMVTRSVGHARNLCGDVEWSAEDATRTER
DFLLRCVEAAIKAGATTINLPDTVGYSYPSEYAEMFRDVIENVPGADKVVFSTHCHNDLGLAVANSIAGVQGGARQIECA
VNGIGERAGNAALEEIVMALKVRGDKLPFHTAVETQHITRASRYVSAITGFPVQFNKAIVGKNAFAHESGIHQDGMLKNA
ETYEIMKPEDVGQVESNLVMGKHSGRHAFREKLKDLGYELGQNALNEAFQRFKDLADKKKHVFDDDIIALVDDALASSAD
KVQVKHLRVIAGTEGPQEAFLTVTIDGEEKSANATGDGPVDAVFNAIHAVVPHDAILRLFQVHAVTEGTDAQAQVSVRLE
DDGRIATGQAADTDTLTASAKAYVNALNNLIARKEKSAPEARVASGF
>Mature_526_residues
TADVSKTAAQAERDRVIIFDTTMRDGEQSPGASMSLEEKLELAKILEDMRVDVIEAGFPIASNGDFEAVRQISEVVTESV
VCGLARAGAPDIDRCAEAIKRAKRGRIHTFISTSPVHMKWKLQMEPEQVLEMVTRSVGHARNLCGDVEWSAEDATRTERD
FLLRCVEAAIKAGATTINLPDTVGYSYPSEYAEMFRDVIENVPGADKVVFSTHCHNDLGLAVANSIAGVQGGARQIECAV
NGIGERAGNAALEEIVMALKVRGDKLPFHTAVETQHITRASRYVSAITGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAE
TYEIMKPEDVGQVESNLVMGKHSGRHAFREKLKDLGYELGQNALNEAFQRFKDLADKKKHVFDDDIIALVDDALASSADK
VQVKHLRVIAGTEGPQEAFLTVTIDGEEKSANATGDGPVDAVFNAIHAVVPHDAILRLFQVHAVTEGTDAQAQVSVRLED
DGRIATGQAADTDTLTASAKAYVNALNNLIARKEKSAPEARVASGF

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=47.7822580645161, Blast_Score=435, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320019, Length=372, Percent_Identity=27.1505376344086, Blast_Score=136, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=372, Percent_Identity=27.1505376344086, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=546, Percent_Identity=26.3736263736264, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=552, Percent_Identity=25.9057971014493, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57046; Mature: 56915

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADVSKTAAQAERDRVIIFDTTMRDGEQSPGASMSLEEKLELAKILEDMRVDVIEAGFP
CCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
IASNGDFEAVRQISEVVTESVVCGLARAGAPDIDRCAEAIKRAKRGRIHTFISTSPVHMK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEE
WKLQMEPEQVLEMVTRSVGHARNLCGDVEWSAEDATRTERDFLLRCVEAAIKAGATTINL
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECC
PDTVGYSYPSEYAEMFRDVIENVPGADKVVFSTHCHNDLGLAVANSIAGVQGGARQIECA
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEHHH
VNGIGERAGNAALEEIVMALKVRGDKLPFHTAVETQHITRASRYVSAITGFPVQFNKAIV
HHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHH
GKNAFAHESGIHQDGMLKNAETYEIMKPEDVGQVESNLVMGKHSGRHAFREKLKDLGYEL
CCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHCEEECCCCCHHHHHHHHHHHHHHH
GQNALNEAFQRFKDLADKKKHVFDDDIIALVDDALASSADKVQVKHLRVIAGTEGPQEAF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEE
LTVTIDGEEKSANATGDGPVDAVFNAIHAVVPHDAILRLFQVHAVTEGTDAQAQVSVRLE
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEEC
DDGRIATGQAADTDTLTASAKAYVNALNNLIARKEKSAPEARVASGF
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
TADVSKTAAQAERDRVIIFDTTMRDGEQSPGASMSLEEKLELAKILEDMRVDVIEAGFP
CCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
IASNGDFEAVRQISEVVTESVVCGLARAGAPDIDRCAEAIKRAKRGRIHTFISTSPVHMK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEE
WKLQMEPEQVLEMVTRSVGHARNLCGDVEWSAEDATRTERDFLLRCVEAAIKAGATTINL
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECC
PDTVGYSYPSEYAEMFRDVIENVPGADKVVFSTHCHNDLGLAVANSIAGVQGGARQIECA
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEHHH
VNGIGERAGNAALEEIVMALKVRGDKLPFHTAVETQHITRASRYVSAITGFPVQFNKAIV
HHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHH
GKNAFAHESGIHQDGMLKNAETYEIMKPEDVGQVESNLVMGKHSGRHAFREKLKDLGYEL
CCCCHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHCEEECCCCCHHHHHHHHHHHHHHH
GQNALNEAFQRFKDLADKKKHVFDDDIIALVDDALASSADKVQVKHLRVIAGTEGPQEAF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEE
LTVTIDGEEKSANATGDGPVDAVFNAIHAVVPHDAILRLFQVHAVTEGTDAQAQVSVRLE
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEEEC
DDGRIATGQAADTDTLTASAKAYVNALNNLIARKEKSAPEARVASGF
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA