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The map label for this gene is yogA [H]

Identifier: 197105732

GI number: 197105732

Start: 2571998

End: 2573017

Strand: Reverse

Name: yogA [H]

Synonym: PHZ_c2270

Alternate gene names: 197105732

Gene position: 2573017-2571998 (Counterclockwise)

Preceding gene: 197105733

Following gene: 197105731

Centisome position: 64.39

GC content: 69.51

Gene sequence:

>1020_bases
ATGCGTGCGCTGAACGTCACCGCCCCCTGGGGGCCGGACGCCATCCAGGTGGTGGAGGTCCCAGAGCCGACTCCGGGGCC
GGGCCAGGTCCTGGTGCGGATGAAGGCCTGCTCGCTGAACTATCGCGACTTCCTGATGATCCACGGGATGTACGGGCGGG
CGGCGGCCAGCGCGACCGACATCATCACGCCCTTCTCGGACGGCTGCGGCTACGTCGAGGCCGTGGGGCCCGGCGTCACC
CGCTTCAAGGCCGGCGACCGGGTCTCCACTCTGTTCTTCCAGAACTGGGTGTCGGGACCGCCGACGCTGGAGAAGCTGTC
GAGCGCCCTGGGCTTCCCGATCCCGGGGGCGGGCGCCGAGCTGCAGGTGTTCAGCCAGGAAGGGCTCTCGAAGGTTCCGG
ACTTCCTCACTGACCACGAGGTCGCCACTCTGCCTTGCGCGGCGCTGACCGCCTGGCGCGGGCTCTTTCACGACGCCCGC
CTGGAGCCGGGCGACACCGTGGTGCTGCAGGGGACGGGCGGGGTCTCGATCTTCGGCCTGCAGTTCGCCAAGGCCGCGGG
CTACCGCACGCTGATCACCTCGTCGTCGGACGCCAAGCTCGAGCGCGCCAAGGCGCTCGGGGCCGACCACCTCGTGAACT
ACAAGACAACCCCGGCCTGGTCGAAACCGGTGCGCGAGGCCACCGGCGGCCGCGGCGCGGACTTCATCATGGAGGTCGGC
GGCGGGGGCACGATCCAGGAGAGCATGAAGGCCATCAAGATCGGCGGCCACATCGCGGTGATCGGCATCGTGGCCGGCGC
GGGCGAGCCGTTCAATCCGGCCGCCCTGATCGGCAACTCCGCCAAGCTGCAGGGCCTGTCGGTCGGCTCACGCGACATGT
TCGAGTCCATGTGCCGGGCGATCGAGCTGCACGGCGTCCGCCCGGTGGTGGACAAGGTGTATCCGTGGACCGAGGCAAAG
GCCGCCATCGCGGCCATGGGCGGCGGCGAGCATTTCGGAAAGATCGTGCTGGAGTTCTGA

Upstream 100 bases:

>100_bases
CGAGCGGCCCGTGCTGCCCTCCACGCGAGGCTGACGTGGCGACACGGTTGCGGCGCGCCAGCGTCGCTCCATGATGCCTC
CCCAAGTGGAGGAAGTCGTC

Downstream 100 bases:

>100_bases
CCGGCGAGCCAAGGGAGGACGCCATGAAGACCAAGGGGATCAACCACCTGGCGCTCGTGTGCCGGGATATGGCCGAGACG
GTGGCGTTCTACACCCAGGT

Product: NADPH:quinone reductase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRALNVTAPWGPDAIQVVEVPEPTPGPGQVLVRMKACSLNYRDFLMIHGMYGRAAASATDIITPFSDGCGYVEAVGPGVT
RFKAGDRVSTLFFQNWVSGPPTLEKLSSALGFPIPGAGAELQVFSQEGLSKVPDFLTDHEVATLPCAALTAWRGLFHDAR
LEPGDTVVLQGTGGVSIFGLQFAKAAGYRTLITSSSDAKLERAKALGADHLVNYKTTPAWSKPVREATGGRGADFIMEVG
GGGTIQESMKAIKIGGHIAVIGIVAGAGEPFNPAALIGNSAKLQGLSVGSRDMFESMCRAIELHGVRPVVDKVYPWTEAK
AAIAAMGGGEHFGKIVLEF

Sequences:

>Translated_339_residues
MRALNVTAPWGPDAIQVVEVPEPTPGPGQVLVRMKACSLNYRDFLMIHGMYGRAAASATDIITPFSDGCGYVEAVGPGVT
RFKAGDRVSTLFFQNWVSGPPTLEKLSSALGFPIPGAGAELQVFSQEGLSKVPDFLTDHEVATLPCAALTAWRGLFHDAR
LEPGDTVVLQGTGGVSIFGLQFAKAAGYRTLITSSSDAKLERAKALGADHLVNYKTTPAWSKPVREATGGRGADFIMEVG
GGGTIQESMKAIKIGGHIAVIGIVAGAGEPFNPAALIGNSAKLQGLSVGSRDMFESMCRAIELHGVRPVVDKVYPWTEAK
AAIAAMGGGEHFGKIVLEF
>Mature_339_residues
MRALNVTAPWGPDAIQVVEVPEPTPGPGQVLVRMKACSLNYRDFLMIHGMYGRAAASATDIITPFSDGCGYVEAVGPGVT
RFKAGDRVSTLFFQNWVSGPPTLEKLSSALGFPIPGAGAELQVFSQEGLSKVPDFLTDHEVATLPCAALTAWRGLFHDAR
LEPGDTVVLQGTGGVSIFGLQFAKAAGYRTLITSSSDAKLERAKALGADHLVNYKTTPAWSKPVREATGGRGADFIMEVG
GGGTIQESMKAIKIGGHIAVIGIVAGAGEPFNPAALIGNSAKLQGLSVGSRDMFESMCRAIELHGVRPVVDKVYPWTEAK
AAIAAMGGGEHFGKIVLEF

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=357, Percent_Identity=28.8515406162465, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI22538444, Length=357, Percent_Identity=28.8515406162465, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194239674, Length=346, Percent_Identity=26.0115606936416, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI13236495, Length=346, Percent_Identity=26.0115606936416, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI18379349, Length=338, Percent_Identity=25.1479289940828, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI194239676, Length=346, Percent_Identity=25.1445086705202, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI28557745, Length=279, Percent_Identity=28.3154121863799, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI47519420, Length=353, Percent_Identity=25.4957507082153, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI24308257, Length=366, Percent_Identity=22.9508196721311, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI87081918, Length=322, Percent_Identity=26.3975155279503, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082125, Length=346, Percent_Identity=27.1676300578035, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790045, Length=345, Percent_Identity=24.9275362318841, Blast_Score=61, Evalue=9e-11,
Organism=Escherichia coli, GI1790485, Length=258, Percent_Identity=24.4186046511628, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17507255, Length=321, Percent_Identity=27.1028037383178, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556000, Length=280, Percent_Identity=27.5, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=351, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=294, Percent_Identity=24.4897959183673, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323961, Length=294, Percent_Identity=24.4897959183673, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1

Molecular weight: Translated: 35612; Mature: 35612

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALNVTAPWGPDAIQVVEVPEPTPGPGQVLVRMKACSLNYRDFLMIHGMYGRAAASATD
CCEEEECCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHCCHHH
IITPFSDGCGYVEAVGPGVTRFKAGDRVSTLFFQNWVSGPPTLEKLSSALGFPIPGAGAE
EECCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCE
LQVFSQEGLSKVPDFLTDHEVATLPCAALTAWRGLFHDARLEPGDTVVLQGTGGVSIFGL
EEEECHHCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEE
QFAKAAGYRTLITSSSDAKLERAKALGADHLVNYKTTPAWSKPVREATGGRGADFIMEVG
HHHHHCCCEEEEECCCCHHHHHHHHCCHHHEECCCCCCCCCHHHHHHCCCCCCEEEEEEC
GGGTIQESMKAIKIGGHIAVIGIVAGAGEPFNPAALIGNSAKLQGLSVGSRDMFESMCRA
CCCCHHHHHHHEEECCEEEEEEEEECCCCCCCCHHEECCCCEEEEECCCCHHHHHHHHHH
IELHGVRPVVDKVYPWTEAKAAIAAMGGGEHFGKIVLEF
HHHCCCCHHHHHCCCCCHHHHHEEECCCCCCCCEEEEEC
>Mature Secondary Structure
MRALNVTAPWGPDAIQVVEVPEPTPGPGQVLVRMKACSLNYRDFLMIHGMYGRAAASATD
CCEEEECCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCHHCCHHH
IITPFSDGCGYVEAVGPGVTRFKAGDRVSTLFFQNWVSGPPTLEKLSSALGFPIPGAGAE
EECCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCE
LQVFSQEGLSKVPDFLTDHEVATLPCAALTAWRGLFHDARLEPGDTVVLQGTGGVSIFGL
EEEECHHCHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEE
QFAKAAGYRTLITSSSDAKLERAKALGADHLVNYKTTPAWSKPVREATGGRGADFIMEVG
HHHHHCCCEEEEECCCCHHHHHHHHCCHHHEECCCCCCCCCHHHHHHCCCCCCEEEEEEC
GGGTIQESMKAIKIGGHIAVIGIVAGAGEPFNPAALIGNSAKLQGLSVGSRDMFESMCRA
CCCCHHHHHHHEEECCEEEEEEEEECCCCCCCCHHEECCCCEEEEECCCCHHHHHHHHHH
IELHGVRPVVDKVYPWTEAKAAIAAMGGGEHFGKIVLEF
HHHCCCCHHHHHCCCCCHHHHHEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.005 {cyclohexanol}} 0.004 {

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]