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The map label for this gene is btuB [H]

Identifier: 197105575

GI number: 197105575

Start: 2374480

End: 2377290

Strand: Reverse

Name: btuB [H]

Synonym: PHZ_c2112

Alternate gene names: 197105575

Gene position: 2377290-2374480 (Counterclockwise)

Preceding gene: 197105576

Following gene: 197105573

Centisome position: 59.49

GC content: 69.65

Gene sequence:

>2811_bases
ATGCTTCGATCCGCATGGCGGGGGGCGCTGGCCGCCTCCACCGCGACCGCGGCGCTGCTGGCGGTCGCGCCCGCGGCACA
GGCCCAGGACGGCGAAGTCGCCGAGGTCGTGGTCACCGGCTCGCGCATCGCGCGCCAGGACCTGGTGAGCTCCAGCCCGG
TGGCCACCGTGAGCCAGGAGGAGATCGCGGTCGTCGGCGCCAACAACGTCGAGGAGTATCTCAACTCGCTGCCGCAGCTG
ATCGCCGGCGCGACCAAGTCGACCAACGTGGCCGGCTCGGCCGACGCGACCGCGACGCTGAACCTGCGCGGCCTGGGCGC
CAAGCGGACGCTGGTGCTGGTGAACGGCCGCCGCTTCGTGCCGACCACCCAGGAAGGCATCGTCGACGTCAACAACATCC
CGGCCTCGCTCGTCGAGCGCGTCGAGGTGGTCACCGGCGGCGCCTCGGCCGTGTACGGCTCGGACGCCATGGCCGGCGTC
GTGAACTTCATCCTGAAGGACCGCTTCCAGGGCCTCGAGATGGACGCCCAGTACGGCGTCTCTGACGAGGGCGACGCCGA
CGCCTGGAACGCCTCGGCCACCATGGGCGCGGCGGGTGAGAAGGCCTCGGCCTGGCTGCACGTCAGCCTGGAGCAGCGCA
AGAAGCTGCGCGGCACCGAGCGTCCGATCTCGGTCGTCAGCTACATCGACGCCAACGGCAACTTCGTGCCGTCCGGCTCG
GCCGGCCGCCTTGGCGGCACCCTGGTCGGCATTCCGACGCCGAACGGCTCGGGCGGCTTCGTCAACCGCGACTACGCGCT
GGACAACATGATCCCGCGTCCGTTCGTGAACTCCGACTTCAACTTCGACGCCCGCGGCGACTACGCGATCCAGATCCCGC
TCGACCGCGTCAACGTCTACGGCCGCGGCGAGTACGAGTTCACCCCGGACATCTCCGGCTTCGTCGAGGTGACCTACAAC
AACCTCGACTCGCGGGCGATCCTGTCGCCGACCGCGCCGAACATCCGCCAGACGACCAACGTCCCCGGCCTGCCGGCGAA
CGCCTCGTTCATCACGCCCGAGATCCGCGCGCTGCTGAACGCCCGGCCCGATCCGAACGCGCCGTTCTCGCTGCGCTTCC
TGGCGCCCGAGCCGTTCCCCAAGCGCGAGATCGCCTTCAACCGCGACATCTACCGCCTGCTGGGCGGCTTCGAGGGCCGC
CTGTCCGACAGCTGGCAGTGGGGCGTGAGCTACAGCTACAGCCACGTCGCCACGACCGAGGTGCAGAAGGGCGACATCTC
CCGCCGGACCTTCGTCGAGGCCTCGACGCCGAGCGCGGCCGATCCGACCAAGTGCGCCAACGGCAACCCGCGCTGCGTGC
TGGTCACCAACCTGACCAACTGGTCGCCCGAGCTGGTCGACTATCTGCTGGTCGACAACATCAGCTCGACCGAGATCACC
GACCAGGTGGCCTCGGCCTACGCCACCGGCGACCTGTTCACCCTGCCCGCGGGCGAGGTGAAGGCGGCCATCGGCGCCGA
GTGGCGGAAGATCTCCAGCGTCGACAACCCGGCGCCGGTGCTGCTCGACTTCGCCTCGGCCGGCTTCGGCGAGCGCAACC
GCACCGCCGGCTCGTACAGCGTCAAGGAGCTGTTCGGCGAGGCGATCGTGCCGCTGATCGCCGACGCCCCGATGATGGAC
TACGTCGGCCTCGAGCTGGCGGCCCGCTACTCCGACTACTCCCTCGCGGGCGAGGTGTGGACGTACAAGGCCGGCGGCGA
GTGGCGGGTGAACCCCGACATCCGCTTCCGCGCCCTCTACCAGCGCGCGGTGCGGGCGCCGAACATCACCGAGCTGTTCG
GCGGCCTGGTGAACACCTATCCGTTCATCGTCGACCCCTGCTCGGCCTCGGCCAACCCGTCGGGCCAGGTCGCGGCGCTC
TGCGTCGCCCAGGGCGTGCCGGCCTCGGCGCTGGGCAGCTACCAGCAGAACGGCGCGGCGCTCGAGGCGCGGCTGGTCTC
GAACCCCAACCTCGATCCCGAGAAGTCCGACACGCTGACGCTGGGCATGGTCTACACGCCCTCGCAGATCCCGGGCCTCA
GCCTGACGCTGGACTACTACGACATCGAGATCTCGGACGCGATCGAGCGTCTGGGCGGCGGCGCCCAGGGCGTGCTGGCC
GGGTGCTTCGCCTCGGGCGATGCGAACAACCCATTCTGCCGCACCTTCTCGCGCTCGCCGACGACCTACGAGATCGTCGA
CTTCGGCGTGCCGCTCGCCAACGTCGCCAGCCTGAAGACCTCGGGCGTCGACATGGCCGCGTCCTACCGCTGGTCGATGG
ACAGCCTGGGGATCGGCGACGACGACGCCGACCTGCGGGCGACGCTGATGGGCACCTGGGTCGACAAGAACGTCTTCCGC
GCCAACGACACCGCCGCGCCGACGGACCGGGTCGGGACGGTGGGCGGCGACACGCCGGCGATCCCGGAATGGCGCGCCAA
CGCGACCCTGAACTACGCCTCGGCCGGCCTCGAGCTGACCTGGAACGTCGAGTACCTGTCGTCGGTGAAGGACCGCAAGT
ACGCCAACAACCTGGCGGCCGGCGTGGCCAACCCGAAGGCGGGGATCGCCAATCCGAAGGTCGGCGCCTACTGGTACCAC
AACGTCCGGGCGGCCTACGACCTCGACCGGTTCACCGTCTACGGCGGGGTGCGGAACCTGTTCGACAAGGACCCGCCGAT
GCTGTCGTCGCCGATCGAGTCCAACACGGACCCGAACACCTACGACATGATCGGCCGCTACCTCTTCGTGGGCGTGAGCG
CCAAGTTCTAG

Upstream 100 bases:

>100_bases
ACCCCAAGCCGTACCACGAGGCCAGACCATGGGGGCGGCCGCGGCCGCCCCCGCATGACCTGACGACACCTACACACAAC
GAGAGAGAAGGGGGTTTTCT

Downstream 100 bases:

>100_bases
GCCCCACACAGGGACTTGACGCTCCATCGGGGGCCGGCTCGCGCCGGCCCCCTTTTTCTTGGCCTCAATCGGGCAAGGCG
GCCCTGGCGGGCGCGCCGGA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 936; Mature: 936

Protein sequence:

>936_residues
MLRSAWRGALAASTATAALLAVAPAAQAQDGEVAEVVVTGSRIARQDLVSSSPVATVSQEEIAVVGANNVEEYLNSLPQL
IAGATKSTNVAGSADATATLNLRGLGAKRTLVLVNGRRFVPTTQEGIVDVNNIPASLVERVEVVTGGASAVYGSDAMAGV
VNFILKDRFQGLEMDAQYGVSDEGDADAWNASATMGAAGEKASAWLHVSLEQRKKLRGTERPISVVSYIDANGNFVPSGS
AGRLGGTLVGIPTPNGSGGFVNRDYALDNMIPRPFVNSDFNFDARGDYAIQIPLDRVNVYGRGEYEFTPDISGFVEVTYN
NLDSRAILSPTAPNIRQTTNVPGLPANASFITPEIRALLNARPDPNAPFSLRFLAPEPFPKREIAFNRDIYRLLGGFEGR
LSDSWQWGVSYSYSHVATTEVQKGDISRRTFVEASTPSAADPTKCANGNPRCVLVTNLTNWSPELVDYLLVDNISSTEIT
DQVASAYATGDLFTLPAGEVKAAIGAEWRKISSVDNPAPVLLDFASAGFGERNRTAGSYSVKELFGEAIVPLIADAPMMD
YVGLELAARYSDYSLAGEVWTYKAGGEWRVNPDIRFRALYQRAVRAPNITELFGGLVNTYPFIVDPCSASANPSGQVAAL
CVAQGVPASALGSYQQNGAALEARLVSNPNLDPEKSDTLTLGMVYTPSQIPGLSLTLDYYDIEISDAIERLGGGAQGVLA
GCFASGDANNPFCRTFSRSPTTYEIVDFGVPLANVASLKTSGVDMAASYRWSMDSLGIGDDDADLRATLMGTWVDKNVFR
ANDTAAPTDRVGTVGGDTPAIPEWRANATLNYASAGLELTWNVEYLSSVKDRKYANNLAAGVANPKAGIANPKVGAYWYH
NVRAAYDLDRFTVYGGVRNLFDKDPPMLSSPIESNTDPNTYDMIGRYLFVGVSAKF

Sequences:

>Translated_936_residues
MLRSAWRGALAASTATAALLAVAPAAQAQDGEVAEVVVTGSRIARQDLVSSSPVATVSQEEIAVVGANNVEEYLNSLPQL
IAGATKSTNVAGSADATATLNLRGLGAKRTLVLVNGRRFVPTTQEGIVDVNNIPASLVERVEVVTGGASAVYGSDAMAGV
VNFILKDRFQGLEMDAQYGVSDEGDADAWNASATMGAAGEKASAWLHVSLEQRKKLRGTERPISVVSYIDANGNFVPSGS
AGRLGGTLVGIPTPNGSGGFVNRDYALDNMIPRPFVNSDFNFDARGDYAIQIPLDRVNVYGRGEYEFTPDISGFVEVTYN
NLDSRAILSPTAPNIRQTTNVPGLPANASFITPEIRALLNARPDPNAPFSLRFLAPEPFPKREIAFNRDIYRLLGGFEGR
LSDSWQWGVSYSYSHVATTEVQKGDISRRTFVEASTPSAADPTKCANGNPRCVLVTNLTNWSPELVDYLLVDNISSTEIT
DQVASAYATGDLFTLPAGEVKAAIGAEWRKISSVDNPAPVLLDFASAGFGERNRTAGSYSVKELFGEAIVPLIADAPMMD
YVGLELAARYSDYSLAGEVWTYKAGGEWRVNPDIRFRALYQRAVRAPNITELFGGLVNTYPFIVDPCSASANPSGQVAAL
CVAQGVPASALGSYQQNGAALEARLVSNPNLDPEKSDTLTLGMVYTPSQIPGLSLTLDYYDIEISDAIERLGGGAQGVLA
GCFASGDANNPFCRTFSRSPTTYEIVDFGVPLANVASLKTSGVDMAASYRWSMDSLGIGDDDADLRATLMGTWVDKNVFR
ANDTAAPTDRVGTVGGDTPAIPEWRANATLNYASAGLELTWNVEYLSSVKDRKYANNLAAGVANPKAGIANPKVGAYWYH
NVRAAYDLDRFTVYGGVRNLFDKDPPMLSSPIESNTDPNTYDMIGRYLFVGVSAKF
>Mature_936_residues
MLRSAWRGALAASTATAALLAVAPAAQAQDGEVAEVVVTGSRIARQDLVSSSPVATVSQEEIAVVGANNVEEYLNSLPQL
IAGATKSTNVAGSADATATLNLRGLGAKRTLVLVNGRRFVPTTQEGIVDVNNIPASLVERVEVVTGGASAVYGSDAMAGV
VNFILKDRFQGLEMDAQYGVSDEGDADAWNASATMGAAGEKASAWLHVSLEQRKKLRGTERPISVVSYIDANGNFVPSGS
AGRLGGTLVGIPTPNGSGGFVNRDYALDNMIPRPFVNSDFNFDARGDYAIQIPLDRVNVYGRGEYEFTPDISGFVEVTYN
NLDSRAILSPTAPNIRQTTNVPGLPANASFITPEIRALLNARPDPNAPFSLRFLAPEPFPKREIAFNRDIYRLLGGFEGR
LSDSWQWGVSYSYSHVATTEVQKGDISRRTFVEASTPSAADPTKCANGNPRCVLVTNLTNWSPELVDYLLVDNISSTEIT
DQVASAYATGDLFTLPAGEVKAAIGAEWRKISSVDNPAPVLLDFASAGFGERNRTAGSYSVKELFGEAIVPLIADAPMMD
YVGLELAARYSDYSLAGEVWTYKAGGEWRVNPDIRFRALYQRAVRAPNITELFGGLVNTYPFIVDPCSASANPSGQVAAL
CVAQGVPASALGSYQQNGAALEARLVSNPNLDPEKSDTLTLGMVYTPSQIPGLSLTLDYYDIEISDAIERLGGGAQGVLA
GCFASGDANNPFCRTFSRSPTTYEIVDFGVPLANVASLKTSGVDMAASYRWSMDSLGIGDDDADLRATLMGTWVDKNVFR
ANDTAAPTDRVGTVGGDTPAIPEWRANATLNYASAGLELTWNVEYLSSVKDRKYANNLAAGVANPKAGIANPKVGAYWYH
NVRAAYDLDRFTVYGGVRNLFDKDPPMLSSPIESNTDPNTYDMIGRYLFVGVSAKF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=255, Percent_Identity=30.5882352941176, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1788478, Length=161, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 99924; Mature: 99924

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSAWRGALAASTATAALLAVAPAAQAQDGEVAEVVVTGSRIARQDLVSSSPVATVSQE
CCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCEEECCC
EIAVVGANNVEEYLNSLPQLIAGATKSTNVAGSADATATLNLRGLGAKRTLVLVNGRRFV
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCEEC
PTTQEGIVDVNNIPASLVERVEVVTGGASAVYGSDAMAGVVNFILKDRFQGLEMDAQYGV
CCCHHCEEECCCCCHHHHHHHHHEECCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
SDEGDADAWNASATMGAAGEKASAWLHVSLEQRKKLRGTERPISVVSYIDANGNFVPSGS
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
AGRLGGTLVGIPTPNGSGGFVNRDYALDNMIPRPFVNSDFNFDARGDYAIQIPLDRVNVY
CCCCCCEEEEECCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHEEEE
GRGEYEFTPDISGFVEVTYNNLDSRAILSPTAPNIRQTTNVPGLPANASFITPEIRALLN
ECCCEEECCCCCCEEEEEECCCCCCEEECCCCCCCHHCCCCCCCCCCCCEECHHHHHHHC
ARPDPNAPFSLRFLAPEPFPKREIAFNRDIYRLLGGFEGRLSDSWQWGVSYSYSHVATTE
CCCCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHCCCCCCCCCCEEECCEECCCEEEECC
VQKGDISRRTFVEASTPSAADPTKCANGNPRCVLVTNLTNWSPELVDYLLVDNISSTEIT
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHH
DQVASAYATGDLFTLPAGEVKAAIGAEWRKISSVDNPAPVLLDFASAGFGERNRTAGSYS
HHHHHHHHCCCEEECCCCHHHHHHCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCH
VKELFGEAIVPLIADAPMMDYVGLELAARYSDYSLAGEVWTYKAGGEWRVNPDIRFRALY
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEECCCEEEEECCCEEEECCCHHHHHHH
QRAVRAPNITELFGGLVNTYPFIVDPCSASANPSGQVAALCVAQGVPASALGSYQQNGAA
HHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCHHCCCCE
LEARLVSNPNLDPEKSDTLTLGMVYTPSQIPGLSLTLDYYDIEISDAIERLGGGAQGVLA
EEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEHHHHHHHHCCCCCHHHE
GCFASGDANNPFCRTFSRSPTTYEIVDFGVPLANVASLKTSGVDMAASYRWSMDSLGIGD
EEECCCCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCC
DDADLRATLMGTWVDKNVFRANDTAAPTDRVGTVGGDTPAIPEWRANATLNYASAGLELT
CCCCEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEE
WNVEYLSSVKDRKYANNLAAGVANPKAGIANPKVGAYWYHNVRAAYDLDRFTVYGGVRNL
EEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCCEEEEHHHHHH
FDKDPPMLSSPIESNTDPNTYDMIGRYLFVGVSAKF
HCCCCCCCCCCCCCCCCCCHHHHHHCEEEEEEECCC
>Mature Secondary Structure
MLRSAWRGALAASTATAALLAVAPAAQAQDGEVAEVVVTGSRIARQDLVSSSPVATVSQE
CCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCEEECCC
EIAVVGANNVEEYLNSLPQLIAGATKSTNVAGSADATATLNLRGLGAKRTLVLVNGRRFV
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCEEC
PTTQEGIVDVNNIPASLVERVEVVTGGASAVYGSDAMAGVVNFILKDRFQGLEMDAQYGV
CCCHHCEEECCCCCHHHHHHHHHEECCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
SDEGDADAWNASATMGAAGEKASAWLHVSLEQRKKLRGTERPISVVSYIDANGNFVPSGS
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
AGRLGGTLVGIPTPNGSGGFVNRDYALDNMIPRPFVNSDFNFDARGDYAIQIPLDRVNVY
CCCCCCEEEEECCCCCCCCCEECCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHEEEE
GRGEYEFTPDISGFVEVTYNNLDSRAILSPTAPNIRQTTNVPGLPANASFITPEIRALLN
ECCCEEECCCCCCEEEEEECCCCCCEEECCCCCCCHHCCCCCCCCCCCCEECHHHHHHHC
ARPDPNAPFSLRFLAPEPFPKREIAFNRDIYRLLGGFEGRLSDSWQWGVSYSYSHVATTE
CCCCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHCCCCCCCCCCEEECCEECCCEEEECC
VQKGDISRRTFVEASTPSAADPTKCANGNPRCVLVTNLTNWSPELVDYLLVDNISSTEIT
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHH
DQVASAYATGDLFTLPAGEVKAAIGAEWRKISSVDNPAPVLLDFASAGFGERNRTAGSYS
HHHHHHHHCCCEEECCCCHHHHHHCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCH
VKELFGEAIVPLIADAPMMDYVGLELAARYSDYSLAGEVWTYKAGGEWRVNPDIRFRALY
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEECCCEEEEECCCEEEECCCHHHHHHH
QRAVRAPNITELFGGLVNTYPFIVDPCSASANPSGQVAALCVAQGVPASALGSYQQNGAA
HHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCHHCCCCE
LEARLVSNPNLDPEKSDTLTLGMVYTPSQIPGLSLTLDYYDIEISDAIERLGGGAQGVLA
EEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEHHHHHHHHCCCCCHHHE
GCFASGDANNPFCRTFSRSPTTYEIVDFGVPLANVASLKTSGVDMAASYRWSMDSLGIGD
EEECCCCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCC
DDADLRATLMGTWVDKNVFRANDTAAPTDRVGTVGGDTPAIPEWRANATLNYASAGLELT
CCCCEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEE
WNVEYLSSVKDRKYANNLAAGVANPKAGIANPKVGAYWYHNVRAAYDLDRFTVYGGVRNL
EEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEEECCCCEEEEHHHHHH
FDKDPPMLSSPIESNTDPNTYDMIGRYLFVGVSAKF
HCCCCCCCCCCCCCCCCCCHHHHHHCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA