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The map label for this gene is puuB [H]

Identifier: 197105456

GI number: 197105456

Start: 2226892

End: 2228274

Strand: Reverse

Name: puuB [H]

Synonym: PHZ_c1993

Alternate gene names: 197105456

Gene position: 2228274-2226892 (Counterclockwise)

Preceding gene: 197105457

Following gene: 197105455

Centisome position: 55.76

GC content: 73.17

Gene sequence:

>1383_bases
GTGACCTATGAGGGCCTGAGCTACTGGCTGGAGACCGTTGGCGAACCGCTGACTCCCCGCCCGGCGCTGACCGAGGACCT
GACGGTCGACGTCGCCATCGTCGGGGCCGGCTATTCGGGCCTGTGGACCGCCTACTATCTCCTGTCCAAGGAGCCGGGCC
TCAGCGTCGCCGTCCTCGAGGCGGAGATCGCCGGATACGGCGCCTCCGGCCGCAACGGCGGCTGGTGCTCGTCCCGCTTC
CCCGTGGATCCCGGCGTCCTTGAGCGCCGCTACGGAGCCGAGGTCGCCCGCGCCACGATCGAGGCGAGCGTGGGGGCGGT
CGCCGAGGTGGGCCGCGTCTGCGAGGCCGAGGGCATCGACGCCGACTTCCGCATCAACGGGATTCTCAGCTTCGCCCAGG
GCGAGGCCCAGATGGCCAGCGTGCGCGCGACCCATGCCGCCTATGAGCGCCTGGGCCTCGGAGAGAAGCATCGCCTGCTC
GACGGCCAGGCGGCCCGCGCCCGGGTGAGCGTCCCGGGAATGGCGGGCGGCCTGTTCACGCCGTTCAGCGGCGCCGTCCA
CCCCGCCAAGCTGGCCCGCGGGCTGGCCCGCGCGGTCGAGCGCCGCGGCGGCGTGATCTTCGAACGGACGCGGGTCCGGC
GCGTCGAACCCGGCCAGACGCCGAGGGCGGTCACGGAGGGGGGCGTGGTCACGGCGCGGCGCGCGGTGGTGCTCGCCGCC
GAGGCCTACCTGCCGAAGCTTCCCGGCTGGGAGCGGGCGGTCCTGCCGATGTCGTCGACGATCGTCATGACCAAGCCCCT
GTCCGCCGCACAGTGGGACGCGATCGGCTGGCAAGGGGGCGAGGGCATCGGCTCGCAGGCCTTCACGGTGAACTACTTCA
CCAAGACGCCGGACGGCCGGATCCTCTACGGCAGCCGGGGCGCGCCCTACGTCTACGGGTCGGCCACGGAGGGCGGCGAG
GCCCAGCTGCGGCGGACCGAGGAGAAGATGCGCCACGAGCTGCGCGGGTTCTTCCCGGTGCTCGACGAGGACTCGTTCTC
CCACGCCTGGGGCGGTCACCTCGGCGTCACACGCGACTGGACGCCCTCGGTCGATTTCGACCCGGAGCAGCGGATCGCCT
GCCTCTACGGCTACACGGGGCGTGGGGTCTCGACCACGAACCTCAGCGCCCGGCTCCTCTCGGACATCCTGCTGGGGCGG
GACAGCGACCTGCGGACCTTGCCGATCGCCTCGCGCCGCTCGCGTCGCTGGGAGCCGGAGCCGTTCCGCTGGATGGGGGT
CCGCTACGTCCAGGACGCCTTCCGGCGCATGGACGAGGCGCGGGACGGCGGCCGGCCGATGCCGCTCGACGCGTCCCTCG
CGCGGCGCCTCGGCTCGCAGTAG

Upstream 100 bases:

>100_bases
GGGTCAGAGCGTCTATTTCCCGGCCAATACCGGCGGCGTGTGGCGCGTCGAGGAAACGCTGCGGAAGATCTACATCATCT
TCGACGAGAAGGCCTGACTC

Downstream 100 bases:

>100_bases
GACGGGCCCCGCCGCGCTGGACCGCGCCGGCCGGCGCGTGGCATGTCCGCGTCGAACGGGGGGATCAGGCGTTTGGATGG
CCGTCCGCAATCTTCCGCAG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTYEGLSYWLETVGEPLTPRPALTEDLTVDVAIVGAGYSGLWTAYYLLSKEPGLSVAVLEAEIAGYGASGRNGGWCSSRF
PVDPGVLERRYGAEVARATIEASVGAVAEVGRVCEAEGIDADFRINGILSFAQGEAQMASVRATHAAYERLGLGEKHRLL
DGQAARARVSVPGMAGGLFTPFSGAVHPAKLARGLARAVERRGGVIFERTRVRRVEPGQTPRAVTEGGVVTARRAVVLAA
EAYLPKLPGWERAVLPMSSTIVMTKPLSAAQWDAIGWQGGEGIGSQAFTVNYFTKTPDGRILYGSRGAPYVYGSATEGGE
AQLRRTEEKMRHELRGFFPVLDEDSFSHAWGGHLGVTRDWTPSVDFDPEQRIACLYGYTGRGVSTTNLSARLLSDILLGR
DSDLRTLPIASRRSRRWEPEPFRWMGVRYVQDAFRRMDEARDGGRPMPLDASLARRLGSQ

Sequences:

>Translated_460_residues
MTYEGLSYWLETVGEPLTPRPALTEDLTVDVAIVGAGYSGLWTAYYLLSKEPGLSVAVLEAEIAGYGASGRNGGWCSSRF
PVDPGVLERRYGAEVARATIEASVGAVAEVGRVCEAEGIDADFRINGILSFAQGEAQMASVRATHAAYERLGLGEKHRLL
DGQAARARVSVPGMAGGLFTPFSGAVHPAKLARGLARAVERRGGVIFERTRVRRVEPGQTPRAVTEGGVVTARRAVVLAA
EAYLPKLPGWERAVLPMSSTIVMTKPLSAAQWDAIGWQGGEGIGSQAFTVNYFTKTPDGRILYGSRGAPYVYGSATEGGE
AQLRRTEEKMRHELRGFFPVLDEDSFSHAWGGHLGVTRDWTPSVDFDPEQRIACLYGYTGRGVSTTNLSARLLSDILLGR
DSDLRTLPIASRRSRRWEPEPFRWMGVRYVQDAFRRMDEARDGGRPMPLDASLARRLGSQ
>Mature_459_residues
TYEGLSYWLETVGEPLTPRPALTEDLTVDVAIVGAGYSGLWTAYYLLSKEPGLSVAVLEAEIAGYGASGRNGGWCSSRFP
VDPGVLERRYGAEVARATIEASVGAVAEVGRVCEAEGIDADFRINGILSFAQGEAQMASVRATHAAYERLGLGEKHRLLD
GQAARARVSVPGMAGGLFTPFSGAVHPAKLARGLARAVERRGGVIFERTRVRRVEPGQTPRAVTEGGVVTARRAVVLAAE
AYLPKLPGWERAVLPMSSTIVMTKPLSAAQWDAIGWQGGEGIGSQAFTVNYFTKTPDGRILYGSRGAPYVYGSATEGGEA
QLRRTEEKMRHELRGFFPVLDEDSFSHAWGGHLGVTRDWTPSVDFDPEQRIACLYGYTGRGVSTTNLSARLLSDILLGRD
SDLRTLPIASRRSRRWEPEPFRWMGVRYVQDAFRRMDEARDGGRPMPLDASLARRLGSQ

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=435, Percent_Identity=29.8850574712644, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 50010; Mature: 49879

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYEGLSYWLETVGEPLTPRPALTEDLTVDVAIVGAGYSGLWTAYYLLSKEPGLSVAVLE
CCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHEEECCCCCEEEEEE
AEIAGYGASGRNGGWCSSRFPVDPGVLERRYGAEVARATIEASVGAVAEVGRVCEAEGID
EEHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ADFRINGILSFAQGEAQMASVRATHAAYERLGLGEKHRLLDGQAARARVSVPGMAGGLFT
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCC
PFSGAVHPAKLARGLARAVERRGGVIFERTRVRRVEPGQTPRAVTEGGVVTARRAVVLAA
CCCCCCCHHHHHHHHHHHHHHHCCEEEEECHHEECCCCCCCCHHCCCCEEEEHHEEEEEH
EAYLPKLPGWERAVLPMSSTIVMTKPLSAAQWDAIGWQGGEGIGSQAFTVNYFTKTPDGR
HHHCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCE
ILYGSRGAPYVYGSATEGGEAQLRRTEEKMRHELRGFFPVLDEDSFSHAWGGHLGVTRDW
EEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TPSVDFDPEQRIACLYGYTGRGVSTTNLSARLLSDILLGRDSDLRTLPIASRRSRRWEPE
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCHHHCCCCCCC
PFRWMGVRYVQDAFRRMDEARDGGRPMPLDASLARRLGSQ
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TYEGLSYWLETVGEPLTPRPALTEDLTVDVAIVGAGYSGLWTAYYLLSKEPGLSVAVLE
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHEEECCCCCEEEEEE
AEIAGYGASGRNGGWCSSRFPVDPGVLERRYGAEVARATIEASVGAVAEVGRVCEAEGID
EEHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ADFRINGILSFAQGEAQMASVRATHAAYERLGLGEKHRLLDGQAARARVSVPGMAGGLFT
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCC
PFSGAVHPAKLARGLARAVERRGGVIFERTRVRRVEPGQTPRAVTEGGVVTARRAVVLAA
CCCCCCCHHHHHHHHHHHHHHHCCEEEEECHHEECCCCCCCCHHCCCCEEEEHHEEEEEH
EAYLPKLPGWERAVLPMSSTIVMTKPLSAAQWDAIGWQGGEGIGSQAFTVNYFTKTPDGR
HHHCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCE
ILYGSRGAPYVYGSATEGGEAQLRRTEEKMRHELRGFFPVLDEDSFSHAWGGHLGVTRDW
EEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TPSVDFDPEQRIACLYGYTGRGVSTTNLSARLLSDILLGRDSDLRTLPIASRRSRRWEPE
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCHHHCCCCCCC
PFRWMGVRYVQDAFRRMDEARDGGRPMPLDASLARRLGSQ
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]