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The map label for this gene is nadE [H]

Identifier: 197105394

GI number: 197105394

Start: 2164796

End: 2166841

Strand: Reverse

Name: nadE [H]

Synonym: PHZ_c1931

Alternate gene names: 197105394

Gene position: 2166841-2164796 (Counterclockwise)

Preceding gene: 197105395

Following gene: 197105393

Centisome position: 54.22

GC content: 69.5

Gene sequence:

>2046_bases
GTGACGAAGTCGTTCTACGCCATCCACAGCCACGGCTTCGTCCGCGTCGCGGCCTGCACGCCGCGCGTCGCGGTAGGCGA
TCCCGGGTTCAACGCCGCCGAGACGCTCCGCATGGCGCACGAGGGCGACGCCCGCGGCTGCGACCTGATGCTGTTCCCGG
AGCTGGGGATCAGCGCCTACGCCATCGACGACCTGCTGCTGCAGGACGCCCTGCTGAAGCGGGTGGAGGACGAAATCGCG
GCCCTCGCCGCCGCCAGCGCCAAGCTCTCGCCCGTGCTGGTGGTGGGCGCGCCCGTGGCCCGCAACGGCCGGCTCTACAA
CTGCGCCGTGGCGATCGCCCGCGGGCGTATCCTGGGCGTCGTGCCCAAGAGCTATCTGCCCAACTACCGCGAGTACTACG
AGAAGCGCTGGTTCGCGCCGGGCCACGGGGTCGAGGGACTGGAGGTCGAACTGGCCGGCCAGGCGGCGCCCTTCGGGACC
GACCTGCTGTTCGAGGCGTCGGACCTGAAGGACTTCGTCTTCCACGCCGAGATCTGCGAGGACTTCTGGGCCCCGGCGCC
GCCCTCAACGGAAGGCGCGCTCGCCGGCGCGCTTATCCTCTGTAATCTGTCGGCCTCGAACATCGTGGTGGGCAAGGCGG
ACGAGCGGGCGCTGCTCTGCGCGTCGCAGTCGGCCCGCTGTTCGGCCGCCTACCTCTATTCGGCCGCGGGGCCGGGGGAG
AGCACGACCGACCTGGCCTGGGACGGCCAGGCCTCGATCCACGAGCTCGGCCGCGAGCTGGCCTGCACCGACCGCTTCCC
GACCGAGGCCCAGATGGCGGTGGCCGACATCGACGTCGAGAAGCTGCGGCTGGAGCGGATGCGCTCGCCCACGTTCAACG
ACGCGGCGATCGCCCGCGGGCATCCCGAGCGCCGCTTCCGGCGGGTGCGCTTTGAGCACCGCCCGCACGGCAAGGATGTC
GGCCTGCTGCGGCCGGTCGACCGATTCCCCTTCGTGCCGGACGACCCCGCCCGGCTCGACAAGGACTGCTACGAGGCCTT
CAACATCCAGGTGCAAGGCCTTGTAAAGCGGCTGGAATCCACGAAGTCGCAGCACATCGTCATAGGCGTGTCAGGCGGCC
TGGATTCCACCCACGCCCTGATCGTGGCGGCCAAGGCCTTCGACCGGCTGGGCAAGCCGCGGTCCGACATCCTGGGCTTC
ACCATGCCGGGGTTCGCCACCAGCGAGGGGACGAAGTCCAACGCCTGGAAGCTGATGCGGGCGCTGGGCGTCACCGCCGA
GGAGATCGACATCCGGCCGGCGGCGACCCAGCTCCTGAAGGACATGGGCCATCCGTTCGCCGCGGGCGAGCCGGTCTATG
ACGTGACGTTCGAGAACGTGCAGGCGGGCCTTCGCACCGACTACCTGTTCCGCCTGGCCAACCAGCGCCACGGGTTCGTC
CTGGGCACGGGCGACCTGTCCGAACTGGCGCTGGGGTGGTGCACCTACGGCGTCGGCGACCAGATGAGCCACTACAACGT
CAACGGCTCGGTGGCGAAGACGCTGATCCAGCACCTGATCCGCTGGGTGGCCAAGACCGGCCAGTTCGACACGGCCGCGT
CCGAGACCCTGCTGTCGGTGCTGGACACCGAGATCTCTCCCGAGCTGGTGCCCGCCGACGCCAGCGGCGCGATCCAGAGC
ACCCAGGCCAAGATCGGCCCGTACGAGCTGCAGGACTTCAACGTCTACTGGATCACCCGCTACGGGCTTCGGCCGTCGAA
AGTGGCCTTCCTGGCCTGGCACGCCTGGCGCGACGTGGCGGAGGGCAGCTGGCCGCCGCACTTCCCGGCCGAGGCCCGCA
ACGCCTACGACCTGGCCACGATCAAAAAGTGGCTGTCGCTGTTCCTGTTCCGGTTCTTCGAGATCAGCCAGTTCAAGCGC
TCGGCCATGCCCAACGGGCCGAAGGTGATCTCGGGCGGCAATCTCAGTCCGCGCGGCGACTGGCGCGCGCCGTCCGACGG
CGTGGCGAGGGTCTGGCTCGAGGAGCTCGAGGCCAACGTGCCCTAG

Upstream 100 bases:

>100_bases
GAAGGGATGAAGTACGGCAAGGTCTAGGTTTCCCAGGCCGATCGCCCACGCTATATATGCTCAAAGTGAGCATTTCTCTC
GGACCGGAGACGGAGCCGTC

Downstream 100 bases:

>100_bases
CGCACCCTAGCGCGCCTGGGCGCCCGCGGGGCAGCAGGAGGAGGCGGCCGGGCGCCGGCGGGCGGCCATGCGCCGGATCA
ACGGCTCGATCCAGAACACC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MTKSFYAIHSHGFVRVAACTPRVAVGDPGFNAAETLRMAHEGDARGCDLMLFPELGISAYAIDDLLLQDALLKRVEDEIA
ALAAASAKLSPVLVVGAPVARNGRLYNCAVAIARGRILGVVPKSYLPNYREYYEKRWFAPGHGVEGLEVELAGQAAPFGT
DLLFEASDLKDFVFHAEICEDFWAPAPPSTEGALAGALILCNLSASNIVVGKADERALLCASQSARCSAAYLYSAAGPGE
STTDLAWDGQASIHELGRELACTDRFPTEAQMAVADIDVEKLRLERMRSPTFNDAAIARGHPERRFRRVRFEHRPHGKDV
GLLRPVDRFPFVPDDPARLDKDCYEAFNIQVQGLVKRLESTKSQHIVIGVSGGLDSTHALIVAAKAFDRLGKPRSDILGF
TMPGFATSEGTKSNAWKLMRALGVTAEEIDIRPAATQLLKDMGHPFAAGEPVYDVTFENVQAGLRTDYLFRLANQRHGFV
LGTGDLSELALGWCTYGVGDQMSHYNVNGSVAKTLIQHLIRWVAKTGQFDTAASETLLSVLDTEISPELVPADASGAIQS
TQAKIGPYELQDFNVYWITRYGLRPSKVAFLAWHAWRDVAEGSWPPHFPAEARNAYDLATIKKWLSLFLFRFFEISQFKR
SAMPNGPKVISGGNLSPRGDWRAPSDGVARVWLEELEANVP

Sequences:

>Translated_681_residues
MTKSFYAIHSHGFVRVAACTPRVAVGDPGFNAAETLRMAHEGDARGCDLMLFPELGISAYAIDDLLLQDALLKRVEDEIA
ALAAASAKLSPVLVVGAPVARNGRLYNCAVAIARGRILGVVPKSYLPNYREYYEKRWFAPGHGVEGLEVELAGQAAPFGT
DLLFEASDLKDFVFHAEICEDFWAPAPPSTEGALAGALILCNLSASNIVVGKADERALLCASQSARCSAAYLYSAAGPGE
STTDLAWDGQASIHELGRELACTDRFPTEAQMAVADIDVEKLRLERMRSPTFNDAAIARGHPERRFRRVRFEHRPHGKDV
GLLRPVDRFPFVPDDPARLDKDCYEAFNIQVQGLVKRLESTKSQHIVIGVSGGLDSTHALIVAAKAFDRLGKPRSDILGF
TMPGFATSEGTKSNAWKLMRALGVTAEEIDIRPAATQLLKDMGHPFAAGEPVYDVTFENVQAGLRTDYLFRLANQRHGFV
LGTGDLSELALGWCTYGVGDQMSHYNVNGSVAKTLIQHLIRWVAKTGQFDTAASETLLSVLDTEISPELVPADASGAIQS
TQAKIGPYELQDFNVYWITRYGLRPSKVAFLAWHAWRDVAEGSWPPHFPAEARNAYDLATIKKWLSLFLFRFFEISQFKR
SAMPNGPKVISGGNLSPRGDWRAPSDGVARVWLEELEANVP
>Mature_680_residues
TKSFYAIHSHGFVRVAACTPRVAVGDPGFNAAETLRMAHEGDARGCDLMLFPELGISAYAIDDLLLQDALLKRVEDEIAA
LAAASAKLSPVLVVGAPVARNGRLYNCAVAIARGRILGVVPKSYLPNYREYYEKRWFAPGHGVEGLEVELAGQAAPFGTD
LLFEASDLKDFVFHAEICEDFWAPAPPSTEGALAGALILCNLSASNIVVGKADERALLCASQSARCSAAYLYSAAGPGES
TTDLAWDGQASIHELGRELACTDRFPTEAQMAVADIDVEKLRLERMRSPTFNDAAIARGHPERRFRRVRFEHRPHGKDVG
LLRPVDRFPFVPDDPARLDKDCYEAFNIQVQGLVKRLESTKSQHIVIGVSGGLDSTHALIVAAKAFDRLGKPRSDILGFT
MPGFATSEGTKSNAWKLMRALGVTAEEIDIRPAATQLLKDMGHPFAAGEPVYDVTFENVQAGLRTDYLFRLANQRHGFVL
GTGDLSELALGWCTYGVGDQMSHYNVNGSVAKTLIQHLIRWVAKTGQFDTAASETLLSVLDTEISPELVPADASGAIQST
QAKIGPYELQDFNVYWITRYGLRPSKVAFLAWHAWRDVAEGSWPPHFPAEARNAYDLATIKKWLSLFLFRFFEISQFKRS
AMPNGPKVISGGNLSPRGDWRAPSDGVARVWLEELEANVP

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=621, Percent_Identity=25.2818035426731, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538756, Length=272, Percent_Identity=30.5147058823529, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321865, Length=290, Percent_Identity=27.5862068965517, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24641841, Length=595, Percent_Identity=23.1932773109244, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641843, Length=595, Percent_Identity=23.1932773109244, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 74534; Mature: 74403

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSFYAIHSHGFVRVAACTPRVAVGDPGFNAAETLRMAHEGDARGCDLMLFPELGISAY
CCCCEEEEECCCEEEEEECCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
AIDDLLLQDALLKRVEDEIAALAAASAKLSPVLVVGAPVARNGRLYNCAVAIARGRILGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEHHCCCEEEE
VPKSYLPNYREYYEKRWFAPGHGVEGLEVELAGQAAPFGTDLLFEASDLKDFVFHAEICE
CCHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHH
DFWAPAPPSTEGALAGALILCNLSASNIVVGKADERALLCASQSARCSAAYLYSAAGPGE
HHCCCCCCCCCCHHEEEEEEEECCCCCEEEECCCCCEEEECCCCCCCCEEEEEECCCCCC
STTDLAWDGQASIHELGRELACTDRFPTEAQMAVADIDVEKLRLERMRSPTFNDAAIARG
CCCCEEECCCHHHHHHCHHHCCCCCCCCCHHEEEECCCHHHHHHHHHCCCCCCCCHHHCC
HPERRFRRVRFEHRPHGKDVGLLRPVDRFPFVPDDPARLDKDCYEAFNIQVQGLVKRLES
CHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCHHHHCHHHHHHHCCHHHHHHHHHHC
TKSQHIVIGVSGGLDSTHALIVAAKAFDRLGKPRSDILGFTMPGFATSEGTKSNAWKLMR
CCCCEEEEEECCCCCCCHHHEEEHHHHHHHCCCHHHHCCEECCCCCCCCCCCHHHHHHHH
ALGVTAEEIDIRPAATQLLKDMGHPFAAGEPVYDVTFENVQAGLRTDYLFRLANQRHGFV
HHCCCHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCHHHHHHHHHHCCCCCEE
LGTGDLSELALGWCTYGVGDQMSHYNVNGSVAKTLIQHLIRWVAKTGQFDTAASETLLSV
EECCCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LDTEISPELVPADASGAIQSTQAKIGPYELQDFNVYWITRYGLRPSKVAFLAWHAWRDVA
HHCCCCCCEECCCCCCCCHHHHCCCCCEEEEECEEEEEEECCCCCCCEEEEEHHHHHHHH
EGSWPPHFPAEARNAYDLATIKKWLSLFLFRFFEISQFKRSAMPNGPKVISGGNLSPRGD
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
WRAPSDGVARVWLEELEANVP
CCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKSFYAIHSHGFVRVAACTPRVAVGDPGFNAAETLRMAHEGDARGCDLMLFPELGISAY
CCCEEEEECCCEEEEEECCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
AIDDLLLQDALLKRVEDEIAALAAASAKLSPVLVVGAPVARNGRLYNCAVAIARGRILGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEEHHCCCEEEE
VPKSYLPNYREYYEKRWFAPGHGVEGLEVELAGQAAPFGTDLLFEASDLKDFVFHAEICE
CCHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHH
DFWAPAPPSTEGALAGALILCNLSASNIVVGKADERALLCASQSARCSAAYLYSAAGPGE
HHCCCCCCCCCCHHEEEEEEEECCCCCEEEECCCCCEEEECCCCCCCCEEEEEECCCCCC
STTDLAWDGQASIHELGRELACTDRFPTEAQMAVADIDVEKLRLERMRSPTFNDAAIARG
CCCCEEECCCHHHHHHCHHHCCCCCCCCCHHEEEECCCHHHHHHHHHCCCCCCCCHHHCC
HPERRFRRVRFEHRPHGKDVGLLRPVDRFPFVPDDPARLDKDCYEAFNIQVQGLVKRLES
CHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCHHHHCHHHHHHHCCHHHHHHHHHHC
TKSQHIVIGVSGGLDSTHALIVAAKAFDRLGKPRSDILGFTMPGFATSEGTKSNAWKLMR
CCCCEEEEEECCCCCCCHHHEEEHHHHHHHCCCHHHHCCEECCCCCCCCCCCHHHHHHHH
ALGVTAEEIDIRPAATQLLKDMGHPFAAGEPVYDVTFENVQAGLRTDYLFRLANQRHGFV
HHCCCHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCHHHHHHHHHHCCCCCEE
LGTGDLSELALGWCTYGVGDQMSHYNVNGSVAKTLIQHLIRWVAKTGQFDTAASETLLSV
EECCCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LDTEISPELVPADASGAIQSTQAKIGPYELQDFNVYWITRYGLRPSKVAFLAWHAWRDVA
HHCCCCCCEECCCCCCCCHHHHCCCCCEEEEECEEEEEEECCCCCCCEEEEEHHHHHHHH
EGSWPPHFPAEARNAYDLATIKKWLSLFLFRFFEISQFKRSAMPNGPKVISGGNLSPRGD
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
WRAPSDGVARVWLEELEANVP
CCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]