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The map label for this gene is aspS

Identifier: 197105320

GI number: 197105320

Start: 2080316

End: 2082094

Strand: Reverse

Name: aspS

Synonym: PHZ_c1857

Alternate gene names: 197105320

Gene position: 2082094-2080316 (Counterclockwise)

Preceding gene: 197105323

Following gene: 197105317

Centisome position: 52.1

GC content: 68.24

Gene sequence:

>1779_bases
ATGCACCTCTACCGCACCCACACCTGCGGCCAACTCCGCGCTTCCGACACGGGCTCGAAGGTCCGCCTCTCGGGCTGGGT
CCACCGCAAGCGCGACCACGGCGGCCTGATCTTCATCGACCTGCGCGACCACTACGGGCTGACCCAGCTCGTGGTGAGCC
CATCGACGCCGGGATTCGACCTCGTCGAGCACGTCCGCGCCGAGAGCGTGATCCGCGTGGACGGCGAGGTGGTGGCCCGC
TCGGCCGAAACGGTGAACCCCAACCTGCCGACCGGCGAGATCGAGGTCGTGGTCCGTCAGGTTGAGGTGCTGTCGGAAGC
CGCCGAGCTGCCGCTGCCGGTCTTCGGCGAGCCGGAGTACCCCGAGGACATCCGGCTGAAGTACCGCTACCTCGACCTGC
GCCGCGAGACGCTGCACCGGAACATCGTGCTGCGCAGCCAGGTGATCCAGTCGATCCGCCGCCGGATGATCGACCAGGGC
TTCCTGGAGTTCCAGACGCCGATCCTGACGGCGAGCTCGCCCGAGGGCGCGCGCGACTTCCTCGTGCCCTCGCGCCTGCA
CCCGACCAAGTTCTACGCGCTGCCCCAGGCGCCCCAGCAGTTCAAGCAGCTGCTGATGGTCTCGGGCTTCGACCGCTACT
TCCAGATCGCGCCCTGCTTCCGCGACGAGGACCTGCGGGCCGACCGCAGCCTCGAGTTCTATCAGCTCGACGTGGAGATG
AGCTTCGTCACCCAGGACGACGTCTTCGCGGCCATCGAGCCGGTGATGCACGGCGTCTTCACCGAGTTCGGCGAGGGCAA
GCCGGTCACCCCGTACCCGTTCCCGCGCATCCCCTATCGCGAGGCGATCGCCAAGTACGGCTCGGACAAGCCCGACCTGC
GCAACCCGATCGAGATGCAGGACGTCTCCGAGCACTTCCGCGGCGGCGGCTTCGGCCTGTTCGCGCGGATCCTCGAGGAC
GCCAAGAACGCCGTCTGGGCGATCCCCGGCCCGAAGGGCGGCAGCCGCGCCTTCTGCGACCGCATGAACAGCTGGGCGCA
GGGCGAGGGCCAGCCGGGTCTCGGCTACATCTTCTGGTCCGACGACCAGGGCGGCTGGGGCGGGCCGATCGCCAAGAACC
TCGGTCCCGAGAAGACCGACGGCCTGATGAAGGCGCTGGGCCTGGGGCAGGGCGATGCGGCGTTCTTCGTCGCGGGCGAC
CCCAAGGTCTTCTACAAGTTCGCCGGCGCGGCCCGGACCAAGGTCGGGACGGACCTGAAGCTGATCGACGAAGGCCGCTT
CGAGTTCTGCTGGATCGTCGACTTCCCGATGTTCGAGTGGAGCGACGAAGAGAAGAAGTACGACTTCTCGCACAACCCCT
TCTCGATGCCGCAGGGCGAGCTGGACGCGCTCCTGAACAAGGAGCCGGGCGAGATCGTCGCCTACCAGTACGACATCGTC
TGCAACGGCCACGAGCTGTGCTCGGGCGCGATCCGGAACCATCGGCCGGACGTGATGCTCAAGGCCTTCGAGATCGCGGG
CTACGGCCCCGAGGTGGTCGAGGAGCAGTTCGGCGGCATGCTGAACGCGTTCCGGCACGGCGCGCCGCCGCACGGCGGCC
TGGCGCCCGGCATCGACCGGATCGTCATGCTGCTGGCCGGCGAGACGGCCATCCGCGAGGTCATCGCGTTCCCGCTGAAC
CAGCAGGGCCAGGACCTGCTGATGAACGCGCCGTCCGAGGTCTCCGAGAAGCAGCTCAAGGAGCTGCACATCCGGCTGGC
CCCGCCGATCAAGGCCTGA

Upstream 100 bases:

>100_bases
GGGTAGTCAAGCGCCCCCGGGACCTCAGCCGTGCTTGACAAGCCGGGATGCGCATGCGCCCTTCCGCGCCTCGCAAAACA
GCCGATTTCCGCAGCTTTTC

Downstream 100 bases:

>100_bases
GGCTAGGGGGCCTTACGGCTACTCGCCGATCGCCACGAAGTAGCGGGGCGCCTCCGGCGGCGCCGGGGGCTCCGGCGGAT
CGGGCGGCGGCGGCGTGCGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MHLYRTHTCGQLRASDTGSKVRLSGWVHRKRDHGGLIFIDLRDHYGLTQLVVSPSTPGFDLVEHVRAESVIRVDGEVVAR
SAETVNPNLPTGEIEVVVRQVEVLSEAAELPLPVFGEPEYPEDIRLKYRYLDLRRETLHRNIVLRSQVIQSIRRRMIDQG
FLEFQTPILTASSPEGARDFLVPSRLHPTKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADRSLEFYQLDVEM
SFVTQDDVFAAIEPVMHGVFTEFGEGKPVTPYPFPRIPYREAIAKYGSDKPDLRNPIEMQDVSEHFRGGGFGLFARILED
AKNAVWAIPGPKGGSRAFCDRMNSWAQGEGQPGLGYIFWSDDQGGWGGPIAKNLGPEKTDGLMKALGLGQGDAAFFVAGD
PKVFYKFAGAARTKVGTDLKLIDEGRFEFCWIVDFPMFEWSDEEKKYDFSHNPFSMPQGELDALLNKEPGEIVAYQYDIV
CNGHELCSGAIRNHRPDVMLKAFEIAGYGPEVVEEQFGGMLNAFRHGAPPHGGLAPGIDRIVMLLAGETAIREVIAFPLN
QQGQDLLMNAPSEVSEKQLKELHIRLAPPIKA

Sequences:

>Translated_592_residues
MHLYRTHTCGQLRASDTGSKVRLSGWVHRKRDHGGLIFIDLRDHYGLTQLVVSPSTPGFDLVEHVRAESVIRVDGEVVAR
SAETVNPNLPTGEIEVVVRQVEVLSEAAELPLPVFGEPEYPEDIRLKYRYLDLRRETLHRNIVLRSQVIQSIRRRMIDQG
FLEFQTPILTASSPEGARDFLVPSRLHPTKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADRSLEFYQLDVEM
SFVTQDDVFAAIEPVMHGVFTEFGEGKPVTPYPFPRIPYREAIAKYGSDKPDLRNPIEMQDVSEHFRGGGFGLFARILED
AKNAVWAIPGPKGGSRAFCDRMNSWAQGEGQPGLGYIFWSDDQGGWGGPIAKNLGPEKTDGLMKALGLGQGDAAFFVAGD
PKVFYKFAGAARTKVGTDLKLIDEGRFEFCWIVDFPMFEWSDEEKKYDFSHNPFSMPQGELDALLNKEPGEIVAYQYDIV
CNGHELCSGAIRNHRPDVMLKAFEIAGYGPEVVEEQFGGMLNAFRHGAPPHGGLAPGIDRIVMLLAGETAIREVIAFPLN
QQGQDLLMNAPSEVSEKQLKELHIRLAPPIKA
>Mature_592_residues
MHLYRTHTCGQLRASDTGSKVRLSGWVHRKRDHGGLIFIDLRDHYGLTQLVVSPSTPGFDLVEHVRAESVIRVDGEVVAR
SAETVNPNLPTGEIEVVVRQVEVLSEAAELPLPVFGEPEYPEDIRLKYRYLDLRRETLHRNIVLRSQVIQSIRRRMIDQG
FLEFQTPILTASSPEGARDFLVPSRLHPTKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADRSLEFYQLDVEM
SFVTQDDVFAAIEPVMHGVFTEFGEGKPVTPYPFPRIPYREAIAKYGSDKPDLRNPIEMQDVSEHFRGGGFGLFARILED
AKNAVWAIPGPKGGSRAFCDRMNSWAQGEGQPGLGYIFWSDDQGGWGGPIAKNLGPEKTDGLMKALGLGQGDAAFFVAGD
PKVFYKFAGAARTKVGTDLKLIDEGRFEFCWIVDFPMFEWSDEEKKYDFSHNPFSMPQGELDALLNKEPGEIVAYQYDIV
CNGHELCSGAIRNHRPDVMLKAFEIAGYGPEVVEEQFGGMLNAFRHGAPPHGGLAPGIDRIVMLLAGETAIREVIAFPLN
QQGQDLLMNAPSEVSEKQLKELHIRLAPPIKA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=602, Percent_Identity=36.8770764119601, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=318, Percent_Identity=26.7295597484277, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226491502, Length=247, Percent_Identity=25.1012145748988, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI5031815, Length=263, Percent_Identity=26.9961977186312, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194272210, Length=263, Percent_Identity=26.9961977186312, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=43.7074829931973, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1789256, Length=315, Percent_Identity=28.8888888888889, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1790571, Length=315, Percent_Identity=27.6190476190476, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=34.983498349835, Blast_Score=329, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI71984122, Length=302, Percent_Identity=26.8211920529801, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=336, Percent_Identity=25, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994340, Length=243, Percent_Identity=25.5144032921811, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=243, Percent_Identity=25.5144032921811, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=294, Percent_Identity=25.1700680272109, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=647, Percent_Identity=30.9119010819165, Blast_Score=249, Evalue=9e-67,
Organism=Saccharomyces cerevisiae, GI6321807, Length=296, Percent_Identity=25.6756756756757, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=251, Percent_Identity=28.2868525896414, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24584738, Length=610, Percent_Identity=35.2459016393443, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=29.045643153527, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24640849, Length=257, Percent_Identity=25.6809338521401, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24640851, Length=257, Percent_Identity=25.6809338521401, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PHEZH (B4RCT1)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130697.1
- GeneID:   6791372
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1857
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66337; Mature: 66337

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLYRTHTCGQLRASDTGSKVRLSGWVHRKRDHGGLIFIDLRDHYGLTQLVVSPSTPGFD
CCEEECCCCCCCCCCCCCCEEEEECEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCHH
LVEHVRAESVIRVDGEVVARSAETVNPNLPTGEIEVVVRQVEVLSEAAELPLPVFGEPEY
HHHHHHHCCEEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDIRLKYRYLDLRRETLHRNIVLRSQVIQSIRRRMIDQGFLEFQTPILTASSPEGARDF
CHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCCCCHHC
LVPSRLHPTKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADRSLEFYQLDVEM
CCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCEEEEEEEEE
SFVTQDDVFAAIEPVMHGVFTEFGEGKPVTPYPFPRIPYREAIAKYGSDKPDLRNPIEMQ
EEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVSEHFRGGGFGLFARILEDAKNAVWAIPGPKGGSRAFCDRMNSWAQGEGQPGLGYIFWS
HHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCHHCCCCCCCCEEEEEEC
DDQGGWGGPIAKNLGPEKTDGLMKALGLGQGDAAFFVAGDPKVFYKFAGAARTKVGTDLK
CCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHHCCCCCEE
LIDEGRFEFCWIVDFPMFEWSDEEKKYDFSHNPFSMPQGELDALLNKEPGEIVAYQYDIV
EEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEE
CNGHELCSGAIRNHRPDVMLKAFEIAGYGPEVVEEQFGGMLNAFRHGAPPHGGLAPGIDR
ECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLAGETAIREVIAFPLNQQGQDLLMNAPSEVSEKQLKELHIRLAPPIKA
HHHHHCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MHLYRTHTCGQLRASDTGSKVRLSGWVHRKRDHGGLIFIDLRDHYGLTQLVVSPSTPGFD
CCEEECCCCCCCCCCCCCCEEEEECEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCHH
LVEHVRAESVIRVDGEVVARSAETVNPNLPTGEIEVVVRQVEVLSEAAELPLPVFGEPEY
HHHHHHHCCEEEECHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDIRLKYRYLDLRRETLHRNIVLRSQVIQSIRRRMIDQGFLEFQTPILTASSPEGARDF
CHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEECCCCCCCHHC
LVPSRLHPTKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDLRADRSLEFYQLDVEM
CCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCEEEEEEEEE
SFVTQDDVFAAIEPVMHGVFTEFGEGKPVTPYPFPRIPYREAIAKYGSDKPDLRNPIEMQ
EEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVSEHFRGGGFGLFARILEDAKNAVWAIPGPKGGSRAFCDRMNSWAQGEGQPGLGYIFWS
HHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCHHCCCCCCCCEEEEEEC
DDQGGWGGPIAKNLGPEKTDGLMKALGLGQGDAAFFVAGDPKVFYKFAGAARTKVGTDLK
CCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCHHHCCCCCEE
LIDEGRFEFCWIVDFPMFEWSDEEKKYDFSHNPFSMPQGELDALLNKEPGEIVAYQYDIV
EEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEE
CNGHELCSGAIRNHRPDVMLKAFEIAGYGPEVVEEQFGGMLNAFRHGAPPHGGLAPGIDR
ECCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLAGETAIREVIAFPLNQQGQDLLMNAPSEVSEKQLKELHIRLAPPIKA
HHHHHCCCHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA