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The map label for this gene is thiG

Identifier: 197105310

GI number: 197105310

Start: 2071434

End: 2072222

Strand: Reverse

Name: thiG

Synonym: PHZ_c1847

Alternate gene names: 197105310

Gene position: 2072222-2071434 (Counterclockwise)

Preceding gene: 197105311

Following gene: 197105309

Centisome position: 51.85

GC content: 69.84

Gene sequence:

>789_bases
ATGGATGGGTCTGTCCGGAGCGAGGACACTTGGACGGTCGCCGGCCGCACGTTCCGTTCGCGCCTGATCGTCGGCACCGG
CAAGTACAAGGACTACGCCCAGAACGCCGCCGCGGCCGAGGCCGCCGGGGCCGAGATCGTCACCGTGGCGGTGCGGCGGG
TGAACCTTTCCGATCCCAGCCAGCCGATGCTGGTGGACCATGTGAAGCCCGACCGCTTCACCTTCCTGCCCAACACCGCG
GGCTGCTTCACCGGCGAGGACGCCATCCGCACCCTGCGCCTGGCGCGCGAGGCGGGGGGCTGGGACCTGGTGAAGTTGGA
GGTCCTGTCCAACACCAAGCATCTCCTGCCCGACATGGAGGAGACGCTGCGGGCGCTGAAGCTGCTGATCGCGGACGGCT
TCCAGGTGATGGTCTACTGCTCGGACGATCCGGTCTACGCCAAGAAGCTGGAGGAGGCGGGGGCCGCCGCCATCATGCCG
GCCGCCGCGCCGATCGGCTCGGGGCGGGGCATCCAGAACGCCCTGAACCTCGCGCTCATCATCGAGCAGGCCAAGGTGCC
GGTGCTGGTCGACGCGGGCGTCGGCACGGCCTCGGACGCCACGATCGCCATGGAGCTGGGCTGCGACGCGGTGCTGATGA
ACACCGCCATCGCCGAGGCCAAGGATCCGATCCGCATGGCCCGCGCCATGCGCCACGCCGTGATCGCCGGTCGCGAGGCC
TATCTGGCCGGCCGCATGCCCAAGCGGATGTACGCCGAGCCGTCCTCGCCGCTGTCGGGCCTGATCTGA

Upstream 100 bases:

>100_bases
GAGCGGAACCTGGAGATCGTGCCGAGGTCGGCCTATTCGGCCACGGCGCTCGCCGAGGGCGACCGGATCGAGATCGTGCA
TTTCATCGGAGGCGGCTGAC

Downstream 100 bases:

>100_bases
TGAGCCGCAAGTCGATCTTCATCACCGGCGCGGCCTCGGGCATGGGCCGCGAGACGGCGCTGCTGTTCGCCGAGAAGGGC
TGGTTCGTCGGCGCCTACGA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDGSVRSEDTWTVAGRTFRSRLIVGTGKYKDYAQNAAAAEAAGAEIVTVAVRRVNLSDPSQPMLVDHVKPDRFTFLPNTA
GCFTGEDAIRTLRLAREAGGWDLVKLEVLSNTKHLLPDMEETLRALKLLIADGFQVMVYCSDDPVYAKKLEEAGAAAIMP
AAAPIGSGRGIQNALNLALIIEQAKVPVLVDAGVGTASDATIAMELGCDAVLMNTAIAEAKDPIRMARAMRHAVIAGREA
YLAGRMPKRMYAEPSSPLSGLI

Sequences:

>Translated_262_residues
MDGSVRSEDTWTVAGRTFRSRLIVGTGKYKDYAQNAAAAEAAGAEIVTVAVRRVNLSDPSQPMLVDHVKPDRFTFLPNTA
GCFTGEDAIRTLRLAREAGGWDLVKLEVLSNTKHLLPDMEETLRALKLLIADGFQVMVYCSDDPVYAKKLEEAGAAAIMP
AAAPIGSGRGIQNALNLALIIEQAKVPVLVDAGVGTASDATIAMELGCDAVLMNTAIAEAKDPIRMARAMRHAVIAGREA
YLAGRMPKRMYAEPSSPLSGLI
>Mature_262_residues
MDGSVRSEDTWTVAGRTFRSRLIVGTGKYKDYAQNAAAAEAAGAEIVTVAVRRVNLSDPSQPMLVDHVKPDRFTFLPNTA
GCFTGEDAIRTLRLAREAGGWDLVKLEVLSNTKHLLPDMEETLRALKLLIADGFQVMVYCSDDPVYAKKLEEAGAAAIMP
AAAPIGSGRGIQNALNLALIIEQAKVPVLVDAGVGTASDATIAMELGCDAVLMNTAIAEAKDPIRMARAMRHAVIAGREA
YLAGRMPKRMYAEPSSPLSGLI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=250, Percent_Identity=46, Blast_Score=208, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_PHEZH (B4RCS1)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130687.1
- ProteinModelPortal:   B4RCS1
- GeneID:   6791362
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1847
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27954; Mature: 27954

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 105-105 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGSVRSEDTWTVAGRTFRSRLIVGTGKYKDYAQNAAAAEAAGAEIVTVAVRRVNLSDPS
CCCCCCCCCCEEEECHHEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
QPMLVDHVKPDRFTFLPNTAGCFTGEDAIRTLRLAREAGGWDLVKLEVLSNTKHLLPDME
CCEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCCCHH
ETLRALKLLIADGFQVMVYCSDDPVYAKKLEEAGAAAIMPAAAPIGSGRGIQNALNLALI
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHEEEE
IEQAKVPVLVDAGVGTASDATIAMELGCDAVLMNTAIAEAKDPIRMARAMRHAVIAGREA
EECCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
YLAGRMPKRMYAEPSSPLSGLI
HHHCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDGSVRSEDTWTVAGRTFRSRLIVGTGKYKDYAQNAAAAEAAGAEIVTVAVRRVNLSDPS
CCCCCCCCCCEEEECHHEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
QPMLVDHVKPDRFTFLPNTAGCFTGEDAIRTLRLAREAGGWDLVKLEVLSNTKHLLPDME
CCEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCCCHH
ETLRALKLLIADGFQVMVYCSDDPVYAKKLEEAGAAAIMPAAAPIGSGRGIQNALNLALI
HHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHEEEE
IEQAKVPVLVDAGVGTASDATIAMELGCDAVLMNTAIAEAKDPIRMARAMRHAVIAGREA
EECCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
YLAGRMPKRMYAEPSSPLSGLI
HHHCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA