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The map label for this gene is pyrG

Identifier: 197105214

GI number: 197105214

Start: 1959640

End: 1961280

Strand: Reverse

Name: pyrG

Synonym: PHZ_c1751

Alternate gene names: 197105214

Gene position: 1961280-1959640 (Counterclockwise)

Preceding gene: 197105215

Following gene: 197105212

Centisome position: 49.08

GC content: 67.89

Gene sequence:

>1641_bases
ATGGCCCGGTACATCTTCATCACCGGCGGCGTGGTCTCCTCTCTTGGTAAAGGTCTCGCGTCCGCCGCGCTTGGCGCCCT
GTTGCAGGCGCGCGGCTACAAGGTCCGTCTGAGGAAACTCGACCCCTATCTCAACGTCGATCCGGGGACGATGAGCCCCT
ACCAGCACGGCGAGGTCTTCGTGACCGACGACGGGGCCGAGACCGACCTCGACCTCGGCCACTACGAGCGCTTCACGGGC
GTCTCGGCCACCCGCGCCGACAACATCACGACGGGCCAGATCTACAAGACGATCATCGAGAAGGAGCGCCGCGGCGACTA
CCTCGGCGCGACGGTCCAGGTCATTCCGCACGTCACCGGCGAGATCAAGGACTTCGTCCTCTCCGATCCCGGCCCGGATG
TGGACTTCGTGCTGATCGAGGTCGGCGGCACGGTGGGCGACATCGAGGGCCTGCCGTTCTTCGAGGCGATCCGCCAGCTG
GGCCAGGAGCTGCCCCGCGGGCACGCCTGCTACATCCACCTGACGCTGCTGCCGTTCATCAAGACGGCCGGCGAGATGAA
GACCAAGCCGACCCAGCACTCGGTGAAGGAGCTGCGCTCGATCGGCATCCAGCCCGACATCCTGCTGTGCCGGACCGAGT
TCCCGATCCCCGAGGGCGAGCGGCGCAAGATCGCCCAGTTCTGCAACGTGCGCCCCAGCGCCGTCATCCAGGCGATGGAC
AGCGCCAACATCTACGCCGTGCCGTTGGACTATCACCACGAGGGACTCGACACCGAGGTGCTCGACGTCTTCGGCCTGCT
GGACCAGGCGCCGAAGCCTGACCTGACCCGCTGGGAAGAGATCTCCCACCGCCTGAGCCACCCCGACGGCGAGGTGAACA
TCGCCATCGTCGGCAAGTATACGGGCCTGACCGACGCCTACAAATCGCTGGTCGAGGCGCTGGTCCACGGCGGCGTGGCC
AACAACGTCCGGGTCAAGTTCGACTGGATCGAGGGCGAGGCCTTCGAGAAGGACGAGGACCTGATCGCCGTGCGTCTGGC
CAACGTCCACGGCGTGCTGGTCCCGGGGGCCTTCGGCGAGCGCGGCTCGGAGGGGATGATCCGGGCCGTCCAGTTCGCCC
GCGAGCACCAGATCCCGTACTTCGGCATCTGCTTCGGCATGCAGATGGCGATGATCGAGGCGGCCCGGAACCTGGCCGGC
ATCCGGCAAGCTTCGTCCACCGAGTTCGGCCCGACCTCCGAGCCCATCGTCGGCCTGATGACCGAGTGGACCCGGGGGAA
CGAGAAGCAGGTCCGCGCCGAGGGGGGCGAGCTGGGCGGCACCATGCGCTGCGGCGCCTATGACGCCGTGCTGGCCCCCG
GATCGCGGGTGGCCGAGATCTACGGCTCGACCGCCATCCAGGAGCGCCATCGCCACCGCTACGAGGTGAACATCGGCTAC
CGCGAGCGGATCGAGGCCACGGGCCTGAAGTTCACCGGCCTGTCGCCCGACGGCCAGCTGCCCGAGATCTGCGAGCGGGC
GGACCATCCCTGGTTCGTCGGCGTGCAGTACCACCCGGAGCTGAAGAGCCGGCCGTTCGACCCGCACCCGCTGTTCGCCA
GCTTCATCGGCGCGGCCAAGGAGCGGAGCCGGCTGGTCTAG

Upstream 100 bases:

>100_bases
TCCCCAAACGGCCCCCGCGCCCGCCGCGCCGCAGTAATCCGTGGATAGTTTGCACCACGGGGCGGGCCGCCCCGGCGAAT
CACGGCTACTCTGAGCGCCC

Downstream 100 bases:

>100_bases
GCCAGGCCCCTTTCGCGCAGCAGGGCCTCCTCGTCGCCCGACACCCAGCCGAAGATCCTGGGTCCCTCGTCGGTCAAGCG
TACGAAGTAGGTCACCTCGA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
VSATRADNITTGQIYKTIIEKERRGDYLGATVQVIPHVTGEIKDFVLSDPGPDVDFVLIEVGGTVGDIEGLPFFEAIRQL
GQELPRGHACYIHLTLLPFIKTAGEMKTKPTQHSVKELRSIGIQPDILLCRTEFPIPEGERRKIAQFCNVRPSAVIQAMD
SANIYAVPLDYHHEGLDTEVLDVFGLLDQAPKPDLTRWEEISHRLSHPDGEVNIAIVGKYTGLTDAYKSLVEALVHGGVA
NNVRVKFDWIEGEAFEKDEDLIAVRLANVHGVLVPGAFGERGSEGMIRAVQFAREHQIPYFGICFGMQMAMIEAARNLAG
IRQASSTEFGPTSEPIVGLMTEWTRGNEKQVRAEGGELGGTMRCGAYDAVLAPGSRVAEIYGSTAIQERHRHRYEVNIGY
RERIEATGLKFTGLSPDGQLPEICERADHPWFVGVQYHPELKSRPFDPHPLFASFIGAAKERSRLV

Sequences:

>Translated_546_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
VSATRADNITTGQIYKTIIEKERRGDYLGATVQVIPHVTGEIKDFVLSDPGPDVDFVLIEVGGTVGDIEGLPFFEAIRQL
GQELPRGHACYIHLTLLPFIKTAGEMKTKPTQHSVKELRSIGIQPDILLCRTEFPIPEGERRKIAQFCNVRPSAVIQAMD
SANIYAVPLDYHHEGLDTEVLDVFGLLDQAPKPDLTRWEEISHRLSHPDGEVNIAIVGKYTGLTDAYKSLVEALVHGGVA
NNVRVKFDWIEGEAFEKDEDLIAVRLANVHGVLVPGAFGERGSEGMIRAVQFAREHQIPYFGICFGMQMAMIEAARNLAG
IRQASSTEFGPTSEPIVGLMTEWTRGNEKQVRAEGGELGGTMRCGAYDAVLAPGSRVAEIYGSTAIQERHRHRYEVNIGY
RERIEATGLKFTGLSPDGQLPEICERADHPWFVGVQYHPELKSRPFDPHPLFASFIGAAKERSRLV
>Mature_545_residues
ARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGV
SATRADNITTGQIYKTIIEKERRGDYLGATVQVIPHVTGEIKDFVLSDPGPDVDFVLIEVGGTVGDIEGLPFFEAIRQLG
QELPRGHACYIHLTLLPFIKTAGEMKTKPTQHSVKELRSIGIQPDILLCRTEFPIPEGERRKIAQFCNVRPSAVIQAMDS
ANIYAVPLDYHHEGLDTEVLDVFGLLDQAPKPDLTRWEEISHRLSHPDGEVNIAIVGKYTGLTDAYKSLVEALVHGGVAN
NVRVKFDWIEGEAFEKDEDLIAVRLANVHGVLVPGAFGERGSEGMIRAVQFAREHQIPYFGICFGMQMAMIEAARNLAGI
RQASSTEFGPTSEPIVGLMTEWTRGNEKQVRAEGGELGGTMRCGAYDAVLAPGSRVAEIYGSTAIQERHRHRYEVNIGYR
ERIEATGLKFTGLSPDGQLPEICERADHPWFVGVQYHPELKSRPFDPHPLFASFIGAAKERSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=559, Percent_Identity=42.9338103756708, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI28559085, Length=556, Percent_Identity=42.2661870503597, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI28559083, Length=556, Percent_Identity=42.2661870503597, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI221316689, Length=556, Percent_Identity=42.2661870503597, Blast_Score=449, Evalue=1e-126,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=54.9815498154982, Blast_Score=569, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI25148299, Length=608, Percent_Identity=37.0065789473684, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=40.9490333919156, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=39.0158172231986, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24664469, Length=562, Percent_Identity=45.1957295373665, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21357815, Length=508, Percent_Identity=44.4881889763779, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_PHEZH (B4RBW5)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130591.1
- ProteinModelPortal:   B4RBW5
- GeneID:   6791264
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1751
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60178; Mature: 60047

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 384-384 ACT_SITE 518-518 ACT_SITE 520-520

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSATRADNITTGQIYKTIIEKERRGDYLGATVQVIPHVTG
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
EIKDFVLSDPGPDVDFVLIEVGGTVGDIEGLPFFEAIRQLGQELPRGHACYIHLTLLPFI
HHHHHEECCCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHH
KTAGEMKTKPTQHSVKELRSIGIQPDILLCRTEFPIPEGERRKIAQFCNVRPSAVIQAMD
HHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHC
SANIYAVPLDYHHEGLDTEVLDVFGLLDQAPKPDLTRWEEISHRLSHPDGEVNIAIVGKY
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEC
TGLTDAYKSLVEALVHGGVANNVRVKFDWIEGEAFEKDEDLIAVRLANVHGVLVPGAFGE
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCCC
RGSEGMIRAVQFAREHQIPYFGICFGMQMAMIEAARNLAGIRQASSTEFGPTSEPIVGLM
CCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
TEWTRGNEKQVRAEGGELGGTMRCGAYDAVLAPGSRVAEIYGSTAIQERHRHRYEVNIGY
HHHCCCCCCEEECCCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEEECCH
RERIEATGLKFTGLSPDGQLPEICERADHPWFVGVQYHPELKSRPFDPHPLFASFIGAAK
HHHHHHCCEEEECCCCCCCCHHHHHHCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHH
ERSRLV
HHHCCC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPYQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSATRADNITTGQIYKTIIEKERRGDYLGATVQVIPHVTG
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
EIKDFVLSDPGPDVDFVLIEVGGTVGDIEGLPFFEAIRQLGQELPRGHACYIHLTLLPFI
HHHHHEECCCCCCCEEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHH
KTAGEMKTKPTQHSVKELRSIGIQPDILLCRTEFPIPEGERRKIAQFCNVRPSAVIQAMD
HHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHC
SANIYAVPLDYHHEGLDTEVLDVFGLLDQAPKPDLTRWEEISHRLSHPDGEVNIAIVGKY
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEC
TGLTDAYKSLVEALVHGGVANNVRVKFDWIEGEAFEKDEDLIAVRLANVHGVLVPGAFGE
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCCCC
RGSEGMIRAVQFAREHQIPYFGICFGMQMAMIEAARNLAGIRQASSTEFGPTSEPIVGLM
CCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
TEWTRGNEKQVRAEGGELGGTMRCGAYDAVLAPGSRVAEIYGSTAIQERHRHRYEVNIGY
HHHCCCCCCEEECCCCCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEEECCH
RERIEATGLKFTGLSPDGQLPEICERADHPWFVGVQYHPELKSRPFDPHPLFASFIGAAK
HHHHHHCCEEEECCCCCCCCHHHHHHCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHH
ERSRLV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA