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The map label for this gene is eno

Identifier: 197105210

GI number: 197105210

Start: 1956637

End: 1957917

Strand: Reverse

Name: eno

Synonym: PHZ_c1747

Alternate gene names: 197105210

Gene position: 1957917-1956637 (Counterclockwise)

Preceding gene: 197105212

Following gene: 197105208

Centisome position: 48.99

GC content: 67.99

Gene sequence:

>1281_bases
ATGACCGAGATCGTCGACATCACCGCCCGTGAGATCCTGGACAGCCGGGGCAACCCGACCGTCGAGGTCGACGTGATCCT
CGAGGACGGCGCCATGGGCCGTGCGGCGGTGCCCTCGGGCGCCTCGACCGGGGCCCACGAGGCCGTGGAGAAGCGCGACG
GCGACAAGGCCCGCTACCTCGGCAAGGGCGTGCGGCAGGCCGTCGATGCGGTGAACGGCGAGATCTATGACGCGCTGTCA
GGCTCGGACGCCGAGGACCAGCGCCGCATCGACCGGATGCTGATCGAGCTGGACGGCACGCCGAACAAGAGCCGCCTGGG
CGCCAACGCCATCCTGGGCGTCAGCCTGGCCACGGCCAAGGCGGCGGCCAGCAGTTCGGCCCTGCCGCTCTACAAGTACG
TGGGCGGCGTCTCGGCCCGCATCCTGCCGGTGCCGATGATGAACATCATCAACGGCGGCGCCCACGCCGACAATCCGATC
GACATCCAGGAGTTCATGATCCTGCCGACCGGCGCCGAGACCTTCTCGGAAGCCCTGCGCATGGGCGCCGAGATCTTCCA
CGCGCTGAAGAAGCAGCTGAAGGACGCGGGCCACAACACCAACGTCGGGGACGAGGGCGGCTTCGCTCCCAACATCGGCT
CGGCCGAGGAGGCGCTGGCCTTCATCGTCAAGGCGGGCGAGGGCGCGGGCTACCGCTGCGGCGAGGACTTCCACCTGGGC
CTCGACGTGGCCTCCACCGAGTTCTTCAAGAACGGCAAGTACGTGATGGAGGGCGAGGGCAAGACGGTCGATCCCGCCGG
CATGGTCGAGTACCTGGCGGGCCTCGTCTCCAAGTTCCCGATCGTCACCATCGAGGACGGCTGTGCCGAGGACGACTTCG
ACGGCTGGAAGATCCTGACCGAGCGCCTGGGCGGCAAGGTCCAGCTCGTGGGCGACGACCTGTTCGTCACCAACCCGGAG
CGCCTGGCCGTGGGAATCGAGAAGGGTCTGGCCAACTCGATCCTCGTGAAGGTCAACCAGATCGGCACGCTGTCCGAGAC
CCTGGACGCCGTCGATATGGCCCACCGCAACAGCTACACGGCGGTGATGAGCCACCGCTCGGGCGAGACCGAGGATTCCA
CGATCGCGGACCTCTCAGTCGCCACCAACTGCGGTCAGATCAAGACCGGATCGCTGGCCCGCTCGGACCGTACGGCCAAG
TACAACCAGCTGCTCCGCATCGAGGAGGAGCTGGGCGACCAGGCGATCTACGCCGGCCGCCGGGCGCTGCGGGCGCTCTA
G

Upstream 100 bases:

>100_bases
ATGACCGGCCGACGCCGCGGCAAGTCAACCGGGGAAGTCAAGTTGCCCCTGTCGCGGAAGGTGGCTAAGGCTCGCGCGCC
GCAACACCCGAGGGAGCTCC

Downstream 100 bases:

>100_bases
CGGCGCTCGTCGCCGGGCGGACCCTGATAGTCCGGGCCGTAGACCGCGGCCCGGATGTCTCGGTGCAGGGTCCCGTCGAA
CGGGGCCGTCTGGACGAAGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEIVDITAREILDSRGNPTVEVDVILEDGAMGRAAVPSGASTGAHEAVEKRDGDKARYLGKGVRQAVDAVNGEIYDALS
GSDAEDQRRIDRMLIELDGTPNKSRLGANAILGVSLATAKAAASSSALPLYKYVGGVSARILPVPMMNIINGGAHADNPI
DIQEFMILPTGAETFSEALRMGAEIFHALKKQLKDAGHNTNVGDEGGFAPNIGSAEEALAFIVKAGEGAGYRCGEDFHLG
LDVASTEFFKNGKYVMEGEGKTVDPAGMVEYLAGLVSKFPIVTIEDGCAEDDFDGWKILTERLGGKVQLVGDDLFVTNPE
RLAVGIEKGLANSILVKVNQIGTLSETLDAVDMAHRNSYTAVMSHRSGETEDSTIADLSVATNCGQIKTGSLARSDRTAK
YNQLLRIEEELGDQAIYAGRRALRAL

Sequences:

>Translated_426_residues
MTEIVDITAREILDSRGNPTVEVDVILEDGAMGRAAVPSGASTGAHEAVEKRDGDKARYLGKGVRQAVDAVNGEIYDALS
GSDAEDQRRIDRMLIELDGTPNKSRLGANAILGVSLATAKAAASSSALPLYKYVGGVSARILPVPMMNIINGGAHADNPI
DIQEFMILPTGAETFSEALRMGAEIFHALKKQLKDAGHNTNVGDEGGFAPNIGSAEEALAFIVKAGEGAGYRCGEDFHLG
LDVASTEFFKNGKYVMEGEGKTVDPAGMVEYLAGLVSKFPIVTIEDGCAEDDFDGWKILTERLGGKVQLVGDDLFVTNPE
RLAVGIEKGLANSILVKVNQIGTLSETLDAVDMAHRNSYTAVMSHRSGETEDSTIADLSVATNCGQIKTGSLARSDRTAK
YNQLLRIEEELGDQAIYAGRRALRAL
>Mature_425_residues
TEIVDITAREILDSRGNPTVEVDVILEDGAMGRAAVPSGASTGAHEAVEKRDGDKARYLGKGVRQAVDAVNGEIYDALSG
SDAEDQRRIDRMLIELDGTPNKSRLGANAILGVSLATAKAAASSSALPLYKYVGGVSARILPVPMMNIINGGAHADNPID
IQEFMILPTGAETFSEALRMGAEIFHALKKQLKDAGHNTNVGDEGGFAPNIGSAEEALAFIVKAGEGAGYRCGEDFHLGL
DVASTEFFKNGKYVMEGEGKTVDPAGMVEYLAGLVSKFPIVTIEDGCAEDDFDGWKILTERLGGKVQLVGDDLFVTNPER
LAVGIEKGLANSILVKVNQIGTLSETLDAVDMAHRNSYTAVMSHRSGETEDSTIADLSVATNCGQIKTGSLARSDRTAKY
NQLLRIEEELGDQAIYAGRRALRAL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=52.6558891454965, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=52.3148148148148, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=52.3148148148148, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=50.5827505827506, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=48.1395348837209, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=25.5192878338279, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=25.5192878338279, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=25.5192878338279, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=61.0722610722611, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=51.5011547344111, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=51.5011547344111, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=46.8421052631579, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=50.5773672055427, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=51.2761020881671, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.1154734411085, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.1154734411085, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=50.5800464037123, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=51.6279069767442, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=51.6279069767442, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=51.6279069767442, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=51.6279069767442, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=51.6279069767442, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=51.6279069767442, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PHEZH (B4RBW1)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130587.1
- ProteinModelPortal:   B4RBW1
- GeneID:   6791260
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1747
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45247; Mature: 45116

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIVDITAREILDSRGNPTVEVDVILEDGAMGRAAVPSGASTGAHEAVEKRDGDKARYL
CCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVRQAVDAVNGEIYDALSGSDAEDQRRIDRMLIELDGTPNKSRLGANAILGVSLATAK
HHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHEEEECCCCCHHHCCCCCEEEEHHHHHH
AAASSSALPLYKYVGGVSARILPVPMMNIINGGAHADNPIDIQEFMILPTGAETFSEALR
HHCCCCCCHHHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAEIFHALKKQLKDAGHNTNVGDEGGFAPNIGSAEEALAFIVKAGEGAGYRCGEDFHLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCEEC
LDVASTEFFKNGKYVMEGEGKTVDPAGMVEYLAGLVSKFPIVTIEDGCAEDDFDGWKILT
CCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
ERLGGKVQLVGDDLFVTNPERLAVGIEKGLANSILVKVNQIGTLSETLDAVDMAHRNSYT
HHHCCEEEEEECCEEEECHHHHEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEE
AVMSHRSGETEDSTIADLSVATNCGQIKTGSLARSDRTAKYNQLLRIEEELGDQAIYAGR
EEEECCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
RALRAL
HHHHCC
>Mature Secondary Structure 
TEIVDITAREILDSRGNPTVEVDVILEDGAMGRAAVPSGASTGAHEAVEKRDGDKARYL
CCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVRQAVDAVNGEIYDALSGSDAEDQRRIDRMLIELDGTPNKSRLGANAILGVSLATAK
HHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHEEEECCCCCHHHCCCCCEEEEHHHHHH
AAASSSALPLYKYVGGVSARILPVPMMNIINGGAHADNPIDIQEFMILPTGAETFSEALR
HHCCCCCCHHHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCHHHEEEECCCHHHHHHHHH
MGAEIFHALKKQLKDAGHNTNVGDEGGFAPNIGSAEEALAFIVKAGEGAGYRCGEDFHLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCEEC
LDVASTEFFKNGKYVMEGEGKTVDPAGMVEYLAGLVSKFPIVTIEDGCAEDDFDGWKILT
CCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
ERLGGKVQLVGDDLFVTNPERLAVGIEKGLANSILVKVNQIGTLSETLDAVDMAHRNSYT
HHHCCEEEEEECCEEEECHHHHEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEE
AVMSHRSGETEDSTIADLSVATNCGQIKTGSLARSDRTAKYNQLLRIEEELGDQAIYAGR
EEEECCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
RALRAL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA