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The map label for this gene is pdhB [H]

Identifier: 197105206

GI number: 197105206

Start: 1952424

End: 1953869

Strand: Reverse

Name: pdhB [H]

Synonym: PHZ_c1743

Alternate gene names: 197105206

Gene position: 1953869-1952424 (Counterclockwise)

Preceding gene: 197105207

Following gene: 197105205

Centisome position: 48.89

GC content: 70.12

Gene sequence:

>1446_bases
ATGACCGACATCCTGATGCCGGCCCTGTCGCCCACCATGGAGGAGGGCACCCTCGCCAAGTGGCACGTGAAGCCGGGCGA
CAAGGTGCGCTCCGGCGACGTGATCGCCGAGATCGAAACCGACAAGGCGACCATGGAGGTCGAGGCCGTGGACGACGGCG
TCGTGGCCGAGATCCTGGTGCCGGAAGGCTCGCAGGAGGTGAAGGTCAACACTCCGATCGCCCGCCTGCAGGGCGAGGAC
GGAACCGCGCAGCCCTCCAAGGCCCCCTCCAAGACCGAGGCGGCGCAGACCGAAGCCGTCCAGACCGACACCATCGCGGA
AAAGCCGTTCCGGAAGGAGCCCTCGGGCGACGAGGAGAAGAAGGGCCCCTCCGAGGCCAAGGGCGAGCGCCCCGAGGGCG
AGGGCCCCGCGGCGGTGACGCCCGACCGGCCGCTGGCCGATCCCGAGATCCCCGAGGGCGTCCAGCTCGTGAAGCAGACG
GTCCGCGACGCCCTGCGCGACGCCATGGCCGAGGAGATGCGCCGCGATCCCGACGTCTTCCTGATGGGCGAGGAAGTCGC
CCAGTACCAGGGCGCCTACAAGGTCAGCCGCGGGCTGCTGGACGAGTTCGGCGATCGCCGGGTGATCGACACGCCGATCA
CCGAGCACGGCTTCGCCGGCCTGGGCGTCGGCGCCGGCATGGCCGGGCTGAAGCCGATCGTCGAGTTCATGACCTTCAAC
TTCGCGATGCAGGCGATTGATCAGATTATCAATTCCGCCGCGAAGACCCTTTACATGTCGGGCGGCCAGCTGAAGACCTC
GGTCGTCTTCCGCGGGCCCAACGGCGCGGCCGCCCGGGTGGCGGCGCAGCACAGCCAGGACTACGCGGCCTGGTACGCCC
ACGTGCCGGGCCTGAAGGTGATCGCGCCCTACGACGCCGCCGACGCCAAGGGCCTCCTCAAGGCCGCGATCCGGGATCCG
AACCCCGTGGTCTTCCTCGAGCACGAGATGCTCTACGGCCAGGAGTTCGACGTGCCCGAGGGGATCGACTGGGTCGTGCC
GATCGGCAAGGCCAAGGTCCGCCGGCCGGGCAAGGACGTGACGATCGTCGGCTACTCGCGCATGGTCGGCCTGGCCCTCA
AGGCCGCCGAGGAGCTCGCCGCCGAGGGCATCGAGGCCGAGGTCATCGACCTGCGCACCCTGCGCCCGCTGGACCACGAG
ACGGTCGTCGAGAGCGTGAAGAAGACCAACCGCCTGGTCACCGTCGAGGAAGGCTGGGGCCCGATGGGCGTCGGCGCCGA
GGTGGCGGCCCGCGTGGTGGAGCACGCCTTCGACTGGCTGGACGCCCCGCCTGCGCGGGTGTGCCAGGAGGACGTGCCGC
TGCCCTACGCGGCGAACCTCGAGGCCCTGTCGCTGCCGTCCGTCGAGCGGATCGTGAAGGCCGCCAAGGCGGTCTCGTAC
CGATGA

Upstream 100 bases:

>100_bases
TCGTCGCCGACGCGGCGGAGTTCGCCCGCACCAGCCCCGAACCCGACCCGTCCGAACTCTACACCGACGTCTACACCGAC
GCTTCCCAGGGGGCGGCCCA

Downstream 100 bases:

>100_bases
GCGGCTCGCCCTTCGAGTGGCGCGCCGGCGGATGCCACTGCGGCGGCGTCCGCTTCGAGGTCGCCCTGCCGGACCGGGTC
GAGGCCCAGGCCTGCAACTG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTDILMPALSPTMEEGTLAKWHVKPGDKVRSGDVIAEIETDKATMEVEAVDDGVVAEILVPEGSQEVKVNTPIARLQGED
GTAQPSKAPSKTEAAQTEAVQTDTIAEKPFRKEPSGDEEKKGPSEAKGERPEGEGPAAVTPDRPLADPEIPEGVQLVKQT
VRDALRDAMAEEMRRDPDVFLMGEEVAQYQGAYKVSRGLLDEFGDRRVIDTPITEHGFAGLGVGAGMAGLKPIVEFMTFN
FAMQAIDQIINSAAKTLYMSGGQLKTSVVFRGPNGAAARVAAQHSQDYAAWYAHVPGLKVIAPYDAADAKGLLKAAIRDP
NPVVFLEHEMLYGQEFDVPEGIDWVVPIGKAKVRRPGKDVTIVGYSRMVGLALKAAEELAAEGIEAEVIDLRTLRPLDHE
TVVESVKKTNRLVTVEEGWGPMGVGAEVAARVVEHAFDWLDAPPARVCQEDVPLPYAANLEALSLPSVERIVKAAKAVSY
R

Sequences:

>Translated_481_residues
MTDILMPALSPTMEEGTLAKWHVKPGDKVRSGDVIAEIETDKATMEVEAVDDGVVAEILVPEGSQEVKVNTPIARLQGED
GTAQPSKAPSKTEAAQTEAVQTDTIAEKPFRKEPSGDEEKKGPSEAKGERPEGEGPAAVTPDRPLADPEIPEGVQLVKQT
VRDALRDAMAEEMRRDPDVFLMGEEVAQYQGAYKVSRGLLDEFGDRRVIDTPITEHGFAGLGVGAGMAGLKPIVEFMTFN
FAMQAIDQIINSAAKTLYMSGGQLKTSVVFRGPNGAAARVAAQHSQDYAAWYAHVPGLKVIAPYDAADAKGLLKAAIRDP
NPVVFLEHEMLYGQEFDVPEGIDWVVPIGKAKVRRPGKDVTIVGYSRMVGLALKAAEELAAEGIEAEVIDLRTLRPLDHE
TVVESVKKTNRLVTVEEGWGPMGVGAEVAARVVEHAFDWLDAPPARVCQEDVPLPYAANLEALSLPSVERIVKAAKAVSY
R
>Mature_480_residues
TDILMPALSPTMEEGTLAKWHVKPGDKVRSGDVIAEIETDKATMEVEAVDDGVVAEILVPEGSQEVKVNTPIARLQGEDG
TAQPSKAPSKTEAAQTEAVQTDTIAEKPFRKEPSGDEEKKGPSEAKGERPEGEGPAAVTPDRPLADPEIPEGVQLVKQTV
RDALRDAMAEEMRRDPDVFLMGEEVAQYQGAYKVSRGLLDEFGDRRVIDTPITEHGFAGLGVGAGMAGLKPIVEFMTFNF
AMQAIDQIINSAAKTLYMSGGQLKTSVVFRGPNGAAARVAAQHSQDYAAWYAHVPGLKVIAPYDAADAKGLLKAAIRDPN
PVVFLEHEMLYGQEFDVPEGIDWVVPIGKAKVRRPGKDVTIVGYSRMVGLALKAAEELAAEGIEAEVIDLRTLRPLDHET
VVESVKKTNRLVTVEEGWGPMGVGAEVAARVVEHAFDWLDAPPARVCQEDVPLPYAANLEALSLPSVERIVKAAKAVSYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=61.1111111111111, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=58.0246913580247, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI4557353, Length=338, Percent_Identity=34.9112426035503, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI34101272, Length=338, Percent_Identity=34.9112426035503, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI203098753, Length=79, Percent_Identity=50.6329113924051, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI203098816, Length=79, Percent_Identity=50.6329113924051, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI31711992, Length=71, Percent_Identity=52.112676056338, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI260898739, Length=56, Percent_Identity=57.1428571428571, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=321, Percent_Identity=65.4205607476635, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17506935, Length=335, Percent_Identity=40.2985074626866, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17560088, Length=73, Percent_Identity=50.6849315068493, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539652, Length=304, Percent_Identity=29.2763157894737, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319698, Length=324, Percent_Identity=62.037037037037, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324258, Length=149, Percent_Identity=42.2818791946309, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=112, Percent_Identity=41.0714285714286, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358145, Length=321, Percent_Identity=63.5514018691589, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24650940, Length=321, Percent_Identity=63.5514018691589, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI160714832, Length=319, Percent_Identity=35.7366771159875, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI160714828, Length=319, Percent_Identity=35.7366771159875, Blast_Score=198, Evalue=8e-51,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=68.1318681318681, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=68.1318681318681, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI20129315, Length=64, Percent_Identity=51.5625, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 51829; Mature: 51698

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDILMPALSPTMEEGTLAKWHVKPGDKVRSGDVIAEIETDKATMEVEAVDDGVVAEILV
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEEE
PEGSQEVKVNTPIARLQGEDGTAQPSKAPSKTEAAQTEAVQTDTIAEKPFRKEPSGDEEK
CCCCCCEEECCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHC
KGPSEAKGERPEGEGPAAVTPDRPLADPEIPEGVQLVKQTVRDALRDAMAEEMRRDPDVF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LMGEEVAQYQGAYKVSRGLLDEFGDRRVIDTPITEHGFAGLGVGAGMAGLKPIVEFMTFN
EECHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FAMQAIDQIINSAAKTLYMSGGQLKTSVVFRGPNGAAARVAAQHSQDYAAWYAHVPGLKV
HHHHHHHHHHHHHHHHEEECCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHCCCCCEE
IAPYDAADAKGLLKAAIRDPNPVVFLEHEMLYGQEFDVPEGIDWVVPIGKAKVRRPGKDV
EECCCCCCHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCCCCCEEEECCCHHCCCCCCCE
TIVGYSRMVGLALKAAEELAAEGIEAEVIDLRTLRPLDHETVVESVKKTNRLVTVEEGWG
EEEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHCCCEEEEECCCC
PMGVGAEVAARVVEHAFDWLDAPPARVCQEDVPLPYAANLEALSLPSVERIVKAAKAVSY
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
R
C
>Mature Secondary Structure 
TDILMPALSPTMEEGTLAKWHVKPGDKVRSGDVIAEIETDKATMEVEAVDDGVVAEILV
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEEE
PEGSQEVKVNTPIARLQGEDGTAQPSKAPSKTEAAQTEAVQTDTIAEKPFRKEPSGDEEK
CCCCCCEEECCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHC
KGPSEAKGERPEGEGPAAVTPDRPLADPEIPEGVQLVKQTVRDALRDAMAEEMRRDPDVF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LMGEEVAQYQGAYKVSRGLLDEFGDRRVIDTPITEHGFAGLGVGAGMAGLKPIVEFMTFN
EECHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FAMQAIDQIINSAAKTLYMSGGQLKTSVVFRGPNGAAARVAAQHSQDYAAWYAHVPGLKV
HHHHHHHHHHHHHHHHEEECCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHCCCCCEE
IAPYDAADAKGLLKAAIRDPNPVVFLEHEMLYGQEFDVPEGIDWVVPIGKAKVRRPGKDV
EECCCCCCHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCCCCCEEEECCCHHCCCCCCCE
TIVGYSRMVGLALKAAEELAAEGIEAEVIDLRTLRPLDHETVVESVKKTNRLVTVEEGWG
EEEEHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHCCCEEEEECCCC
PMGVGAEVAARVVEHAFDWLDAPPARVCQEDVPLPYAANLEALSLPSVERIVKAAKAVSY
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]