Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 197105002

GI number: 197105002

Start: 1739491

End: 1740993

Strand: Reverse

Name: purF [H]

Synonym: PHZ_c1539

Alternate gene names: 197105002

Gene position: 1740993-1739491 (Counterclockwise)

Preceding gene: 197105003

Following gene: 197105001

Centisome position: 43.57

GC content: 69.19

Gene sequence:

>1503_bases
ATGACCCTGTCGGCCAAGGCCATGGCGTACGATCGATGGTCCCCGCCGCCGCGGGACCCGGATGACGATACGCCGCGTCT
GGAATGCGGCGTGTTCGGCGTCTTCGACGTCGAAGCGGCCTCGGGGCTCACGGCGCTGGGCCTGCACGCCCTGCAGCACC
GTGGCCAGGAAGCCTGCGGCATCGCCTGCTTCGACGGCCAACGCTTCCACACCGAGCGCCACATGGGGCACGTGGGCGAC
GCCTTCGGCGGCGGCGACCTCAACGAGCGCCTGCCGGGCCAGTCCGCGATCGGGCACACTCGCTATTCGACCGCCGGCGG
CAGCTTCATCCGCAACGTCCAGCCGATGTTCGCCGACCTCGAGGCGGGCGGGATCGCCCTCGCCCACAACGGCAACCTCA
CCAATTTCCTGACGCTGCGCGAGCAGCTGGTCGCCCAGGGCGCCATCTTCCAGTCCACCTCGGACTCCGAGGTCATCCTC
CACCTGATCGCCCGGTCGCGTAAGGCCAAGTTCGTCGAGCGGTTCATCGACGCCCTGCAGCAGATCGAGGGCGGCTACGC
CCTGGTGGCGCTGACCAACAAGAAGCTGATCGGCGTGCGCGACCCGCTCGGCATCCGGCCGCTCGTCCTCGGCGATCTCG
ACGGCAAGGCGGTGCTCGCCTCCGAGACCTGCGCCCTGGACATGGTCGGCGCCCGCTTCGTGCGCGACGTCGAGCACGGC
GAAATGGTGGTCATCGACCAGGACGGGGTGCAGAGCCTTCGGCCCTTCCCGGCCATGCAGGCGCGCCCCTGCATCTTCGA
ATACGTCTACTTCGCCCGCCCGGACTCGGTCGTGAACGGCCGCTCGGTCTACGAGGTCCGCAAGCGGATGGGCCGGCGCC
TGGCGCAGGAGACCCCCGCCGACGTCGACGTCGTGGTGCCGGTGCCGGACTCCGGCGTCCCCGCCGCCCTGGGCTTCGCC
CAGGAGGCGGGCCTTCCCTTCGAGATGGGGATCATCCGGAATCACTATGTGGGCCGGACCTTCATCGAGCCGACCCAGGG
CCAGCGCGAGCTGGGCGTGCGGATGAAGCTGTCGCCCAACCGCTCGGTCCTGGCGGGCAAGCGCGTGCTGCTGGTGGACG
ATTCCATCGTGCGCGGCACCAATTCGGTGCGCGTGGTGCGCATGGTCCGCGAGGCGGGCGCCAAGGAGGTGCATCTGCGC
TCGGCCTCGCCGCCGATCCAGTGGCCCGACTACTACGGCATCGACATGCCCGACCGCGAGAAGCTGCTGGCGGCCAACCA
CTCGGTCGAAGAGATCGCCAAGATCCTGAACGTGGACTCCATGGGCTACCTCTCGGTGGACGGGCTCTACTGGGCGATGG
AAGCCGCGCGCGATCCGGCCAATCCCCAGTTCACCGACCACTATTTCACCGGCGACTACCCCACGCGCCTGCTGGACCGT
GAGATCGCCGAGGGCCGCAACCAGGCCGTGGAGCGCCAGCTCTCGTTCCTGGTCGACGCGTGA

Upstream 100 bases:

>100_bases
CCGCTTTCGACCGGGCGGTGCGAAACGGGTCAGGTGACAGACGCGTGACGGACGGCTACGACGCGCCGCGAAACGGCGAG
ACGGATCTGGAGAGGTCTCG

Downstream 100 bases:

>100_bases
GCGCGCCCCGGGCGGGCCTGCTCTCCCGGATCAAGATCAAGCCCGACTGGTACCTGATCCTGATCGTGCTGATGGGCGCG
GCCGCGACGATCGCGCCTGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTLSAKAMAYDRWSPPPRDPDDDTPRLECGVFGVFDVEAASGLTALGLHALQHRGQEACGIACFDGQRFHTERHMGHVGD
AFGGGDLNERLPGQSAIGHTRYSTAGGSFIRNVQPMFADLEAGGIALAHNGNLTNFLTLREQLVAQGAIFQSTSDSEVIL
HLIARSRKAKFVERFIDALQQIEGGYALVALTNKKLIGVRDPLGIRPLVLGDLDGKAVLASETCALDMVGARFVRDVEHG
EMVVIDQDGVQSLRPFPAMQARPCIFEYVYFARPDSVVNGRSVYEVRKRMGRRLAQETPADVDVVVPVPDSGVPAALGFA
QEAGLPFEMGIIRNHYVGRTFIEPTQGQRELGVRMKLSPNRSVLAGKRVLLVDDSIVRGTNSVRVVRMVREAGAKEVHLR
SASPPIQWPDYYGIDMPDREKLLAANHSVEEIAKILNVDSMGYLSVDGLYWAMEAARDPANPQFTDHYFTGDYPTRLLDR
EIAEGRNQAVERQLSFLVDA

Sequences:

>Translated_500_residues
MTLSAKAMAYDRWSPPPRDPDDDTPRLECGVFGVFDVEAASGLTALGLHALQHRGQEACGIACFDGQRFHTERHMGHVGD
AFGGGDLNERLPGQSAIGHTRYSTAGGSFIRNVQPMFADLEAGGIALAHNGNLTNFLTLREQLVAQGAIFQSTSDSEVIL
HLIARSRKAKFVERFIDALQQIEGGYALVALTNKKLIGVRDPLGIRPLVLGDLDGKAVLASETCALDMVGARFVRDVEHG
EMVVIDQDGVQSLRPFPAMQARPCIFEYVYFARPDSVVNGRSVYEVRKRMGRRLAQETPADVDVVVPVPDSGVPAALGFA
QEAGLPFEMGIIRNHYVGRTFIEPTQGQRELGVRMKLSPNRSVLAGKRVLLVDDSIVRGTNSVRVVRMVREAGAKEVHLR
SASPPIQWPDYYGIDMPDREKLLAANHSVEEIAKILNVDSMGYLSVDGLYWAMEAARDPANPQFTDHYFTGDYPTRLLDR
EIAEGRNQAVERQLSFLVDA
>Mature_499_residues
TLSAKAMAYDRWSPPPRDPDDDTPRLECGVFGVFDVEAASGLTALGLHALQHRGQEACGIACFDGQRFHTERHMGHVGDA
FGGGDLNERLPGQSAIGHTRYSTAGGSFIRNVQPMFADLEAGGIALAHNGNLTNFLTLREQLVAQGAIFQSTSDSEVILH
LIARSRKAKFVERFIDALQQIEGGYALVALTNKKLIGVRDPLGIRPLVLGDLDGKAVLASETCALDMVGARFVRDVEHGE
MVVIDQDGVQSLRPFPAMQARPCIFEYVYFARPDSVVNGRSVYEVRKRMGRRLAQETPADVDVVVPVPDSGVPAALGFAQ
EAGLPFEMGIIRNHYVGRTFIEPTQGQRELGVRMKLSPNRSVLAGKRVLLVDDSIVRGTNSVRVVRMVREAGAKEVHLRS
ASPPIQWPDYYGIDMPDREKLLAANHSVEEIAKILNVDSMGYLSVDGLYWAMEAARDPANPQFTDHYFTGDYPTRLLDRE
IAEGRNQAVERQLSFLVDA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=461, Percent_Identity=40.1301518438178, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=40.6315789473684, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1790167, Length=229, Percent_Identity=30.1310043668122, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=37.9958246346555, Blast_Score=300, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6323958, Length=485, Percent_Identity=38.5567010309278, Blast_Score=279, Evalue=9e-76,
Organism=Drosophila melanogaster, GI28573187, Length=493, Percent_Identity=40.5679513184584, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24659598, Length=501, Percent_Identity=38.5229540918164, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=39.7408207343413, Blast_Score=318, Evalue=5e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54923; Mature: 54792

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSAKAMAYDRWSPPPRDPDDDTPRLECGVFGVFDVEAASGLTALGLHALQHRGQEACG
CCCCCCHHEECCCCCCCCCCCCCCCCEEEEEEEEEECHHHCCHHHHHHHHHHHCCCCCCE
IACFDGQRFHTERHMGHVGDAFGGGDLNERLPGQSAIGHTRYSTAGGSFIRNVQPMFADL
EEEECCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCEECCCCCHHHHCCCHHHHCC
EAGGIALAHNGNLTNFLTLREQLVAQGAIFQSTSDSEVILHLIARSRKAKFVERFIDALQ
CCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QIEGGYALVALTNKKLIGVRDPLGIRPLVLGDLDGKAVLASETCALDMVGARFVRDVEHG
HHCCCEEEEEEECCEEEECCCCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCC
EMVVIDQDGVQSLRPFPAMQARPCIFEYVYFARPDSVVNGRSVYEVRKRMGRRLAQETPA
CEEEEECCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
DVDVVVPVPDSGVPAALGFAQEAGLPFEMGIIRNHYVGRTFIEPTQGQRELGVRMKLSPN
CEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHCCCCCCEECCCCCCCHHCCEEEEECCC
RSVLAGKRVLLVDDSIVRGTNSVRVVRMVREAGAKEVHLRSASPPIQWPDYYGIDMPDRE
CCEECCCEEEEECCHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHH
KLLAANHSVEEIAKILNVDSMGYLSVDGLYWAMEAARDPANPQFTDHYFTGDYPTRLLDR
HHHHCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHH
EIAEGRNQAVERQLSFLVDA
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLSAKAMAYDRWSPPPRDPDDDTPRLECGVFGVFDVEAASGLTALGLHALQHRGQEACG
CCCCCHHEECCCCCCCCCCCCCCCCEEEEEEEEEECHHHCCHHHHHHHHHHHCCCCCCE
IACFDGQRFHTERHMGHVGDAFGGGDLNERLPGQSAIGHTRYSTAGGSFIRNVQPMFADL
EEEECCCCHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCEECCCCCHHHHCCCHHHHCC
EAGGIALAHNGNLTNFLTLREQLVAQGAIFQSTSDSEVILHLIARSRKAKFVERFIDALQ
CCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QIEGGYALVALTNKKLIGVRDPLGIRPLVLGDLDGKAVLASETCALDMVGARFVRDVEHG
HHCCCEEEEEEECCEEEECCCCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCC
EMVVIDQDGVQSLRPFPAMQARPCIFEYVYFARPDSVVNGRSVYEVRKRMGRRLAQETPA
CEEEEECCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
DVDVVVPVPDSGVPAALGFAQEAGLPFEMGIIRNHYVGRTFIEPTQGQRELGVRMKLSPN
CEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHCCCCCCEECCCCCCCHHCCEEEEECCC
RSVLAGKRVLLVDDSIVRGTNSVRVVRMVREAGAKEVHLRSASPPIQWPDYYGIDMPDRE
CCEECCCEEEEECCHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHH
KLLAANHSVEEIAKILNVDSMGYLSVDGLYWAMEAARDPANPQFTDHYFTGDYPTRLLDR
HHHHCCCCHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCEECCCCHHHHHHH
EIAEGRNQAVERQLSFLVDA
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA