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The map label for this gene is trpI [H]

Identifier: 197104879

GI number: 197104879

Start: 1600960

End: 1601853

Strand: Reverse

Name: trpI [H]

Synonym: PHZ_c1413

Alternate gene names: 197104879

Gene position: 1601853-1600960 (Counterclockwise)

Preceding gene: 197104892

Following gene: 197104876

Centisome position: 40.08

GC content: 73.38

Gene sequence:

>894_bases
ATGTTCGAAAAACTCAAAAGCGGCCCGCCGGCATCCCTGAACGCGCTTCGGGCCTTCGAGGCTGTGGCGCGCACCGGCAA
GGTGACGCTGGCGGCCGAGGAACTGCACGTGACGCCGTCGGCCGTCAGCCGCCAGGTGAAGGCGCTGGAGGCGAGCCTGG
GCGTGCGGCTCATCTCGGGGCCGAAGCATCGCCTGGCCCTGACGCCCGCGGGCCGGGATCTGGCCCCCTCTCTCACCGAG
GCGTTCGACCGGATCGACGCGGCGGTGCGGCGGACGCGTCAGGGCGGGGAGGACCTGCATGTGGCGGTCAACGCCAGCCT
GTCGGTGAAATGGCTGATCCCCCGCCTGGCCGGCTTCCAGGCCCGGCACCCTGACATCCGCCTGCACCTGTCGGAGCTGG
CGCCGCACGCCACCTCCCAGCGCGGCGCCCACGCCGTGGTAAGGATCGTGCCCCTCGCCCGCCTCTCGGATCCCAACTGC
ACGCCCTTCATCCGCAACGCCCTGGGCGCGGTGATGTCGCCGGAGTGCGCCGCCCGGCACGGGAACGACGTGCTGGCCGC
GCCGCGGCTGGCCGCCGTCACCCACCCCGAGGCGTGGCCGATCTTCGCCGAGCTCGCCGGGCTGGAGCTGCCGCTGGTCA
ACGAGCGGCCCTTCGCCCACGTCCACTTCGCAATGGACGCGGCGCTGGCCGGCCTGGGCGCGGCGGTGCTGTCGTGGCCG
CTGTGCGTGGACGAGGTGCGGAGGGGTCGGCTCGTCGCGCCGGCGGGCTTCCACCGCTCGGCCAGCGCTTTCGCGATGCT
GACGACGCCGGGGGCGGAAAGCCGGGCCCTTTCGAAGTTCCGCGACTGGCTGGTGGCGGAAGGCGCTAGGACGCCGGAGC
CGCCCGCCCCTTGA

Upstream 100 bases:

>100_bases
ACCACCAGGGCCTTGGAGGGATCGGATCGAAGCGAAGCCGTCATGGCCCGCGTTCATGCCGCCCTGGGCTGATGTCGAAA
AACGACTTATTGGCTCCAGT

Downstream 100 bases:

>100_bases
ACCCCGTCGCGACCAGGAAGACCTCGGAGCTGTCCTGCCGGCTGGCCTTCGGCTTGATGTTGCGGACGTCCTTGAAGCGG
GTCTTCAGCCCCGCGATGAT

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MFEKLKSGPPASLNALRAFEAVARTGKVTLAAEELHVTPSAVSRQVKALEASLGVRLISGPKHRLALTPAGRDLAPSLTE
AFDRIDAAVRRTRQGGEDLHVAVNASLSVKWLIPRLAGFQARHPDIRLHLSELAPHATSQRGAHAVVRIVPLARLSDPNC
TPFIRNALGAVMSPECAARHGNDVLAAPRLAAVTHPEAWPIFAELAGLELPLVNERPFAHVHFAMDAALAGLGAAVLSWP
LCVDEVRRGRLVAPAGFHRSASAFAMLTTPGAESRALSKFRDWLVAEGARTPEPPAP

Sequences:

>Translated_297_residues
MFEKLKSGPPASLNALRAFEAVARTGKVTLAAEELHVTPSAVSRQVKALEASLGVRLISGPKHRLALTPAGRDLAPSLTE
AFDRIDAAVRRTRQGGEDLHVAVNASLSVKWLIPRLAGFQARHPDIRLHLSELAPHATSQRGAHAVVRIVPLARLSDPNC
TPFIRNALGAVMSPECAARHGNDVLAAPRLAAVTHPEAWPIFAELAGLELPLVNERPFAHVHFAMDAALAGLGAAVLSWP
LCVDEVRRGRLVAPAGFHRSASAFAMLTTPGAESRALSKFRDWLVAEGARTPEPPAP
>Mature_297_residues
MFEKLKSGPPASLNALRAFEAVARTGKVTLAAEELHVTPSAVSRQVKALEASLGVRLISGPKHRLALTPAGRDLAPSLTE
AFDRIDAAVRRTRQGGEDLHVAVNASLSVKWLIPRLAGFQARHPDIRLHLSELAPHATSQRGAHAVVRIVPLARLSDPNC
TPFIRNALGAVMSPECAARHGNDVLAAPRLAAVTHPEAWPIFAELAGLELPLVNERPFAHVHFAMDAALAGLGAAVLSWP
LCVDEVRRGRLVAPAGFHRSASAFAMLTTPGAESRALSKFRDWLVAEGARTPEPPAP

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=30.8196721311475, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=29.2253521126761, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=25.3472222222222, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31668; Mature: 31668

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKLKSGPPASLNALRAFEAVARTGKVTLAAEELHVTPSAVSRQVKALEASLGVRLISG
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCEEEECC
PKHRLALTPAGRDLAPSLTEAFDRIDAAVRRTRQGGEDLHVAVNASLSVKWLIPRLAGFQ
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEHHHHHCCCC
ARHPDIRLHLSELAPHATSQRGAHAVVRIVPLARLSDPNCTPFIRNALGAVMSPECAARH
CCCCCCEEEHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHCCCHHHHHC
GNDVLAAPRLAAVTHPEAWPIFAELAGLELPLVNERPFAHVHFAMDAALAGLGAAVLSWP
CCCEECCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
LCVDEVRRGRLVAPAGFHRSASAFAMLTTPGAESRALSKFRDWLVAEGARTPEPPAP
HHHHHHHCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFEKLKSGPPASLNALRAFEAVARTGKVTLAAEELHVTPSAVSRQVKALEASLGVRLISG
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCEEEECC
PKHRLALTPAGRDLAPSLTEAFDRIDAAVRRTRQGGEDLHVAVNASLSVKWLIPRLAGFQ
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEHHHHHCCCC
ARHPDIRLHLSELAPHATSQRGAHAVVRIVPLARLSDPNCTPFIRNALGAVMSPECAARH
CCCCCCEEEHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCCCHHHHHHHHHHCCCHHHHHC
GNDVLAAPRLAAVTHPEAWPIFAELAGLELPLVNERPFAHVHFAMDAALAGLGAAVLSWP
CCCEECCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
LCVDEVRRGRLVAPAGFHRSASAFAMLTTPGAESRALSKFRDWLVAEGARTPEPPAP
HHHHHHHCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]