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The map label for this gene is amtB [H]

Identifier: 197104826

GI number: 197104826

Start: 1542116

End: 1543279

Strand: Reverse

Name: amtB [H]

Synonym: PHZ_c1360

Alternate gene names: 197104826

Gene position: 1543279-1542116 (Counterclockwise)

Preceding gene: 197104827

Following gene: 197104822

Centisome position: 38.62

GC content: 69.07

Gene sequence:

>1164_bases
GTGCTGGCCATGACCGTTCCAGGCCTGGCGCTGTTCTACGGCGGCATGGTCCGGCGCAAGAACGTGATCGCCACGGTCGC
CCAGTGCTTCGCGATCACCGCGGTGATCAGCGTCGTCTGGATGGTGCTGGGCTATTCCCTCGCCTTCTCCGAGAACCCGT
CGGCGGATCTCAACGCCTATGTCGGCGGGTTCGGCGCGGCCATGCTGAACGGCATCGGCATCGACGACGTGTCGGGCAGC
ATTCCGCAGTACCTGTTCGTCGTCTTCCAGATGACCTTCGCGATCATCACGCCCGCCCTGATCGTCGGCGCGTTCGCCGA
GCGGATGAAGTTCTCGGCCATGCTGCTGTTCATGATCCTGTGGTCGCTGGTGGTCTACGCGCCGGTCTGCCACTGGGTGT
GGGGCGGCGGCTTCATGTCCGAGCACGGCGTCCTCGACTTCGCCGGCGGCACGGTGGTGCACATCAACGCCGGCGTCGCG
GGCCTCGTCTGCGCGCTCGTCCTCGGCAAGCGCAAGGGCTACGGGACCGACAACATGGCGCCGCACAACCTCGTGCTGAC
CATGATCGGCGCCAGCCTCCTGTGGGTCGGCTGGTTCGGCTTCAACGCCGGCTCGGCCGTGGCCGCCAACGGCCTTGCGG
CGGTGGCCATGCTGAACACCCAGGTCGCCACGGCGGCCGCGGCGGTGGCCTGGATGATCATCGAGTGGGTCGAGCGTAAG
AAGCCCGGCATGCTGGGCCTGGCCTCGGGGGCGGTGGCGGGCCTCGTGGCCATCACGCCGGCCGCGGGCTTCGTGAACCC
GCAGGGCGCGCTGATCATCGGCCTCGTCGGCGGCGCCGCGGCCTACGCCGGCGCGGTCTGGATCAAGCGGGCCCTCAAGT
ACGACGACAGCCTCGATGTCTTCGGCGTCCACGGGGTCGCGGGCATCGCCGGCGCCCTGCTGACGGGCCTGCTGGCCGAC
GCCGCCATCGGCGGCGACGCGGCGGCGAACGCCAGCCTCGGCGCGCAGTTCTTCGGCATCATCGGCACCGTCGTCTGGTG
CGCCGTGGCCACCTTCGTGATCCTGATGATCCTCAAGTTCACGCTGGGCCTGCGCGTCAGCGAGGAGGCGGAGGTCGACG
GTCTCGACATCTCGCAGCACGGCGAAGCACTGCACGAGACCTGA

Upstream 100 bases:

>100_bases
GCCCCCGGCCGCGGAGCCGACCGAGGCCGCCGCGGCAGCCGAGGAGACCCCCACCGCCGACAGCGGCGACACCGCCTGGC
TGCTGACCTCCACCGCGCTG

Downstream 100 bases:

>100_bases
TCCGCTCCGGGGGAGAGGCGTCGCCTCGCGGCGGCGTCTTCGGGGTGGCGCGGGGGTCCTTCGGGGCCCCCGCTTCCGTT
TGGGCGGTCAGGCCGCGTCC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLAMTVPGLALFYGGMVRRKNVIATVAQCFAITAVISVVWMVLGYSLAFSENPSADLNAYVGGFGAAMLNGIGIDDVSGS
IPQYLFVVFQMTFAIITPALIVGAFAERMKFSAMLLFMILWSLVVYAPVCHWVWGGGFMSEHGVLDFAGGTVVHINAGVA
GLVCALVLGKRKGYGTDNMAPHNLVLTMIGASLLWVGWFGFNAGSAVAANGLAAVAMLNTQVATAAAAVAWMIIEWVERK
KPGMLGLASGAVAGLVAITPAAGFVNPQGALIIGLVGGAAAYAGAVWIKRALKYDDSLDVFGVHGVAGIAGALLTGLLAD
AAIGGDAAANASLGAQFFGIIGTVVWCAVATFVILMILKFTLGLRVSEEAEVDGLDISQHGEALHET

Sequences:

>Translated_387_residues
MLAMTVPGLALFYGGMVRRKNVIATVAQCFAITAVISVVWMVLGYSLAFSENPSADLNAYVGGFGAAMLNGIGIDDVSGS
IPQYLFVVFQMTFAIITPALIVGAFAERMKFSAMLLFMILWSLVVYAPVCHWVWGGGFMSEHGVLDFAGGTVVHINAGVA
GLVCALVLGKRKGYGTDNMAPHNLVLTMIGASLLWVGWFGFNAGSAVAANGLAAVAMLNTQVATAAAAVAWMIIEWVERK
KPGMLGLASGAVAGLVAITPAAGFVNPQGALIIGLVGGAAAYAGAVWIKRALKYDDSLDVFGVHGVAGIAGALLTGLLAD
AAIGGDAAANASLGAQFFGIIGTVVWCAVATFVILMILKFTLGLRVSEEAEVDGLDISQHGEALHET
>Mature_387_residues
MLAMTVPGLALFYGGMVRRKNVIATVAQCFAITAVISVVWMVLGYSLAFSENPSADLNAYVGGFGAAMLNGIGIDDVSGS
IPQYLFVVFQMTFAIITPALIVGAFAERMKFSAMLLFMILWSLVVYAPVCHWVWGGGFMSEHGVLDFAGGTVVHINAGVA
GLVCALVLGKRKGYGTDNMAPHNLVLTMIGASLLWVGWFGFNAGSAVAANGLAAVAMLNTQVATAAAAVAWMIIEWVERK
KPGMLGLASGAVAGLVAITPAAGFVNPQGALIIGLVGGAAAYAGAVWIKRALKYDDSLDVFGVHGVAGIAGALLTGLLAD
AAIGGDAAANASLGAQFFGIIGTVVWCAVATFVILMILKFTLGLRVSEEAEVDGLDISQHGEALHET

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=387, Percent_Identity=49.8708010335917, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17550182, Length=412, Percent_Identity=28.6407766990291, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=411, Percent_Identity=28.4671532846715, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=350, Percent_Identity=29.1428571428571, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=411, Percent_Identity=28.4671532846715, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17563228, Length=173, Percent_Identity=30.635838150289, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324187, Length=405, Percent_Identity=36.7901234567901, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6325396, Length=401, Percent_Identity=36.1596009975062, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321559, Length=398, Percent_Identity=34.6733668341709, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI161078314, Length=392, Percent_Identity=27.8061224489796, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 39903; Mature: 39903

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAMTVPGLALFYGGMVRRKNVIATVAQCFAITAVISVVWMVLGYSLAFSENPSADLNAY
CCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHH
VGGFGAAMLNGIGIDDVSGSIPQYLFVVFQMTFAIITPALIVGAFAERMKFSAMLLFMIL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSLVVYAPVCHWVWGGGFMSEHGVLDFAGGTVVHINAGVAGLVCALVLGKRKGYGTDNMA
HHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
PHNLVLTMIGASLLWVGWFGFNAGSAVAANGLAAVAMLNTQVATAAAAVAWMIIEWVERK
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPGMLGLASGAVAGLVAITPAAGFVNPQGALIIGLVGGAAAYAGAVWIKRALKYDDSLDV
CCCCEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCE
FGVHGVAGIAGALLTGLLADAAIGGDAAANASLGAQFFGIIGTVVWCAVATFVILMILKF
EECCHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGLRVSEEAEVDGLDISQHGEALHET
HHCCCCCCCCCCCCCCCHHHCHHHCCC
>Mature Secondary Structure
MLAMTVPGLALFYGGMVRRKNVIATVAQCFAITAVISVVWMVLGYSLAFSENPSADLNAY
CCEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCHHHH
VGGFGAAMLNGIGIDDVSGSIPQYLFVVFQMTFAIITPALIVGAFAERMKFSAMLLFMIL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSLVVYAPVCHWVWGGGFMSEHGVLDFAGGTVVHINAGVAGLVCALVLGKRKGYGTDNMA
HHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
PHNLVLTMIGASLLWVGWFGFNAGSAVAANGLAAVAMLNTQVATAAAAVAWMIIEWVERK
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPGMLGLASGAVAGLVAITPAAGFVNPQGALIIGLVGGAAAYAGAVWIKRALKYDDSLDV
CCCCEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCE
FGVHGVAGIAGALLTGLLADAAIGGDAAANASLGAQFFGIIGTVVWCAVATFVILMILKF
EECCHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGLRVSEEAEVDGLDISQHGEALHET
HHCCCCCCCCCCCCCCCHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]