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The map label for this gene is proP [H]

Identifier: 197104806

GI number: 197104806

Start: 1518403

End: 1519863

Strand: Direct

Name: proP [H]

Synonym: PHZ_c1340

Alternate gene names: 197104806

Gene position: 1518403-1519863 (Clockwise)

Preceding gene: 197104805

Following gene: 197104807

Centisome position: 38.0

GC content: 71.66

Gene sequence:

>1461_bases
ATGCGGGGGCGGGCGGCTGCGCTCGGCGGCCTGAGGCGTCGACGCCGGATCGAAGTGGGGGCTGACATGACGATGGAACA
GGACGGCCGGGGCCCTGCGGCGCCGGCCGAGGCGGCCGCCGTCCGCCGGGCGGTGGCGGCTTCGGCGGTGGGCAACGCCA
CCGAGTGGTTCGACTACGGGATCTACGCCTACGGGGTGACCTACATCTCGGCGGCGCTGTTTCCGGGGGAGGCCGCGCAG
GCGACGCTGTTCGCGTTGGCGACGTTCGCGATCTCGTTCCTGGTGCGGCCGCTGGGGGGCTTCTTCTGGGGCCCGCTGGG
CGACCGCCTGGGGCGCAAGAGCGTGCTGGCCTTCACCATCGTGCTGATGGCCGCCGCCACGTTCCTGGTGGGCCTCCTGC
CGACCCACGAGGCGGTGGGCTTCTGGGCGCCGCTGATGCTGGTGATCCTGCGGATGGTGCAGGGCTTCTCCACCGGCGGG
GAGTACGGCGGTGCCGCGACGTTCATGGCGGAGTACGCGCCCGACGACCGGCGCGGCTTCTTCGGCAGCTTCCTGGAGGT
CGGCACCCTGGCGGGCTTCTCGTTCGGGGCCCTGCTGATGCTGGGCTTCTCGCTGCTGCTGGGGAACGAGGCCATGCACG
AGTGGGGCTGGCGCCTGCCGTTCCTGGTGGCCGCGCCGCTGGGGCTCGTCGGTCTCTACCTGCGGGCCAAGATCGAGGAC
ACGCCGGTGTTCCGAGAGGCCGAGGCGCTGGAGGCCGAATCCGCCAGCCGCCAGCCCGGCCTGAAGACGCTGTTCACCGG
CTACTGGCGCCCGCTGCTGGTGATGAGCGGCCTCGTCATCGCGCTGAACGTCGTGAACTACACCCTGCTCAGCTACATGC
CGACCTATCTCGAGCGACGCCTGGGCCTTTCGACCCAGCAGGCGCTGCTGGTCCCGATCATCGGCATGCTGTGCATGATG
GCGCTGCTGCCCTTCGCGGGCGCGGCCTCAGACCGGGTGGGGCGCAAGCCGCTGTGGGCGGCCTCGCTGGTCGGGCTCTT
CGTCCTGGCGATCCCGCTCTACCACCTGATGGGCGCGGGCCTTTCCGGGGCGATCGTCGGTTTCGCCGTACTGGGGCTGC
TCTACGCGCCGCAACTGGCGACGATCTCGGCGACCTTCCCGGCCATGTTCCCGACGCGCGTGCGGTTCGCGGGCTTCGCG
GTCGCCTACAACGTGGCCACCTCGCTGTTCGGCGGCACCGCGCCGGCCGCCAATTCCTGGCTGATCGAGCGGACGGGCGA
CGCCGCCGTCCCCGCCTACTACATGATGGCCGCCTGCGTGGTGGGCCTGATCGCCCTGGCCTTCACGGCCGAGACCGCCG
GCCAGTCGATCCGCGGCGACGCGGGCGCGCCTGCATCCTCTCGCCGAGGCGGCGCCGTCCGGGGCGGGCAGGGGACAGTG
TCGGGAAACTTGGAGGCCTGA

Upstream 100 bases:

>100_bases
TCCTGGGCATCCAGCAGCTCACCCGCCGGGTGGATCTGCAGTTCGTGGTCCGGGAGCGGGACTACGCCGCGAGCGTCCAG
GCCCTGCACGCGCGCCTGGT

Downstream 100 bases:

>100_bases
CCGGGAATCGAACCCGGGTACGCGGATTTGCAGTCCGCTGCGTCACCACTCCGCCATCAGGCCGTCTCGAAGGCCCTTGG
GCTCTCCGGAGGGCGCGAGG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRGRAAALGGLRRRRRIEVGADMTMEQDGRGPAAPAEAAAVRRAVAASAVGNATEWFDYGIYAYGVTYISAALFPGEAAQ
ATLFALATFAISFLVRPLGGFFWGPLGDRLGRKSVLAFTIVLMAAATFLVGLLPTHEAVGFWAPLMLVILRMVQGFSTGG
EYGGAATFMAEYAPDDRRGFFGSFLEVGTLAGFSFGALLMLGFSLLLGNEAMHEWGWRLPFLVAAPLGLVGLYLRAKIED
TPVFREAEALEAESASRQPGLKTLFTGYWRPLLVMSGLVIALNVVNYTLLSYMPTYLERRLGLSTQQALLVPIIGMLCMM
ALLPFAGAASDRVGRKPLWAASLVGLFVLAIPLYHLMGAGLSGAIVGFAVLGLLYAPQLATISATFPAMFPTRVRFAGFA
VAYNVATSLFGGTAPAANSWLIERTGDAAVPAYYMMAACVVGLIALAFTAETAGQSIRGDAGAPASSRRGGAVRGGQGTV
SGNLEA

Sequences:

>Translated_486_residues
MRGRAAALGGLRRRRRIEVGADMTMEQDGRGPAAPAEAAAVRRAVAASAVGNATEWFDYGIYAYGVTYISAALFPGEAAQ
ATLFALATFAISFLVRPLGGFFWGPLGDRLGRKSVLAFTIVLMAAATFLVGLLPTHEAVGFWAPLMLVILRMVQGFSTGG
EYGGAATFMAEYAPDDRRGFFGSFLEVGTLAGFSFGALLMLGFSLLLGNEAMHEWGWRLPFLVAAPLGLVGLYLRAKIED
TPVFREAEALEAESASRQPGLKTLFTGYWRPLLVMSGLVIALNVVNYTLLSYMPTYLERRLGLSTQQALLVPIIGMLCMM
ALLPFAGAASDRVGRKPLWAASLVGLFVLAIPLYHLMGAGLSGAIVGFAVLGLLYAPQLATISATFPAMFPTRVRFAGFA
VAYNVATSLFGGTAPAANSWLIERTGDAAVPAYYMMAACVVGLIALAFTAETAGQSIRGDAGAPASSRRGGAVRGGQGTV
SGNLEA
>Mature_486_residues
MRGRAAALGGLRRRRRIEVGADMTMEQDGRGPAAPAEAAAVRRAVAASAVGNATEWFDYGIYAYGVTYISAALFPGEAAQ
ATLFALATFAISFLVRPLGGFFWGPLGDRLGRKSVLAFTIVLMAAATFLVGLLPTHEAVGFWAPLMLVILRMVQGFSTGG
EYGGAATFMAEYAPDDRRGFFGSFLEVGTLAGFSFGALLMLGFSLLLGNEAMHEWGWRLPFLVAAPLGLVGLYLRAKIED
TPVFREAEALEAESASRQPGLKTLFTGYWRPLLVMSGLVIALNVVNYTLLSYMPTYLERRLGLSTQQALLVPIIGMLCMM
ALLPFAGAASDRVGRKPLWAASLVGLFVLAIPLYHLMGAGLSGAIVGFAVLGLLYAPQLATISATFPAMFPTRVRFAGFA
VAYNVATSLFGGTAPAANSWLIERTGDAAVPAYYMMAACVVGLIALAFTAETAGQSIRGDAGAPASSRRGGAVRGGQGTV
SGNLEA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=464, Percent_Identity=41.8103448275862, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1788292, Length=412, Percent_Identity=31.7961165048544, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=404, Percent_Identity=32.1782178217822, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=34.8946135831382, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87082404, Length=375, Percent_Identity=23.4666666666667, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082231, Length=283, Percent_Identity=27.208480565371, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 51218; Mature: 51218

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCC
>Mature Secondary Structure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CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]