Click here to switch to the map view.

The map label for this gene is mscM [H]

Identifier: 197104799

GI number: 197104799

Start: 1511810

End: 1513036

Strand: Direct

Name: mscM [H]

Synonym: PHZ_c1333

Alternate gene names: 197104799

Gene position: 1511810-1513036 (Clockwise)

Preceding gene: 197104798

Following gene: 197104801

Centisome position: 37.83

GC content: 69.03

Gene sequence:

>1227_bases
ATGACCCTCGCAGACCTGACCGCCTTCGCCGCCCCCTATCCCTGGCTGGAGACCCTCTGGGCGCTCGCCCTGCTGGTCGC
CGCCGCCGCCGTCGCCAACCTGCTGGTCAAGCCGCTGATCGTGCGGGTGCTGGCCCGGGTCTTCGACGGCCTCTCGCACC
GGGTCAGCCACGAGGACGCCCTGCCGGTCGCCCGCCGGCTCGCCAACATCCTGCCGGCGGTGGTGATCGCGCAGGGGATC
GTGCTGGTGCCGCACCTGCCGGTCGGCGTGGTCACGGTGACCCGCAATGTCTGCAGCGCCTTCGTCATCCTGATGGTCGT
GCTGGCCCTCAGCGCGGCGCTCAACCTGCTGAACGACCTCTACCAGCGGCGGCCGACGGCCATGAGCCGGCCGATCAAGG
GCTACCTGCAGGTGGTCAAGATCCTGCTGTTCGCCGCCGCCGCGGTGCTGATGGCGGCGGCGCTGATGGAACGCTCGCCG
CTGCTGCTCCTCTCGGGCCTGGGCGCCATGGCCGCGGTGCTGATGCTGGTGTTCAAGGACACCATCCTGTCGCTGGTGGC
CTCGGTGCAGATCGCCTCCAACGACATGGTCCGCCTCGGCGACTGGGTGGAGATGCCTCAGCTGAACGCCGACGGGGACG
TGATCGACATCGCCCTGCACACGGTGAAGATCCAGAACTGGGACAAGACCATCACCACGATCCCGACGCAGCGGCTGATC
ACCGACTCGTTCAAGAACTGGCGCGGCATGCAGGAGTCGGGCGGGCGGCGGATCAAGCGCGCCCTGATGCTGGACCAGAC
GAGCGTCCGCTTCCTCGCCGACGAGGATCGGGAGCGCCTGCGGCGGATGTCGCTGATCGCCGACTACCTCGATCGCAAGC
AGGCCGAGCTGGACGCCTGGAACCAGCGGCTGCTGGCGGACGGGCGCGATCCCGTGAACACCCGCCAGCTCACCAACCTC
GGCACCTTCCGCGCCTATGTGCAGGCCTACCTCGAAGCCCATCCCACGATCGCCAGGAACATGACCCTGCTGGTCCGGCA
GCTTCAGCCGACCCCCGAGGGCCTGCCGATCGAGATCTACTGCTTCACGGCCACGACCGCCTGGGCCGAGTACGAGGGCG
TGCAGTCGGACATCTTCGACCACCTCTACGCGGTCCTGCCCGAGTTCGGCCTGCGGGTGTTCCAGGCCCCGACCGGCCTC
GACCTCCAGCGGCTGGCGGCCGCCTAG

Upstream 100 bases:

>100_bases
CCCAAAGACGCCGGAACCGTTCGCCGCGCGCGCCAGGCGCGCCGGTTCTCCCAGGCGCACCCCCCGGCGGCCACTCGGGC
CGCGCCGACATGCCGGAGCC

Downstream 100 bases:

>100_bases
GTCTCGGGCGGCGCCGAGCGCTGCAGCGAGCCGATGTCGAGGATCGGCGAGGCGTCGATGGTGTCGGCGTCCAGCAGCAC
GGCGACCCGCTCCAGGGCCG

Product: small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTLADLTAFAAPYPWLETLWALALLVAAAAVANLLVKPLIVRVLARVFDGLSHRVSHEDALPVARRLANILPAVVIAQGI
VLVPHLPVGVVTVTRNVCSAFVILMVVLALSAALNLLNDLYQRRPTAMSRPIKGYLQVVKILLFAAAAVLMAAALMERSP
LLLLSGLGAMAAVLMLVFKDTILSLVASVQIASNDMVRLGDWVEMPQLNADGDVIDIALHTVKIQNWDKTITTIPTQRLI
TDSFKNWRGMQESGGRRIKRALMLDQTSVRFLADEDRERLRRMSLIADYLDRKQAELDAWNQRLLADGRDPVNTRQLTNL
GTFRAYVQAYLEAHPTIARNMTLLVRQLQPTPEGLPIEIYCFTATTAWAEYEGVQSDIFDHLYAVLPEFGLRVFQAPTGL
DLQRLAAA

Sequences:

>Translated_408_residues
MTLADLTAFAAPYPWLETLWALALLVAAAAVANLLVKPLIVRVLARVFDGLSHRVSHEDALPVARRLANILPAVVIAQGI
VLVPHLPVGVVTVTRNVCSAFVILMVVLALSAALNLLNDLYQRRPTAMSRPIKGYLQVVKILLFAAAAVLMAAALMERSP
LLLLSGLGAMAAVLMLVFKDTILSLVASVQIASNDMVRLGDWVEMPQLNADGDVIDIALHTVKIQNWDKTITTIPTQRLI
TDSFKNWRGMQESGGRRIKRALMLDQTSVRFLADEDRERLRRMSLIADYLDRKQAELDAWNQRLLADGRDPVNTRQLTNL
GTFRAYVQAYLEAHPTIARNMTLLVRQLQPTPEGLPIEIYCFTATTAWAEYEGVQSDIFDHLYAVLPEFGLRVFQAPTGL
DLQRLAAA
>Mature_407_residues
TLADLTAFAAPYPWLETLWALALLVAAAAVANLLVKPLIVRVLARVFDGLSHRVSHEDALPVARRLANILPAVVIAQGIV
LVPHLPVGVVTVTRNVCSAFVILMVVLALSAALNLLNDLYQRRPTAMSRPIKGYLQVVKILLFAAAAVLMAAALMERSPL
LLLSGLGAMAAVLMLVFKDTILSLVASVQIASNDMVRLGDWVEMPQLNADGDVIDIALHTVKIQNWDKTITTIPTQRLIT
DSFKNWRGMQESGGRRIKRALMLDQTSVRFLADEDRERLRRMSLIADYLDRKQAELDAWNQRLLADGRDPVNTRQLTNLG
TFRAYVQAYLEAHPTIARNMTLLVRQLQPTPEGLPIEIYCFTATTAWAEYEGVQSDIFDHLYAVLPEFGLRVFQAPTGLD
LQRLAAA

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=312, Percent_Identity=51.6025641025641, Blast_Score=343, Evalue=8e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 45147; Mature: 45015

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLADLTAFAAPYPWLETLWALALLVAAAAVANLLVKPLIVRVLARVFDGLSHRVSHEDA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LPVARRLANILPAVVIAQGIVLVPHLPVGVVTVTRNVCSAFVILMVVLALSAALNLLNDL
HHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQRRPTAMSRPIKGYLQVVKILLFAAAAVLMAAALMERSPLLLLSGLGAMAAVLMLVFKD
HHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TILSLVASVQIASNDMVRLGDWVEMPQLNADGDVIDIALHTVKIQNWDKTITTIPTQRLI
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHCCHHHHH
TDSFKNWRGMQESGGRRIKRALMLDQTSVRFLADEDRERLRRMSLIADYLDRKQAELDAW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQRLLADGRDPVNTRQLTNLGTFRAYVQAYLEAHPTIARNMTLLVRQLQPTPEGLPIEIY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEE
CFTATTAWAEYEGVQSDIFDHLYAVLPEFGLRVFQAPTGLDLQRLAAA
EEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
>Mature Secondary Structure 
TLADLTAFAAPYPWLETLWALALLVAAAAVANLLVKPLIVRVLARVFDGLSHRVSHEDA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LPVARRLANILPAVVIAQGIVLVPHLPVGVVTVTRNVCSAFVILMVVLALSAALNLLNDL
HHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQRRPTAMSRPIKGYLQVVKILLFAAAAVLMAAALMERSPLLLLSGLGAMAAVLMLVFKD
HHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
TILSLVASVQIASNDMVRLGDWVEMPQLNADGDVIDIALHTVKIQNWDKTITTIPTQRLI
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHCCHHHHH
TDSFKNWRGMQESGGRRIKRALMLDQTSVRFLADEDRERLRRMSLIADYLDRKQAELDAW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQRLLADGRDPVNTRQLTNLGTFRAYVQAYLEAHPTIARNMTLLVRQLQPTPEGLPIEIY
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEE
CFTATTAWAEYEGVQSDIFDHLYAVLPEFGLRVFQAPTGLDLQRLAAA
EEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]