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The map label for this gene is potF [H]

Identifier: 197104670

GI number: 197104670

Start: 1388288

End: 1389388

Strand: Reverse

Name: potF [H]

Synonym: PHZ_c1204

Alternate gene names: 197104670

Gene position: 1389388-1388288 (Counterclockwise)

Preceding gene: 197104672

Following gene: 197104669

Centisome position: 34.77

GC content: 69.48

Gene sequence:

>1101_bases
ATGGCGCACTGGGCGCACTGGGCGAAGGCGGTCGGGCTGGCTCTGGCCCTGATGCTGGCGGCCTGCGGCGGAAAGCCCGC
CGAGGAGCTGCGGATCTACAACTGGTCGGACTACATCGACCCCGCCCTGCTCGACGCCTTCACCGAGGAGACCGGCGTCC
GCGTCGTCTACGACACCTTCGATTCCAACGAGGTGCTCGAGACCAAGGTGCTGCAGGGAAACACCGGCTACGACCTGGTG
GTCCCGTCCAACCACAACCTGCCCCGCTACATCGCCGCCGGCGCGGTCCAGCCGCTGGACAAGGCCAAGCTGCCGGGGCT
GTCGAACCTCTCGCCGCCGCTGATGGAGCGGCTGGCCGCGTTCGATCCGGGCGCGAAGTACGCCGTGCCCTACATGCAGG
GCACGATCGGCATCGGCTACAACGCCGCCGAGGTGGCCAGGCGCCTGCCGGGCGTCGCCATCGACAGCTGGGCGGTGGTG
CTCGAGCCGGCCAACCTCGCCCGGCTGCAGGACTGCGGCGTCTACTTCCTCGACGCCTCCGAGGACATGTACGCCGTCGC
GCTCAACTACCTGGGCAAGGACCCGAATTCGACCGCGCCGGCCGACTACGCGGCCGCCACCGACCTGCTGCTGAAGGCCC
GGCCCTACGTGCGCAAGTTCCACTCGTCCGAATACATTGAGGCCCTGGCGAACGGCGACGCCTGCGTGGCGGTCGGCTAT
TCGGGCGACGTGCTGCAGGCCAAGGCGCGCGCCCAGGAGGCGAAGACCGGCGTCGCCCTGGGCTATGTGGTCCCGAAGGA
GGGCAGCCAGGTCTGGTTCGACGTCTTCGCCATCCCGAAGGACGCGCCGAACCCCGATGCGGCGCACCGCTTCATCGCCT
ACATGCTCCGCCCCGAGGTGATCGCGCGGGCTTCCGACTACACCCAGTACGCCAACGCCAACGCCGCGGCGACGGCGCTG
GTGGACCCGGCGGTGCGGAACGATCCCAACGTCTATCCGGGCCCCGAGCTGATGGACCGGCTCTTCGTCACCACCACCAA
GGATCAGGCCCTGCTGCGCGAGGTGAACCGCCAGTGGACGCGGGTCCTGACCGGCCGGTGA

Upstream 100 bases:

>100_bases
CGCGGTCCAGAGGGCGGGCATCGGCGGGGCGCTCGGCTTTTCGGGCTTAACGGGTCGGAACGAGCCCCTATAACCTGCGC
CGCAAGCTTTGGGGGATGGC

Downstream 100 bases:

>100_bases
GCACCGCCGCCGACATCGTCCGCTTCGAGGGCGTGAGCCGGCGCTTCGCGGGCCACGCCGCCGTGGACGGGGTCGACCTC
GCCATCCGCGAAGGCGAGTT

Product: putrescine ABC transporter, periplasmic putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAHWAHWAKAVGLALALMLAACGGKPAEELRIYNWSDYIDPALLDAFTEETGVRVVYDTFDSNEVLETKVLQGNTGYDLV
VPSNHNLPRYIAAGAVQPLDKAKLPGLSNLSPPLMERLAAFDPGAKYAVPYMQGTIGIGYNAAEVARRLPGVAIDSWAVV
LEPANLARLQDCGVYFLDASEDMYAVALNYLGKDPNSTAPADYAAATDLLLKARPYVRKFHSSEYIEALANGDACVAVGY
SGDVLQAKARAQEAKTGVALGYVVPKEGSQVWFDVFAIPKDAPNPDAAHRFIAYMLRPEVIARASDYTQYANANAAATAL
VDPAVRNDPNVYPGPELMDRLFVTTTKDQALLREVNRQWTRVLTGR

Sequences:

>Translated_366_residues
MAHWAHWAKAVGLALALMLAACGGKPAEELRIYNWSDYIDPALLDAFTEETGVRVVYDTFDSNEVLETKVLQGNTGYDLV
VPSNHNLPRYIAAGAVQPLDKAKLPGLSNLSPPLMERLAAFDPGAKYAVPYMQGTIGIGYNAAEVARRLPGVAIDSWAVV
LEPANLARLQDCGVYFLDASEDMYAVALNYLGKDPNSTAPADYAAATDLLLKARPYVRKFHSSEYIEALANGDACVAVGY
SGDVLQAKARAQEAKTGVALGYVVPKEGSQVWFDVFAIPKDAPNPDAAHRFIAYMLRPEVIARASDYTQYANANAAATAL
VDPAVRNDPNVYPGPELMDRLFVTTTKDQALLREVNRQWTRVLTGR
>Mature_365_residues
AHWAHWAKAVGLALALMLAACGGKPAEELRIYNWSDYIDPALLDAFTEETGVRVVYDTFDSNEVLETKVLQGNTGYDLVV
PSNHNLPRYIAAGAVQPLDKAKLPGLSNLSPPLMERLAAFDPGAKYAVPYMQGTIGIGYNAAEVARRLPGVAIDSWAVVL
EPANLARLQDCGVYFLDASEDMYAVALNYLGKDPNSTAPADYAAATDLLLKARPYVRKFHSSEYIEALANGDACVAVGYS
GDVLQAKARAQEAKTGVALGYVVPKEGSQVWFDVFAIPKDAPNPDAAHRFIAYMLRPEVIARASDYTQYANANAAATALV
DPAVRNDPNVYPGPELMDRLFVTTTKDQALLREVNRQWTRVLTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=358, Percent_Identity=53.3519553072626, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=32.7102803738318, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1787711, Length=293, Percent_Identity=26.962457337884, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39769; Mature: 39638

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHWAHWAKAVGLALALMLAACGGKPAEELRIYNWSDYIDPALLDAFTEETGVRVVYDTF
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHCCHHHHHHHHHCCCCEEEEECC
DSNEVLETKVLQGNTGYDLVVPSNHNLPRYIAAGAVQPLDKAKLPGLSNLSPPLMERLAA
CCCCEEHEEEEECCCCEEEEECCCCCCCHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHH
FDPGAKYAVPYMQGTIGIGYNAAEVARRLPGVAIDSWAVVLEPANLARLQDCGVYFLDAS
CCCCCCEECCEECCCEECCCCHHHHHHHCCCCEECCEEEEECCCCHHHHHHCCEEEEECC
EDMYAVALNYLGKDPNSTAPADYAAATDLLLKARPYVRKFHSSEYIEALANGDACVAVGY
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCEEEEECC
SGDVLQAKARAQEAKTGVALGYVVPKEGSQVWFDVFAIPKDAPNPDAAHRFIAYMLRPEV
CCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IARASDYTQYANANAAATALVDPAVRNDPNVYPGPELMDRLFVTTTKDQALLREVNRQWT
HHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCCCHHHHHHHHEECCHHHHHHHHHHHHHH
RVLTGR
HHHCCC
>Mature Secondary Structure 
AHWAHWAKAVGLALALMLAACGGKPAEELRIYNWSDYIDPALLDAFTEETGVRVVYDTF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHCCHHHHHHHHHCCCCEEEEECC
DSNEVLETKVLQGNTGYDLVVPSNHNLPRYIAAGAVQPLDKAKLPGLSNLSPPLMERLAA
CCCCEEHEEEEECCCCEEEEECCCCCCCHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHH
FDPGAKYAVPYMQGTIGIGYNAAEVARRLPGVAIDSWAVVLEPANLARLQDCGVYFLDAS
CCCCCCEECCEECCCEECCCCHHHHHHHCCCCEECCEEEEECCCCHHHHHHCCEEEEECC
EDMYAVALNYLGKDPNSTAPADYAAATDLLLKARPYVRKFHSSEYIEALANGDACVAVGY
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCCCEEEEECC
SGDVLQAKARAQEAKTGVALGYVVPKEGSQVWFDVFAIPKDAPNPDAAHRFIAYMLRPEV
CCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IARASDYTQYANANAAATALVDPAVRNDPNVYPGPELMDRLFVTTTKDQALLREVNRQWT
HHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCCCHHHHHHHHEECCHHHHHHHHHHHHHH
RVLTGR
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]