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The map label for this gene is potB [H]

Identifier: 197104668

GI number: 197104668

Start: 1386430

End: 1387314

Strand: Reverse

Name: potB [H]

Synonym: PHZ_c1202

Alternate gene names: 197104668

Gene position: 1387314-1386430 (Counterclockwise)

Preceding gene: 197104669

Following gene: 197104667

Centisome position: 34.72

GC content: 72.54

Gene sequence:

>885_bases
ATGAGGCGCGACCGGTCGGGCCTGGCGGCCCTGCTGCCCTATGCGTGGCTGCTGGTCTTCTTCGGCTTTCCCGTCCTGCT
GGTCGCCAGGCTCTCGCTGTCCGACACGGCGCTCGCGATGCCGCCCTACGCGCCGCGGATCGACTGGAGCGCCGGTGTCG
CCGGGCTGCGGGCGTTCCTGGCGGGGCTGGACCTCGAGACCTACGCCCGCCTCGCCGCCGACCGGCTCTACCTGGACGCC
TACGCCTCCAGTCTGCGGATCAGCGCCACGGCCACCGTCCTCCTGCTGCTGGTCGGCTACCCGATGGCCTGGGGGATCGT
CCGCTGCCGGCCGCGCCTGCGCAGCGCCCTCGTCCTGGCCGTGATCCTGCCGTTCTGGACCAGCTTCCTGATCCGCATCT
ACGCCTGGATCGCCATCCTCAAGCCCGCCGGCCTGCTCAACGCCGTCCTCGGCGCCGCCGGCCTGCCGCCGGTCGACCTG
CTGAACACCGAAGCCGCGGTGATCCTCGGGCTCGTCTACGCCTACCTGCCGTTCATGGTGCTGCCGCTCTACGCGGTGCT
GGAACGCCGGGACGAGCGGCTGGTGGAGGCGGCGCGGGATCTGGGGGCCGGCCCGCTGCGGGCGTTCTGGACCGTCACCT
TCCCGCTGTCGCTGCCCGGGGTGGCCGCCGGCGCCCTGCTCTGCTTCATCCCCATGGCGGGCGAGTTCGTGGTCCCCGAC
CTGCTGGGCGGCTCCGAGACCCTGATGCTGGGCCGGGTGATCTGGACCGAGTTCTTCGCCAACCGCGACTGGCCGGCGGC
CTCGGCGGTGGCGATTGCGCTGCTCGCCACCCTGCTCGTCCCCATCCTGCTGTTCGAGCGCCAGCCCCGCCGGGTGCTGG
GATGA

Upstream 100 bases:

>100_bases
TCCTCGCCAGCCGGGCCAACACCGCGGGGGAGCCGCCCGCGCCGTCCGGCGCGCCGGTCTGGCTGTCGTGGCCTGCCGAG
GCGGCCTGGGACCTGCCCGC

Downstream 100 bases:

>100_bases
GGCGCGGACCCTCGGCCTTCGGCGTCGCGGCCATCGGGCTCGGCCTGCTGTTCCTCTACGCGCCCATCGCCATCCTGGTG
CTCTACTCCTTCAACGACGG

Product: spermidine/putrescine ABC transporter, permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRRDRSGLAALLPYAWLLVFFGFPVLLVARLSLSDTALAMPPYAPRIDWSAGVAGLRAFLAGLDLETYARLAADRLYLDA
YASSLRISATATVLLLLVGYPMAWGIVRCRPRLRSALVLAVILPFWTSFLIRIYAWIAILKPAGLLNAVLGAAGLPPVDL
LNTEAAVILGLVYAYLPFMVLPLYAVLERRDERLVEAARDLGAGPLRAFWTVTFPLSLPGVAAGALLCFIPMAGEFVVPD
LLGGSETLMLGRVIWTEFFANRDWPAASAVAIALLATLLVPILLFERQPRRVLG

Sequences:

>Translated_294_residues
MRRDRSGLAALLPYAWLLVFFGFPVLLVARLSLSDTALAMPPYAPRIDWSAGVAGLRAFLAGLDLETYARLAADRLYLDA
YASSLRISATATVLLLLVGYPMAWGIVRCRPRLRSALVLAVILPFWTSFLIRIYAWIAILKPAGLLNAVLGAAGLPPVDL
LNTEAAVILGLVYAYLPFMVLPLYAVLERRDERLVEAARDLGAGPLRAFWTVTFPLSLPGVAAGALLCFIPMAGEFVVPD
LLGGSETLMLGRVIWTEFFANRDWPAASAVAIALLATLLVPILLFERQPRRVLG
>Mature_294_residues
MRRDRSGLAALLPYAWLLVFFGFPVLLVARLSLSDTALAMPPYAPRIDWSAGVAGLRAFLAGLDLETYARLAADRLYLDA
YASSLRISATATVLLLLVGYPMAWGIVRCRPRLRSALVLAVILPFWTSFLIRIYAWIAILKPAGLLNAVLGAAGLPPVDL
LNTEAAVILGLVYAYLPFMVLPLYAVLERRDERLVEAARDLGAGPLRAFWTVTFPLSLPGVAAGALLCFIPMAGEFVVPD
LLGGSETLMLGRVIWTEFFANRDWPAASAVAIALLATLLVPILLFERQPRRVLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=296, Percent_Identity=48.9864864864865, Blast_Score=267, Evalue=7e-73,
Organism=Escherichia coli, GI1787369, Length=228, Percent_Identity=42.1052631578947, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1787713, Length=220, Percent_Identity=31.8181818181818, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31990; Mature: 31990

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRDRSGLAALLPYAWLLVFFGFPVLLVARLSLSDTALAMPPYAPRIDWSAGVAGLRAFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHH
AGLDLETYARLAADRLYLDAYASSLRISATATVLLLLVGYPMAWGIVRCRPRLRSALVLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VILPFWTSFLIRIYAWIAILKPAGLLNAVLGAAGLPPVDLLNTEAAVILGLVYAYLPFMV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
LPLYAVLERRDERLVEAARDLGAGPLRAFWTVTFPLSLPGVAAGALLCFIPMAGEFVVPD
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHCCCCEECHH
LLGGSETLMLGRVIWTEFFANRDWPAASAVAIALLATLLVPILLFERQPRRVLG
HCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MRRDRSGLAALLPYAWLLVFFGFPVLLVARLSLSDTALAMPPYAPRIDWSAGVAGLRAFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHH
AGLDLETYARLAADRLYLDAYASSLRISATATVLLLLVGYPMAWGIVRCRPRLRSALVLA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VILPFWTSFLIRIYAWIAILKPAGLLNAVLGAAGLPPVDLLNTEAAVILGLVYAYLPFMV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
LPLYAVLERRDERLVEAARDLGAGPLRAFWTVTFPLSLPGVAAGALLCFIPMAGEFVVPD
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHCCCCEECHH
LLGGSETLMLGRVIWTEFFANRDWPAASAVAIALLATLLVPILLFERQPRRVLG
HCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]