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The map label for this gene is purU [H]

Identifier: 197104547

GI number: 197104547

Start: 1221877

End: 1222719

Strand: Reverse

Name: purU [H]

Synonym: PHZ_c1081

Alternate gene names: 197104547

Gene position: 1222719-1221877 (Counterclockwise)

Preceding gene: 197104548

Following gene: 197104541

Centisome position: 30.6

GC content: 70.58

Gene sequence:

>843_bases
ATGATCCTGACCCTCTCCTGCCCCGACCAGCCCGGCATCGTTGCCCGCGTGTCCGCCCTGCTCTTCGAGCGCGGCGCCAA
CATCCTCGACGCCCAGCAGTTCGACGACGAGGAGACCGGCCGGTTCTTCATGCGCGTGGTGTTCGATCCGGGCGACGCCG
GCGCCGAGGCCTGGCGCGCCGCCTTCGCGCCCCTCGCCGAGCAGTACGCCATGACCTGGACCCTGCGCGGCCGCAAGGAG
CGCCGCCGGGTGATGATCCTCGCCTCGCAGCAGGACCACTGCCTCTCCGATCTGATCTGGCGCTGGCGGCAGGGCGAGCT
GCAGATGGATCTGACCGCCGTGGTCTCCAACCATCCGGCCTCCACCTTCCCCCACACCGACCTGCAGGGGATCGCCTTCC
ACCACCTGCCGATCACGCCCGAGACCAAGCCCCAGCAGGAGGCCCGCCTCTGGTCGCTGATCGAGGAGACCCGGACCGAA
CTCGTGGTCCTCGCCCGCTACATGCAGGTGCTCTCCGACGACCTCGCCGGCAAGCTCGAGGGCCGCTGCATCAACATCCA
CCACTCGTTCCTGCCGGGCTTCAAGGGCGCCCGCCCCTACCACCAGGCCCACGCCCGCGGGGTGAAGGTGATCGGCGCCA
CGGCCCACTACGTCACCGGCGACCTCGACGAGGGCCCGATCATCGAGCAGGACGTCGAGCGCATCAGCCACCGCGACACG
CCGGCCGCCCTGATCCGCAAGGGCCGCGACATCGAGCGCCGCGTGCTGGCCCGCGCCGTCCGCTGGCGCCTGGAGGACCG
CGTGCTCCTCAACGGCCGCAAGACGGTGGTCTTCACGGACTAG

Upstream 100 bases:

>100_bases
CATGGCGCCACCTTACGGCCGCCGCGCCCGGCCGCAACCATCCACCGCGGGCCACCGCGGGCTTGACCGTGCCGCCCGGG
CTTGCGACGTGCGCCGGGCG

Downstream 100 bases:

>100_bases
GCCCGAGCGCGTCAGGCGAAAGTGGAGGCCGGTTTCGCCGTCCGACGCGCGACCACTAAGAACCCCAGGCCTCCCACAGC
AGCTGCGCGCCCACCAGGAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGDAGAEAWRAAFAPLAEQYAMTWTLRGRKE
RRRVMILASQQDHCLSDLIWRWRQGELQMDLTAVVSNHPASTFPHTDLQGIAFHHLPITPETKPQQEARLWSLIEETRTE
LVVLARYMQVLSDDLAGKLEGRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTGDLDEGPIIEQDVERISHRDT
PAALIRKGRDIERRVLARAVRWRLEDRVLLNGRKTVVFTD

Sequences:

>Translated_280_residues
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGDAGAEAWRAAFAPLAEQYAMTWTLRGRKE
RRRVMILASQQDHCLSDLIWRWRQGELQMDLTAVVSNHPASTFPHTDLQGIAFHHLPITPETKPQQEARLWSLIEETRTE
LVVLARYMQVLSDDLAGKLEGRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTGDLDEGPIIEQDVERISHRDT
PAALIRKGRDIERRVLARAVRWRLEDRVLLNGRKTVVFTD
>Mature_280_residues
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGDAGAEAWRAAFAPLAEQYAMTWTLRGRKE
RRRVMILASQQDHCLSDLIWRWRQGELQMDLTAVVSNHPASTFPHTDLQGIAFHHLPITPETKPQQEARLWSLIEETRTE
LVVLARYMQVLSDDLAGKLEGRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTGDLDEGPIIEQDVERISHRDT
PAALIRKGRDIERRVLARAVRWRLEDRVLLNGRKTVVFTD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=203, Percent_Identity=30.0492610837438, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI209869995, Length=203, Percent_Identity=30.0492610837438, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI209869993, Length=203, Percent_Identity=30.0492610837438, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1787483, Length=283, Percent_Identity=39.2226148409894, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=25.6544502617801, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=164, Percent_Identity=34.1463414634146, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31831; Mature: 31831

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGDAGAEAWRA
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCCCHHHHHH
AFAPLAEQYAMTWTLRGRKERRRVMILASQQDHCLSDLIWRWRQGELQMDLTAVVSNHPA
HHHHHHHHHEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHCCCCC
STFPHTDLQGIAFHHLPITPETKPQQEARLWSLIEETRTELVVLARYMQVLSDDLAGKLE
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTGDLDEGPIIEQDVERISHRDT
CEEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECEEHEEECCCCCCCCCHHHHHHHHCCCC
PAALIRKGRDIERRVLARAVRWRLEDRVLLNGRKTVVFTD
HHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEC
>Mature Secondary Structure
MILTLSCPDQPGIVARVSALLFERGANILDAQQFDDEETGRFFMRVVFDPGDAGAEAWRA
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEECCCCCCHHHHHH
AFAPLAEQYAMTWTLRGRKERRRVMILASQQDHCLSDLIWRWRQGELQMDLTAVVSNHPA
HHHHHHHHHEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHCCCCC
STFPHTDLQGIAFHHLPITPETKPQQEARLWSLIEETRTELVVLARYMQVLSDDLAGKLE
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRCINIHHSFLPGFKGARPYHQAHARGVKVIGATAHYVTGDLDEGPIIEQDVERISHRDT
CEEEEEEHHHCCCCCCCCCHHHHHHCCEEEEECEEHEEECCCCCCCCCHHHHHHHHCCCC
PAALIRKGRDIERRVLARAVRWRLEDRVLLNGRKTVVFTD
HHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]