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The map label for this gene is dctA [H]

Identifier: 197104506

GI number: 197104506

Start: 1165014

End: 1166348

Strand: Direct

Name: dctA [H]

Synonym: PHZ_c1040

Alternate gene names: 197104506

Gene position: 1165014-1166348 (Clockwise)

Preceding gene: 197104505

Following gene: 197104510

Centisome position: 29.15

GC content: 70.41

Gene sequence:

>1335_bases
TTGGCGACACTACCGGCGGCCGGCGCGGCGGCGCCCATCAGGCGCACGCCGATCTGGAACCAGCTCTATGTGCAGGTGCT
GGTGGCGATCGCCGTCGGCGGGGCGATCGGCCACTTCTGGCCGGAGACCGGCGAGGCGCTGAAGCCGCTGGGCGACGCCT
TCATCAAGCTGGTGAAGATGATCATCGCCCCGGTGATCTTCCTGACCGTCACCACGGGCATCGCCGGCATGCGCGACATC
GGCAAGGTGGGCGGGGTGGCGGCCAAGGCGTTCGGCTACTTCATCGTGGTGTCCAGCTTCGCCCTGGTGGTGGGGCTGGT
GGTGGCCAATGTGGTCCAGCCGGGCGCCGGGATGAACGTCGACCCGGCCACCCTCGACGCCGGGGCGGTGCAGGACTACG
CGGCCAAGTCGCACGAGATGACCATCACCGGCTTCCTGATGGACATCATCCCCGCGACGGTCGCCGGGGCCTTCGTCAGC
GGCAACATCCTGCAGGTGCTGTTCTTCTCGATCCCGTTCGGCCTGGCGCTGGCGATGATCGGCGAGCGGGGCGAGCCGGC
GCTGGCGGTGCTGAACGCCGTGGCCGAGGCGTTCTTCCGGCTGGTGGCGATCTTCATGCGGGCGGCGCCCATCGGGGCGC
TGGGGGCGTTCGCCTTCACGGTGGGCCGGTACGGGCTGGAGAGCATCGTCAACCTGGCGGCCCTGGTGGGCACGTTCTAC
GCCACCTCGCTGATCTTCGTGCTGGTGGTGCTGGGGGCGATCGCCCGGCTGCACGGCTTCTCGATCCTGCGGCTGATCCG
CTACCTGAAGGAGGAGCTGCTGCTGGTGCTGGGGACCTCGTCCTCGGAGGCGGCGCTGCCCAGCCTGCTCGAGAAGATGG
AGCGGGCCGGGGCGCCCAAGACGGTGGTCGGGCTGGTGGTGCCCACCGGCTACAGCTTCAACCTCGACGGCACGAACATC
TACATGACCCTGGCGGCCCTGTTCATCGCCCAGGCGGTGGGGATCGACCTGCCGCTGGGCGAACAGCTGCTGCTGCTGGC
GGTGGCGATGCTGAGCTCGAAGGGGGCGGCGGGCGTCACGGGGGCCGGGTTCATCACCCTGGCCGCGACGCTGTCGGTCG
TGCCCAGCGTGCCGGTGGCGGGGATGGCGCTGATCCTGGGCGTCGACCGGTTCATGAGCGAGTGCCGGGCGCTGACCAAC
TTCGTCGGCAATGCGGTGGCGACCCTGGTGGTCAGCCGCTGGGAAGGGCAGCTCGACCGGCAGGCGCTGGAGGACGCGCT
GTCGGGCGCGCCGCAGCCGATCGCGGCCCCGCCGGTGGAGGCCGACGCGGACTAG

Upstream 100 bases:

>100_bases
GGCGGGCGGCGCACGGATCTCGACTGGTTCTGAGGCCGCGGCTGGTTCTGGGGCCGGCGGACCCTTATAGCCTCGCCGCG
AGGAAGTTGGGGGACGACAC

Downstream 100 bases:

>100_bases
GAGCGGCCTTCGCGGGTTCAGCTCGCGCGGGAGAGCGGCGTGGCGCGGGCGGCCGCGGCGCGGGTGACCGAGGCCATCAG
CCCCGAGACCAGGATGGGCT

Product: C4-dicarboxylate transport protein

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MATLPAAGAAAPIRRTPIWNQLYVQVLVAIAVGGAIGHFWPETGEALKPLGDAFIKLVKMIIAPVIFLTVTTGIAGMRDI
GKVGGVAAKAFGYFIVVSSFALVVGLVVANVVQPGAGMNVDPATLDAGAVQDYAAKSHEMTITGFLMDIIPATVAGAFVS
GNILQVLFFSIPFGLALAMIGERGEPALAVLNAVAEAFFRLVAIFMRAAPIGALGAFAFTVGRYGLESIVNLAALVGTFY
ATSLIFVLVVLGAIARLHGFSILRLIRYLKEELLLVLGTSSSEAALPSLLEKMERAGAPKTVVGLVVPTGYSFNLDGTNI
YMTLAALFIAQAVGIDLPLGEQLLLLAVAMLSSKGAAGVTGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTN
FVGNAVATLVVSRWEGQLDRQALEDALSGAPQPIAAPPVEADAD

Sequences:

>Translated_444_residues
MATLPAAGAAAPIRRTPIWNQLYVQVLVAIAVGGAIGHFWPETGEALKPLGDAFIKLVKMIIAPVIFLTVTTGIAGMRDI
GKVGGVAAKAFGYFIVVSSFALVVGLVVANVVQPGAGMNVDPATLDAGAVQDYAAKSHEMTITGFLMDIIPATVAGAFVS
GNILQVLFFSIPFGLALAMIGERGEPALAVLNAVAEAFFRLVAIFMRAAPIGALGAFAFTVGRYGLESIVNLAALVGTFY
ATSLIFVLVVLGAIARLHGFSILRLIRYLKEELLLVLGTSSSEAALPSLLEKMERAGAPKTVVGLVVPTGYSFNLDGTNI
YMTLAALFIAQAVGIDLPLGEQLLLLAVAMLSSKGAAGVTGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTN
FVGNAVATLVVSRWEGQLDRQALEDALSGAPQPIAAPPVEADAD
>Mature_443_residues
ATLPAAGAAAPIRRTPIWNQLYVQVLVAIAVGGAIGHFWPETGEALKPLGDAFIKLVKMIIAPVIFLTVTTGIAGMRDIG
KVGGVAAKAFGYFIVVSSFALVVGLVVANVVQPGAGMNVDPATLDAGAVQDYAAKSHEMTITGFLMDIIPATVAGAFVSG
NILQVLFFSIPFGLALAMIGERGEPALAVLNAVAEAFFRLVAIFMRAAPIGALGAFAFTVGRYGLESIVNLAALVGTFYA
TSLIFVLVVLGAIARLHGFSILRLIRYLKEELLLVLGTSSSEAALPSLLEKMERAGAPKTVVGLVVPTGYSFNLDGTNIY
MTLAALFIAQAVGIDLPLGEQLLLLAVAMLSSKGAAGVTGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNF
VGNAVATLVVSRWEGQLDRQALEDALSGAPQPIAAPPVEADAD

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane. This transport system plays an important role in the energy supply of rhizobium-legume symbionts [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=430, Percent_Identity=25.8139534883721, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI21314632, Length=426, Percent_Identity=25.5868544600939, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI194239697, Length=468, Percent_Identity=23.7179487179487, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI66773030, Length=425, Percent_Identity=26.5882352941176, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI4827012, Length=282, Percent_Identity=29.4326241134752, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI5032093, Length=409, Percent_Identity=28.361858190709, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI40254478, Length=429, Percent_Identity=25.1748251748252, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI223468566, Length=330, Percent_Identity=30.3030303030303, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI223468564, Length=300, Percent_Identity=30.6666666666667, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI262359914, Length=346, Percent_Identity=24.8554913294798, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI301601644, Length=166, Percent_Identity=31.3253012048193, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789947, Length=419, Percent_Identity=58.9498806682578, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1790514, Length=409, Percent_Identity=41.0757946210269, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1788024, Length=395, Percent_Identity=24.5569620253165, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17537407, Length=406, Percent_Identity=26.3546798029557, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=430, Percent_Identity=26.5116279069767, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71983106, Length=429, Percent_Identity=25.8741258741259, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71983099, Length=429, Percent_Identity=25.8741258741259, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=446, Percent_Identity=23.542600896861, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI193206654, Length=465, Percent_Identity=22.3655913978495, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17541374, Length=412, Percent_Identity=22.8155339805825, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583025, Length=466, Percent_Identity=24.0343347639485, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137668, Length=466, Percent_Identity=24.0343347639485, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24583023, Length=466, Percent_Identity=24.0343347639485, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281360483, Length=431, Percent_Identity=25.2900232018561, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17137666, Length=431, Percent_Identity=25.2900232018561, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281360481, Length=431, Percent_Identity=25.2900232018561, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45851; Mature: 45720

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLPAAGAAAPIRRTPIWNQLYVQVLVAIAVGGAIGHFWPETGEALKPLGDAFIKLVKM
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IIAPVIFLTVTTGIAGMRDIGKVGGVAAKAFGYFIVVSSFALVVGLVVANVVQPGAGMNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DPATLDAGAVQDYAAKSHEMTITGFLMDIIPATVAGAFVSGNILQVLFFSIPFGLALAMI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GERGEPALAVLNAVAEAFFRLVAIFMRAAPIGALGAFAFTVGRYGLESIVNLAALVGTFY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATSLIFVLVVLGAIARLHGFSILRLIRYLKEELLLVLGTSSSEAALPSLLEKMERAGAPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCC
TVVGLVVPTGYSFNLDGTNIYMTLAALFIAQAVGIDLPLGEQLLLLAVAMLSSKGAAGVT
EEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
GAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNFVGNAVATLVVSRWEGQLDR
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
QALEDALSGAPQPIAAPPVEADAD
HHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATLPAAGAAAPIRRTPIWNQLYVQVLVAIAVGGAIGHFWPETGEALKPLGDAFIKLVKM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
IIAPVIFLTVTTGIAGMRDIGKVGGVAAKAFGYFIVVSSFALVVGLVVANVVQPGAGMNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DPATLDAGAVQDYAAKSHEMTITGFLMDIIPATVAGAFVSGNILQVLFFSIPFGLALAMI
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GERGEPALAVLNAVAEAFFRLVAIFMRAAPIGALGAFAFTVGRYGLESIVNLAALVGTFY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATSLIFVLVVLGAIARLHGFSILRLIRYLKEELLLVLGTSSSEAALPSLLEKMERAGAPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCC
TVVGLVVPTGYSFNLDGTNIYMTLAALFIAQAVGIDLPLGEQLLLLAVAMLSSKGAAGVT
EEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
GAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNFVGNAVATLVVSRWEGQLDR
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
QALEDALSGAPQPIAAPPVEADAD
HHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]