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The map label for this gene is purB [H]

Identifier: 197104485

GI number: 197104485

Start: 1133439

End: 1134761

Strand: Reverse

Name: purB [H]

Synonym: PHZ_c1019

Alternate gene names: 197104485

Gene position: 1134761-1133439 (Counterclockwise)

Preceding gene: 197104486

Following gene: 197104484

Centisome position: 28.4

GC content: 70.29

Gene sequence:

>1323_bases
ATGATCCCCCGCTACACCCGCAAGGAAGCCGCCGACGTCTGGGCGCCCCAGACCCGCTTCAAGATCATGTTCGAAATCGA
GGCCCACGCCGCCGACAAGATGGCCGAGCTGGGCGTCATCCCCCGTGAGGCGGCGCGGGTGATCTGGGAGAAGGGCCGCA
ACGCGGTCTGGAACCCCGACCGGATCGACGAGATCGAGCGCGAGGTGAAGCACGACGTCATCGCCTTCCTCACCCACGTG
ACCGAGGTGGTGGGGCCGGAAGCCCGCTTCCTGCACCAGGGCATGACCAGCTCGGACGTCAACGACACCACCCTGGCGGT
GCAGCTGGTGCGGGCGACCGACCTGCTGCTCGAGGACGTGGACCTGGTGCTGGCGGCGCTGAAGCGCCGGGCCCTGGAGC
ACCGGATGACCCCCTGCGTCGGGCGCAGCCACGGCATCCACGCCGAGCCGACCACCTTCGGCCTGAAGCTCGCCGGCCAC
TACGCCGAGTTCCAGCGCGCGAAGGAGCGGCTGGCCATGGCGAAGTTCGAGATCGCCACCTGCGCCATCTCGGGCGCGGT
CGGCACCTTCGCCAACGTGGCCCCGGAGGTCGAGGCCTATGTCGCCGACAAGCTGGGCCTGGCGGTGGAGCCCGTCTCGA
CCCAGGTGATCCCGCGCGACCGGCACGCGGCCTATTTCGCGGCCCTGGCCGTGGTGGCCAGCTCGATCGAGCGCCTGGCC
GTCGAGATCCGCCACCTGCAGCGCACCGAGGTGCTGGAGGCCGAGGAGCCGTTCGACCCGGGCCAGAAGGGCTCGAGCGC
CATGCCGCACAAGCGCAACCCGATCCTGACCGAGAACCTGACCGGCCTGGCCCGCCTCGTCCGCTCGGCCGTGACGCCGG
CCATGGAGAACGTGGCGCTGTGGCACGAGCGGGACATCTCGCACTCGTCGGTCGAGCGCGGCATCTGCCCGGACGCCACG
GTCCACCTCGACTTCGCCCTGCGCCGGCTGGCCGGCGTGGTCGAGCGGCTGGTGGTCTACCCCGAAAACATGGCGAAGAA
CCTCGACCGCCTCGGCGGCCTGGTGCACTCCCAGCGGGTGCTGCTGGCCCTGACCCAGAAGGGGCTCAGCCGCGAGGCCA
GCTACGCGGCCGTCCAGCGCAACGCCATGAAGGTCTGGCGCGGCGAGGGCGACTTCCTGGGCTTCCTGAAGGCCGACCCC
GAGGTCACGCTGTCGGACGCCGAGCTCGAGCCGCTGTTCGACCTCGGCTACCACTTCAAGCACGTGGACACCGTCTTCGC
CCGGGTGTTCGGCGAACAAGGGAGCTTGACCGCCGCCGCGTAA

Upstream 100 bases:

>100_bases
CTGCACTACCTGCTGGCCGCCATCACCCTGCCGCGGGACATAGCGCGCGCCCGGCAAGGTCAGATTTGACATCCGGCCGG
CGCGCCCTCATTCCCGGGGC

Downstream 100 bases:

>100_bases
CCCAGCGTCACTTCCCTCGGATCGAGGGAGGACGACGATGGACATGCAAGGCATCCTGGCGCGCCAGAAGGCCGCTCACC
TGCGGGACGGCGCGCCGACG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MIPRYTRKEAADVWAPQTRFKIMFEIEAHAADKMAELGVIPREAARVIWEKGRNAVWNPDRIDEIEREVKHDVIAFLTHV
TEVVGPEARFLHQGMTSSDVNDTTLAVQLVRATDLLLEDVDLVLAALKRRALEHRMTPCVGRSHGIHAEPTTFGLKLAGH
YAEFQRAKERLAMAKFEIATCAISGAVGTFANVAPEVEAYVADKLGLAVEPVSTQVIPRDRHAAYFAALAVVASSIERLA
VEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVTPAMENVALWHERDISHSSVERGICPDAT
VHLDFALRRLAGVVERLVVYPENMAKNLDRLGGLVHSQRVLLALTQKGLSREASYAAVQRNAMKVWRGEGDFLGFLKADP
EVTLSDAELEPLFDLGYHFKHVDTVFARVFGEQGSLTAAA

Sequences:

>Translated_440_residues
MIPRYTRKEAADVWAPQTRFKIMFEIEAHAADKMAELGVIPREAARVIWEKGRNAVWNPDRIDEIEREVKHDVIAFLTHV
TEVVGPEARFLHQGMTSSDVNDTTLAVQLVRATDLLLEDVDLVLAALKRRALEHRMTPCVGRSHGIHAEPTTFGLKLAGH
YAEFQRAKERLAMAKFEIATCAISGAVGTFANVAPEVEAYVADKLGLAVEPVSTQVIPRDRHAAYFAALAVVASSIERLA
VEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVTPAMENVALWHERDISHSSVERGICPDAT
VHLDFALRRLAGVVERLVVYPENMAKNLDRLGGLVHSQRVLLALTQKGLSREASYAAVQRNAMKVWRGEGDFLGFLKADP
EVTLSDAELEPLFDLGYHFKHVDTVFARVFGEQGSLTAAA
>Mature_440_residues
MIPRYTRKEAADVWAPQTRFKIMFEIEAHAADKMAELGVIPREAARVIWEKGRNAVWNPDRIDEIEREVKHDVIAFLTHV
TEVVGPEARFLHQGMTSSDVNDTTLAVQLVRATDLLLEDVDLVLAALKRRALEHRMTPCVGRSHGIHAEPTTFGLKLAGH
YAEFQRAKERLAMAKFEIATCAISGAVGTFANVAPEVEAYVADKLGLAVEPVSTQVIPRDRHAAYFAALAVVASSIERLA
VEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVTPAMENVALWHERDISHSSVERGICPDAT
VHLDFALRRLAGVVERLVVYPENMAKNLDRLGGLVHSQRVLLALTQKGLSREASYAAVQRNAMKVWRGEGDFLGFLKADP
EVTLSDAELEPLFDLGYHFKHVDTVFARVFGEQGSLTAAA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=434, Percent_Identity=25.3456221198157, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI183227688, Length=386, Percent_Identity=26.1658031088083, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787376, Length=278, Percent_Identity=29.4964028776978, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=361, Percent_Identity=26.0387811634349, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32564234, Length=296, Percent_Identity=27.3648648648649, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323391, Length=389, Percent_Identity=28.5347043701799, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24647570, Length=326, Percent_Identity=26.9938650306748, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48704; Mature: 48704

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYTRKEAADVWAPQTRFKIMFEIEAHAADKMAELGVIPREAARVIWEKGRNAVWNPD
CCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCH
RIDEIEREVKHDVIAFLTHVTEVVGPEARFLHQGMTSSDVNDTTLAVQLVRATDLLLEDV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLVLAALKRRALEHRMTPCVGRSHGIHAEPTTFGLKLAGHYAEFQRAKERLAMAKFEIAT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANVAPEVEAYVADKLGLAVEPVSTQVIPRDRHAAYFAALAVVASSIERLA
HHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVTPAMENVAL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGICPDATVHLDFALRRLAGVVERLVVYPENMAKNLDRLGGLVHSQRV
HHHCCCCHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQKGLSREASYAAVQRNAMKVWRGEGDFLGFLKADPEVTLSDAELEPLFDLGYHFK
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHH
HVDTVFARVFGEQGSLTAAA
HHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MIPRYTRKEAADVWAPQTRFKIMFEIEAHAADKMAELGVIPREAARVIWEKGRNAVWNPD
CCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCH
RIDEIEREVKHDVIAFLTHVTEVVGPEARFLHQGMTSSDVNDTTLAVQLVRATDLLLEDV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLVLAALKRRALEHRMTPCVGRSHGIHAEPTTFGLKLAGHYAEFQRAKERLAMAKFEIAT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANVAPEVEAYVADKLGLAVEPVSTQVIPRDRHAAYFAALAVVASSIERLA
HHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVTPAMENVAL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGICPDATVHLDFALRRLAGVVERLVVYPENMAKNLDRLGGLVHSQRV
HHHCCCCHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQKGLSREASYAAVQRNAMKVWRGEGDFLGFLKADPEVTLSDAELEPLFDLGYHFK
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHH
HVDTVFARVFGEQGSLTAAA
HHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]