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The map label for this gene is yfgF [H]

Identifier: 197104413

GI number: 197104413

Start: 1042196

End: 1043749

Strand: Reverse

Name: yfgF [H]

Synonym: PHZ_c0947

Alternate gene names: 197104413

Gene position: 1043749-1042196 (Counterclockwise)

Preceding gene: 197104416

Following gene: 197104412

Centisome position: 26.12

GC content: 73.75

Gene sequence:

>1554_bases
ATGTCGCACCGGCTGCTGGGGTTCGCGTTCGCGGGGGCCGACCTCCTCCTGGAGGTGGGGCCGGACGCGCGCATCGCCTT
CGCGCTGGGCGCCAGCGAGACCCTCTCGGGCGCGGCCGAGACCTCGCTGGTCGGGCGCAGCTGGCGCCAGTTCGTGGACG
ACGCCGACCATGCGATGGTCGAGGCCCTGTTCGCCGGCCTGGATCCCGGCGCCCGCGCCGGCCCGGTGACCGTGCGGCTC
ACCCCCGGACAGGGGCACGGCCAGCGCCTGGCGGGCCTCGCCGCCTTCCGCCTGCCGCAGAACGGCGGCGCCATCTCCTG
CGCGCTCTCCCGGGTCAGCACGGGCGGGGTGGACGGCCTGCTCAGCCGCGCCGACTTCGAGGCCGCCGCGGCCCGGCTCG
CCGAGGATCCGGCGATGGAGATGGCTCTGATCGAACTGGAGGGCCTCGGCCGCCAGATCGAGGCGGCCGGCGAGGGTCGC
GACAAGCTGCTGGACCAGCTCACCGGAACGCTGCGCGCGCTGTCCCACCGGGGCGAGACCGCCGCCGAGCTGGGCGATGA
CCGCTACGCCCTCGTGCGCCGCCGGGGGGAGCCGGTCGAGGCGCTGCAGGACCGGCTGAAGAAGCTGATCGACGCCGACG
TGCAGCCCGCTGCCGGCGCCGTGGCCATGGGCGGGGTGGACGGCGCCAAGCAGGCGATGCGCGCCCTGCGCTTCGCCCTC
ACCAGCTTCATGGAGGGCGGCTTCGCCCAGATCCAGCCCGACAGCCTCAACCAGGCGCTGGACCAGGCCCTGGAGGGCGC
GCTCGACGACGCCCGCACGCTGAGCGCCACCATACGCGACCGGCAGTTCCGCCTGACCTACCAGCCCGTCGTGCGGCTGA
TGGACGGCCGGGTGAGCCACCACGAGGTGCTGGTCCGCTTCGGCGAGGAGGCCAGCCCCTTCCCGACGATCCGGGCGGCC
GAGGAGATGGATCTCATCGAGCCGCTGGACCTGGCCATCCTGACCGAGGCCCTGCGGGTGCTGGAGCGCGAGCCGAAGCA
CCGGCTGGCGGTGAACATCTCGGGCCGCACCATCGGCTCGGACGCCTTCGTCAGCCGGGCCATCGCGCTGCTGCGAGCCA
AGCCGGCGACCAGGGACCGGCTGGTGCTGGAGATCACCGAGAGCGCGGCCATCGACGACCTTGCCCAGGCGGACCGGCGG
CTCGCGGCCCTGCGCGAGGCGGGCTGCGAGATCTGCCTCGACGACTTCGGCGCCGGCGCGGCCTCGCTCGCCTACCTGCA
GCAACTGACCGTCGACATCGTGAAGATCGACGGGCGCTACATCCGCGAGCTGCAGCACGGCGGGCGCGAGAGCACCTTCA
TCCGGCACCTGGTGAACATGTGCCGCGAGCTGGGCGTCGAGACCCTGGCCGAGATGGTCGAGACCGCCGACGCCGAGCGC
GCCGTGCGGCTGGCCGGCGTCGACTACGCCCAGGGCTGGTTCTACGGCCCGGCCTCCGAGACCCTCGAGCTGAAGATCAA
GCCGCCCGCCATGCGACCCGCCCTCGCCCGATAG

Upstream 100 bases:

>100_bases
CGTAAGCCGGCGTAAACCATCTAGGGAAACCACGACTTAAGCCGGGCCGGCTACGGTCAAGCTTCGTCTACGACAGGATC
AGGCGCCCCACGTGGAGCTG

Downstream 100 bases:

>100_bases
CTTTCCCGATCACGAGGACTCGAACGGGCCGCCGCTAGCCAACGGCGCGAGCCGGGGCGTAGGGTTTTCCCATCGGGCGG
CGTTGGCCGGCCCAGTCGGT

Product: sensory box protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSHRLLGFAFAGADLLLEVGPDARIAFALGASETLSGAAETSLVGRSWRQFVDDADHAMVEALFAGLDPGARAGPVTVRL
TPGQGHGQRLAGLAAFRLPQNGGAISCALSRVSTGGVDGLLSRADFEAAAARLAEDPAMEMALIELEGLGRQIEAAGEGR
DKLLDQLTGTLRALSHRGETAAELGDDRYALVRRRGEPVEALQDRLKKLIDADVQPAAGAVAMGGVDGAKQAMRALRFAL
TSFMEGGFAQIQPDSLNQALDQALEGALDDARTLSATIRDRQFRLTYQPVVRLMDGRVSHHEVLVRFGEEASPFPTIRAA
EEMDLIEPLDLAILTEALRVLEREPKHRLAVNISGRTIGSDAFVSRAIALLRAKPATRDRLVLEITESAAIDDLAQADRR
LAALREAGCEICLDDFGAGAASLAYLQQLTVDIVKIDGRYIRELQHGGRESTFIRHLVNMCRELGVETLAEMVETADAER
AVRLAGVDYAQGWFYGPASETLELKIKPPAMRPALAR

Sequences:

>Translated_517_residues
MSHRLLGFAFAGADLLLEVGPDARIAFALGASETLSGAAETSLVGRSWRQFVDDADHAMVEALFAGLDPGARAGPVTVRL
TPGQGHGQRLAGLAAFRLPQNGGAISCALSRVSTGGVDGLLSRADFEAAAARLAEDPAMEMALIELEGLGRQIEAAGEGR
DKLLDQLTGTLRALSHRGETAAELGDDRYALVRRRGEPVEALQDRLKKLIDADVQPAAGAVAMGGVDGAKQAMRALRFAL
TSFMEGGFAQIQPDSLNQALDQALEGALDDARTLSATIRDRQFRLTYQPVVRLMDGRVSHHEVLVRFGEEASPFPTIRAA
EEMDLIEPLDLAILTEALRVLEREPKHRLAVNISGRTIGSDAFVSRAIALLRAKPATRDRLVLEITESAAIDDLAQADRR
LAALREAGCEICLDDFGAGAASLAYLQQLTVDIVKIDGRYIRELQHGGRESTFIRHLVNMCRELGVETLAEMVETADAER
AVRLAGVDYAQGWFYGPASETLELKIKPPAMRPALAR
>Mature_516_residues
SHRLLGFAFAGADLLLEVGPDARIAFALGASETLSGAAETSLVGRSWRQFVDDADHAMVEALFAGLDPGARAGPVTVRLT
PGQGHGQRLAGLAAFRLPQNGGAISCALSRVSTGGVDGLLSRADFEAAAARLAEDPAMEMALIELEGLGRQIEAAGEGRD
KLLDQLTGTLRALSHRGETAAELGDDRYALVRRRGEPVEALQDRLKKLIDADVQPAAGAVAMGGVDGAKQAMRALRFALT
SFMEGGFAQIQPDSLNQALDQALEGALDDARTLSATIRDRQFRLTYQPVVRLMDGRVSHHEVLVRFGEEASPFPTIRAAE
EMDLIEPLDLAILTEALRVLEREPKHRLAVNISGRTIGSDAFVSRAIALLRAKPATRDRLVLEITESAAIDDLAQADRRL
AALREAGCEICLDDFGAGAASLAYLQQLTVDIVKIDGRYIRELQHGGRESTFIRHLVNMCRELGVETLAEMVETADAERA
VRLAGVDYAQGWFYGPASETLELKIKPPAMRPALAR

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=415, Percent_Identity=26.5060240963855, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081743, Length=233, Percent_Identity=32.1888412017167, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1790496, Length=246, Percent_Identity=30.0813008130081, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788502, Length=237, Percent_Identity=31.6455696202532, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081845, Length=258, Percent_Identity=29.0697674418605, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI226510982, Length=253, Percent_Identity=31.6205533596838, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787541, Length=412, Percent_Identity=24.2718446601942, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082096, Length=285, Percent_Identity=25.6140350877193, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786507, Length=234, Percent_Identity=26.4957264957265, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081980, Length=235, Percent_Identity=26.8085106382979, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081921, Length=239, Percent_Identity=26.7782426778243, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787055, Length=360, Percent_Identity=22.7777777777778, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 55582; Mature: 55451

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRLLGFAFAGADLLLEVGPDARIAFALGASETLSGAAETSLVGRSWRQFVDDADHAMV
CCCCEEEEEEECCEEEEEECCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHCCHHHHHH
EALFAGLDPGARAGPVTVRLTPGQGHGQRLAGLAAFRLPQNGGAISCALSRVSTGGVDGL
HHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHEECCCCCCEEHHHHHHHCCCCHHHH
LSRADFEAAAARLAEDPAMEMALIELEGLGRQIEAAGEGRDKLLDQLTGTLRALSHRGET
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
AAELGDDRYALVRRRGEPVEALQDRLKKLIDADVQPAAGAVAMGGVDGAKQAMRALRFAL
HHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
TSFMEGGFAQIQPDSLNQALDQALEGALDDARTLSATIRDRQFRLTYQPVVRLMDGRVSH
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCHH
HEVLVRFGEEASPFPTIRAAEEMDLIEPLDLAILTEALRVLEREPKHRLAVNISGRTIGS
HHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
DAFVSRAIALLRAKPATRDRLVLEITESAAIDDLAQADRRLAALREAGCEICLDDFGAGA
HHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCH
ASLAYLQQLTVDIVKIDGRYIRELQHGGRESTFIRHLVNMCRELGVETLAEMVETADAER
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHH
AVRLAGVDYAQGWFYGPASETLELKIKPPAMRPALAR
HHHHCCCCHHCCEEECCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SHRLLGFAFAGADLLLEVGPDARIAFALGASETLSGAAETSLVGRSWRQFVDDADHAMV
CCCEEEEEEECCEEEEEECCCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHCCHHHHHH
EALFAGLDPGARAGPVTVRLTPGQGHGQRLAGLAAFRLPQNGGAISCALSRVSTGGVDGL
HHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHEECCCCCCEEHHHHHHHCCCCHHHH
LSRADFEAAAARLAEDPAMEMALIELEGLGRQIEAAGEGRDKLLDQLTGTLRALSHRGET
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
AAELGDDRYALVRRRGEPVEALQDRLKKLIDADVQPAAGAVAMGGVDGAKQAMRALRFAL
HHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
TSFMEGGFAQIQPDSLNQALDQALEGALDDARTLSATIRDRQFRLTYQPVVRLMDGRVSH
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCHH
HEVLVRFGEEASPFPTIRAAEEMDLIEPLDLAILTEALRVLEREPKHRLAVNISGRTIGS
HHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
DAFVSRAIALLRAKPATRDRLVLEITESAAIDDLAQADRRLAALREAGCEICLDDFGAGA
HHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCH
ASLAYLQQLTVDIVKIDGRYIRELQHGGRESTFIRHLVNMCRELGVETLAEMVETADAER
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHH
AVRLAGVDYAQGWFYGPASETLELKIKPPAMRPALAR
HHHHCCCCHHCCEEECCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]